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Citations for Qiagen :
301 - 350 of 5853 citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and Ant-has-miR-130b-5p (Qiagen, MIMAT0004680) were employed to inhibit the respective microRNAs ...
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bioRxiv - Molecular Biology 2023Quote: ... Mimics of mmu-miR-130b-3p (Qiagen, MSY0000387), mmu-miR-130b-5p (Qiagen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... from the wild population by combining each forward primer with a reverse primer designed within the adjacent amplicon (Supplementary Table 6) and using the LongRange PCR kit (QIAGEN). When the primer sets originally designed from zebra finch sequences failed to produce long amplicons ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA generated after performing reverse transcription of RNA product was subjected to quantitative PCR (qPCR) analysis using gene-specific predesigned primers (RT2 qPCR Primer Assay, Qiagen), listed in Supplementary Table S4 ...
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bioRxiv - Neuroscience 2020Quote: ... Fold enrichment for each primer set was normalized to input and reported relative to a negative control gene desert region (mouse Igx1a (Qiagen). Primers ...
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bioRxiv - Immunology 2020Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cβ primer ...
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bioRxiv - Immunology 2024Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cý primer ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 nM of each primer and 5 µl of SYBR Green QuantiFast RT-qPCR master mix (Qiagen). Primers used were ...
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bioRxiv - Cancer Biology 2022Quote: ... primer array of the RT2 Profiler PCR Array (#PAMM-025ZD, Qiagen, Germany) was used according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... which used PyrG (CTP synthase) primers and HotStarTaq PCR reagents (QIAGEN, #203203). DNA samples were quantified then diluted to 20 ng/µL so that 5 µL could be used in each 25-μL PCR reaction ...
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bioRxiv - Bioengineering 2023Quote: ... and primers from miRCURY LNA miRNA PCR Assays (Catalog No. 339306, Qiagen) for hsa-miR-21-5p (GeneGlobe ID YP00204230) ...
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bioRxiv - Microbiology 2024Quote: ... Expression levels were measured by quantitative PCR using pre-designed primers (Qiagen) and SYBR green (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... and was subsequently subjected to reverse transcription followed by PCR using a OneStep RT-PCR Kit (QIAGEN) with primers for human Top2A ...
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bioRxiv - Microbiology 2021Quote: ... The assay was performed using qRT-PCR and the QuantiNova Probe RT-PCR kit (Qiagen, Düsseldorf, Germany), and the LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Genomics 2019Quote: ... The RT-PCR reactions were performed with a QuantiTect SYBR Green PCR Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: PCR amplification of the RNA was completed using the One-step Syber Green RT-PCR Kit (Qiagen). 25ng of total RNA was added to a master mix reaction of the provided RT Mix ...
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bioRxiv - Microbiology 2022Quote: PCR amplification of the RNA was completed using the One-step Syber Green RT-PCR kit (Qiagen). 25ng of total RNA was added to a master mix reaction of the provided RT mix ...
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bioRxiv - Microbiology 2024Quote: ... rpsL and qoxA transcripts by qRT‒PCR was performed with QuantiFast SYBR Green RT‒PCR Kit (Qiagen) using the Quantstudio3 system (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... BALF and serum was performed by quantitative reverse-transcriptase PCR (qRT-PCR) using the one-step Quantinova™ Probe RT-PCR kit (QIAGEN). Primers and a TaqMan probe were designed to hybridize to a sequence in ORF7 (encoding N) ...
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bioRxiv - Bioengineering 2021Quote: ... One-step reverse transcription quantitative polymerase chain reaction (RT-qPCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) with 7.8 μl purified viral RNA or specified quantities (2.5 ng or 100 ng ...
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bioRxiv - Cell Biology 2020Quote: ... was used to extract RNA and the OneStep RT-PCR Kit (Qiagen) was used to synthesize cDNA ...
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bioRxiv - Genomics 2020Quote: ... while the cDNA amplicons were made with OneStep RT-PCR kit (QIAGEN). Manufacturer protocols were followed for all experiments ...
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bioRxiv - Cell Biology 2019Quote: ... in a single step using the Qiagen OneStep RT-PCR Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Amplifications were done using the OneStep RT-PCR kit (QIAgen, California, USA) with the following conditions ...
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bioRxiv - Immunology 2021Quote: ... to perform RT² Profiler™ PCR Array Mouse Hypoxia Signaling Pathway (Qiagen) real-time PCR in Stratagene MX3000p qPCR system under these cycling conditions ...
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bioRxiv - Plant Biology 2020Quote: ... Amplification was carried out using a One-Step RT-PCR Kit (QIAGEN) and BIO-RAD T100 Thermal Cycler ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR experiments were performed with Rotor-Gene-Q machine (Qiagen) using the 1x Takyon SYBR Master Mix (Eurogentec ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-qPCR was performed using the QuantiFast SYBR Green PCR kit (Qiagen). The PCR reaction was conducted on a Corbett Rotor-Gene 6000 (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... and the S gene was amplified using OneStep RT-PCR Kit (Qiagen). The mutations were identified by Sanger sequencing (GENEWIZ) ...
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bioRxiv - Immunology 2022Quote: ... and the S gene was amplified using OneStep RT-PCR Kit (Qiagen). The mutations were identified by Sanger sequencing (GENEWIZ) ...
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bioRxiv - Microbiology 2022Quote: ... All assays were adapted to the One-step RT-PCR Kit (Qiagen) reaction chemistry ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative RT-PCR was performed in triplicate using SYBR green mix (Qiagen) on an ABI 7500 machine (Applied Bio Systems) ...
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bioRxiv - Genetics 2019Quote: ... RT-qPCR was performed with QuantiTect SYBR Green PCR Kit (QIAGEN, Germany) according to the manufacturer’s instruction by CFX Connect™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: RNA was quantified using the QuantiFast SYBR Green RT-PCR Kit (Qiagen). Primers used were for Esr1 (QuantiTect Primer Assays ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative RT-PCR was performed using the SYBR Green detection system (Qiagen) according to the manufacturer’s instructions in an iCycler (Bio-Rad Laboratories Ltd. ...
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bioRxiv - Immunology 2022Quote: ... and the S gene was amplified using OneStep RT-PCR Kit (Qiagen). The mutations were identified by Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2022Quote: ... then the template was amplified using the OneStep RT-PCR Kit (Qiagen) with the EILV-specific primers 5’-CGA CGA TGA CCG GAG AAG AG -3’ and reverse primer 5’-AAG ACT CGG TCT GCC TGC −3’ ...
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bioRxiv - Neuroscience 2023Quote: ... gcy-8 mRNA was detected using a OneStep RT-PCR kit (QIAGEN) from 3 ng of template RNA ...
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bioRxiv - Physiology 2023Quote: ... RT-qPCR was then performed with QuantiTect SYBR Green PCR Kit (Qiagen) on a LightCycler 480 (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... RT PCR was carried out in a Rotor-gene Q2 PLEX (QIAGEN), using SensiMix SYBR master mix (Bioline) ...
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bioRxiv - Microbiology 2023Quote: ... A nested RT-PCR (QIAGEN hot start Taq master kit 1000 units) was then performed from 2 µL of previous amplicon for 15 min at 95°C then 35 cycles of amplification (30 sec at 94°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The ligated products were amplified by PCR with Illumina primer pairs and purified with PCR purification kit (QIAGEN). 5C libraries were sequenced with the 90 bp pair-end mode by Hi-seq 2500 platform of Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... Inserts were PCR amplified using M13 primers and template plasmid was digested with DpnI before PCR purification (Qiagen). Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega) ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time reverse transcription PCR (qRT-PCR) was carried out with the QuantiNova SYBR Green RT-PCR kit (Qiagen, Manchester USA) using a LightCycler® 480 Instrument II (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... HGF (5’-CTCACACCCGCTGGGAGTAC-3’, 5’-TCCTTGACCTTGGATGCATTC-3’), STAT3 (5’-CTTTGAGACCGAGGTGTATCACC-3’, 5’-GGTCAGCATGTTGTACCACAGG-3’) and B-Actin Primer Set (Qiagen, QT00095431). After preparing master mixes samples were prepared in quadruplicate in a 96-well Reaction Microplates (Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Hsa-miR-124-3p Locked Nucleic Acid (LNA) power inhibitor and has-miR-124- 3p miRCURY LNA miRNA mimic (Qiagen, Germantown, MD) were resuspended with RNase-free water to 100 μM ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time PCR was performed using the Custom RT² Profiler PCR Array according to the manufacturer’s instructions (Qiagen), and detection was performed using the ABI Prism® 7500 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... RNA samples were subjected to KSHV real-time PCR array using the QuantiTect Probe RT-PCR Kit (Qiagen). The copy number of each transcripts was normalized to that of GAPDH transcripts and the ratio of treated versus untreated cells was calculated.
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bioRxiv - Neuroscience 2021Quote: ... cDNA was processed for real time q-PCR using SYBR-green (QuantiFast SYBR Green RT-PCR Kit, Qiagen) and determination of ΔΔCT-values was done on the CFX Maestro Software (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was reverse transcribed and quantified by real-time PCR using the OneStep RT-PCR kit (Qiagen) as described previously(40 ...