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Citations for Qiagen :
2951 - 3000 of 4234 citations for 6 chloro 2 N 2 N diethyl 4 N propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Colony PCR was performed with KOD One™ PCR Master Mix (Toyobo) to screen colonies before recovering plasmids with a 5 mL overnight culture and miniprep (QIAgen).
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA was extracted from 5- or 7-day post-fertilization (dpf) stage animals using RNeasy Mini Kit (Qiagen, cat. 74104) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Transfection was conducted with 5 μg plasmids (act-GAL4, UAS-lbm-GFP, UAS-upd2-mCherry) using Effectene Transfection Reagent (QIAGEN, #301425).
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were plated in 10 cm plates at 5 million cells/plate and the next day transiently transfected with the indicated plasmids using PolyFect (Qiagen 301105) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and 30 uL/well 1 X lysis buffer (1x Qiagen TCL, 1% beta-mercaptoethanol) was added ...
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bioRxiv - Biophysics 2022Quote: ... The YB-1 (1-180) protein fragment was purified following the manufacturer’s recommendations (Qiagen). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... supernatant was combined with Ni-NTA resin (1 mL/1 L of biomass, Qiagen) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Genomics 2023Quote: ... slides were treated with 1 mg/ml RNase A (1 mg/ml, Qiagen, 19101) in 2x SSC for at least 45 min at 37°C and then dehydrated in a 70% ...
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bioRxiv - Molecular Biology 2021Quote: ... 1× QuantiNova RT Mix (Qiagen), 1× QuantiNova SYBR Green RT-PCR Master mix ...
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bioRxiv - Microbiology 2021Quote: ... Strep 1:1000 (Qiagen, 34850).
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bioRxiv - Evolutionary Biology 2022Quote: ... 1 μM oligo-dT18 (Qiagen) and 10 μM random hexamers (Promega).
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bioRxiv - Epidemiology 2019Quote: ... version 1 (Qiagen, Hilden, Germany) with the automated instrument QIAsimphony (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... 1% SDS (Qiagen P2 buffer). Neutralize lysis buffer by adding 300 μL of 4.2 M Guanidine HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μl of DNase (Qiagen) was added ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of QIAzol (Qiagen) was added to each pellet suspension before being transferred to Lysing Matrix B tubes (MP Biomedicals) ...
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bioRxiv - Microbiology 2023Quote: ... and 1 μM TSO (Qiagen). The master mix was dispensed using the MANTIS liquid dispenser followed by mixing for 1 min at 1800 rpm on a plate shaker (Biosan) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 U Hotstar Taq (Qiagen), 1X Hotstar Taq buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 1% SDS (Qiagen P2 buffer). Neutralize lysis buffer by adding 300 μL of 4.2 M Guanidine HCl ...
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bioRxiv - Plant Biology 2022Quote: ... 5’ and 3’ untranslated regions and open reading frames) for the paralogs were made using the CLC Main workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Sequence alignments were generated for the genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... 27620, 1:2,000 dilution) (rabbit anti-Spike S1, Cell Signaling, Cat #: 99423, 1:2,000 dilution) (mouse anti-Strep, Qiagen, Cat #: 34850, 1:2,000 dilution) (rabbit anti-ORF8 ...
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bioRxiv - Genetics 2020Quote: ... placed in 2ml Eppendorf (Hamburg, Germany) tubes and disrupted with a pre-cooled Stainless-Steel Beads (5 mm) in the TissueLyzer (QIAGEN, Venlo, Netherlands). The genomic DNA (gDNA ...
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bioRxiv - Genetics 2020Quote: ... were washed with 5 ml PBS twice and the total RNA was isolated using a RNeasy Mini Kit (74104; Qiagen, Hilden, Germany). The solution was subsequently treated with DNase I (79254 ...
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bioRxiv - Microbiology 2019Quote: ... A total of 25 cycles of PCRs were performed and the PCR products (∼600 bp for 5’-RACR PCR or ∼500 bp for gene-specific PCR) were gel purified (Qiagen, Valencia, CA). A degenerate primer (SAGGTGCAGCTGGTGCAGTCTGG ...
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bioRxiv - Microbiology 2019Quote: ... Amplicons from at least 5 independent PCR products for each sample were pooled together and then purified using the QIAquick PCR purification kit (Qiagen, Hilden, Germany). Whenever semi-nested PCR reactions yielded amplicons ...
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bioRxiv - Biochemistry 2020Quote: Cells seeded in 6 well plates were grown in 5% FBS to a confluency of 70% before RNA extraction using the RNEasy mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... and extracted and amplified from vitrified blastocysts by thawing in phosphate-buffered saline supplemented with 5% bovine serum albumin and processing with REPLI-g whole genome amplification methods (Qiagen, Cat. #150343). Informed consent was obtained from all participants (custodians of the blastocysts ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis of 100 ng total RNA was performed with miRCURY LNA RT Kit (10 µl volume reaction) which adds a 5’ universal tag of a poly(A) tail to mature miRNA templates (QIAGEN, Germantown, MD). cDNA template was diluted 1:10 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were pelleted at 500 g for 5 min and total RNA was extracted using RNeasy Mini Kit (QIAgen, Cat No. 74104), including DNAse digestion step (QIAgen ...
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bioRxiv - Neuroscience 2021Quote: ... tMCAO mice at 24 h and 48 h post reperfusion were used for total mRNA isolation according to the protocols of the Isol-RNA Lysis Reagent (5 PRIME, Gaithersburg, USA) and the bead-milling TissueLyser system (Qiagen, Hilden, Germany). QuantiTect® Reverse Transcription Kit (Qiagen ...
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bioRxiv - Cancer Biology 2019Quote: ... The long genomic DNA was isolated from 5-10mg tumor core using Gentra Puregene Tissue Kit following the manufacturer’s instructions (Qiagen, Cat. No 158667). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR strips were then incubated for 15 min at 37 °C in a thermocycler and the reaction was stopped by adding 5 μl RLT plus buffer (Qiagen, Cat# 1053393) to each well ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining lysate was then transferred to 15 ml tubes containing 5 ml lysis buffer and 150 μl Ni-NTA agarose beads (Qiagen, Venlo, Netherlands) and incubated for 4 h at room temperature under constant mild agitation ...
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bioRxiv - Genomics 2022Quote: ... The mixture was centrifuged at 13,000 rpm for 5 mins and 140 µl of supernatant used for RNA extraction (Qiagen viral RNA kit). The final TNA was eluted in 60 µl of molecular grade water and stored at −80°C.
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bioRxiv - Biochemistry 2023Quote: ... gDNA was isolated from 5 x 106 Colo205 cells using the Blood and Cell Culture DNA Maxi Kit (Qiagen, catalog no. 13362) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... unexpanded leaves was extracted from 14 individuals (7 early-blooming, 5 late-blooming, and the two parents) with a DNeasy Mini Plant Kit (Qiagen, Hilden, Germany) and quantified with a Quant-iTTM PicoGreenTM dsDNA assay kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... a 1 mL aliquot was sterile filtered and kept to test conditioned media for IL-10 induction (as above)) and replaced with 5 mL of RNA-protect (Qiagen, Cat. # 1018390), mixed by pipetting ...
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bioRxiv - Plant Biology 2023Quote: ... and pXVE::YFP-cPMEI8 plants treated with control MS and 5 µM estradiol for 24 h using the RNeasy Plant Kit (QIAGEN, Hilden, Germany). For RNA isolation from meristematic and elongation zones ...
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bioRxiv - Immunology 2023Quote: ... Tissue RNA was isolated from preserved samples using 30 minutes of automated homogenization at 30 Hz with 5 mm ball-bearings in RLT Plus Lysis Buffer (Qiagen TissueLyser II), RNA was then isolated using a RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... snap-frozen tissue was processed by adding 500 µl of QIAzol reagent in the presence of one 5-mm stainless steel bead (Qiagen, Hilden, Germany). Total RNA isolation from homogenized tissues was performed using a Qiagen RNeasy kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: Mosquitoes were homogenised in pools of ≤ ten in 300 µl tissue culture medium using the Qiagen TissueLyser II with 5 mm stainless steel beads (both Qiagen, Manchester, UK) and centrifuged (10,000 rpm/10 min) ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were run in a total volume of 10 μl having 5 μl Rotor-Gene SYBR® Green PCR Kit (Qiagen, NSW, Australia), 0.3 μl each of 10 μM forward and reverse primer and 2 μl of genomic DNA ...
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bioRxiv - Biochemistry 2021Quote: ... 30 mg of sample were aliquoted in Eppendorf tubes and homogenized in 400 mL of methanol for 5 min at 25 Hz with a sample disruptor Qiagen TissueLyser II Laboratory Mixer (Qiagen, Valencia, CA, USA). The homogenized mixture was centrifuged for 10 minutes at 10000g (4 °C) ...
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bioRxiv - Microbiology 2019Quote: ... punched papers with faeces or bee samples were homogenized by a stainless-bead (5 mm Ø) in a Tissue Lyser II (Qiagen, Hilden, Germany). Total RNA was eluted in 30 μl RNase-free water and quantified by a Qubit RNA HS kit on the Qubit fluorometer (Thermo fisher scientific ...
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bioRxiv - Microbiology 2021Quote: ... Intact bacterial cells were pelleted by centrifugation at 10,000 x g for 5 min at room temp and DNA extracted from the pellet using a DNeasy® Blood & Tissue kit (Qiagen, Hilden, Germany). DNA concentration was assessed by the QubitTM dsDNA BR Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Following the manufacturer’s instructions RNA was reverse-transcribed in a 20 μl reaction volume (42°C, 30 min; 95°C, 5 min) using a QuantiTect Reverse Transcription Kit (Qiagen, Valencia, CA, USA). cDNA was then amplified using a SYBR Green I Master mix (Roche ...
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bioRxiv - Genomics 2021Quote: ... we pooled 1.5 mg RNA from each sample (mixed-stage, male-enriched, and starved) and used the RNeasy MinElute Cleanup kit (Qiagen, catalog no. 74204) to further purify and concentrate the pooled RNA ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 minutes at 300xg and pellets resuspended in Buffer RLT Plus (RNeasy Plus Mini Kit from Qiagen, Germantown, MD, USA), and frozen at −80°C ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were homogenized with a 5-mm steel bead in a TissueLyser bead mill (Qiagen; operated at 50 Hz for 30 seconds). The homogenized samples were incubated at room temperature for 10 minutes for complete cell lysis and then centrifuged at maximum speed for 5 minutes to pellet insoluble material ...
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bioRxiv - Genetics 2024Quote: ... Biopsies no larger than 5 x 10 x 10mm were washed with ice-cold PBS three times and placed in RNAlater™ (Qiagen, Germantown, MD). After a minimum incubation of 24 hours in RNAlater at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extract (1 mg) was incubated with His6-RabD2c (1 μg) and Ni-NTA Agarose (QIAGEN) for 1 h at 4°C ...