Labshake search
Citations for Qiagen :
3151 - 3200 of 3900 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... and 1 mL of QIAzol lysis reagent (Qiagen, item #79306). Tissue homogenization was done by agitating the tubes for 20□s at a 7□m□s−□1 speed (Beadbug 6 homogenizer ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 30 μl of 20 mg/ml Proteinase K (Qiagen) to 9 million cells (>500 coverage ...
-
bioRxiv - Genomics 2019Quote: ... and incubated in PBS + 100ug/mL RNase A (Qiagen 19101) for 1 hour at 37°C ...
-
bioRxiv - Genomics 2021Quote: ... then washed twice with 750 mL Buffer PE (Qiagen #19065). The sample was then eluted in 40 uL of Buffer EB (10 mM Tris pH 8 ...
-
bioRxiv - Bioengineering 2022Quote: ... 15 μl of Proteinase K (20 mg/ml; Qiagen #19157) were added to the suspension and incubated at 65°C for approximately one hour ...
-
bioRxiv - Biochemistry 2022Quote: ... was passed through a 1 mL NiNTA-agarose column (Qiagen) preequilibrated with the TRIS/urea buffer ...
-
bioRxiv - Cancer Biology 2022Quote: ... supplemented with 30 µl 20mg/ml Proteinase K (Qiagen 19131), and incubated at 55°C overnight ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1 ul of 100 mg/ml RNase A (Qiagen, 19101) was added after Proteinase K digestion at 55°C for a further one-hour incubation at 37°C to degrade all RNA ...
-
bioRxiv - Immunology 2023Quote: ... and 30 μL of 20 mg/mL Proteinase K (Qiagen) were added to the tissue ...
-
bioRxiv - Microbiology 2022Quote: ... and 1 μL of 10 mg/mL RNase A (Qiagen), then incubating at 37°C for 1.5 hr without shaking ...
-
bioRxiv - Cell Biology 2023Quote: ... Volumes were adjusted to 20 ml using buffer EB (Qiagen). Samples were then reverse-crosslinked by incubating them at 50 °C for 30 min ...
-
bioRxiv - Immunology 2023Quote: ... and 30 μl of 20 mg/ml Proteinase K (Qiagen) were added to the tissue/cell sample and incubated at 55°C overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... and a 1 mL slurry of Ni-NTA beads (Qiagen) pre-equilibrated with lysis buffer was added ...
-
bioRxiv - Microbiology 2023Quote: ... Each sample was immediately treated with 10 mL RNAprotect (Qiagen) and incubated at room temperature for 5 min ...
-
bioRxiv - Genetics 2023Quote: ... Lysate was passed over 8 ml Ni-NTA resin (Qiagen), washed with 70 ml wash buffer (20 mM HEPES-KOH pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... 6 mL of Ni2+-NTA slurry (Qiagen, Venlo, LI, Netherlands) were equilibrated in a gravity flow column with Wash Buffer (50 mM Tris-HCl ...
-
bioRxiv - Genomics 2023Quote: ... After adding 10 µL RNase A (100 mg/mL, Qiagen) and 250 µL Proteinase K (20 mg/mL ...
-
bioRxiv - Microbiology 2022Quote: ... Thawed cells were suspended in 1 ml RLT buffer (Qiagen) containing 2-mercaptoethanol and lysed by bead beating ...
-
bioRxiv - Microbiology 2023Quote: ... supernatant was poured over 8 mL Ni-NTA resin (Qiagen), resin was washed with 35 mL lysis buffer supplemented with 1 M NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... Cleared extract was loaded onto 1 ml NiNTA resin (Qiagen) preequilibrated with Nickel buffer (25 mM HEPES-NaOH pH 7.4 ...
-
bioRxiv - Cell Biology 2023Quote: ... Specimens were then treated with 1mg/ml RNAse A (Qiagen) in PBS for 30 minutes at 37°C and stored in 25% glycerol/PBS at 4°C ...
-
bioRxiv - Systems Biology 2023Quote: ... 7.5 mL of pre-cooled buffer P3 (QIAGEN, Düren, Germany) were added and the samples were again homogenized by inverting the tubes several times ...
-
bioRxiv - Immunology 2023Quote: ... immersed in 0.5 mL of RNA-later (Qiagen, Hilden, Germany) and stored at -80°C ...
-
bioRxiv - Biochemistry 2024Quote: ... pellets were resuspended in 1 ml QIAzol Lysis Reagent (Qiagen) and mixed with 250 µl of zirconia beads (Roth) ...
-
bioRxiv - Bioengineering 2024Quote: ... Gravity columns containing 2.5 mL Ni-NTA (Qiagen, Catalog # 30210) were pre-equilibrated with >10 column volumes (CV ...
-
bioRxiv - Genomics 2024Quote: ... DNase-free RNAse A (100 mg/ml) was from Qiagen, Proteinase K ...
-
bioRxiv - Biochemistry 2024Quote: ... and 1 μL of 10 mg/mL RNase A (Qiagen) was added ...
-
bioRxiv - Immunology 2021Quote: ARNO siRNA (Mm_Pscd2_3) or a negative non-specific siRNA control (Allstar siRNA) were transfected into SFs using HiPerFect transfection reagent (all Qiagen,UK) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2020Quote: ... 0.5 µl of diluted template cDNA (1:3, nuclease-free water) per real-time PCR reaction (10 µl – SYBR Green PCR kit, Qiagen, 204143) was used to assay specific transcript abundance (CFX96 Real-Time System ...
-
bioRxiv - Genomics 2022Quote: ... genomic DNA was extracted from 3-week-old leaves of ALO seedling using the QIAGEN® Genomic DNA Extraction Kit (Cat. 13323, Qiagen) according to the manufacturer protocol ...
-
bioRxiv - Neuroscience 2019Quote: ... Synthesis of cDNA was performed on 0.3-1.0 μg of normalized total RNA from each sample using QuantiTect Reverse Transcription kit (Cat No. 205313, Qiagen, Valencia, CA) which included a DNase treatment step to remove any residual genomic DNA contamination ...
-
bioRxiv - Genomics 2021Quote: ... DNA corresponding to the size of mononucleosomes (100-200 bp) was re-isolated from a 3% agarose gel using MinElute Gel Extraction Kit (Qiagen, # 28604). As input ...
-
bioRxiv - Immunology 2021Quote: ... RNA was extracted from homogenized lysate containing a range of 3×103 to 1×105 cells per sample using a ll Prep DNA/RNA Mini kit (Qiagen, #80204). RNA was purified and concentrated using RNAClean XP beads (Beckman Coulter ...
-
bioRxiv - Neuroscience 2020Quote: ... The following primers were used to assess the relative level of GAT-3 mRNA: Slc6a11 Rn.10545 in comparison to that of cyclophilin A (Peptidylprolyl isomerase A) used as housekeeping gene (Qiagen, UK). Real-time-PCR was performed on the CFX96 Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Cancer Biology 2021Quote: ... 200 μL of plasma/EV suspension were mixed with 1000 μL Qiazol and 1 μL of a mix of 3 synthetic spike-in controls (Qiagen, Germany). After a 10-minute incubation at room temperature ...
-
bioRxiv - Systems Biology 2020Quote: RNA was isolated from 3 biological replicates of each U2OS time point according to manufacture instruction using the RNeasy Plus Mini Kit (QIAGEN, #74134). Isolated RNA was reversely transcribed by using first-strand cDNA synthesis kit (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was performed in an Applied Biosystems QuantStudio 3 thermocycler using the QuantiNova Probe SYBR Green PCR Kit (Qiagen, Germany); mix proportions and cycling parameters were used as described in manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: 1-3 million of feeder-independent mESCs were harvested for genomic DNA extraction by using a QIAamp DNA Mini Kit (QIAGEN, 51306). Genomic DNA concentration was determined with a Nanodrop spectrophotometer ...
-
bioRxiv - Cell Biology 2022Quote: ... and organoids at the end of passage one subjected to two weeks of differentiation (n=3) using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... 40 μl of media from each of 6 plant wells or media from 3 minimal media wells were pooled and stabilized in RNAprotect® Bacteria Reagent (QIAGEN) before performing RNA extraction using RNeasy Mini Kit (QIAGEN) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and RNA was purified after 3 days from the second transfection using the RNeasy Plus Mini Kit (Qiagen, Catalog number 74134) following the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2022Quote: ... Cells from 3 independent biological replicates of MG1655 in each modified medium were treated with RNA protect bacteria reagent (Qiagen, Germany), to prevent degradation of RNA ...
-
bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from the stool of enrolled mice (3 mice per group) using the QIAamp DNA Stool Mini kit (Qiagen, Germany). The extracted DNA was amplified using primers targeting the V1 to V3 hypervariable regions of bacterial 16S rRNA gene (27F ...
-
bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from aerial parts of 3-week-old seedlings grown on soil using either RNeasy Plant Mini Kit (Qiagen, 74904) or Monarch Total RNA Miniprep Kit (NEB ...
-
bioRxiv - Biochemistry 2021Quote: ... RT-PCR products were gel purified in 3% agarose gel and cleaned up using QIAGen gel extraction kit (QiaGen, Hilden, Germany).
-
bioRxiv - Cell Biology 2021Quote: ... The plasmid DNA was extracted from 3-9 positive colonies where possible after blue-white screening using a QIAprep® Spin Miniprep Kit (Qiagen) and sequenced using the standard T7 primer at Eurofins Genomics ...
-
bioRxiv - Cancer Biology 2022Quote: ... and GSC6-27 HEPACAM shRNA (n=3) cultures were washed in cold 1X PBS and total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, 74134) following manufacturer’s guidelines ...
-
bioRxiv - Biochemistry 2022Quote: ... Trimming of the obtained sequencing reads and mapping to the pea aphid genome (version 3) were performed with the program CLC Genomics Workbench (Qiagen Bioinformatics) (mapping parameter ...
-
bioRxiv - Bioengineering 2022Quote: ... A cell mass of an equivalent of a OD600 3 was used for a DNA mini preparation using a QIAprep® Spin Miniprep Kit (Qiagen). DNA was eluted in 50 μL elution buffer and measured using a NanoDrop one (Thermo Fisher Scientific).
-
bioRxiv - Genetics 2022Quote: ... and non-inoculated G305-3M leaf segments collected along 10 different time points (0, 3, 6, 9, 12, 16, 24, 36, 48, 72 hpi) using the RNeasy Plant Mini Kit (Qiagen, Germany). The cDNA was synthesized from total RNA using a qScript™ cDNA Synthesis Kit (Quantabio ...