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Citations for Qiagen :
351 - 400 of 2297 citations for Mouse Anti HIV 1 p24 Recombinant Antibody clone 183 H12 5C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: RNA isolation from the MIA PaCa-2 clones was performed by using the RNeasy Plus Micro Kit (QIAGEN). Libraries for RNA-seq were made with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we grew single clones in monoculture with appropriate antibiotics overnight and extracted genomic DNA (QIAGEN Genomic DNA Kit) for library preparation with the Nextera XT Library Preparation Kit ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from U2OS WT and ZNF524 KO clones using QIAamp DNA Blood Mini Kit (Qiagen) with RNase treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA from ~ 20 positive clones for each cell line was isolated using QIAprep Spin Miniprep Kit (Qiagen) and the cloned rDNA promoter region was sequenced using Sanger sequencing ...
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bioRxiv - Microbiology 2022Quote: ... and RT2 profiler PCR Arrays: Mouse Antiviral response (Qiagen). Data analyses were performed using the GeneGlobe (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: Mouse MSCs RT2 Prolifer PCR array (PAMM-084ZG, Qiagen) was used to evaluate the expression of 84 specific genes related to autophagy using manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... mouse liver was purified using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions and combined for CHANGE-seq as previously described.37 Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse tissue was homogenised with a TissueLyser II (Qiagen) at 30 Hz for 60 seconds ...
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bioRxiv - Immunology 2022Quote: ... RT2 Profiler™ PCR array mouse cytokines & chemokines (Qiagen) was used according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Whole mouse kidneys were homogenized in lysis buffer (Qiagen) and total RNA was extracted from a portion of the lysate using RNeasy Mini kit ...
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bioRxiv - Developmental Biology 2022Quote: ... A mouse neurogenesis PCR array kit (#PAMM-404AZA, Qiagen) was employed to measure the mRNA expression of genes involved in this pathway using real-time PCR (ABI 7500) ...
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bioRxiv - Genetics 2022Quote: ... using biotinylated His5-antibody (Qiagen), as previously described (Asbury et al. ...
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bioRxiv - Bioengineering 2020Quote: ... the membrane was incubated with freshly prepared primary antibody solution (1:1,000 dilution for anti-Alix [ab117600, Abcam], anti-Tsg101 [ab30871, Abcam], anti-Calnexin [ab22595, Abcam], anti-His [34660, Qiagen] ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: We extracted DNA from trametinib-resistant clones and their parental counterparts using a DNeasy Blood & Tissue Kit (Qiagen, 69506). DNA was submitted for profiling on the MSK-IMPACT (Integrated Mutation Profiling of Actionable Cancer Targets ...
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bioRxiv - Genomics 2021Quote: RNA was extracted from validated clones and wild-type HAP1 cells using the RNeasy Mini Kit (Qiagen cat # 74104) following the manufacturer’s instructions and then treated with TURBO DNA-free™ Kit (Life Technologies cat # AM1907 ...
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bioRxiv - Immunology 2023Quote: ... 24 hours) treated and untreated control U2OS WT and ZBTB48 KO clones using the RNeasy Plus Mini Kit (Qiagen) with additional on-column DNaseI digestion following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The sampled cellular mass from each clone was transferred into a lysis buffer (DNeasy® Plant Mini Kit, QIAGEN), and cells were disrupted by 1.5-min vortexing (Beadbeater ...
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bioRxiv - Immunology 2024Quote: ... DNA was isolated from STX4 KO clones and BEAS-2B.Cas9 control cells using the QIAamp DNA Micro Kit (Qiagen) per manufacturer’s protocols ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNAs of positive clones were propagated and purified using the standard Qiagen maxiprep plasmid purification kit (#12163, Qiagen). HEK-293 cells (ATCC type ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA from each OCT4 knockout clone or WT was extracted using DNeasy blood and tissue kit (QIAGEN, 69504) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 1X107 cells from each single clone were used for RNA extraction with RNeasy Plus Mini Kit (Qiagen, catalog#, 74134). cDNA was synthesized using SuperScript™ III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: Genomic DNA was extracted using either DNazol (for the rtlspac1 clone 1B5) or Qiagen Genomic DNA kit (Qiagen 13323).
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bioRxiv - Immunology 2020Quote: ... Mouse and Human IFN I RT2 Profiler PCR Arrays (Qiagen) were performed and relative expression determined using the ∆∆CT method and normalized for 5 housekeeping genes according to manufacturer’s guidance.
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bioRxiv - Developmental Biology 2021Quote: ... Total mouse RNA was isolated using RNeasy mini kit (Qiagen) and reverse transcribed to cDNA using an oligo (dT ...
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bioRxiv - Cell Biology 2021Quote: ... Frozen mouse liver were first homogenized using TissueLyser II (Qiagen) and then lysed in 1X Laemmli buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... RT² Profiler™ PCR Array Mouse Neurogenesis (330231, Qiagen, Germany) was performed according to the manufacturer’s instructions on LightCycler 96 (Roche ...
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bioRxiv - Immunology 2022Quote: ... or from frozen mouse BMDMs using RNeasy Mini kit (Qiagen). Reverse transcription (RT ...
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bioRxiv - Physiology 2023Quote: ... RT2 Profiler PCR Array for Mouse Fibrosis (PAMM-120Z, Qiagen) and Qiagen GeneGlobe Analysis was used to evaluate fibrosis between normal saline and experimental groups at each time point.
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bioRxiv - Developmental Biology 2024Quote: ... mouse embryos were injected with two siRNAs (Qiagen, S104403294 & S104403287) against ERH at a total concentration of 400 nM ...
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bioRxiv - Biochemistry 2023Quote: ... The PentaHis antibody (Qiagen, Düsseldorf, DE) against His-Tagged proteins was diluted 1:5000 in TBS buffer containing 3% BSA and the membrane was incubated overnight at 4°C followed by 2×10 min washing steps in TBST and 1×10 min in TBS buffers ...
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bioRxiv - Cell Biology 2020Quote: One colony per clone corresponding to ∼2×104 cells and 2×103 primary cells of each individual were lysed in RLT Plus (Qiagen) and stored at −80°C until processing ...
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bioRxiv - Cell Biology 2020Quote: ... Mutant alleles in knockout clones were assessed by sequencing of genomic DNA extracted using the QIAamp DNA Mini kit (Qiagen). The target locus was amplified using flanking primers (see Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Positive clones were cultured overnight in 5mL LB broth plus Ampicillin and the constructs were purified by mini-prep (Qiagen). Enhancer inserts were sequenced using Citrine_Fwd (5’-TGTCCCCAGTGCAAGTGC-3’ ...
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bioRxiv - Immunology 2020Quote: ... and DNA from 7-10 clones from before and after FLPo-mediated recombination was prepared by miniprep (Qiagen, cat. 27106) and sequenced by Sanger sequencing with the primer GFP int F Age 66 ...
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bioRxiv - Biochemistry 2021Quote: ... resistant clones were isolated and subjected to tunicamycin cytotoxicity assays and gDNA extraction using the Gentra Puregene Tissue Kit (Qiagen). Paired end ...
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bioRxiv - Cancer Biology 2021Quote: ... specific to the DNA sequence (or cDNA sequence from reverse transcribed clone G2 RNA samples (QuantiTect Reverse Transcription Kit, Qiagen)) for PCR (PCR SuperMix High Fidelity ...
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bioRxiv - Microbiology 2021Quote: ... the presence of both parental antibiotic resistance markers was validated by PCR on DNA extracted from hybrid clones (DNeasy blood and Tissue Kit, Qiagen), targeting the antibiotic resistance genes (see Sup ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Between 20 and 30 clones per individual were selected randomly and purified using the QIAprep Spin Miniprep Kit (QIAGEN GmbH) in order to cover several times each represented allele ...
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bioRxiv - Genetics 2022Quote: ... DNA was extracted from a wild type clone and a mutant using a QIAGEN Plasmid Midi Kit (Qiagen, Tokyo, Japan) and used as standard DNA to determine ratios of mosaicism ...
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bioRxiv - Genomics 2021Quote: Genomic DNA from the BPK282 cl4 and BPK081 cl8 clones was extracted in bulk using the QIAmpTM DNA Mini kit (Qiagen) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: The validated Alx1DN (N-terminal portion of protein product containing homeodomain and nuclear localization domains) clones in pCS2+8 were purified via miniprep (Qiagen) alongside a control (C-terminal portion containing transactivation domain) ...
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bioRxiv - Microbiology 2022Quote: ... One to three parasite clones from each time point were propagated for genomic DNA extraction by DNeasy Blood & Tissue Kits (Qiagen) for whole-genome sequencing on a Hiseq X (Illumina).
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bioRxiv - Immunology 2024Quote: ... of either the B cell clone or polyclonal B cells of the patients were directly FACS sorted into 100µL ALT lysis buffer (Qiagen MicroKit). gDNA was extracted as per manufacturer’s instructions (Qiagen MicroKit) ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA from HuCCT1 pcDNA3.1 (empty vector) and pcLINC00313 over-expressing clones was isolated using the miRNeasy kit (Qiagen, 217004). RNA-seq was performed by Active Motif ...
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bioRxiv - Genetics 2022Quote: ... A small number of positive clones were expanded and PCR genotyping was repeated on gDNA isolated using the DNeasy Blood and Tissue Kit (Qiagen) or GeneJET Genomic DNA Purification Kit (Thermo) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Predicted mutations and unassigned missing coverage/ new junction evidence were curated manually for each sequenced clone using CLC Genomics Workbench 11 (Qiagen). To generate plasmid comparisons we used the visualization tool Easyfig (v2.2.2 ...
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bioRxiv - Microbiology 2022Quote: ... FACS-sorted d2EGFP+ and d2EGFP− cells of d2EGFP TBX4B and d2EGFP TBW 11.50 clones using RNeasy® plus mini kit (Qiagen). cDNA was synthesised from RNA using transcriptor first strand cDNA synthesis kit (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... All plasmid clones were prepared for sequence analysis using the QIAGEN QIAprep kit according to the instructions of the supplier (QIAGEN). From pDONR221 clones ...
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bioRxiv - Cell Biology 2024Quote: ... Individual organoid clones were expanded in parallel and genomic DNA was isolated using a DNeasy Blood & Tissue kit (Qiagen, 69506). 35 cycle PCR reactions with an input of 100 ng of gDNA and an annealing temperature of 58°C were performed using primers flanking the sgAscl1 edit site and KAPA mouse genotyping kit following manufacturer protocols (Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lines and mouse tails using DNeasy Blood & Tissue kit (Qiagen). Exome libraries were prepared by the Advanced Sequencing Facility at the Crick and sequenced using an Illumina HiSeq 4000 using 100 base pair paired-end reads ...