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Citations for Qiagen :
251 - 300 of 6282 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Twenty-five μl reactions contained 12.5 μl 2X QuantiFast SYBR Green Mix (Qiagen), 1 μM of each primer ...
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bioRxiv - Cell Biology 2022Quote: ... For the real-time PCR 2x QuantiFast SYBR Green PCR Master Mix (Qiagen) was used ...
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bioRxiv - Developmental Biology 2021Quote: ... Every other hour ~300 embryos were counted and lysed in 350μl of a solution of RLT buffer and β-mercaptoethanol from the Qiagen RNeasy Micro Kit (Qiagen, Hilden, Germany). The lysates were immediately stored at −80°C until use ...
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bioRxiv - Bioengineering 2021Quote: ... the mEV solution was incubated with 100 µl of precipitation buffer B from the Qiagen miRCURY Exosome Isolation Kit (Qiagen, Germantown, MD) overnight (∼12 hours ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA removal was performed by applying 80 μl of DNase I working solution (10 μL DNase stock + 70 μL 1x RDD buffer; RNase-Free DNase Set, Qiagen, Hilden, Germany) to the column and incubated for 15 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 200 µl aliquots of the sample solution were processed by either the in-house method or using the DNeasy Blood & Tissue Kit (Qiagen Cat 69504) per manufacturers’ instructions.
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bioRxiv - Genetics 2020Quote: ... Lysis buffer and a sterile 5mm steel bead were added to each sample prior to homogenisation on a TissueLyser LT (Qiagen). DNA was extracted from the homogenised lysates using the MasterPure DNA Purification kit (epicentre ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 and SARS-CoV RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... each sample was from a separate NRVM isolation from a total of 3 isolations) and homogenized in RLT buffer (Qiagen) using green RINO homogenization tubes following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2020Quote: ... The pooled sample was quantified using the KAPA Library Quantification Kit and adjusted to 2-4nM with Buffer EB (QIAGEN) for sequencing on Illumina platforms ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 200 mg of thawed and mixed faecal samples were added to 1 ml of InhibitEX Buffer (QIAGEN, GmbH, Hilden, Germany) followed by the beat-beating step in TissueLyser II (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... 50uL of the dounce homogenized sample was labelled as “input” and added to 350uL RLT buffer with 1% 2-mercaptoethanol (Qiagen), followed by RNA extraction ...
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bioRxiv - Genetics 2023Quote: ... liver samples were homogenized in Qiazol lysis buffer in a TissueLyser II and RNA isolated with RNeasy Mini Kit (Qiagen) following manufacturer’s protocols ...
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bioRxiv - Evolutionary Biology 2023Quote: High-molecular weight DNA was extracted from podetia of Noboribetsu samples using buffers in DNeasy Blood & Tissue Kit and Genomic-tip 20/G (QIAGEN) with lysis time extended to 5 hours ...
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bioRxiv - Neuroscience 2022Quote: SARS-CoV-2 RNA was harvested from the cell medium at various time points post infection and stored in viral lysis buffer with RNA supplements (50 μl of sample medium in 560 μl of AVL buffer supplemented with 1% carrier RNA) (Qiagen). RNA was extracted using QIAamp Viral RNA Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 200 μL of the resuspension buffer as added.The resulting samples were purified using a QIAquick PCR purification kit (Qiagen, Germany).The sequence mixtures were prepared using a USB® Thermo Sequenase Cycle Sequencing Kit (Affymetrix ...
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bioRxiv - Neuroscience 2022Quote: Tissue cultures that were cultivated on one filter membrane (3 cultures) were transferred as one sample into RLT buffer (QIAGEN) and RNA was isolated according to the manufacturer’s instructions (RNeasy Plus Micro Kit ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from frozen tissue samples using ceramic beads (Precellys homogenizer, 1× 20 s) in RLT buffer (RNeasy Mini kit, Qiagen) supplemented with 1%ß-mercaptoethanol ...
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bioRxiv - Neuroscience 2023Quote: ... containing RNA derived from the entire cortical sample was collected and lysed with 350 µl of buffer RLT from Qiagen RNeasy Kit supplemented with 10μl/ml bME ...
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bioRxiv - Microbiology 2024Quote: ... BALF samples and 10% tissue homogenates were mixed with an equal volume of the RLT buffer (Qiagen, Germantown, MD, USA). Two hundreds microliter of the sample lysate and 200 µL isopropanol was added into the extraction plate ...
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bioRxiv - Immunology 2024Quote: ... samples within a cryotube were disrupted using a pestle and homogenized by pipetting with 600 µL of RLT buffer (Qiagen). Homogenized samples were then transferred to a Qiashredder spin column (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 18 samples for each cell line were lysed in RLT buffer from RNeasy mini kit (#74106, Qiagen) and RNAs were extracted following the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... The tissue powder was then transferred to a 1.5 mL centrifuge tube where 400 μL of 1% PVP-40 Buffer AP1 solution and 4 μL of RNase A were added (Qiagen DNeasy Plant Kit Qiagen Inc, Hilden, Germany). The tube was initially vortexed to homogenize the solution before incubation at 65 °C for 30 minutes with a short vortex every 5 minutes.
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bioRxiv - Cancer Biology 2023Quote: ... DNA was eluted with 66 µL of 2 mM Tris-HCl pH=7.0 to carry out Solution A purification or eluted with 66 µL of 0.1 X EB buffer (From QIAGEN Plasmid Plus Midi Kit) without further Solution A purification.
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR was performed using 2X SYBR green quantfast PCR Mix (Qiagen, 1044154) and gene-specific primers as follows ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed 2x with PBS and imaged in FluoroBrite DMEM (Qiagen #A1896702).
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bioRxiv - Biochemistry 2021Quote: ... 700 µL buffer RLT (kit buffer, Qiagen) supplemented with 10 µL/mL β-mercaptoethanol was added to the sample and mixed vigorously before proceeding with the protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Approximately half the volume of each feces sample (100-200 mg) was immersed in 3 ml of Inhibit EX Buffer (QIAGEN, Hilden) and stored at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and swab samples in DMEM were mixed with an equal volume of RLT RNA stabilization/lysis buffer (Qiagen, Germantown, MD, USA), and 200μl of sample lysate was then used for extraction using a magnetic bead-based nucleic acid extraction kit (GeneReach USA ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue scrolls were sectioned from OCT-embedded fresh-frozen samples into Buffer RLT with β-mercaptoethanol (Qiagen Sciences LLC, Germantown, MD). RNA was purified following the protocol from RNeasy Micro Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: Rat epididymal fat and liver tissue samples were lysed in RNA extraction buffer (RLT) from the RNeasy kit (Qiagen, Germantown, MD) with stainless-steel disruption beads for 4 minutes at 30.0 Hz using the Qiagen TissueLyser (Germantown ...
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bioRxiv - Genomics 2020Quote: ... Dehydrated samples were stored at −80 °C until resuspension in 25 μl or 100 μl of Buffer EB (QIAGEN, Venlo, Netherlands).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The sample was then eluted in buffer AE (10 mM Tris-Cl; 0.5 mM EDTA; pH 9.0; Qiagen Inc., Courtaboeuf, France). DNA purity was assessed with a NanoDrop spectrophotometer based on the 260/280 and 260/230 O.D ...
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bioRxiv - Microbiology 2020Quote: DNA extraction was performed by mixing 0.2 g of each feces sample with 500 μL of G2 buffer (QIAGEN, Hilden, Germany) in an Eppendorf tube (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were lysed in RLT buffer supplemented with β-mercaptoethanol and RNA was purified using the RNeasy Mini Kit (#7410, Qiagen) with on-column DNase treatment (#79254 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were thawed and resuspended in freshly prepared RLT buffer (350 μL per sample for 1E6 cells) from the RNAeasy Qiagen kit (Qiagen 74104). Dnase1 digestion buffer was then added and cells were subsequently lysed via passage through a Qiashredder column (Qiagen 79654) ...
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bioRxiv - Bioengineering 2023Quote: ... samples were suspended in RNeasy kit Lysis Buffer and β-mercaptoethanol (1:100 v/v) and homogenized using QIAshredder columns (Qiagen). Samples loaded on spin columns were treated with DNase (Qiagen ...
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bioRxiv - Synthetic Biology 2024Quote: ... and tryptic peptides were prepared by following the established proteomic sample preparation protocol (Chen et al.,. Briefly, cell pellets were resuspended in Qiagen P2 Lysis Buffer (Qiagen, Germany) to promote cell lysis ...
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bioRxiv - Evolutionary Biology 2024Quote: ... with the addition of reservoirs for larger sample volumes12 followed by two rounds of elution with 30 μl elution buffer (Qiagen EB). Extracts were stored at -20°C until library preparation and sequencing at the Genomics Facility of the University of Manchester for data acquisition.
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bioRxiv - Genetics 2021Quote: ... Protein Precipitation Solution (Qiagen) was added at 0.33x and mixed well ...
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bioRxiv - Genetics 2024Quote: ... 1X Q-Solution (Qiagen), 200 µM dNTP (Qiagen) ...
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bioRxiv - Genetics 2024Quote: ... 1X Q-Solution (Qiagen), 200 µM dNTP (Qiagen) ...
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bioRxiv - Cancer Biology 2022Quote: ... The buffers used were: 1x PCR buffer (Qiagen; referred to as Taq buffer); 1x ThermoPol reaction buffer (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... DNA for library preparations and sequencing was extracted using a standard phenol-chloroform extraction from liver and muscle samples which were collected at slaughter and stored in stored in RNAlater® Solution (Ambion/QIAGEN, Valencia, CA, USA).