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Citations for Qiagen :
251 - 300 of 863 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified by Ni2+-affinity (Ni-NTA agarose, Qiagen) then passed over an anion-exchange column (Hitrap Q HP ...
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bioRxiv - Microbiology 2024Quote: ... soluble proteins were incubated with a Ni-NTA column (Qiagen) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... The His-tagged proteins were purified using Ni-NTA (Qiagen) following the manufacturer’s protocol and eluted using lysis buffer containing 200 mM imidazole ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were incubated with Ni-NTA Agarose (Qiagen, Cat#30230) resin for 30 min at 4 °C in a beaker with stirring ...
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bioRxiv - Microbiology 2024Quote: ... HA proteins were purified using Ni-NTA agarose resin (Qiagen) and gravity flow chromatography columns (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... protein lysate was loaded onto a Ni-NTA column (Qiagen) and eluted with a buffer containing 20 mM Tris–HCl pH 8 ...
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bioRxiv - Genetics 2024Quote: ... Soluble proteins were incubated with nickel-nitriloacetic acid beads (Qiagen) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified with Ni-NTA chromatography (Ni2+-nitrilotriacetate, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the proteins were purified on a Ni-NTA column (Qiagen Inc.), eluted with imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... E2 and polyPRM5 proteins were purified using Ni NTA-agarose (Qiagen) followed by cation exchange (Source15S ...
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bioRxiv - Cancer Biology 2020Quote: ... The bacterial His-tagged proteins were purified under denaturing conditions (Qiagen) and refolded by dialysis as described previously (22) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were immobilized on a glutathione agarose gravity flow column (Qiagen) washed 5 times with PBS solution and eluted in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Plant Biology 2020Quote: GRF5 protein sequences were aligned using CLC DNA Workbench 8.0 (Qiagen) with the following settings ...
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bioRxiv - Immunology 2021Quote: ... The proteins were captured using Ni-NTA (Qiagen, Germantown, MD, USA), washed in EQ buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... Fusion proteins were purified from clarified lysate by Ni-NTA (Qiagen) affinity chromatography ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen) and the histidine tag was subsequently removed by thrombin digestion (10 U/mg of protein) ...
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bioRxiv - Microbiology 2022Quote: ... recombinant protein was initially purified by nickel affinity chromatography (Qiagen, Holland) and subsequent size exclusion using Superdex 200 increase (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins in the supernatants were purified using Ni-NTA beads (Qiagen) according to (62) ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified by FPLC using a Ni-NTA column (Qiagen), PBE-94 column (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were purified using Ni-nitrilotriacetic acid (Ni-NTA; Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA was extracted with DNA/RNA/Protein Mini Kit (Qiagen). The quality and quantity of RNA was assessed using the Agilent RNA 6000 Nano Kit Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Biophysics 2022Quote: ... The protein was purified from culture supernatant with Ni-NTA (Qiagen), biotinylated with in-house purified BirA enzyme ...
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bioRxiv - Genomics 2021Quote: ... The proteins were bound to a Ni-NTA sepharose beads (Qiagen), extensively washed with binding buffer and eluted in buffer containing 50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... and proteins were purified with Ni-NTA resin (Qiagen, Cat# 151010181) and followed by Superose 6 10/300 gel filtration column chromatography (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... The solubilized protein was then purified using Ni-NTA agarose (Qiagen). The purity was confirmed (>98% ...
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bioRxiv - Microbiology 2020Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen), the eluted fractions containing S protein were pooled ...
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bioRxiv - Microbiology 2021Quote: ... cruzi proteins attached to addressable Liquichip Ni-NTA beads (Qiagen, CA) and T ...
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bioRxiv - Molecular Biology 2021Quote: ... The fusion protein was affinity purified using Ni-TNA agarose (Qiagen). Details are provided in the Supplementary Information methods section.
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bioRxiv - Microbiology 2023Quote: ... Protein was purified from sonicated lysates using Ni-NTA resin (Qiagen) and gravity flow ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on 4-12% Bis-Tris gels (Novex, Qiagen) using MOPS running buffer (Novex ...
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bioRxiv - Developmental Biology 2023Quote: ... After the protein digestion with Proteinase K (QIAGEN, Cat. No. #158920) and RNA digestion with RNase A (QIAGEN ...
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bioRxiv - Biochemistry 2023Quote: ... The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Molecular Biology 2022Quote: The His-tagged protein was purified by Ni-NTA agarose (Qiagen). Eluted proteins from beads were then applied to the NGC chromatography system (Bio–Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were purified using Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). To purify JetAB complexes containing one full-length and one truncated JetA protomer ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen), eluted with 50 mM Tris-base ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Ni2+-nitrilotriacetic acid agarose beads (Qiagen) and desalted with Bio-Gel P-6DG gel (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... Protein was purified under native conditions according to the QIAexpressionist (Qiagen) protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen). Isolated DNA and RNA samples were quantified by Nanodrop (ThermoFischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were purified by affinity chromatography using Ni-NTA resin (Qiagen) and a gravity column (Biorad) ...
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bioRxiv - Biophysics 2024Quote: SNAP-GAF protein was purified on Ni-NTA agarose (Qiagen 30210), followed by Amylose resin (NEB cat ...
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bioRxiv - Plant Biology 2024Quote: ... His-FLAG-ATG8 proteins were purified using Ni-NTA agarose (QIAGEN), and MYC-UBR7 and MBP-UBC8 proteins were purified using anti-MYC (Pierce ...
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bioRxiv - Microbiology 2024Quote: Protein thermostability was evaluated with a Rotor-Gene Q instrument (QIAGEN). A fresh 1:200 mix of Sypro Orange dye (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were purified through Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). For bNACHT11 and gp057 ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were purified using a two-column protocol: Ni-NTA (Qiagen) affinity chromatography followed by size exclusion chromatography (HiLoad Superdex 75 pg or 250 pg ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were purified using Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). JetAB and JetC proteins were further purified by anion-exchange chromatography (HiTrap Q HP ...
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bioRxiv - Biochemistry 2024Quote: ... Protein unfolding was monitored with a Rotor-Gene Q instrument (QIAGEN) using the blue channel (excitation ...
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bioRxiv - Cell Biology 2024Quote: ... and the His-tagged protein was purified using Ni-NTA (Qiagen). Similarly ...
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bioRxiv - Developmental Biology 2024Quote: ... and protein was purified using Ni-NTA beads (Qiagen, Cat:30230). Purified proteins were reduced ...
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bioRxiv - Biochemistry 2023Quote: ... The PentaHis antibody (Qiagen, Düsseldorf, DE) against His-Tagged proteins was diluted 1:5000 in TBS buffer containing 3% BSA and the membrane was incubated overnight at 4°C followed by 2×10 min washing steps in TBST and 1×10 min in TBS buffers ...