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Citations for Qiagen :
301 - 350 of 2241 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and clarified lysate was incubated with Ni-NTA affinity resin (Qiagen) for 1 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cleared lysates were incubated with 2-4ml nickel NTA beads (Qiagen) for 20-40 minutes before washing beads with 5-10 column volumes of lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The clarified lysate was incubated with Ni-NTA agarose beads (Qiagen) for 1.5 hours ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified from sonicated lysates using Ni-NTA resin (Qiagen) and gravity flow ...
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bioRxiv - Cell Biology 2023Quote: ... and clarified lysate was loaded into Ni-NTA Agarose (Qiagen, USA) gravity column pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was then incubated with NiNTA resin (Qiagen) pre-equilibrated in Buffer A for one hour ...
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bioRxiv - Genetics 2023Quote: ... Cell lysates were homogenized using QIAshredder spin columns (Qiagen, Cat #79656) and genomic DNA was removed by an on-column DNase treatment (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... Lysates were thawed on ice and the remaining steps from Qiagen kit were carried out at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified bacterial lysate was then incubated with Ni-NTA agarose (Qiagen) at 4 °C for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were then homogenized by passing them through QIAshredder cartridges (Qiagen). Proteins were precipitated by adding an equal volume of ice cold 55% Trichloroacetic acid (TCA ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was loaded onto tandem Streptactin XP columns (QIAGEN), equilibrated in Buffer A ...
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bioRxiv - Cancer Biology 2023Quote: ... The lysate was applied to the Ni-NTA agarose resin (Qiagen) and the resin was washed three times using a buffer [20 mM Tris-HCl (pH 8) ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was then purified with Ni-NTA resin (Qiagen) in batch mode ...
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bioRxiv - Microbiology 2024Quote: ... Cleared lysate was bound to Strep-Tactin Superflow Plus resin (Qiagen). The resin was washed first with lysis buffer and then with RNP buffer (20 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Physiology 2023Quote: ... Tissue lysates were further clarified by microcentrifuge spin columns (QIAshredder, Qiagen) to obtain clear protein lysate centrifuged at 10,000g for 2min at 41C ...
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bioRxiv - Molecular Biology 2024Quote: ... The clarified lysate was incubated with 0.5 mL NiNTA resin (QIAGEN) for 1 hr at 4°C on a rotary ...
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bioRxiv - Microbiology 2024Quote: ... cleared lysates were incubated with 2 mL Ni-NTA agarose (Qiagen) on a rotator for 1 hour at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Total nucleic acids were extracted using QIAamp 96 virus Qiacube HT kit on QIAxtractor Automated extraction (Qiagen, US) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: The virus RNA was extracted from clinical samples using the QIAamp viral RNA mini kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: Viral RNA from cell culture supernatants was isolated as the manufacturer’s instructions using QIAamp MinElute Virus kit (Qiagen). Cells were suspended in TRI Reagent (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: Load Viral RNA was isolated from plasma and cerebral spinal fluid (CSF) using the QuantiTech Virus Kit (Qiagen). Viral loads were quantified by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was extracted from 200 µl of the inactivated supernatant using the EZ1 Virus Mini Kit (Qiagen, Germany). As described previously ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from nasopharyngeal swab samples on the QiaSymphony platform using the Virus Pathogen Mini Kit (Qiagen). Libraries were prepared using the Illumina DNA Flex library preparation kit and sequenced on an Illumina MiSeq (V2 ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from 140 µl of virus stock (SARS-CoV-2 VOC Delta, GISAID ID: EPI_ISL_15250227) using a QIAamp Viral RNA Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RNA from virus stocks was extracted with the QIAamp Viral RNA Mini Kit (Cat# 52904, QIAGEN, Hilden, Germany), and one-step cDNA synthesis was then performed with SuperScript IV RT Viral cDNA (Cat# 12594025 ...
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bioRxiv - Immunology 2022Quote: ... 200 μL of nasal swab sample was extracted with the QIAamp 96 Virus QIAcube HT kit (Qiagen, Germany) on the QIAcube HT System (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was extracted from the virus stocks using the QIAamp viral RNA mini kit (Qiagen, Cat#74l36) and viral genomes were sequenced as described before [31].
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bioRxiv - Microbiology 2023Quote: Virus isolates and respiratory specimens were extracted using the QIAgen Viral RNA Mini-Kit (QIAgen, Inc., Valencia, CA). Input sample volume was 140 μl ...
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bioRxiv - Immunology 2024Quote: ... Recombinant DENV EDIII antigens were expressed in mammalian Expi293 cells and purified from the culture supernatant using Ni-NTA agarose (Qiagen). Purified EDIII antigens were biotinylated using HaloTag PEG biotin ligand (Promega) ...
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bioRxiv - Biophysics 2020Quote: ... Cells lysates were treated with 100 μg/mL of RNase A (Qiagen). Lysates were cleared by centrifugation and then incubated with Glutathione-Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... Cleared lysates were then passed through a Ni-NTA column (Qiagen 30250) that was equilibrated with P5 Buffer pH 7 (20 mM NaHPO4 ...
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bioRxiv - Cell Biology 2019Quote: ... the lysates were collected and incubate with Ni-NTA agarose beads (QIAGEN) for 4 hr ...
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bioRxiv - Cell Biology 2019Quote: ... The cleared lysate was batch bound to Ni-NTA agarose beads (Qiagen) for 1 h at 4oC ...
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bioRxiv - Developmental Biology 2021Quote: ... before the lysate was recovered and passed through a QIA shredder (Qiagen). An equal volume of 100 % ethanol was added to the homogenised lysate before being transferred to a RNeasy extraction column ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were loaded onto a 5 mL Ni-NTA Superflow Cartridges (Qiagen) equilibrated with Buffer B (25 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was incubated with Ni-nitrilotriacetic acid (NTA) resin from Qiagen for 1hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was then subjected to Ni-NTA affinity chromatography (Qiagen) and washed with wash buffer (Na2HPO4 pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was then loaded onto Ni-NTA spin columns (QIAgen) and proteins purified according to manufacturer protocol washing the column with Washing Buffer (30 mM Hepes pH 7.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... QIAshredder homogenizer columns were used to disrupt the cell lysates (Qiagen, 79654). mRNA was purified using the NEBNext poly(A ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA was extracted from cell lysates using RNAeasy purification kit (Qiagen). The quality of RNA was assessed on the Agilent 2100 Bioanalyzer and the RNA was quantified by QuBit fluorometry ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was loaded on a Ni-NTA affinity column (Qiagen). The bound proteins were first washed with lysis buffer and then with the lysis buffer supplemented with 20 mM imidazole to remove non- specific proteins ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was loaded on a Ni-NTA affinity column (Qiagen) and the column was then washed with the lysis buffer and 50 mM imidazole buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and the cleared lysate was incubated with Ni-NTA agarose beads (Qiagen). The protein complex was eluted with buffer containing 30 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... and the cleared lysate was incubated with Ni-NTA agarose beads (Qiagen). After several washing steps in wash buffer (50 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... Lysates were processed using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen). To generate sample libraries ...
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bioRxiv - Microbiology 2022Quote: ... RNA extraction on the lysate was performed with RNeasy Mini Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed with lysis buffer supplemented with 25 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed in two steps with lysis buffer supplemented with 25 mM and 100 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was incubated with 800 μL of Ni-NTA agarose (Qiagen) for 1 hour at 4°C ...