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Citations for Qiagen :
351 - 400 of 10000+ citations for Mouse Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... pH was adjusted to 8 after resuspension to allow the binding of 6x His-tagged toxins to Ni-NTA agarose resin (reference: 30210; Qiagen). Resulting samples were passed through chromatography columns containing the Ni-NTA agarose resin and were eluted with denaturing buffer containing increasing concentrations of Imidazole (10 mM ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The remaining supernatant was mixed with 13x its volume of binding buffer and DNA was purified with MinElute columns (Qiagen), following the manufacturer’s instructions with the exception of a 15-minute incubation at 37□°C during the elution step ...
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bioRxiv - Cancer Biology 2024Quote: ... the clarified lysate was diluted into 1x Ni-NTA binding buffer and bound to equilibrated Ni-NTA resin (Qiagen, 30210) for 20 minutes at 4°C with end-over-end mixing ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using mouse anti-His antibody from Qiagen (Cat No./ID: 34660 1:500). Anti-mouse HRP was used for colorimetric development (Cat No./ID-A8924 1:1000) ...
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bioRxiv - Developmental Biology 2020Quote: We isolated RNA from single mouse embryonic hearts at embryonic stage E12.5 by using the RNeasy Kit (#74104; Qiagen) and the RNase-Free DNase Set (#79254 ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from mouse brains with TRIzol or from selected cells with RNeasy Plus Mini Kit (Qiagen) and reverse-transcribed ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted either from mouse total lung tissue after homogenization using the RNeasy Mini Kit (Qiagen GmbH) or from enriched mouse lung CD4+ T cells (CD4 T cell isolation kit ...
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bioRxiv - Bioengineering 2020Quote: Genomic DNA from mouse tissues and cultured HEK293 cells were extracted using DNeasy Blood & Tissue Kit (Qiagen, Germantown, MD). Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research ...
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bioRxiv - Neuroscience 2020Quote: RNA from rat Schwann cell cultures or mouse nerve tissue was extracted using an RNeasy Micro Extraction Kit (Qiagen). RNA quality and concentration was determined after extraction using a nanodrop 2000 machine (Thermo) ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was extracted from blood and mouse lung tissues using a Blood and Tissue DNA kit (Qiagen, Germantown, MD). Rat and mouse DNA were quantified by RT-qPCR using rat- and mouse-specific primers included in Supplementary Table 1 (Walker et al ...
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bioRxiv - Immunology 2022Quote: ... The recombinant plasmids for mouse vaccination were prepared using an EndoFree Plasmid Purification Kit (Cat# 12391, Qiagen, Hilden, USA).
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bioRxiv - Molecular Biology 2021Quote: ... and genomic DNA from MEFs and mouse tissue samples were extracted using the DNeasy Blood and Tissue Kit (Qiagen), according to the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from cultured cells or mouse kidneys using the total RNA extraction miRNeasy mini kits (Qiagen). cDNA synthesis was performed using the iScript cDNa synthesis kit (Bio-rad) ...
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bioRxiv - Microbiology 2020Quote: RNA from hNE apical washes and mouse oropharyngeal swabs were prepared using QIAamp® Viral RNA Mini Kit (QIAGEN) and Nucleospin® RNA kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2022Quote: ... total RNA was isolated from E11.5 wildtype and humanized mouse dorsolateral telencephalon using the RNAeasy Micro Plus Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was generated from 0.1 grams of feces from each mouse using the power soil DNA kit (QIAGEN) according to the manufacturer’s instructions except that cells were broken by a bead beater instead of a vortex ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was isolated from pooled E12.5 CD1 mouse embryos using an RNeasy® Plus Mini Kit (Qiagen, Hilden, Germany) and then used to generate cDNA with a QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was extracted from a pool of 500 MN per mouse with the microRNeasy kit according to manufacturer’s instruction (Qiagen). RNA was quantified with a Qubit (fluorimeter from Life Technologies ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA was extracted from human and mouse feces-derived bacterial supernatant using the DNeasy powersoil kit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RNA was extracted from the midbrain of each mouse using the RNeasy® Plus Universal Mini Kit (Qiagen, Germany). The reverse transcription was performed using 300 ng of total RNA mixed with 3.8 μM of oligo(dT)20 (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was isolated from mouse fecal samples following the standard protocol from the DNeasy PowerSoil Kit (Qiagen). Sequencing libraries were generated according to methods adapted from Caporaso et al ...
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bioRxiv - Molecular Biology 2024Quote: ... For Southern blotting or qPCR total DNA was isolated from mouse tissues using the DNeasy Blood & Tissue Kit (QIAGEN) as previously described(Kühl et al. ...
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bioRxiv - Microbiology 2024Quote: Bacterial DNA was extracted from sorted cells and mouse fecal pellets using the AllPrep PowerFecal DNA/RNA kit (Qiagen) as previously described.13 Extracted DNA underwent library preparation and 16S rRNA gene sequencing of the V4-V5 hypervariable region with 515F/926R primers using the Illumina MiSeq PE250 system at the Université de Québec à Montréal (UQAM ...
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bioRxiv - Pathology 2023Quote: Total RNA was isolated from mouse liver tissues according to the manufacturer’s instructions (RNeasy® Mini Kit, 74106, Qiagen). RNA samples were incubated with DNase I (79254 ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein-protein interaction networks were built using Ingenuity Pathway Analysis (IPA) (Qiagen).
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bioRxiv - Cell Biology 2019Quote: ... The copy number of standard DNA molecules was then determined by the following formula (from the script “Critical Factors for Successful Real Time PCR”, Qiagen):
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bioRxiv - Immunology 2020Quote: Differentially activated biological pathways and upstream regulators were assessed for model factors of interest using the Ingenuity Pathway Analysis software (IPA; QIAGEN Inc. ...
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LSD1 inhibition improves efficacy of adoptive T cell therapy by enhancing CD8+ T cell responsivenessbioRxiv - Immunology 2023Quote: ... Pathway analysis and transcription factor prediction analysis were performed with QIAGEN’s Ingenuity Pathway Analysis (IPA, QIAGEN Redwood City, www.qiagen.com/ingenuity).
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bioRxiv - Cell Biology 2019Quote: ... Input (3% of total binding reaction) and bound samples (50% of binding reaction) were subjected to SDS-PAGE followed by immunoblot analysis with His (Qiagen 34660) and GST (BioLegend MMS-112P ...
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bioRxiv - Genetics 2019Quote: ... The whole body of a single insect was homogenized in a PCR well containing 50 μl of the Lysis & Binding Solution and zirconia beads (ø 2 mm; Nikkato) in TissueLyser II (QIAGEN) at 25 Hz for 30 s ...
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bioRxiv - Biophysics 2021Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the two assay plasmids by temperature-cycle ligation.
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bioRxiv - Molecular Biology 2020Quote: RNA and protein were extracted from cell lines using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA, USA). Total RNA was also DNase treated using the TURBO DNA-free Kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: RNA was extracted simultaneously from the same tissue pieces used for DNA extraction using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... protein samples were processed further according to the Western Blot protocol and RNA was extracted using an RNAeasy Kit (Qiagen).
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bioRxiv - Cell Biology 2021Quote: Genomic DNA from 1×106 FACS sorted p7 MPs from one animal was isolated with the All Prep DNA / RNA / Protein Mini Kit (Qiagen) and used as one sample ...
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bioRxiv - Cell Biology 2021Quote: ... Genomic DNA of clones showing depletion of spartin protein by immunoblot analysis with the spartin antibody were extracted (DNeasy Blood and Tissue Kit, Qiagen), and the genomic DNA sequence surrounding the target exon of spartin was amplified by PCR (sense ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA from CRISPR-on HEK293T cells and control cells were purified using a AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Then ...
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bioRxiv - Biochemistry 2022Quote: ... Metal-chelate affinity chromatography was used to purify the 6xHis-tagged proteins using the Ni-NTA Fast Start Kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA was extracted from AAV1-transduced primary hippocampal neurons (DIV12-14) using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Total RNA was polyA selected and sequenced (Hudson Alpha GSL ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at low speed (~1000 RPM) and lysed with one mL RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen).
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bioRxiv - Cancer Biology 2022Quote: ... 250,000 cells were lysed in RLT-plus buffer and RNA was purified with AllPrep DNA/RNA/Protein Mini Kit (#80004, Qiagen) according to the manufacturer protocol ...
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bioRxiv - Systems Biology 2022Quote: The co-immunoprecipitated RNA samples were submitted to protein digestion using proteinase K (Fermentas) and purified using the MinElute Reaction Cleanup Kit (QIAGEN) with a DNase treatment step ...
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bioRxiv - Bioengineering 2023Quote: ... The bands representing the protein hybrid libraries were excised and DNA was purified using the QIAquick Gel Extraction Kit (Qiagen). The DNA concentration was then measured with the Quant-iT dsDNA (HS ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from cell lysates of bovine granulocytes and sorted PBMC subsets of three to seven Holstein Friesian cattle using the AllPrep RNA/Protein kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the AbundancePCA plasmid and BindingPCA plasmids by temperature-cycle ligation ...
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bioRxiv - Microbiology 2023Quote: All proteins from previously frozen samples (tumors and healthy parts of murine colon) were extracted using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Protein concentrations were estimated using NanoDrop A280 absorbance ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and proteins were extracted from fresh or snap-frozen FACS-sorted 2-5 million splenic B cells using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Protein Precipitation Solution (Qiagen) was added at 0.33x and mixed well ...
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bioRxiv - Cell Biology 2022Quote: To synthesize cDNA from 1 μg RNA the Quantitect kit (Qiagen) was used according to manufacturer’s instructions ...