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Citations for Qiagen :
2851 - 2900 of 10000+ citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: The decellularized Balb/c mouse pancreas scaffolds were washed in PBS and homogenized-lysed for 1 hr in Tissue homozilyser II (Qiagen). The ECM proteins were dissolved in lysis buffer containing Tris HCl 0.06M ...
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bioRxiv - Genetics 2019Quote: ... Crosslinking was then reversed by overnight incubation at 65°C and DNA purified using QIAquick PCR purification column (Qiagen, 28104). Immunoprecipitated DNA was then quantified via Qubit (ThermoFisher ...
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bioRxiv - Physiology 2019Quote: ... 72 °C - 10 min) and amplicons separated using a Qiaxcel Advanced Separation System using 15 - 3000 bp markers (Qiagen, UK).
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bioRxiv - Biochemistry 2021Quote: ... The insoluble fraction was precipitated by ultracentrifugation (20,000 g) for 30 minutes at 4°C and the supernatant was loaded onto a Ni-NTA superflow affinity column (QIAGEN). The Ni-column was then wash three times and eluted with buffer containing 30 mM HEPES (pH 7.8) ...
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bioRxiv - Neuroscience 2020Quote: ... at 60°C overnight and 1 μl of a 1:20 dilution of the lysate used in a PCR reaction (HotStarTaq, Qiagen). The sequence flanking the tmt-opsin1b TALEN binding sites was amplified using the forward 5’-GGGACTTTCTTTGCGCTTTA-3’ and the reverse 5’-CAGGTCAGAGCGGATCTCAT-3’ primers ...
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bioRxiv - Biochemistry 2021Quote: ... the cell lysate was cleared by centrifugation (20000 rpm, 30 minutes, 4 °C) and purified using Ni2+-affinity chromatography (Ni-NTA superflow cartridges, Qiagen). Typically two 5 mL columns were used for a 2 L culture in which the lysate was loaded on the column washed with 10 CV wash buffer (50 mM Hepes ...
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bioRxiv - Biochemistry 2021Quote: ... the cell lysate was cleared by centrifugation (20000 rpm, 30 minutes, 4 °C) and purified using Ni2+-affinity chromatography (Ni-NTA superflow cartridges, Qiagen). Typically two 5 mL columns (flow 5 mL/min ...
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bioRxiv - Bioengineering 2021Quote: ... Exosome samples were treated with 1 µL RNAse A/T1 per 100 µL sample for 30 min at 37°C and lysed in 650 µL of QIAzol (Qiagen). To monitor the RNA recovery percentage as a function of the isolation procedure ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant obtained after high speed centrifugation (20 000 x g for 30 min at 4 °C) was loaded on a gravity nickel-nitrilotriacetic acid (Ni-NTA) metal affinity column (Qiagen), washed with 10 column volumes (CV ...
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bioRxiv - Plant Biology 2021Quote: ... The translation mixture was gently agitated in a microtube for 1 h at 4°C with a fivefold volume of Ni-NTA agarose (Qiagen) that had been equilibrated with a solution containing 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... Samples were incubated for 15 min at 37 °C to methylate accessible chromatin before the reaction was stopped with the addition of RLT plus buffer (Qiagen) and samples frozen down and stored at −80 °C before processing ...
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bioRxiv - Biophysics 2021Quote: ... NF-κB molecules were immobilized through the 6xHis-tag on the C-terminus of RelA by penta-His antibody biotin conjugate (34440, Qiagen). For DNA-bound NF-κB experiments ...
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Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projectionsbioRxiv - Developmental Biology 2022Quote: ... Sorted ßGal+ or GFP+ cells were collected in 1.5ml DNA LoBind® tubes placed at 4°C containing 400µl RLT buffer (Qiagen, 79216) with 1% β-Mercaptoethanol (Millipore Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... The Klenow polymerization reactions were incubated at 37 °C for 1 h and then purified on ion-exchange cartridges (cat. no. 28306; Qiagen). The 194 purified reactions were dissolved in 50 µL of water each and pooled to generate the 3,880 members library (DEL3880 ...
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bioRxiv - Cancer Biology 2022Quote: ... Library plasmid pools were propagated in liquid culture in LB with ampicillin (100 μg/ml) at 30 °C overnight and extracted (Qiagen).
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The samples were powderized with a mortar and pestle under liquid nitrogen or a TissueLyser II chilled to -80°C (Qiagen). The samples were lysed in RIPA buffer for 30 min on ice with intermittent vortexing (0.15M NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... the manufacturer’s directions were followed with the exception of adding 500ul of 10% SDS (w/w) to the homogenate at 60°C for 20 minutes before the addition of P2 buffer (Qiagen). This allowed the brain tissue to better solubilize with the Qiagen buffers ...
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bioRxiv - Microbiology 2020Quote: ... Tissue sections were incubated with proteinase K for 18h at 56°C prior to extraction on the QiaSymphony (Qiagen, USA) following manufacturer recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells incubated for 24 hours in a 37 °C incubator and transfected with hsa-miR-24-3p miRCURY LNA miRNA Power Inhibitor (Qiagen, Germantown ...
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bioRxiv - Microbiology 2019Quote: ... all clinical isolates and the UCBPP-PA14 reference strain were cultivated at 37 °C in LB broth and harvested in RNAprotect (Qiagen) at OD600= 2 ...
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bioRxiv - Immunology 2019Quote: ... Plugs were incubated twice (2 h of incubation followed by an overnight incubation) at 50 °C with 167 μL of freshly prepared proteinase K (Qiagen) in 2.5 mL of lysis buffer (BioNano Genomics Inc. ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was incubated at 30 °C for 90 min before applying it to 50 µl of Ni-NTA agarose resin (Qiagen) pre-equilibrated in W25 buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Lysates were clarified by centrifugation at 24,000gat 4 °C for 20 min and passed through 2 ml of Ni-NTA nickel resin (Qiagen, 30250) pre-equilibrated with wash buffer ...
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bioRxiv - Genomics 2019Quote: ... to avoid fragmentation of long DNA molecules, with overnight lysis at 50°C in lysis buffer (Bionano Genomics, USA) with Puregene Proteinase K (Qiagen). The volume ratio (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 20,000 rpm for 1 hour at 4°C and the soluble fraction was affinity purified by gravity column with Ni-NTA affinity resin (QIAGEN). The protein was eluted by 50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2019Quote: ... Cell lysate was separated by centrifugation at 28000 x g for 45 min and 4°C and the supernatant was loaded onto a column containing 2 mL of Ni-NTA agarose (Qiagen) equilibrated with buffer A for 1 h for purification by nickel affinity chromatography ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris was removed by centrifugation at 20000 g for 40 min at 4 °C and the lysate incubated with Ni-NTA Agarose Beads (Qiagen). After washing ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was clarified by centrifugation at 10,000 × g for 30 mins at 4 °C and the supernatant was applied to an Ni-NTA affinity column according to the manufacturer’s instructions (Qiagen, Germany). Bound proteins were washed twice with wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2019Quote: ... The lysate was centrifuged for 10 min at 16,200xg 4°C and 600 μL of the supernatant was added to a 50 μL slurry of Ni-NTA Agarose (Qiagen) that had been washed three times with 250 μL A3B-CTD lysis buffer without 2-mercaptoethanol and once with 250 μL of A3B-CTD lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... worms were homogenized in PBS (50 μl/adult worm pair or 50 μl/1000 schistosomula) at 4°C using a TissueLyser II (Qiagen), homogenates were incubated overnight with mixing at 4°C and the supernatants collected by centrifugation at 15,000 g for 1 h at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were incubated for 15 minutes at 37°C to methylate accessible chromatin before the reaction was stopped with the addition of RLT plus buffer (Qiagen) and samples frozen down and stored at -80°C prior to processing ...
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bioRxiv - Microbiology 2019Quote: ... an 8 μg of the isolated RNA was further treated with 2U of TURBO DNase (Invitorgen) at 37°C for 1 h and purified with RNeasy spin columns (Qiagen). The amount of RNA was quantified with Qubit RNA BR assay kit (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... This was followed by RNase A treatment for 1 h at 37°C and purification using MinElute PCR purification columns (Qiagen). cDNA amplification was performed with reporter-specific nested cDNA primers (Oligos 16 and 17 for libraries in vectors d and e ...
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bioRxiv - Genomics 2021Quote: ... To eliminate lipids supernatants were applied to a RNeasy column and centrifuged at 10,000 rpm and 4 °C for 1 min (Qiagen, 74104). The flow through was collected and protein concentrations were assessed using the Bradford assay ...
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bioRxiv - Genetics 2020Quote: ... coli strains expressing (His)6-Clr4 and (His)6-Swi6 and allowed to bind at 4°C overnight to Ni-NTA resin (Qiagen; binding capacity 5-10mg protein/ml resin) ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µL of the mixture was then incubated for 45-60 minutes at room temperature or 4°C with 10 µL NiNTA resin (Qiagen) prewashed with His-SUMO Lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified extract was supplemented with 10 mM imidazole and rotated for 2 hours at 4 °C with 1 ml of Ni-NTA beads (Qiagen). Bound protein was eluted with 10 CV of buffer L / 200 mM imidazole ...
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bioRxiv - Developmental Biology 2021Quote: ... Confirmation and segregation of the HNRNPUL1 c.1406dup variant was performed by PCR amplification (HotStar Taq Plus, Qiagen, Toronto, ON) from genomic DNA and Sanger sequencing with the ABI BigDye Terminator Cycle Sequencing Kit v1.1 (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... and incubated at 37°C for 45 min after which 25 µl of proteinase K (20 mg/mL) and 200 µl Buffer AL (Qiagen) was added ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were clarified by centrifugation at 24,000g at 4°C for 20 min and passed through 2 mL of Ni-NTA nickel resin (Qiagen, 30250) pre-equilibrated with wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Genetics 2019Quote: ... Lysates were clarified by centrifugation at 12,000xg at 4°C and mixed with 200 µL of a slurry of Ni2+-NTA agarose (Qiagen #30210) that had been equilibrated in lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... All plugs were incubated twice (2 hours incubation followed by an overnight incubation) at 50 °C with freshly prepared 167 μl Proteinase K (Qiagen) in 2.5 ml lysis buffer (BioNano Genomics ...
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bioRxiv - Microbiology 2022Quote: ... DNA on the exterior of filtered capsids was digested for 1 hour at 25 °C with 20.3 units DNase (Qiagen: 79254) supplemented with 10 mM MgCl2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cleaved fragments were released by incubating the tube for 30□min at 37□°C and then purified by Qiagen MinElute PCR Purification Kit ...
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bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease73 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
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bioRxiv - Plant Biology 2022Quote: ... the supernatant was discarded before resuspending the nuclei pellet in 30 mL prewarmed (37 C) G2 lysis buffer (Qiagen, Germany). Resuspended pellets were incubated with RNase A (50 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... A melting curve determination experiment from 20 to 95 °C was then performed on the reaction on a Rotor-GenQ qPCR machine (QIAgen) with 563 excitation filter and 610 high-pass emission filter (gain:10) ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were induced with 100 μM IPTG at 12°C for 16 hrs and purified on a Ni2+ column according to manufacturer protocols (Qiagen). Proteins were eluted using 125 mM imidazole ...
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bioRxiv - Synthetic Biology 2022Quote: ... were set up on ice avoiding bubbles and incubated at 37 °C for about 25 min in a PCR cycler Rotor-Gene Q (Qiagen, excitation at 470 nm ...
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bioRxiv - Physiology 2022Quote: ... They were subsequently organized in metal brackets that were stored at -20°C and homogenized with stainless steel beads at 30 Hz using a TissueLyser II (Qiagen). Samples were lysed twice for one minute ...