Labshake search
Citations for Qiagen :
251 - 300 of 10000+ citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: Successful transfection and affitin expression were checked by Western blotting using a RGS-His6 HRP conjugate (Qiagen) to detect Affi_224 in a HEK-293T cell lysate ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The RBD was purified from the expression media by metal-affinity chromatography using Ni-NTA beads (Qiagen). The Fabs were purified from the expression media using rProtein A Sepharose Fast Flow beads (GE healthcare) ...
-
bioRxiv - Microbiology 2021Quote: ... RORC1 and RORC2 gene expression was evaluated by One step SYBR green real-rime RT-PCR (Qiagen) using a Light-Cycler 480 II as follows ...
-
bioRxiv - Pathology 2022Quote: ... HSP90AB1) and fold changes/regulation of gene expression were calculated using the 2^(−ΔΔCT) formula (GeneGlobe, QIAGEN). Differential expression (up and down regulation ...
-
bioRxiv - Developmental Biology 2022Quote: ... Gene expression of different fos family genes in zebrafish was analyzed using Quantitect™ Primer Assays (Qiagen) for each individual gene – fosaa ...
-
bioRxiv - Cancer Biology 2023Quote: Small interfering RNA was obtained to knockdown the expression of ARSB and galectin-3 (Qiagen, Germantown, MD). Effects on mRNA were determined by QRT-PCR ...
-
bioRxiv - Cancer Biology 2023Quote: ... Differential expression analysis was performed using the RNA Sequencing Data Analysis Workflow from CLC Genomics Workbench (Qiagen). Relative coverage was calculated to the total reads.
-
bioRxiv - Genomics 2019Quote: Differential gene expression data were analyzed using the IPA software suite (https://www.qiagenbioinformatics.com/products/ingenuitypathway-analysis) (QIAGEN Inc). Genes related to liver fibrosis and hepatocellular carcinoma were obtained by searching for the terms “liver fibrosis” and “Hepatocellular carcinoma” in the Diseases and Functions search field ...
-
bioRxiv - Biochemistry 2021Quote: ... supernatant was harvested after 7 days of expression and incubated with 300 μL of Ni-NTA resin (Qiagen) at 4°C overnight ...
-
bioRxiv - Cancer Biology 2020Quote: Quantitative PCR was performed using SYBR Green or TaqMan Gene Expression Assays in a Rotor Q Gene (QIAGEN). The primer/probe sequences are summarized in Suppl ...
-
bioRxiv - Physiology 2022Quote: ... DESeq2 normalized count and differential expression sheets were loaded to GSEA (Broad Institute, 36, 37) or IPA (Qiagen) for interrogation as indicated in the text ...
-
bioRxiv - Microbiology 2021Quote: ... Functional and network analysis of statistically significant gene expression changes was performed using Ingenuity Pathways Analysis (IPA, QIAGEN). Significant canonical pathways are identified from IPA based on enrichment of the zscore.
-
bioRxiv - Bioengineering 2020Quote: ... and CCR2 expressions were determined by real time-PCR using RT2 SYBR Green qPCR Mastermix (Qiagen, Hilden, Germany) on a CFX384 Real-Time PCR Detection System (Bio-Rad Laboratories ...
-
bioRxiv - Plant Biology 2019Quote: ... The protein prokaryotic expression and purification was according to the instruction of Ni-NTA Agarose (Cat.No.30210, QIAGEN) and Glutathione Sepharose (GE).
-
bioRxiv - Cell Biology 2019Quote: ... knockdown of KCNB1 expression in human cells was carried out using a mixture of 4 siRNA duplexes (Qiagen; Cat# ...
-
bioRxiv - Biophysics 2019Quote: ... The SpyTag-(Cpa)4-SpyTag and the HaloTag-SpyCatcher constructs were cloned into the expression plasmid pQE80L (Qiagen). Protein expression and purification was done as described elsewhere36 ...
-
bioRxiv - Physiology 2019Quote: The mRNA expression of 34 metabolic genes was analyzed using a custom RT2 Profiler PCR Array (Qiagen, USA). First ...
-
bioRxiv - Immunology 2020Quote: ... Relative gene expression was calculated using the Qiagen webportal PCR array data analysis web-based software (Qiagen, Germany). Differential expression (up and down regulation ...
-
bioRxiv - Cell Biology 2022Quote: ... Genes with significantly altered expression between PTPN23H/H and PTPN23+/+ IAT were analyzed with Ingenuity Pathway Analysis (Qiagen) to identify impacted pathways and candidate upstream drivers ...
-
bioRxiv - Immunology 2022Quote: ... considering a cutoff log2Fold change in expression in pSS vs HD >1.5 and < −1.5) was performed using Ingenuity Pathway Analysis (Qiagen) and NetworkAnalyst (35 ...
-
bioRxiv - Microbiology 2023Quote: Full-length sdeC genes were amplified by PCR and cloned into the bacterial expression vector pQE-80L (Qiagen) with BamHI and SacI restriction sites to generate a 6xHis-SdeC-StrepII fusion construct (Supplemental Table 1) ...
-
bioRxiv - Cancer Biology 2024Quote: ... mRNA expression levels were measured with the RT2 Profiler PCR Array with a custom designed panel (QIAGEN, 3445261) using the Power SYBR™ Green PCR Master Mix (QIAGEN ...
-
bioRxiv - Cell Biology 2024Quote: ... Gene expression changes associated with osteogenesis were assessed using an RT2 Profiler™ PCR Array (PAMM-026Z, Qiagen). A fold regulation threshold of 2 was used to determine up- or down-regulation of each gene in male and female PAE mice compared to control and data is presented as log2 fold change ...
-
bioRxiv - Cancer Biology 2021Quote: Differential expression of cell cycle-associated genes was determined using the EMT RT2 profiler qPCR array (Qiagen, PAHS-020Z) and analyzed using the Qiagen online Data Analysis Tool ...
-
bioRxiv - Molecular Biology 2019Quote: ... mRNA expression levels were normalized to the housekeeping human TATA-binding protein (TBP) mRNA (RT2 qPCR Primer Assays, Qiagen) in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT ...
-
bioRxiv - Genomics 2019Quote: ... Total RNA and DNA isolation for gene expression microarrays was carried out using the QIAcube system (Qiagen cat.# 9001292) with the AllPrep DNA/RNA Universal Kit (Qiagen cat.# 80224 ...
-
bioRxiv - Neuroscience 2020Quote: ESR1 expression was manipulated with custom-designed locked nucleic acid (LNA™) 15-mer antisense oligonucleotides designed by Qiagen following Kelly & Goodson.41 The antisense oligo for ESR1 knockdown (ESR1-KD ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA expression of 84 apoptotic genes was analyzed using the RT2 Profilμer PCR Human Apoptosis Array (Qiagen, PAHS-012Z). Arrays were prepared according to the manufacturer’s protocols applied to the prepared cDNA samples ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Fold changes of N transcript expression with each treatment were calculated corcenning to the Virus control using REST 2009 program (v2.0.13, Qiagen, GbmH) [26] ...
-
bioRxiv - Physiology 2019Quote: ... Gene expression data was analyzed using nonparametric tests in REST 2009 Software (M. Pfaffl, Technical University Munich, and QIAGEN). A two-way ANOVA was used to analyze torque as well as fatigue resistance after PGC-1α transfection (group by treatment) ...
-
bioRxiv - Microbiology 2021Quote: ... Purified PCR products were digested with KpnI and SphI and ligated into expression vector pQE-80L (Qiagen, Mississauga, ON) digested with same restriction endonucleases ...
-
bioRxiv - Biochemistry 2021Quote: The DNA sequence encoding each ankyrin repeat domain was chemically synthesized and cloned into pQIq expression vectors (Qiagen, Germany) by Gibson Assembly 38.
-
bioRxiv - Bioengineering 2023Quote: ... The expression levels of genes involved in ECM and cell adhesion were analyzed using RT2 Profiler PCR Arrays (Qiagen). Reverse transcription polymerase chain reaction (RT–PCR ...
-
bioRxiv - Developmental Biology 2020Quote: ... PMSG- or hCG-regulated genes in wildtype GCs that showed a differential expression in Erβnull GCs were selected as ERβ-regulated genes and subjected to Ingenuity Pathway Analysis (IPA; Qiagen Bioinformatics) to determine their involvement in folliculogenesis.
-
bioRxiv - Molecular Biology 2021Quote: ... recombinant CLEC16A and CLEC16A ΔC were generated following expression in 293T cells and purified with nickel-charged resin (Ni-NTA agarose; Qiagen) per the manufacturer’s protocols.
-
bioRxiv - Biophysics 2019Quote: ... Clarified lysate from bacterial expression was passed over a column with Streptactin Superflow resin or Streptactin XT Superflow resin (Qiagen). After incubation ...
-
bioRxiv - Cancer Biology 2019Quote: ... Stable cell lines were obtained by co-transfection of each vector with a piggyBac transposase expression vector via Effectene (Qiagen) transfection reagent ...
-
bioRxiv - Biophysics 2021Quote: ... MHC II with C-terminal hexahistidine tags on both α and β chains were expressed using a baculovirus expression system in S2 cells and purified using a Ni–nitrilotriacetic acid (NTA) agarose column (Qiagen). The histidine-tagged MHC bacmid (Malherbe et al. ...
-
bioRxiv - Microbiology 2020Quote: ... The sequences were aligned against the PAO1 reference genome NC_002516 available in the “Pseudomonas Genome Database” (PseudoCAP) [37] and gene expression was quantified using the “CLC Genomics Workbench” software (QIAGEN). The numeric value of gene expression was normalized to Reads Per Kilobase of gene per million Mapped reads (RPKM) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The statistical test applied was the DGE (Differential Gene Expression) using the RNA-seq package with CLC Genomics Workbench© v 20.0 (Qiagen). The expression values for the transcripts were registered in TPM (Transcripts per Million ...
-
bioRxiv - Microbiology 2020Quote: ... Gene expression was assessed using SYBR GreenER based-quantitative polymerase chain reaction (qPCR) or RT2 profiler arrays (Qiagen Ltd, UK). For SYBR GreenER based-qPCR analyses ...
-
bioRxiv - Developmental Biology 2022Quote: To determine relative mRNA expression levels total RNA was collected from RA-treated cells at various time points using QiaShredder (Qiagen) and RNeasy (Qiagen ...
-
bioRxiv - Molecular Biology 2020Quote: Functional annotation of genes that are associated with dynamic chromatin regions and/or differential expression with NSC activation was performed using Ingenuity Pathway Analysis (Qiagen Inc. ...
-
bioRxiv - Microbiology 2022Quote: ... Initial gene expression profiling was performed using the 96-well Human Cytokines & Chemokines RT2 Profiler PCR Array (PAHS-150ZC, Qiagen) according to manufacturer instructions ...
-
bioRxiv - Genetics 2022Quote: ... It uses the same volumes as the standard Visium Spatial Gene Expression protocol but with an addition of 15µl Vapor-Lock (Qiagen, 981611) to each sample during the first incubation (fragmentation ...
-
bioRxiv - Microbiology 2019Quote: ... was used to carry out the gene expression study following the manufacture’s protocol with a Rotor-gene Q Instrument (Qiagen, Australia). Melt curve analyses were performed in the range 55– 90°C to examine reaction specificity of the designed primers ...
-
bioRxiv - Microbiology 2019Quote: ... The resulting 6xHis-tagged expression constructs were utilized to generate recombinant enzymes that were purified via Ni-NTA agarose (Qiagen). IDS enzyme assays were carried out in triplicate as previously described [45] ...
-
bioRxiv - Cell Biology 2020Quote: ... and gene expression was assessed using RT2 Profiler PCR Array Human WNT Signaling Pathway Plus (PAHS-043YC-2, Qiagen Germany). Arrays were run on QuantStudio6 (Thermo Fisher Scientific ...
-
bioRxiv - Pathology 2021Quote: ... and gene expression was carried using the RT² Profiler PCR Array for human cellular stress responses (Qiagen, Catalog#:PAHS-019ZA) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... 10 μl of the gene expression assay (RT2 SYBR Green ROX qPCR Master Mix) (cat. # 330520, Qiagen, Valencia, CA, USA) and 1 μl of the relevant mouse RT2 qPCR Primer Assay ...