Labshake search
Citations for Qiagen :
3851 - 3900 of 10000+ citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... DNA extraction from the sediment samples was performed with a commercially available extraction kit (DNAeasy PowerSoil Kit©, QIAGEN) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... or postmortem human cerebellar vermis samples using either the RNeasy Kit or RNeasy Fibrous Tissue Kit following the manufacturer’s protocols (QIAGEN). For tissue RNA extraction ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed using a Qiagen kit with the EZ1 DNA Tissue Kit following the manufacturer’s instructions (Qiagen) and DNA was eluted in a 50-μL volume [5].
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from cell pellets using a genomic DNA isolation kit (Blood & Cell Culture Midi kit (Qiagen)) ...
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bioRxiv - Immunology 2024Quote: RNA was extracted from blood or biopsies following manufacturer’s protocol using the PAXgene kit and Qiagen Rneasy Mini kit (Qiagen), respectively ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from primary mouse hepatocytes and Huh-7 cells using the RNeasy Plus Micro Kit and cDNA synthesis performed using the QuantiTect Reverse Transcription Kit according to the manufacturer’s protocol (Qiagen). Reactions were performed in triplicate in 384-well plate format ...
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CD4+ T cells regulate sickness-induced anorexia and fat wasting during a chronic parasitic infectionbioRxiv - Immunology 2022Quote: ... DNA from the blood or tissue of mice was extracted using the same kit (Qiagen blood and tissue kit) and qPCR was done using the same primers ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA removed and RNA extracted using a commercial kit (RNeasy Plus Mini Kit, Cat. 74134, Qiagen, Hilden, Germany). Sample processing for quality control and sequencing was completed by the Australian Genome Research Facility (Victoria ...
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bioRxiv - Bioengineering 2023Quote: Total RNA and cDNA were synthesized from the samples using RNeasy mini kit and QuantiTect Reverse Transcription Kit according to the manufacturer’s instructions (Qiagen). qPCR was performed in a Biorad Thermocycler with a reaction volume of 20 µl which constitutes primers (0.5 µM) ...
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bioRxiv - Cancer Biology 2023Quote: ... was isolated using Midi or Maxi kits for the validation screens gDNA was isolated using Midi kits according to the manufacturer’s protocol (Qiagen). PCR and sequencing were performed as previously described.81 ...
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bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
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bioRxiv - Microbiology 2023Quote: ... Three nucleic acid extraction kits designed for DNA and RNA extraction were compared: DNeasy PowerWater kit (QIAGEN; Hilden, Germany), NucleoSpin Soil ...
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bioRxiv - Microbiology 2024Quote: ... and RNA was extracted following the manufacturers instructions (Zymo Quick-RNATM Viral Kit or QIAGEN RNeasy Mini Kit, respectively).
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bioRxiv - Biophysics 2021Quote: ... The capture probes contained an 11- to 12-nt target-capturing sequence with 4 LNA residues (Tm = ~70°C, estimated using Qiagen web application) for high affinity and kinetically stable capturing of nucleic acid targets on the surface ...
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bioRxiv - Genomics 2020Quote: ... RNA from in vitro conidia and hyphae harvested 3 and 7 days post-inoculation of potato dextrose broth grown in the dark at 24°C were extracted with RNeasy spin columns (QIAGEN, Venlo, Netherlands). Illumina TruSeq RNAseq libraries were prepared according to the manufacturer’s instructions and sequenced on an Illumina HiSeq 2500 sequencer (50 bp single reads) ...
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bioRxiv - Plant Biology 2020Quote: ... Plant material was dried at 60°C overnight and ground to powder using a ball mill (Tissue Lyser II, Qiagen, Venlo, Netherlands). Aliquots of about 1.5-2 mg were transferred into tin capsules (IVA Analysentechnik ...
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bioRxiv - Plant Biology 2022Quote: ... Tissue was ground to a fine powder at −80°C using 3-mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA). Ground samples were extracted with 1 mL of cold methanol containing [phenyl 13C6]-IAA (0.1 nmol/mL ...
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bioRxiv - Neuroscience 2021Quote: ... spun down (400 g × 10’ at 4 °C) and the pellet was resuspended in 600μ of Qiazol Lysis Reagent (Qiagen, Cat.No. 79306) and kept at −80 °C before sequencing.
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bioRxiv - Microbiology 2019Quote: ... and then amplified using 40 cycles of 95°C for 3 s and 60°C for 30 s using either the Rotor-Gene Q or Rotor-Gene 6000 platform (Qiagen, Hilden, Germany).
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bioRxiv - Biochemistry 2020Quote: ... at 4°C and the supernatant was loaded onto a 1 ml nickel nitrilotriacetic acid-agarose column (Ni-NTA, Qiagen, Hilden, Germany) previously equilibrated with the corresponding lysis buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... step for 15 min at 4°C to remove insolubilized material and the supernatant was incubated with Ni-NTA agarose beads (Qiagen, cat: 30230) for 2 hrs ...
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bioRxiv - Evolutionary Biology 2021Quote: Whole bodies of individual male flies were removed from the -80°C freezer and immediately homogenized in Qiazol lysis reagent (Qiagen, Hilden, Germany). The homogenate was treated with DNase I followed by addition of chloroform ...
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bioRxiv - Zoology 2021Quote: ... dead mites were pierced with an 18-gauge needle under a dissecting microscope and digested overnight in lysis buffer and proteinase K at 56 °C (Qiagen, Valencia, CA). Final DNA product from each mite was eluted in 60 µL of AE buffer ...
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bioRxiv - Immunology 2021Quote: ... cells were spun down and the supernatant was removed to either store the cells at -80° C before proceeding with the analyses either as a pellet or to resuspend in RLT (Qiagen, cat 79216) + 1% 2-mercaptoethanol ...
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bioRxiv - Plant Biology 2022Quote: ... Tissue was ground to a fine powder at −80°C using 3-mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA). Ground samples were extracted with 1 mL of cold methanol containing [phenyl 13C6]-IAA (0.1 nmol/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were weighed and homogenized in 4 volumes of water (4 µL of water/mg tissue, 4°C) using a bead beater (TissueLyser II, QIAGEN; Germantown, MD). Aqueous homogenates were profiled using four complimentary liquid chromatography tandem mass spectrometry (LC-MS ...
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bioRxiv - Cancer Biology 2024Quote: ... Targeted knockdown was conducted with 100nM ON-TARGETplus smartpool human c-MYC (mixture of 4 individual siRNA) and/or human KLF6-SV1 siRNA (Dharmacon, Lafayette, CO) with HiPerfect (Qiagen, Valencia, CA).
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bioRxiv - Bioengineering 2024Quote: Whole mouse aortas (n=18 per group) cleaned of perivascular tissue were perfused with sterile HBSS and stabilized overnight at 4◦C in RNAProtect reagent (Qiagen; Hilden, Germany) before long-term storage at -80◦C ...
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bioRxiv - Cell Biology 2023Quote: ... collected into an Eppendorf tube and centrifuged at 11200 rpm at 4 °C for 6 min followed by pellet resuspension with 200 μl of QIAzol (Qiagen, Hilden, Germany). To isolate EC from the bottom layer ...
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bioRxiv - Cancer Biology 2023Quote: ... 45 cycles of denaturation at 95 °C for 15 s and annealing at 60 °C for 1 min following a melting step in a Rotor-Gene Q machine (Qiagen, Hilden, Germany). GAPDH was used for normalization ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 20,000 x g for 45 minutes at 4°C and incubated with Ni-NTA agarose resin (Qiagen, catalogue number: 30230) pre-equilibrated with lysis buffer for 2 hours at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... Collected tissue was ground to a fine powder at -80°C using 3 mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA), and between 10-15 mg of ground tissue per sample was used for auxin extraction ...
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bioRxiv - Immunology 2021Quote: ... and probe (375 nM, 5’-6FAM-ACACTAGCC/ZEN/ATCCTTACTGCGCTTCG-IABkFQ-3’) with 12.5μL of 2X QuantiFast Probe Mix (QIAGEN), 0.25μL of 2X QuantiFast RT Mix (QIAGEN) ...
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bioRxiv - Cell Biology 2019Quote: ... and SHARPIN siRNA (5’-GCUAGUAAUUAAAGACACAd(TT)-3’) and the scramble Allstars negative control siRNA were ordered from QIAGEN. Gene silencing was performed using siRNA oligonucleotides and Lipofectamine RNAiMax reagent (13778150 ...
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bioRxiv - Microbiology 2021Quote: ... 20 mM imidazole was added to the supernatant and incubated with 5 mL of Ni-NTA resin (Qiagen) with a stirring magnet at 4°C for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... we added 0.6 μL Proteinase K stop solution to each tube (5 mg/mL Proteinase K solution (Qiagen), 50 mM EDTA ...
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bioRxiv - Developmental Biology 2019Quote: ... The products from 3 to 5 PCR reactions were pooled before purifying the DNA on MinElute columns (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... prior to the addition of proteinase K and 5 μl of 100 mg ml−1 RNase A (Qiagen). The DNA concentration was determined using a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Red blood cells were removed by treating the cells with 5 ml Red Blood Cell Lysis buffer (Qiagen) for 4 min ...
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Alzheimer’s patient brain myeloid cells exhibit enhanced aging and unique transcriptional activationbioRxiv - Neuroscience 2019Quote: ... FACS-isolated cell populations were spun at 5,000 rcf for 5 minutes and resuspended in 0.35 ml Buffer RLT from Qiagen RNeasy Micro kit ...
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bioRxiv - Cancer Biology 2019Quote: ... The reverse transcription step exactly followed SMART-seq2 protocol with 5’-biotinylated TSO (Qiagen, primers see Table S9). The first round of 24-cycle PCR started with 10µl reverse transcription product ...
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bioRxiv - Developmental Biology 2022Quote: ... Complementary DNA of microRNAs was synthesized from 5 μg total RNA using microRNA-specific primers (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... A 5 ml aliquot of culture was removed and added to 10 ml of RNAprotect Bacteria Reagent (Qiagen) and mixed by vortexing ...
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bioRxiv - Cell Biology 2023Quote: ... For experiments with HURP knockdown the custom siRNA with 5’ to 3’ sequence used was AGUUACACCUGGACUCCUUTT (Qiagen, 1027423).
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bioRxiv - Microbiology 2023Quote: ... and homogenised with 3-5 mm steel beads in a TissueLyser II (Qiagen, 24 Hz, 2 × 3 min). 350 μL lysed blood or 200 μL lysed head kidney were transferred to a Magna Pure 96 Processing Cartridge (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Edmund Bühler) by bead beating twice for 5 min at 30 Hz in a Tissue Lyzer II (QIAGEN). Lysates were then incubated for 30 min at 37 °C with shaking ...
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bioRxiv - Physiology 2023Quote: ... a 5 mg piece of kidney tissue was homogenized in 350 μL of RNeasy RLT Lysis buffer (Qiagen) containing 2-mercaptoethanol and centrifuged at maximum speed for 3 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Sequences with an E-value lower than 1e-5 were used for multiple sequence alignment using CLC v 21.0.5 sequence manager (Qiagen). After multiple rounds of alignments and manual removing non-nAChR sequences a set of 2047 proteins were obtained ...
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bioRxiv - Biochemistry 2023Quote: ... High speed supernatant (HSS) was then batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin at 4ºC for 2 hours stirring in a beaker ...
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bioRxiv - Neuroscience 2023Quote: ... LNA-CTG and the control scrambled LNA-modified sequence (LNA-SCB) 5′-GTGTAACACGTCTATACGCCCA-3′ were obtained from Qiagen as previously described in Rue et al 34