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Citations for Qiagen :
251 - 300 of 10000+ citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... imidazole (20 mM final) was added to the eluted protein which was subsequently incubated with 5 mL NiNTA resin (Qiagen) at 4 °C overnight with constant inversion ...
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bioRxiv - Biochemistry 2022Quote: Approximately 25 mg of frozen tissues were transferred to 2 mL Eppendorf Protein Lobind tubes containing one 5 mm stainless steel bead (cat# 69989, Qiagen) and 500 µL of lysis buffer consisting of 5% SDS in 50 mM TEAB with protease inhibitors cocktail (cat# A32953 ...
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bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Lysates were incubated for 1 hr at 37°C after adding 10 µl of RNAse A (20 mg/ml) and proteins were precipitated with 200 µl of Protein Precipitation Solution (Qiagen Gentra Puregen Cell kit). After centrifugation ...
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bioRxiv - Cell Biology 2019Quote: ... and stored at −80°C or immediately used for RNA isolation using RNAeasy Universal Mini kit (Qiagen) with on-column DNAse digestion by RNase-free DNase set (Qiagen ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from -80°C stored stool samples using the DNeasy Powersoil HTP 96 kit (Qiagen) and an EpMotion 5075 automated pipetting system (Eppendorf) ...
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bioRxiv - Pathology 2021Quote: ... at 4°C and total RNA was extracted with the RNeasy Midi kit (Qiagen, Santa Clarita, CA). Mouse Genome 430A 2.0 arrays (Affymetrix ...
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bioRxiv - Immunology 2022Quote: ... incubated at 37°C for 35 min and were purified with a MinElute PCR Purification Kit (QIAGEN). After the optimization of PCR cycle number using SYBER Green I Nucleic Acid gel Stain (Takara Bio) ...
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bioRxiv - Systems Biology 2023Quote: ... Cryovials were thawed at 36°C and total RNA was extracted (RNeasy Micro Kit, Qiagen, Hilden, Germany) in one batch ...
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bioRxiv - Physiology 2024Quote: ... Organoids were pelleted and stored at −80° C until RNA extraction using the RNeasy Micro Kit (Qiagen). Reverse-transcription was performed with 850 ng of RNA using the High Capacity cDNA RT kit (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 sections were stored in -80°C prior to RNA extraction using the Rneasy micro kit (Qiagen). RNA quality was assessed using a 5300 Fragment Analyzer (Agilent ...
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bioRxiv - Plant Biology 2020Quote: ... 6 and 48 HAT to ammonium or nitrate amended plates using RNeasy® Plant Mini kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... DNA extractions were performed using a DNeasy PowerSoil 96-well plate DNA extraction kit (Qiagen, Hilden, Germany). The standard protocol was used with the following two exceptions ...
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bioRxiv - Immunology 2022Quote: BMDMs were grown in 6-well plates and RNA was isolated using the RNeasy Mini kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... colonies were scraped off plates and library plasmids purified using a Qiagen HiSpeed Maxi kit (Qiagen, 12662). Plasmids were eluted in Qiagen Buffer TE ...
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bioRxiv - Immunology 2023Quote: ... A second round of PCR was performed to generate uniquely barcoded sequencing libraries using the barcode primer plate included in the kit (17μL of working mix which include 12.5μL QIAGEN 2x Multiplex PCR master mix ...
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bioRxiv - Bioengineering 2023Quote: ... gDNA was purified from cells in 6-well plates using the DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Fibroblasts were grown to confluency in 6-well plates and total RNA extracted using RNeasy Kit (Qiagen). Poly(A)-tailed RNA enrichment and library construction was performed using KAPA stranded mRNA-Seq Kit with KAPA mRNA capture beads (KAPABiosystems) ...
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bioRxiv - Physiology 2019Quote: ... DNA was extracted from 5 µL of RBCs using the Gentra Puregene Tissue Kit (Qiagen) and kept frozen at −80°C until analysis ...
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bioRxiv - Neuroscience 2020Quote: ... Powdered tissue (∼5 mg) was dissolved in lysis buffer provided by RNeasy micro kit (Qiagen), supplemented with 1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from 5 or 6 Transwells using the RNeasy Micro Plus kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... BEAS-2B and MRC-5 (2×106 cells/well) using the RNeasy Mini Kit (Qiagen) with DNase I treatment to eliminate DNA contaminants as previously described18 ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 5 × 106 cells using Qiagen DNeasy Blood & Tissue Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... Digested and 5’ dephosphorylated vector was purified using the QiaQuick PCR Cleanup kit (Qiagen, Germany). Both inserts and vector were eluted and stored in 10 mM Tris-Cl ...
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bioRxiv - Cell Biology 2022Quote: ... 5-6 cryosections were also collected for RNA extraction using RNAeasy Micro kit (Qiagen, 74004). Quality of the RNAs was determined by Tapestation (Agilent ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 5 million cells using RNeasy Plus Universal Kits (Qiagen, #73404) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Cellular RNA of 2-5×105 cells was extracted using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and RNA purified from 5-dpf embryos with the miRNeasy Mini Kit (Qiagen, catalog #: 217004). Using this cDNA library ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was extracted using a modification of the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: ... The extraction was carried out using the AllPrep Bacterial DNA/RNA/Protein Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Protein purification was carried out using QIAExpressionist NiNTA purification kit according manufacturer’s instructions (Qiagen, Germany) and following the HIS purification under native conditions protocol ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from fetal tissues using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen) and bisulfite conversion was carried out using EZ DNA Methylation Kits (Zymo Research) ...
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bioRxiv - Biochemistry 2021Quote: ... Medium was stirred for 1 hour at 4°C in the presence of 5 ml Ni-NTA Agarose beads (Qiagen, Venlo, The Netherlands) washed with 25 mM Hepes-KOH pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... 4A-C and 5A-C were lysed in buffer RLT (Qiagen). The cell solution/suspension? was homogenised using QIAshredder columns (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: RNA was isolated from mEos3.2-lamin A/C WT#21 cells using the RNeasy mini plus kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Reactions were incubated for 16 hours at 16 °C and were terminated by cleaning with miRNeay kit (Qiagen). Crosslink reversal was done by irradiating the RNA on ice 254 nm UVC for 5min using a CL-1000 crosslinker (UVP).
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was incubated for 30 min at 37 °C with mixing (1000 r.p.m.) and directly subjected to DNA purification using the MinElute Reaction Cleanup Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 100ng of RNA was converted to cDNA for 1h at 37 °C using the Omniscript RT kit (Qiagen) and random primers (Promega) ...
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bioRxiv - Immunology 2021Quote: ... Granuloma homogenate was taken for RNA isolation using RNeasy Plus Kits and stored at −80°C (Qiagen, USA). Quantification of viral burden was done using TaqPath 1-step RT-qPCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... the pellet was stored at -80°C before RNA extraction using the Qiagen RNeasy Mini kit (Qiagen, USA), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... for 30 min at 37°C and cleaned up with the RNeasy MiniElute cleanup kit (Qiagen, Hilden, Germany) following the manufacturers’ instructions ...
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bioRxiv - Pathology 2020Quote: ... diffracting crystals of P1_102-157 were obtained at 4°C from condition 7 of the Classic kit (Qiagen) containing 0.1 M tri-Sodium citrate pH 5,6 ...
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bioRxiv - Microbiology 2019Quote: ... at 37°C for 1 hour and cleaned up with the QIAquick PCR Purification Kit (Qiagen, Valencia, CA) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 37 °C) with 50 μg RNA sample and purified using RNeasy Protect Mini Kit (catalog no. 74124, Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24 hours at -20°C prior to DNA/RNA extraction with AllPrep DNA/RNA Mini Kit (Qiagen). Cryopreserved patient ascites cells and PDX cancer spheroids were thawed and rinsed in serum free RPMI before RNA/DNA extraction ...
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bioRxiv - Neuroscience 2022Quote: ... Reaction was incubated at 37 °C for 30 min and purified using the QIAGEN MinElute Kit (28004 QIAGEN) according to the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... Tregs were pelleted and stored at -80°C in RTL lysis buffer (RNeasy micro kit, Qiagen, Hilden, Germany) with β-mercapto-ethanol (1:100) ...
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bioRxiv - Genetics 2022Quote: Liver samples were stored frozen at −80 °C before RNA extraction with the RNeasy kit (QIAgen, Crawley, UK) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... homogenized samples were stored at −20 °C prior to extraction using the DNeasy® PowerSoil® Kit (Qiagen) following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... at -20°C or immediately processed for RNA using the RNeasy Mini Kit as per manufacturer’s protocol (Qiagen). Real-time analyses were performed as previously described (Van Otterloo et al. ...