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Citations for Qiagen :
3801 - 3850 of 4028 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Mosquito bodies were homogenized in PBS supplemented with 20% FBS and 2% penicillin/streptomycin with 5mm stainless steel beads with a TissueLyser (Qiagen) prior to RNA isolation ...
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bioRxiv - Microbiology 2020Quote: 3-5 × 105 cells with or without treatment with IFNα and SARS-COV-2 infection were harvested and total RNA was extracted using the RNeasy kit (Qiagen) employing on-column DNase treatment ...
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bioRxiv - Microbiology 2020Quote: ... buffer with lysozyme (1mg/mL) and incubated at RT for 2 min prior to adding 700 μL RLT Buffer (Qiagen) with 10ul/mL B-mercaptoethanol (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from the testis and striatum of three 2 months old mice using the RNeasy Mini Kit (Qiagen) and was used for random-primed cDNA synthesis with SuperScript III reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA concentration and purity were measured by a spectrophotometer and 2 μg were used to synthesize cDNA by QuantiTect Reverse Transcription kit (Qiagen). Real-time amplification was performed in a Mastercycler EP Realplex (Eppendorf ...
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bioRxiv - Molecular Biology 2020Quote: ... eluates were supplemented with imidazole at a final concentration of 10mM and incubated for 2 hours with 200 µL of Ni-NTA agarose beads (Qiagen) that were prewashed in buffer C (20mM HEPES [pH 7.6] ...
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bioRxiv - Immunology 2021Quote: ... 150 ng DNA per reaction was amplified in duplicate using primers and probes specific to γHV68 Orf50 and mouse Ptger2 (see table) and 2× QuantiNova Probe Mastermix (Qiagen). Standard curves were obtained by serial dilutions of Orf50 and Ptger2 gBlocks (ORF50 ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 2-week-old leaves by using QIAGEN RNeasy Plant Mini Kit (QIAGEN, Germantown, MD, 74904). Five μg of total RNA was treated with DNase following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Sorted CD14+ monocytes from total PBMC either ex vivo or after a 20 hour stimulation with 106 UV-inactivated SARS-CoV-2 viral particles per 106 cells were lysed with RLT Plus buffer (QIAGEN). RNA was isolated using the RNeasy Micro Plus Kit (QIAGEN ...
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bioRxiv - Microbiology 2019Quote: ... Amplicons displaying bands at ~250 bp on a 2% agarose gel were purified using the QIAquick PCR purification kit (Qiagen). Purified samples were quantified with PicoGreen (Invitrogen ...
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bioRxiv - Genetics 2019Quote: ... The 0.45 μm cellulose filters were immediately placed in 2 mL tubes with 1440 μL ATL-buffer (Qiagen, Hilden, Germany), whereas the 2.0 μm glassfiber filters were placed in 5 mL tubes with 4050 μL ATL-buffer ...
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bioRxiv - Physiology 2019Quote: Total RNA was extracted from cell pellets or 20mg of tissue using Tri-Reagent and chloroform and homogenisation using a tissue lyser for 2 minutes at 20Hz (Qiagen). RNA was extracted using RNeasy Mini kit (Qiagen ...
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bioRxiv - Genetics 2019Quote: ... Ligation products were size-selected (250-300 bp) and purified from a 2% low-melting agarose gel using the MinElute Gel Extraction Kit (Qiagen). Ampure XP beads (Agilent ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatant was applied twice to a column containing a gel bed of 2 ml nickel-nitriloacetic acid agarose (Qiagen, www.quiagen.com) equilibrated with lysis buffer ...
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bioRxiv - Genomics 2020Quote: ... The pooled sample was quantified using the KAPA Library Quantification Kit and adjusted to 2-4nM with Buffer EB (QIAGEN) for sequencing on Illumina platforms ...
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bioRxiv - Molecular Biology 2020Quote: ... Genomic DNA was extracted from samples of 2×109 cells after lysis using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The effect of IscR-C92A on σ70-dependent activity was determined by incubating IscR-C92A with 2 nM supercoiled pPK12778 [purified with the QIAfilter Maxi kit (Qiagen)] ...
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bioRxiv - Microbiology 2019Quote: ... sorted cells were incubated for 2 h at 55 °C and genomic DNA was extracted using Blood and Tissue DNA extraction kit (Qiagen), by omitting the lysis step ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: 3-5 x 105 cells infected or not with SARS-CoV-2 or HCoV-NL63 were harvested and total RNA was extracted using the RNeasy kit (Qiagen) employing on-column DNase treatment ...
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bioRxiv - Zoology 2020Quote: ... 8 μL of the amplified product were electrophoresed in an ethidium bromide stained 2% gel The amplicons obtained were isolated and purified using a QIAquick pcr purification kit (Qiagen), and the purified products were sequenced by Eurofins ...
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bioRxiv - Cell Biology 2020Quote: ... and gene expression was assessed using RT2 Profiler PCR Array Human WNT Signaling Pathway Plus (PAHS-043YC-2, Qiagen Germany). Arrays were run on QuantStudio6 (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: Mouse muscles were pulverized in liquid nitrogen and homogenized 2 × 0.5 min at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in ice-cold homogenization buffer (10% glycerol ...
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bioRxiv - Plant Biology 2019Quote: ... fifty milligrams of fresh leaves in a 2-ml tube was frozen in liquid nitrogen and ground using a Tissuelyser II (Qiagen). The ground sample was extracted with 500 μl of extraction solvent ...
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bioRxiv - Physiology 2019Quote: Muscles were pulverized in liquid nitrogen and homogenized 2 × 0.5 min at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in ice-cold homogenization buffer (10% glycerol ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was separated on a 2% agarose gel and bands between 120-150 bp were excised and purified (QIAquick Gel Extraction Kit, Qiagen). Chromatin accessibility was assayed using quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... each of forward and reverse primers and 2 μl of nuclease-free water) was run in a Rotor-Gene Q thermal cycler (Qiagen) using 40 cycles of 95°C for 10 seconds ...
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bioRxiv - Genetics 2020Quote: U8 DNA templates containing a T7 consensus sequence were PCR amplified from human or zebrafish genomic DNA (see Supplementary Table 2 for primer sequences) and purified after agarose gel electrophoresis using a QIAEX II kit (Qiagen). Human and zebrafish U8 snoRNAs were generated using 400-1000ng of template DNA and a mMESSAGE mMACHINE T7 kit (Life Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we designed strain-specific primers (see Supplementary Table 2) and generated overlapping amplicons using the OneStep RT-PCR Kit (Qiagen) and 5 µL of cDNA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We ran the library in three lanes of a 2% agarose gel and extracted the 300-400 bp fragments using a QIAquick Gel Extraction Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble extracts were applied to 2-ml columns of nickel-nitrilotriacetic acid- agarose (Ni-NTA) (QIAGEN catalog no. 30210) that had been equilibrated with lysis buffer without protease inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... Ten-fold serial dilution of the SARS-CoV-2 stock were prepared using PBS and viral genomic RNA extracted using the QIAamp viral RNA mini extraction kit (Qiagen) according to the manufacturers spin protocol and the recommended 5.6 μg polyA carrier RNA/140 μL virus sample ...
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bioRxiv - Biochemistry 2021Quote: ... and 675 μL PCR products for control cells and 320 μL were applied to the 2% agarose gel for purification using QIAquick Gel Extraction Kit (QIAGEN). The PCR products of control and sort2 were concentrated by using Amicon Ultra-0.5 10K (UFC501096 ...
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bioRxiv - Neuroscience 2021Quote: Fluorophore TYE665 labeled locked nuclear acid (LNA) (C4G2)6 and (CCG)8 probes (Supplemental table 2) were synthesized by Qiagen. The FISH protocol was adapted from (DeJesus-Hernandez et al ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells seeded into 100 mm dishes were transfected at a confluence of 60 % with 2 µg of cDNA encoding PTH1RFRET with Lipofectamine 3000 Transfection Reagent Kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... 2 ml of peripheral blood was used for germline DNA extraction using the QIAamp DNA Blood midi kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The final library sample was purified from a 2% agarose gel with silica beads (QIAEX II Gel Extraction Kit, Qiagen). 125bp paired-end sequencing was run on an Illumina HiSeq2500 sequencer at the CRG Genomics Core Facility.
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells in 6-well plates were transfected with 2 μg pIRES2-eGFP or Trim-HA-NUP153 derivatives using Effectene (Qiagen). At 24 h post-transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA concentration and purity were measured by a spectrophotometer and 2 ug were used to synthesize cDNA by QuantiTect Reverse Transcription kit (Qiagen). Real-time amplification was performed in a Mastercycler EP Realplex (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: ... Mosquito bodies and legs and wings were put into a 2 ml round-bottom tubes containing 250 ml of PBS and a steel ball (Qiagen). Samples were homogenized using a TissueLyser (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... in Tris/HCl/Ca+2 buffer was subjected to extraction in house using the silica method or QIAamp viral RNA mini kit (Qiagen) according to the manufacturer’s guidelines.
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bioRxiv - Molecular Biology 2021Quote: ... The specimens were gently and quickly homogenized using a BioMasher II homogenizer (Funakoshi) and mixed with 2 mL of Buffer G2 (QIAGEN), including 200 µg/mL RNase A and 50 µL proteinase K (20 mg/mL) ...
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bioRxiv - Immunology 2021Quote: ... Amplification was confirmed by running samples on a 2% agarose gel prior to PCR Clean up (QiaQuick PCR Purification kit, Qiagen). Samples were Sanger sequenced (Source Bioscience ...
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bioRxiv - Immunology 2021Quote: Viral RNA was isolated from rhesus macaque plasma from 2 weeks post-SIV infection using an Ultrasense Viral RNA kit (QIAGEN) and cDNA was reverse transcribed using the Applied Biosystems High Capacity cDNA synthesis kit (Thermo-Fisher) ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed with a primer set targeting partial regions of the ORF1b gene in the SARS-CoV-2 virus using the QIAGEN OneStep RT-PCR kit (Qiagen) as previously reported (51) ...
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bioRxiv - Genetics 2021Quote: ... frozen and lyophilised in a 2 ml 96 deep-well plate following which the samples were ground in the TissueLyser II (QIAGEN) using a steel ball in each well for 4-6 minutes at a frequency of 25 Hz ...
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bioRxiv - Genetics 2019Quote: ... in each well of 8-stripped PCR tubes by shaking with a zirconia bead (2 mm in diameter, Nikkato, Japan) in TissuLyser II (Qiagen) for 30 s at 30 Hz ...