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Citations for Qiagen :
2801 - 2850 of 3900 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The resultant washed DNA (100 μl) was then cleaned with a DNeasy PowerClean Pro Cleanup Kit (Qiagen 12997-50).
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bioRxiv - Microbiology 2019Quote: ... Total genomic DNA was extracted from tissues (50-130 mg) using the Qiagen DNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The DNA pellet was washed with 70% (v/v) ethanol and resuspended in 50 μL nuclease free water (Qiagen). DNA concentration and purity were determined by agarose gel electrophoresis and fluorometrically using RiboGreen (Qubit Assay Kit ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from 50 mg tissue or about 1.106 cells using QIAzol Lysis Reagent (#79306, QIAGEN, France). Briefly ...
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bioRxiv - Microbiology 2022Quote: Whole-genome DNA of Muribaculaceae strain MF13079 was extracted with QIAamp BiOstic Bacteremia DNA Kit (12240-50, Qiagen, USA) and sequenced with the Oxford@ Nanopore PromethION platform (performed by Nextomics Biosciences ...
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bioRxiv - Microbiology 2019Quote: DNA was extracted from 50-150mg fecal material using the Qiagen PowerSoil DNA Isolation Kit (Qiagen, Venlo, The Netherlands) with the following protocol modifications ...
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bioRxiv - Developmental Biology 2019Quote: We collected three batches of 50 embryos and isolated total RNA from each using the RNeasy MicroKit (QIAGEN, #74004), in which a needle and syringe and a QIAshredder (QIAGEN ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM EDTA pH 8.0 at 50 ºC for 30 min followed by centrifugation then purification by QIA-PCR clean up (Qiagen). The 193 bp DNA from each round was amplified by KOD then Taq polymerase and purified by anion exchange chromatography using a MonoQ column (48 ...
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bioRxiv - Genomics 2021Quote: ... Amplified libraries were then purified using 1.5x ratio of SPRI beads to remove adapter dimers and eluted in 50 μl of EB (Qiagen) after an incubation for 10 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Shotgun libraries were prepared from 50 ng genomic DNA using the QIAseq FX DNA Library Kit (Qiagen, Germantown, MD), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 μg RNA were further purified by performing three times the clean-up procedure with the RNeasy kit (QIAGEN). DNA digestion was carried out as before between each clean-up step ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Total RNAs were isolated from 50 mg of tongue tissues with RNeasy total RNA isolation kit (Qiagen, Venlo, Netherland) as described in the manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... 25mg of tissue was dissociated using Bead Ruptor Elite (Omni International) for 45 seconds at 4.85m/s and RNA was isolated using RNeasy Fibrous Tissue Mini Kit 50 (Qiagen) following manufacturer’s indications ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μg of gDNA were digested with KpnI and passed through a QIAGEN Genomic-tip 20/G column (QIAGEN) to enrich for replication intermediates ...
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bioRxiv - Genomics 2022Quote: ... 50 μl of neuronal suspension containing about 7,500 nuclei was used for RNA extraction using RNeasy Mini kit (QIAGEN) and RNase-Free DNase Set (79254 ...
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bioRxiv - Immunology 2023Quote: CD8+ T cells from PBMCs and colon were FACS sorted directly into 50 μL of RLT Lysis Buffer (QIAGEN) supplemented with 1% v/v 2-mercaptoethanol ...
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bioRxiv - Pathology 2023Quote: RNA was extracted from frozen adipose tissue pieces (50-100 mg) with the RNeasy Lipid tissue Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: The total RNA was isolated from 50-100 mg of cells using RNeasy Plant Mini kit (Qiagen, Düsseldorf, Germany) and treated with DNA-Free kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from 50 mg of fresh biomass using the DNeasy UltraClean Microbial Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50% processed for RNA isolation using the RNEasy Midi Kit with optional on-column DNAse digestion (Qiagen, 75144), as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1 g yeast extract in 750 ml of 30 kDa filtered seawater and 250 ml of Milli-Q water) using the DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... and the cycle-threshold CT translated to bacterial load (estimated CFU per mL (eCFU/mL) using a standard curve on a Rotor gene Q 5plex platform (Qiagen). The cut-off for TB-MBLA positivity is a 30 CT value that corresponds to 1.0 log10 eCFU/mL ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmids were transformed into JM109 chemically competent cells and grown overnight in 250 mL of LB broth with 100 µg/mL of ampicillin and purified using the HiSpeed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Immunology 2020Quote: ... Supernatants were removed and the remaining cell pellet (500 μl) was transferred into a 15 ml tube containing 2.5 ml RNAprotect® Cell Reagent (Qiagen). The cells were resuspended by vortexing ...
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bioRxiv - Immunology 2020Quote: ... in 96 flat bottom plates in the presence or absence of PMA (200ng/ml) and ionomycin (300ng/ml) were lysed in 100Sµl lysis buffer containing TCL buffer (Qiagen, 1031576) containing 1% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... fungal DNA was extracted independently from 1 mL of crude conidial suspensions (between 108 and 109 spores mL-1) using the DNeasy 96 Plant Kit (Qiagen) following the manufacturer’s instructions (Gautier et al. ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from 3 months old Pgrcre and FOXL2OE diestrus uteri using RNeasy mini kit (Qiagen). The library was prepared using TruSeq RNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... beat-beading tubes were filled 1/3 with beads and soaked overnight in 500 μl buffer RLT (Qiagen). Tubes were spun at full speed and excess buffer removed ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from 3-day-old seedlings using a QIAshredder and RNeasy Plus Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 1000 µL PBS was added to each sample (lungs, 0.01–0.04 g) along with Tungsten carbide 3 mm beads (Qiagen). Samples were homogenized at a speed of 10 Hz for 10 min and then centrifuged at 15,000 × g for 10 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were then ground to powder using a 3 mm tungsten carbide bead (Qiagen Cat. No. / ID: 69997) on a TissueLyser II (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... homogenized in sterile 0.05 % NP40 in H2O for 3 minutes at 25 Hz using a Tissue Lyzer (Qiagen) and serial dilutions were plated on YPD agar containing 100 μg/ml Ampicillin ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were transfected at 3 DIV with siRNA targeting Kdm6a or ntRNA using Effectene Transfection Reagent (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 500 µl of PBS-AB was added to each sample along with Tungsten carbide 3 mm beads (Qiagen). Samples were homogenized during 10 min and then centrifuged at 15,000 g for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed 3 times with PBS and lifted with 500µL/well of Cell Protect Reagent (Qiagen) for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... benthamiana leaves were grinded in a tube with two glass beads (3 mm) with a TissueLyser II (Qiagen) and directly after supplied with 600 μl EB ...
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bioRxiv - Microbiology 2019Quote: ... RNA samples from 3 independent cultures for each strain (Chr_dam and Chr_gfp) were extracted with RNeasy miniprep kit (Qiagen). Primers used are listed in Table S1 ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from an aliquot of 3 x 106 cells using the RNeasy Plus Kit (Qiagen). cDNA was synthesized from extracted RNA using the iScript cDNA Synthesis Kit (BioRad ...
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bioRxiv - Genomics 2024Quote: ... with two 3 mm metal beads and ground into a fine powder with a TissueLyser II (Qiagen, Germany) at 30 Hz for 2 min ...
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bioRxiv - Cell Biology 2023Quote: siRNA targeting human GPRC5A (siGPRC5A_4 against target sequence 5′-CTGGGTGTGTTGGGCATCTTT-3′, cat# SI04438021) and non-targeting control siRNA (cat# Ctrl_AllStars_1, target sequence not disclosed) were purchased from Qiagen. Human primary keratinocytes were transfected at 20 nM final siRNA concentration using Lipofectamin 2000 reagent (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was isolated from HEK293T cells 3 days after transfections using the Gentra Puregene Cell Kit (Qiagen) and was diluted to 10 ng/μL ...
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bioRxiv - Molecular Biology 2022Quote: ... washed 3 times with PBS and had their genomic DNA extracted with the QIAamp DNA mini kit (QIAGEN). Sequencing libraries were prepared at GenomeScan (Netherlands ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was isolated from 0 or 3% CSE-treated bronchospheres using the Qiagen RNeasy Mini Kit (Qiagen 74106). The RNA yield was measured using Agilent TapeStation (4200 TapeStation System ...
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bioRxiv - Microbiology 2023Quote: ... Individual mosquitoes were homogenized for 3 minutes at a 30Hz/s frequency in a TissueLyser II grinder (Qiagen). DNA extraction of individual larvae was carried out using the QIAamp DNA Kit (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was extracted from 3-week-old plants using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: RT-qPCR was performed in an Applied Biosystems QuantStudio 3 using the QuantiTect® Multiplex PCR Kit (QIAGEN). The primers and probes used are listed in Table S2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we amplified samples (N=3 of each species) using the PyroMark PCR kit (Catalog #978703, Qiagen, Germantown, MD) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from 3×106 SYAT or CGR8 cells with the Rneasy Plus Mini Kit (Qiagen, #74134). Reverse transcription was performed on 1 µg RNA using oligodT (Thermo Fisher Scientific #SO131 ...
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bioRxiv - Genetics 2020Quote: ... 1 µl 1 mg/ml Carrier RNA (QIAGEN), 1 µl 10% SDS ...
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bioRxiv - Molecular Biology 2020Quote: ... and then with 0.1 mg/mL RNaseA (Qiagen) for 2h at 37°C ...