Labshake search
Citations for Qiagen :
3051 - 3100 of 10000+ citations for C Reactive Protein ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: RNA was isolated from CD3+ T-cells using the Qiagen RNeasy Plus Mini Kit or EN AllPrep Mini Kit with on column DNaseI (Qiagen) digestion ...
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bioRxiv - Immunology 2020Quote: RNA was isolated from T cells as indicated using the RNeasy Micro Kit and then reverse-transcribed to produce cDNA using oligonucleotide priming with the QuantiTect Reverse Transcription Kit (both Qiagen). Quantitative PCR was performed using the QuantiTect SYBR green PCR Master mix (Roche ...
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bioRxiv - Genetics 2019Quote: Total RNA was isolated from germinating yeast spore cultures by the “mechanical disruption protocol” using RNeasy MIDI kit (for RNA-Seq) or RNeasy mini kit (for Real-Time qRT-PCR) (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... RNA was isolated from chondrocytes using the RNeasy Mini Kit and from connective tissue using the RNeasy Fibrous Tissue Mini Kit (Qiagen). All other tissues were first homogenized using Lysing Matrix S ...
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bioRxiv - Immunology 2022Quote: ... genomic DNA was isolated 3 or 8 days after editing by a QIAamp DNA Mini Kit or Micro Kit (QIAGEN). The genomic region flanking the CRISPR/Cas9 cleavage site was PCR-amplified ...
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bioRxiv - Microbiology 2022Quote: Total DNA was extracted from bulk (n = 23) and rhizosphere (n = 23) soils using the PowerSoil DNA Kit or the PowerSoil Pro Kit (QIAGEN) following the manufacturer’s protocols with minor modifications ...
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bioRxiv - Microbiology 2022Quote: ... Total DNA and RNA were extracted from ~1.5 g of rhizosphere soil using the RNA PowerSoil Kit with the DNA elution kit (Qiagen, Germany). DNA and RNA were extracted using ~0.03 g of roots using the DNeasy Plant DNA Extraction Kit ...
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bioRxiv - Pathology 2024Quote: RNA from cells was isolated using the RNeasy Mini Kit or RNeasy Micro Kit (74104 and 74004, respectively, QIAgen, Germany), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Verified plasmids were extracted from Escherichia coli (E. coli) using the QIAprep Spin Miniprep Kit or HiSpeed Plasmid Midi Kit (Qiagen) and dialyzed (0.025µM MCE Membrane ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from the purified oocysts using the Qiagen DNA Mini Kit and amplified using the REPLI-g Midi Kit (QIAGEN). WGA products were purified using the EasyPure PCR Purification Kit (TransGen Biotech ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from RPE/choroid complex and primary RPE cells using a RNA extraction kit (RNeasy plus mini kit QIAGEN). The RNA concentration and integrity were determined with NanoDrop 1000 spectrophotometer ...
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bioRxiv - Molecular Biology 2024Quote: ... About 20 μg of grinded tissue were used to extract DNA using the blood and tissue kit or the Gentra Puregene tissue kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA and total RNA were isolated from these tissues using QIAamp DNA mini kit and RNeasy mini kit (Qiagen) respectively ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from bacterial cells using the RNeasy mini kit (5 mL culture) and RNeasy maxi kit (50 mL culture) (Qiagen). RNA concentrations were measured using a Nanodrop spectrometer (ThermoScientific) ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from cDCs using the miRNeasy mini kit with the inclusion of the genomic DNA digestion step with the RNase-free DNase Kit (Qiagen). RNA quality was assessed by the Bioanalyzer RNA Nano Chip and depletion of rRNA prior to library generation was performed using RiboErase selection kit (Cat.# KK8561 ...
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bioRxiv - Pathology 2023Quote: ... DNA was extracted (DNeasy Blood and Tissue Kit/ QIA amp DNA FFPE tissue kit, QIAGEN; BCA Dept. University of Padua) from both GAF and NBF-fixed lung ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Real-Time PCR was performed with QuantiNovaTM SYBR® Green PCR Kit following the instruction manual of the kit (QIAGEN) on a LightCycler 480 real-time PCR system (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... The total RNA was isolated from each slice separately using an adapted protocol from RNeasy Mini Kit (RNeasy Mini Kit #74104, Qiagen). Each slice was placed into a 2ml low binding tube (DNA LoBind® #0030108078 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Genomic DNA and plasmids were extracted using the Fast Pure Bacteria DNA isolation mini kit (Vazyme) and the QIAprep Spin Miniprep Kit (Qiagen), respectively ...
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bioRxiv - Immunology 2023Quote: ... amplification of TCRβ-chain transcripts and library preparation was performed with the arm-PCR (ampliconrescued multiplex PCR) technology (iRepertoire Inc. Huntsville, USA) using the Qiagen OneStep RT-PCR Kit and Qiagen Multiplex PCR Kit (both Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... MDA controls were performed employing a random-primers-based MDA (RP-MDA) kit as random-primer-based method: Repli-g Mini Kit from QIAGEN; and a TruePrime-based-MDA (PrimPol-MDA ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated using the Qiagen RNeasy kit and cDNA was generated by using the QuantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... was reverse transcribed into cDNA by miScript RT II Kit using miScript HiSpec Buffer or miRCURY LNA RT Kit (Qiagen). Expression levels of miR-142-5p were analyzed using miScript PCR System or miRCURY LNA SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by ribosomal RNA depletion using the NEBNext rRNA Depletion Kit (E6310) or the QIAseq FastSelect -rRNA HMR Kit (Qiagen). RNA sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (E7760 ...
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bioRxiv - Microbiology 2023Quote: ... A 25 mg lyophilised sample of feces was used for extraction of total bacterial DNA using a commercial kit (QIAamp DNA stool kit; Qiagen). 16S amplicon sequencing of the V3-V4 region was performed on Illumina MiSeq (2 × 300 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... Total mRNAs were extracted using the Macherey-Nagel RNA easy extraction kit and quantified by qRT-PCR using the QuantiTect SYBR Green qRT-PCR kit (Qiagen) and appropriate primers ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from frozen pellets and caecum tissue using the NucleoSpin RNA extraction kit (Machery-Nagel, #740955.5) or the RNeasy Plus Mini Kit (Qiagen, #74134). Equal concentrations of RNA (measured by NanoDrop and 1 µg for the caecum tissue ...
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bioRxiv - Microbiology 2023Quote: ... DNA and RNA were extracted from 1.4 – 2.7g of each sample using the RNeasy PowerSoil Total RNA kit and DNA elution accessory kit (Qiagen, Hilden, Germany) according to an optimised version of the manufacturer’s instructions with an added heat block step (45°C for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... RNA and DNA was co-extracted using a combination of the RNeasy PowerSoil Total RNA Kit and the RNeasy PowerSoil DNA Elution Kit (Qiagen), and then further processed for long read nanopore sequencing.
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bioRxiv - Biochemistry 2023Quote: Total RNA was isolated and purified from cultured cells using a standard commercial RNA prep kit (#48500, Norgen) and 1 μg of RNA was reverse transcribed to cDNA using a cDNA synthesis kit (#205314, Qiagen). 10 ng of cDNA was used for quantitative PCR by SYBR Green ...
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bioRxiv - Immunology 2023Quote: ... Samples were enriched for CD45+ cells using an EasySep Mouse TIL (CD45) Positive Selection kit (STEMCELL) and RNA was extracted with an RNeasy Plus Mini Kit (Qiagen). TdLN samples were processed as previously described ...
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bioRxiv - Immunology 2023Quote: ... Samples were again enriched for CD45+ cells using an EasySep Mouse CD45 Positive Selection kit (STEMCELL) and RNA was extracted with an RNeasy Plus Mini Kit (Qiagen). RNA was stored at −80°C until further processing.
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bioRxiv - Developmental Biology 2023Quote: ... Snap frozen PGC pellet was directly lysed using the LyseAll Lysis Kit (part of the EpiTect FAST Bisulfite Conversion Kit, Qiagen) and was bisulfite treated using the standard Epitect Bisulfite reagent mix following the low-input protocolPGC bisulfite-treated DNA was resuspended in 20 μL of 1:10 of the supplied EB buffer.
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bioRxiv - Evolutionary Biology 2023Quote: ... We isolated DNA from individual nematodes using the Nucleospin Tissue kit (MN) or DNeasy Blood and Tissue DNA extraction kit (Qiagen). Edinburgh Genomics prepared Illumina Nextera libraries for two individuals (Hm2 and Hm16) ...
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bioRxiv - Genetics 2023Quote: ... DNA from formalin-fixed paraffin-embedded (FFPE) tumor samples was isolated with the kit QIAamp DNA FFPE tissue Kit (Qiagen). All extractions were carried out following the manufacturers’ instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... we extracted genomic DNA from ethanol-preserved liver tissue using the DNeasy Blood and Tissue kit or the DNeasy Blood and Tissue QIAcube kit (Qiagen). For the high elevation mummy samples ...
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bioRxiv - Cancer Biology 2023Quote: ... Correctly assembled UniSAM and pLV hU6-sgRNA hUbC-dCas9-KRAB- T2a-GFP vectors were confirmed by complete Sanger sequencing using BigDye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystem, #4337455) and DyeEx 2.0 Spin kit (Qiagen, #63204) according to manufacturer recommendation and analyzed on ABI3500xL (Applied biosystem ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from the FFPE tissue samples using a commercially available extraction kit (RNeasy FFPE kit, Qiagen, Germany), following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was extracted using the NucleoSpin RNA II kit (Machery & Nagel) and the cDNA were synthesized using the QuantiTect Reverse Transcription Kit (Qiagen). cDNA samples were run in triplicates and experiments were repeated with independently isolated RNA samples ...
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bioRxiv - Neuroscience 2023Quote: ... and total RNA was extracted and purified from an aliquot of the tissue using the miRNeasy Mini Kit protocol and DNase Kit (Qiagen). A mixture of all subregions were included in the tissue aliquot used for RNA isolation ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was extracted from all the cell lines using RNA extraction kit (AllPrep DNA/RNA/miRNA Universal Kit, QIAGEN). One microgram of total RNA was reverse-transcribed using each miRNA-specific stem-loop primer (Supplementary table 1) ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA extraction included on-column treatment with DNase I following the optional portion of the RNeasy kit using the DNase I kit (Qiagen).
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bioRxiv - Pathology 2024Quote: Total RNA was extracted from cell lysates using EZNA RNA extraction kit I or from blood samples using QIAamp Viral RNA Mini Kit (Qiagen). Absolute quantification of positive strand gRNA was performed through one-step RT-qPCR ...
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bioRxiv - Immunology 2024Quote: ... The pellet was resuspended in the lysis buffer provided in the kit and cells were fractionated using Qproteome-mitochondria isolation kit according to manufacturer’s instructions (Qiagen 37612).
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bioRxiv - Plant Biology 2020Quote: ... using the RNeasy Mini Kit (Qiagen, Hilden, Germany). The extractions were performed from tissues of plants treated with the foliar Zn doses that produced a significant increase in Zn concentration in shoots (0.1 ...
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bioRxiv - Microbiology 2020Quote: ... and purified using a Qiagen RNeasy kit (QIAGEN). The RNA was reverse transcribed into cDNA using an iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Rywal plants using RNeasy Plant Mini kit (Qiagen). Residual genomic DNA was digested with DNase I (Qiagen) ...
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Vegetative nuclear positioning is required for calcium and ROS signaling in Arabidopsis pollen tubesbioRxiv - Plant Biology 2020Quote: ... purified with the QIAquik PCR Purification kit (Qiagen) and subsequently ligated into a pH2GW7 vector (Takagi et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... and PCR purification kits were obtained from QIAGEN. All primers and oligos were synthesized from Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... the plasmid was extracted using Maxiprep kit (Qiagen).