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Citations for Qiagen :
351 - 400 of 2105 citations for Potassium Bromate 90 95% Chemical Purity 18O3 98% 100 Ug Ml In 18O Water since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The cultures were pelleted and genomic DNA was extracted into DNase free water using the DNeasy Blood and Tissue Kit (Qiagen). gDNA concentrations were determined with the Qubit 1X dsDNA High-Sensitivity Kit and fluorometer (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Isolated colonies were suspended in PCR-grade water for the following automated DNA extraction using QIAcube and QIAmp mini kit (QIAGEN). The DNA extracts were used for SBT ...
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bioRxiv - Microbiology 2023Quote: ... Filters were snap-frozen using liquid nitrogen and stored at -80 °C until DNA extraction with the DNeasy Power Water kit (Qiagen) using the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... The pellet was washed once with 0.1% peptone water and subjected to total DNA extraction with a QIAamp PowerFecal Pro DNA Kit (Qiagen, Germany) following manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... cDNA was diluted to 5 ng/µl in RNase-free water and 1 µl used for real-time qPCR with Qiagen QuantiNova SYBR Green Master Mix (Qiagen) in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: BC3 cells were treated with indicated chemicals for 65h and total DNA was purified from cell lysates using the FlexiGene DNA kit (Qiagen). Intracellular viral DNA was measured by real-time quantitative (q ...
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bioRxiv - Neuroscience 2020Quote: ... Antennal fragments were rinsed off the pestle with 100 µl PBS and 40 μl EDTA (100 mM) and 20 μl RNase (12.5 μg/μl) (Qiagen 19101) were added to the antennal lysate ...
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bioRxiv - Immunology 2019Quote: Host DNA was quantitated from 100 mg of luminal content (100 mg) from small intestine by using QIAamp DNA Stool Mini kit (Qiagen), and subjected to quantitative PCR using QuantiFast SYBR Green PCR kit (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... We co-transfected 100 ng of psiCheck-23xant-miR275 and the synthetic tsetse miR275miScript miRNA mimic at 100 nM (Qiagen) or with AllStars Negative Control (Qiagen ...
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bioRxiv - Genomics 2022Quote: ... were annealed (10 μL each 100 μM) to 10 μL 5′-[Phos]-CTGTCTCTTATACACATCT-3’ oligonucleotide (100 μM) within 80 μL EB buffer (Qiagen), incubating 2 min at 95°C and cooled to room temperature (0.1°C/sec) ...
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bioRxiv - Molecular Biology 2023Quote: ... and after two washes was eluted with 100 μl of freshly prepared 100 mM DTT and then purified using RNeasy MinElute Cleanup kit (QIAGEN). 4sU-RNA was quantified with the Quit RNA High Sensitivity kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were incubated with 100 µL DTT (100 mM) at RT for 5 min and recovered by the RNeasy clean up kit (Qiagen) using the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... the filtration columns were equilibrated (QIAGEN-tip 100) with 15 ml of buffer QBT (Equilibration Buffer) ...
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bioRxiv - Cell Biology 2021Quote: 100 μL of Ni-NTA Agarose resin (Qiagen) per condition was equilibrated with binding buffer (10 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... we used Genomic-tip 100/G columns (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and eluted into 100 μl AE buffer (Qiagen). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.5 µl of diluted template cDNA (1:3, nuclease-free water) per real-time PCR reaction (10 µl – SYBR Green PCR kit, Qiagen, 204143) was used to assay specific transcript abundance (CFX96 Real-Time System ...
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bioRxiv - Microbiology 2020Quote: ... and the pellet containing virions was resuspended in 200 μl UltraPure water and added to the QIAGEN DNeasy PowerSoil Kit bead tubes (QIAGEN, Germany) for DNA extraction according to the manufacturer’s instructions with one exception ...
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bioRxiv - Genetics 2020Quote: ... The RNA pellet was eluted in 15 μL of nuclease-free water and quantified on a Nanodrop 1000 spectrophotometer (NanoDrop Technologies) prior to DNAse treatment with QuantiTect® Reverse Transcription kit (Qiagen). The quality of the RNA was examined by electrophoresis on a 1% agarose gel (Sigma Aldrich) ...
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bioRxiv - Genomics 2021Quote: ... The RNA pellets were subsequently dissolved in an appropriate volume of RNase free water and treated with RNase-Free DNase (Qiagen, 79254) by incubation at room temperature for 10 min to avoid genomic DNA contamination ...
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bioRxiv - Genomics 2022Quote: ... The RNA pellet was eluted in 15 μL of nuclease-free water and quantified on a Nanodrop 1000 spectrophotometer (NanoDrop Technologies) prior to DNAse treatment with QuantiTect® Reverse Transcription kit (Qiagen). The quality of the RNA was examined by electrophoresis on a 1% agarose gel (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... we used low-binding plastics and added 1 µl of 0.1 ng/µL tRNA carrier (i.e. spike water, Qiagen, Cat. No. 1068337) in each tube with stock eluted DNA ...
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bioRxiv - Microbiology 2023Quote: ... two negative PCR controls (further termed as no PCR template controls, NTCs; RNA free water; Cat. No. 760011596, Qiagen, Hilden, Germany) were used ...
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bioRxiv - Microbiology 2023Quote: ... RNA pellets were dissolved in 88 μL of nuclease-free water and subjected to genomic DNA digestion with DNase (Qiagen Inc.). RNA samples were then concentrated using RNA Clean & Concentrator (Zymo Research) ...
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bioRxiv - Plant Biology 2023Quote: ... leaf-discs were collected and ground in autoclaved Milli-Q water using a TissueLyser II (QIAGEN; 45 seconds at 30 Hz). Serial dilutions of the ground tissue were spotted onto low salt Luria Bertani (LB ...
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bioRxiv - Microbiology 2023Quote: ... cells were collected by centrifugation and quickly washed with ice-cold water to remove the methanol and resuspended in RNAlater solution (Qiagen, 76104) for at least 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... elastase (3.125 U/mL, Serva) and DNAse (17 U/mL, Qiagen) for 20 min at 37°C while shaking (750 rpm) ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of the 2-mL culture was removed and replaced by 1 mL of RNAprotect Cell Reagent (Qiagen). After 5 min of incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... doxycycline was added at (0.1 μg/ml, 1 μg/ml (CHRDL2 +) or 10 μg/ml (CHRDL2 ++) RNA was extracted (RNeasy, QIAGEN) and quantified by real-time reverse transcriptase polymerase chain reaction (qPCR ...
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bioRxiv - Developmental Biology 2022Quote: ... injected embryos developed to blastocyst stage in culture were collected in 1.5 ul water individually and the whole genome was replicated using a REPLI-g® Mini Kit (Qiagen, Germantown, MD) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... We then suspended the pellet in RNAse-free water and purified it using a Qiagen RNeasy MinElute cleanup kit (Qiagen, cat. #74204) following standard protocols.
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bioRxiv - Cell Biology 2021Quote: ... We then suspended the pellet in RNAse-free water and purified it using a Qiagen RNeasy MinElute cleanup kit (Qiagen, cat. #74204) following standard protocols.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Aqueous homogenates of ovarian tissue samples were created by weighing the tissue and adding 4uL of water per mg of tissue and then using a bead beater (TissueLyser II, QIAGEN; Germantown, MD) to homogenize the sample ...
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bioRxiv - Cell Biology 2022Quote: Snap-frozen iBAT samples were homogenized in deionized water (200 µl per 10 mg tissue) using a TissueLyser bead mill (85210, Qiagen, Hilden, Germany). The glycogen content of the homogenates was measured using a Glycogen Assay Kit II (ab169558 ...
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bioRxiv - Pathology 2020Quote: ... The cDNA was first diluted with nuclease-free water and then added to the RT2 SYBR Green ROXTM qPCR Mastermix (Qiagen, Hilden, Germany) according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2021Quote: ... The liquid was aliquoted into a microcentrifuge tube with 590 µl nuclease-free water and 10 µl Proteinase K (QIAGEN, Hilden, Germany). For RNA extraction from swabs ...
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bioRxiv - Microbiology 2023Quote: ... in-house mock community (25 diverse Gram-positive and Gram-negative bacteria and fungi) and a negative reagent control (nuclease-free water, Qiagen, Hilden, Germany) were included ...
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bioRxiv - Microbiology 2023Quote: ... The RNA samples were resuspended in RNase-free water before clean-up treatment with the RNase-Free DNase Set (Qiagen, Hilden, Germany) following the manufacturer’s protocol for off-column clean-up ...
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bioRxiv - Microbiology 2023Quote: ... we dried both washed lichen fragments and wash-water pellets at 65°C and extracted DNA with the DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany).
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bioRxiv - Microbiology 2020Quote: ... was extracted from 100 μl of viral suspension complemented with 100 μl of 1x PBS with the QIAamp DNA Mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions for DNA purification of total DNA from cultured cells ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from approximately 250 g cecal and 100 g colonic content using the DNeasy PowerLyzer PowerSoil kit (Qiagen, 12855-100), as described previously3 ...
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bioRxiv - Immunology 2022Quote: ... Total DNA was isolated from approximately 200 mg frozen tissue by repeated bead-beating combined with chemical lysis and column-based purification using the QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions (Salonen et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... Cleared lysate was applied to a 20 ml disposable gravity-flow column 1.5 ml (0.75 ml bed volume) of NI-NTA agarose (Qiagen cat. #30210), washed twice in three bed volumes of Lysis Buffer ...
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bioRxiv - Microbiology 2019Quote: ... from the ME phase and ES phase (1 ml) were added to 4 ml and 2 ml of RNAprotect Bacteria Reagent (Qiagen), respectively ...
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bioRxiv - Genetics 2023Quote: ... To prepare the protein column we loaded 2 mL (1 mL column volume) of Nickel NTA resin onto the 5 mL Polypropylene columns from Qiagen and the resin buffer was allowed to drain ...
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bioRxiv - Microbiology 2021Quote: ... with QIAGEN-tip 100 (Qiagen, Cat No./ID: 10043) following the manufacturer’s instruction with the following minor modifications ...
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bioRxiv - Microbiology 2021Quote: ... The Qiagen Genomic Tip (100/G) kit (Qiagen; 10243) was used to extract DNA from 100 mg of tissue powder ...
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bioRxiv - Genomics 2022Quote: ... and eluted with 100 μl 1X AE Buffer (Qiagen). For enrichment by MDA ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was removed by 100 µg RNaseA (Qiagen, # 19101) treatment at 37°C for 1 hr ...
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bioRxiv - Microbiology 2019Quote: ... then 100 μl of RNA protect bacteria reagent (Qiagen) was added ...