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Citations for Qiagen :
351 - 400 of 2825 citations for 1 Butylpyrrolidin 1 ium 3 yl 2 cyclopentyl 2 hydroxy 2 phenylacetatechloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The cleared lysate was run through Ni+2-NTA agarose beads (Qiagen [QA], 30250) to allow binding of the histidine-tagged (recombinant ...
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bioRxiv - Cell Biology 2021Quote: ... before adding it to 2 ml of Strep-Tactin superflow resin (Qiagen, Hilden, Germany) which was pre-equilibrated with 10 ml lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... and homogenized for 2 cycles with a Bead Beater TissueLyser II (Qiagen, Germantown, MD) at 24 Hz for 3 min each ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM 2-mercaptoethanol) and the proteins eluted from nickel-NTA agarose beads (Qiagen; using buffer containing 25 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Microbiology 2022Quote: RNA was isolated from 2 x 106 cells using RNeasy plus mini Kit (Qiagen). Reverse transcription was performed with either random decamers or HIV antisense-specific primers ...
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bioRxiv - Microbiology 2020Quote: ... Lung samples were homogenized with DMEM + 2% FBS in a TissueLyser (Qiagen Inc., CA) operated at 25-30 Hz for four minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate supernatant was passed through a Ni+2-NTA resin column (Qiagen), and protein was purified following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Feces samples were homogenized for 2 min at 25 Hz in a TissueLyzerII (Qiagen) using metal beads ...
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bioRxiv - Cell Biology 2020Quote: ... CLEC-2-expressing or PDPN KO FRCs were transfected using Attractene Transfection Reagent (Qiagen) with one or both of the following plasmids ...
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bioRxiv - Immunology 2021Quote: ... The product was run on a 2% gel and purified by gel extraction (Qiagen). Purified product was amplified using primers index3 and index6 ...
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bioRxiv - Immunology 2021Quote: ... The product was run on a 2% gel and purified by gel extraction (Qiagen). Purified product was amplified using primers index3 and index4 ...
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bioRxiv - Immunology 2021Quote: ... PCR products were run on 2% agarose gels and purified by gel extraction (Qiagen). Purified barcode and gene products were combined with linearized yeast-display vector (pDD003 digested with EcoRI and BamHI ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RNA was detected using One-step probe RT-qPCR kits (Qiagen) run on the CFX96 detection system (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RNA was detected using One-step probe RT-qPCR kits (Qiagen) run on the CFX96 detection system (Bio-Rad) ...
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bioRxiv - Genetics 2020Quote: ... 2 µL of bisulfite-converted DNA were amplified with the HotStartTaq DNA Polymerase (QIAGEN) and primers containing internal barcodes using following conditions ...
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bioRxiv - Pathology 2023Quote: ... with 2 x 3.2 mm stainless steel beads using a TissueLyser (Qiagen, Germantown, MD) for 3 minutes at 30 r/s for 3 cycles ...
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bioRxiv - Pathology 2023Quote: ... with 2 x 5 mm stainless steel beads using a TissueLyser (Qiagen, Germantown, MD) for 3 minutes at 25 r/s for 2 cycles ...
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bioRxiv - Microbiology 2023Quote: ... collected in DMEM with 2% serum and homogenized using a Tissue-Lyser II (QIAGEN). Samples were centrifuged at 8,000 rpm for 8 minutes and viral titers were quantified using a plaque assay as described above ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenization was performed with 2 tungsten beads using a Tissue Lyser (Qiagen, cat# 85300) run at 30 cycles/sec ...
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bioRxiv - Pathology 2022Quote: ... with 2 x 5 mm stainless steel beads using a TissueLyser (Qiagen, Germantown, MD) for 3 minutes at 25 r/s for 2 cycles ...
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bioRxiv - Developmental Biology 2023Quote: 2 μg of genomic DNA was bisulfite treated using the EpiTect Bisulfite Kit (Qiagen) and eluted in 20 μL of 1:10 of the supplied EB buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The FLAG elution was transferred to 2 ml of NI-NTA agarose resin (Qiagen) and incubated for 1 hour ...
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bioRxiv - Genomics 2024Quote: ... The product was purified from a 2% agarose gel (QIAquick Gel Extraction Kit, Qiagen). In parallel ...
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bioRxiv - Molecular Biology 2024Quote: ... until saturated then immersed in 2 ml RNAprotect Cell Reagent or RNALater (Qiagen Corporation) or collected on GenTegra Ahlstrom GenSaverCards for Blood and Saliva (Gentegra Corporation) ...
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bioRxiv - Neuroscience 2022Quote: ... Mice received either miRCURY LNA power inhibitor for mmu-miR-335-5p (Ant-335; sequence: 5’-CATTTTTCGTTATTGCTCTTG-3’; Qiagen, Cat No.: 339132; 0.1 nmol in 2 μL PBS), or a non-targeting scrambled control (Scr ...
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bioRxiv - Cancer Biology 2024Quote: ... was achieved from frozen tissue biopsies upon homogenization with stainless steel beads (3–7 mm mean diameter) using TissueLyser II (QIAGEN; 20” at 30 Hz, for 2 cycles(20)) ...
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bioRxiv - Cancer Biology 2021Quote: ... After 2 hours we extracted the total RNA (RNeasy mini kit, Qiagen, cat. n. 74104) from 40 animals for each of the 3 experimental replicates (total ...
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bioRxiv - Cell Biology 2020Quote: RNA from 2×107 cells was isolated using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands), cDNA was generated by reverse transcription on CFX96 real-time system (BioRad ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 2 μl sucrose purified virus using the RNeasy mini kit (QIAgen). The RNA was then treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by 2 minutes (40 oscillation per second) on a bead shaker (Qiagen Tissue lyser)-centrifuged at 1000g for 5 minutes at 4 °C to remove debris ...
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bioRxiv - Molecular Biology 2020Quote: ... and gonad were collected and stored separately in 2-mL tubes containing RNA later (QIAGEN) overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... coli* culture from each growth condition were stabilised in RNAProtect Bacteria Reagent (2 mL; Qiagen) and cells pellets were frozen at −80°C prior to processing ...
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bioRxiv - Developmental Biology 2020Quote: ... for 2 hours after which samples were treated with 5 μL Proteinase K (Qiagen 19131) overnight ...
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bioRxiv - Bioengineering 2021Quote: ... the obtained soluble extract was mixed with 2 ml slurry Ni-NTA beads (30210, QIAGEN), incubated at 4 °C for 2 h with rotation.
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bioRxiv - Biochemistry 2021Quote: ... the obtained soluble extract was mixed with 2 ml slurry Ni-NTA beads (30210, QIAGEN), incubated at 4°C for 2 h with rotation ...
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bioRxiv - Microbiology 2020Quote: RNA was isolated from U-2 OS cells with the RNeasy minikit (Qiagen, Hilden, Germany). 0.5µg RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 μl of bisDNA was added to 2 μl 10x PCR buffer (Qiagen, Cat# 203203), 2 μl of 10 μM primer mix (Methods Table 1) ...
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AMPK is elevated in human cachectic muscle and prevents cancer-induced metabolic dysfunction in micebioRxiv - Molecular Biology 2022Quote: ... the muscles were homogenized (2 × 30 sec, 30 Hz, TissueLyser II bead mill, Qiagen, USA) in a modified GSK3-buffer (10% glycerol ...
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bioRxiv - Genetics 2022Quote: ... RNA was collected from 2 x 105 pelleted cells using the RNeasy mini kit (Qiagen). qPCR using primers specific for ERAP1 or TNFSF12 were used to validate knockdown relative to 18S rRNA.
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bioRxiv - Biophysics 2022Quote: ... the supernatant was incubated for 2 h at 4 °C with Ni2+-NTA-agarose (Qiagen) (20 mg of proteins/ml of resin ...
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bioRxiv - Microbiology 2021Quote: ... The samples were then homogenized using a Vortex-Genie 2 and vortex adapter (QIAGEN®) at the highest setting for 10 minutes.
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bioRxiv - Genomics 2022Quote: ... Lysate was spun down and supernatant was mixed with 2 ml Ni-NTA resin (Qiagen) per 50 ml sample and incubated for 1h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... BEAS-2B and MRC-5 (2×106 cells/well) using the RNeasy Mini Kit (Qiagen) with DNase I treatment to eliminate DNA contaminants as previously described18 ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was quantified by RT-qPCR with the QuantiTect Kit (Qiagen, 204443) with Sarbeco E gene primers and probe in a QuantStudio 3 thermocycler (Applied Biosystems) ...
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bioRxiv - Systems Biology 2020Quote: RNA was isolated from 2 x 106 cells with the RNeasy Plus Mini kit (Qiagen) and reversely transcribed with SuperScript III (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... and collected into a PCR tube containing 7 µL of 2×TCL lysis buffer (Qiagen) with 2% v/v 2-mercaptoethanol (Sigma) ...
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bioRxiv - Genetics 2021Quote: ... Other ventriculus sinister sections were pestled by a grinding apparatus (Schwingmühle TissueLyser 2, Qiagen, Germany) and stored at −80°C for detection ...
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bioRxiv - Immunology 2021Quote: ... Skin samples were homogenized in a TissueLyser LT (Qiagen, 50 Hz, 2 times 4 minutes) using 5 mm stainless steel beads (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR primers were designed using the PyroMark Assay Design 2.0 software (Qiagen, Supplementary Table 2). The assay for MBP was tested for its sensitivity using the EpiTect PCR Control DNA Set (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... siRNA-transfection reagent complexes were generated using 2 μL HiPerfect transfection reagent (Qiagen, Germantown, MD) and 25-50 nM siRNA (non-specific control siRNA (sc-37007 ...