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Citations for Qiagen :
351 - 400 of 678 citations for Fmoc Rink Amide OctaGel Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CD89 was purified from the cell supernatant using Ni-NTA affinity resin (Qiagen). Superose 6 SEC was used to further purify CD89 ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... the soluble extracts were subject to Ni-NTA agarose resin (QIAGEN cat#30210). After passing 20 volumes of washing buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Clarified lysate was loaded to a column prepared with Ni-NTA resin (Qiagen). Resin was washed with lysis buffer containing 60 mm imidazole and then protein was eluted with 400 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... His-Hcp was purified from the soluble fraction by NTA-resin chromatography (Qiagen). Purified protein was desalted with a PD-10 desalting column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was then bound to an agarose Ni-NTA resin (Qiagen) and incubated for at least 2 hours at 4 °C followed by extensive washes with 25 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was mixed with Ni-NTA Superflow resin (QIAGEN, Hilden, Germany) and stirred at 4°C for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... TEV was purified via the batch method using Ni-NTA Superflow resin (Qiagen), as described by the manufacturer ...
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bioRxiv - Bioengineering 2023Quote: ... was purified using a Ni-NTA agarose resin column (Qiagen, Venlo, Limburg, Netherlands) and a chitin resin column (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... AbLecs were purified by manual gravity column using nickel-NTA agarose resin (Qiagen). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was incubated with Ni-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, Germany) on a rocker for 1 hour at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell lysates were mixed with 2.5 mL Ni-NTA Agarose resin (Qiagen). Binding to the resin was allowed to proceed for 2 hours while shaking at 110 rpm at 10°C ...
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bioRxiv - Biochemistry 2024Quote: His-tagged proteins were bound to 8 mL of Ni-NTA resin (Qiagen) using gravity flow ...
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bioRxiv - Biophysics 2024Quote: ... The resulting supernatant was incubated with 1.0 mL Ni-NTA Superflow resin (Qiagen) at 4 °C for 2 hours with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... The first step was carried out by using Ni-NTA affinity resin (Qiagen), then pooled fractions ...
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bioRxiv - Biochemistry 2024Quote: ... Cleared lysate was loaded onto a gravity column containing Ni-NTA resin (Qiagen) and washed with 15 CV of LgBiT Lysis Buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Cleared lysate was loaded onto a gravity column with Ni-NTA resin (Qiagen) and washed with 5 CV of SumoTMD Wash Buffer 1 (50 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was incubated with 1.5 mL of Ni2+ NTA resin (Qiagen) for 1 h at 4 °C with rotation ...
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bioRxiv - Systems Biology 2021Quote: ... The supernatant was loaded on a nickel nitrilotriacetic acid (Ni-NTA) resin (Qiagen GmbH) column ...
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bioRxiv - Biochemistry 2020Quote: ... The cell free supernatant was loaded on to pre-equilibrated Ni2+-nitrilotriacetic resin (Qiagen), and incubated for 1hr at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... The soluble hEKL proteins were purified using a Ni-NTA agarose resin (Qiagen, Germany) and subsequently HiLoad 16/60 superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... The soluble protein fraction was then purified with pre-equilibrated Ni-NTA resin (Qiagen), recovered in elution buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: The supernatant was mixed with 2 mL Ni-NTA resin (Ni-NTA agarose; Qiagen) pre-equilibrated with buffer A200 (50 mM Tris pH 7.6 ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were passed over 5 ml of packed Ni-NTA agarose resin (Qiagen) at 1.0 ml/min ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: ... and nickel-nitrilotriacetic acid (Ni-NTA) agarose resin was purchased from Qiagen (Hilden, Germany). PCR reagents ...
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bioRxiv - Cell Biology 2021Quote: ... separated by agarose gel electrophoresis and purified using QiaEx II gel extraction resin (Qiagen). Log-phase promastigotes were transfected with 1-5 μg and cloned by limiting dilution ...
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bioRxiv - Cell Biology 2021Quote: ... before adding it to 2 ml of Strep-Tactin superflow resin (Qiagen, Hilden, Germany) which was pre-equilibrated with 10 ml lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Soluble material was then were added to NTA nickel resin (400 µL slurry, Qiagen), which was pre-charged (overnight at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D532 was purified using 1 mL Ni-NTA agarose resin (Qiagen) according to the manufacturer’s instructions and as described previously [27] with slight modification ...
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bioRxiv - Molecular Biology 2022Quote: ... and the clarified lysate was incubated in batch with Ni-NTA agarose resin (Qiagen) for 1 hour at 4°C with rotation ...
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was supplemented with 1 mL pre-equilibrated Ni-NTA resin (Qiagen) and 10 mM imidazole and stirred for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was applied to a fritted column containing Ni-NTA resin (Qiagen, USA) which had been pre-equilibrated with several column volumes of lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... The resulting supernatant was supplemented with 0.75 mL pre-equilibrated Ni-NTA resin (Qiagen) and 20 mM imidazole and stirred for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate supernatant was passed through a Ni+2-NTA resin column (Qiagen), and protein was purified following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... supernatant was collected and Factor Xa was removed with Factor Xa Removal Resin (QIAGEN). Endotoxin was removed using endotoxin removal resin and Atsttrin purity was determined by SDS-PAGE and reverse phase high performance liquid chromatography (HPLC).
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was added to 0.5 mL (bed volume) Ni-NTA resin (Qiagen) equilibrated in wash/lysis buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Uncleaved protein with 6xHisTag were isolated from the lysate using Ni-NTA resin (Qiagen). The flow-through (FT ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant of the cell lysate was loaded onto Ni-NTA agarose resin (Qiagen) in Poly-prep chromatography columns (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant MBP-Dnmt5(345-747)-6xHis was purified using Ni-NTA agarose resin (Qiagen) and measured by A280 (ε = 139,790 cm-1 M-1).
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bioRxiv - Microbiology 2020Quote: ... the media was collected and applied to 3 ml of Ni-NTA resin (Qiagen). The resin was collected and washed with PBS supplemented with 20 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... These pooled fractions were applied to 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer B with 10 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The first part was loaded onto 200 ul of Ni-NTA Superflow resin (Qiagen) in a gravity column equilibrated with Ni wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was run by gravity over a column of 6ml NiNTA affinity resin (Qiagen) pre-equilibrated in binding buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified by affinity chromatography using a Ni-NTA agarose resin (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... the lysates were loaded onto a gravity-flow column containing Ni–NTA resin (Qiagen) equilibrated in lysis buffer (without PMSF) ...
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bioRxiv - Molecular Biology 2023Quote: ... After purification of AtH2B.9 using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAGEN), the His6-tag portion was removed by TEV protease (30 µg/mg of His6-H2B.9) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The supernatant was then mixed with 0.5 mL of Ni-NTA Agarose resin (Qiagen), equilibrated with 50 mM NaH2PO4 ...
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bioRxiv - Biophysics 2022Quote: ... The remaining supernatant was incubated with 20mM imidazole and 15ml Ni-NTA resin (Qiagen) for 3h with mild agitation ...
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bioRxiv - Biophysics 2022Quote: ... supernatant was combined with Ni-NTA resin (1 mL/1 L of biomass, Qiagen) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... Soluble protein fractions were then mixed with pre-equilibrated Ni-NTA agarose resin (Qiagen) and incubated at 4°C for 1 h with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins harboring the His6 tag were captured by incubation with Ni- NTA resin (Qiagen) (2ml resin per bacterial lysate from 1L culture ...