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Citations for Qiagen :
3301 - 3350 of 4034 citations for 7 trifluoromethyl 1 2 3 4 tetrahydro 5 nitroisoquinoline hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cancer causes dysfunctional insulin signaling and glucose transport in a muscle-type specific mannerbioRxiv - Physiology 2021Quote: ... Muscles were homogenized 1 min at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in 300 μl ice-cold homogenization buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA synthesis was performed from 1 μg of total RNA using the Omniscript RT Kit (Qiagen). All the samples were analyzed in triplicate using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Microbiology 2019Quote: ... and cell-associated HSV-1 DNA was isolated with the QIAamp DNA Blood Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... ulcerans CU001 strain calibrated at 1 McFarland = 106 CFU was extracted with EZ (Qiagen, Hilden, Germany). Then ...
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bioRxiv - Microbiology 2019Quote: cDNA from 1 µg of RNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: 1 µg of the total RNA was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized from 1 μg of total RNA using QuantiTect® reverse transcription kit (Qiagen) in 20 μl reaction volume ...
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bioRxiv - Biophysics 2022Quote: ... were filled with ( 1 mL | 0.5 mL ) of bed Ni-NTA resin (Qiagen or Thermo Fisher), and equilibrated with three rinses of Wash buffer (at least 30 resin bed volumes ...
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bioRxiv - Cell Biology 2022Quote: ... run on a 1 % agarose gel and gel extracted using QIAquick Gel Extraction Kit (#28704, Qiagen). tdTomato and neomycin were cloned out from H3.1-miCOUNT (Denoth-Lippuner et al. ...
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bioRxiv - Cell Biology 2022Quote: ... run on a 1 % agarose gel and gel extracted using QIAquick Gel Extraction Kit (#28704, Qiagen). The plasmid backbone ...
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bioRxiv - Microbiology 2021Quote: ... and 1 μg of linearized DNA was transfected into A549 cells using Effectene Transfection Reagent (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... pyrosequencing was conducted on a PyroMark Q96 MD instrument using CpG LINE-1 assay (973043, Qiagen). To monitor bisulfite conversion efficiency ...
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bioRxiv - Immunology 2022Quote: ... freshly sorted G1-ILC populations (1 × 103 cells) were lysed with Buffer RLT (Qiagen, Hilden, Germany) and purified with RNAClean XP (Beckman Coulter ...
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bioRxiv - Physiology 2022Quote: ... was extracted from adipose tissue (experiment 1) using the Dneasy Blood and Tissue Kit (Qiagen, # 69504). We amplified both the mouse genomic gene Glyceraldehyde 3-phosphate dehydrogenase (Gapdh ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was collected and tumbled with 1 mL of preequilibrated Ni(II)-NTA Superflow (Qiagen) resin for 1-1.5 h at 4 °C.
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were purified on a 1% gel and extracted using QIAquick Gel Extraction Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNAs were prepared in RLT RNA lysis buffer supplemented with 1:100 β-mercaptoethanol (Qiagen) at 5,000 cells/µl ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1 mg of RNA was reverse transcribed to cDNA using the Omniscript RT Kit (Qiagen). qPCR was performed using the TaqMan assay system ...
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bioRxiv - Immunology 2020Quote: ... Disruption of microbial cells was enhanced using TissueLyser II (QIAGEN, 30 shakes/sec., 1 min., 2x). To lyse Gram-negative bacteria ...
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bioRxiv - Microbiology 2020Quote: ... 1 ug of RNA was reverse transcribed to cDNA with the QuantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 mL of each culture was mixed with 1 mL of RNA Bacteria Protect Reagent (Qiagen) immediately after 2 mM ZnCl2 induction (t0 ...
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bioRxiv - Genomics 2021Quote: ... size-selected on a 1% agarose gel and purified using the MinElute PCR Purification Kit (Qiagen). The DNA library was then cloned into the vector using four In-Fusion cloning reactions according to the manufacturer’s instructions (Clontech In-Fusion HD) ...
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bioRxiv - Genomics 2021Quote: ... size-selected on a 1% agarose gel and extracted with the QIAquick Gel Extraction Kit (Qiagen). The eluates were pooled and purified using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2019Quote: Genomic DNA from 1 g leaf material was isolated using the DNeasy Plant Maxi kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... RNA was extracted from THP-1 and cDNA was synthesized using RT2 First strand kit (Qiagen). Pre-designed RT2 qPCR primer assays (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 ml of PE buffer and 100 μl of EB buffer (MinElute PCR Purification Kit, QIAGEN).
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bioRxiv - Genetics 2021Quote: ... Replicates were lysed in 1 mL of RLT buffer and homogenized using QIAshredder columns (Qiagen, 79654). Total RNA was extracted and eluted in 100 uL RNase-free water using an RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL for OD600 ~0.4 for 10% CO2) and stabilized by RNA Protect Bacteria Kit (Qiagen). Next ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA from A549 cells (1 × 106 cells) was extracted using miRNeasy Mini kit (Qiagen, Denmark) according to the manufacturer’s instructions and eluted in 60 µl of nuclease free water ...
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bioRxiv - Immunology 2022Quote: ... at 50 °C for 1 hour and were purified with a MinElute PCR Purification Kit (QIAGEN).
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bioRxiv - Microbiology 2022Quote: ... HSV-1 DNA within cells was isolated from frozen pellets using QIAamp DNAMini Kit (Qiagen: 51304).
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of RNA was used for cDNA synthesis with the QuantiTect Reverse Transcription kit (Qiagen). Samples were diluted 2.5x after cDNA synthesis and SYBR™ Select Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from approximately 1 million cells per condition using DNA extraction kit (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... and 200 µM VLCFA mix for 1 d using the RNeasy Plant kit (QIAGEN, Hilden, Germany). For isolation of RNA from the bending site of roots ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: Bulk or flow-sorted THP-1 macrophages were lysed with β-ME-supplemented Buffer RLT (Qiagen). RNA was isolated from the lysate using an RNEasy plus kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells were transfected with 1 μg of pUbg-R252 plasmid using Effectene transfection reagent (QIAGEN) in a 60 mm dish following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 1 mL of PE buffer and 100 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). The silica columns were placed in a collection tube to dry and later in a 1.5 mL DNA lo-bind tube (Eppendorf ...
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bioRxiv - Immunology 2024Quote: ... 1 mL of PBS-AB was added to each tissue with Tungsten carbide 3mm beads (Qiagen). Samples were homogenized for 15 min and then centrifuged at 15,000 g for 10 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from frozen cell pellets of 0.3-1×106 PBMCs (Qiagen, Germantown, MD), and concentrated using an ethanol precipitation protocol with GlycoBlue following manufacturer’s instructions (Thermofisher ...
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bioRxiv - Systems Biology 2023Quote: Small cfRNAs were extracted from ~1 mL of plasma using the miRNeasy Serum/Plasma Kit (Qiagen). 1ul ExiSEQ NGS Spike-in (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... RNAs were converted to cDNA using 1 μg RNA and a QuantiTect Reverse Transcription kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Reverse Transcription was performed on 1 µg of RNA using a QuantiTect Reverse Transcription Kit (Qiagen), and qPCR was performed using a QuantiNova SYBR Green PCR kit (Qiagen) ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 1 mL buffer RLT from the RNeasy mini kit (Qiagen, Germany) with a 1:200 dilution of β-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was clarified by centrifugation and incubated with 1 mL Ni-NTA resin (Qiagen). The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Genomics 2023Quote: ... with each 20 μL reaction consisting of 1× QuantiFAST reaction mix containing ROX reference dye (Qiagen), 0.66 µM forward and reverse primers ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour at room temperature followed by isolation with a minElute RNA Cleanup Kit (Qiagen). RNA concentrations were measured by Nanodrop and total RNA integrity assayed using an Agilent Bioanalyzer ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total mRNA from each sample was retrotranscribed using QuantiTech reverse Transcription Kit (Qiagen). Real-Time PCR was performed using SYBR Green Master Mix (Bio-Rad ...