Labshake search
Citations for Qiagen :
2751 - 2800 of 10000+ citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: RNA was extracted from blood or biopsies following manufacturer’s protocol using the PAXgene kit and Qiagen Rneasy Mini kit (Qiagen), respectively ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from primary mouse hepatocytes and Huh-7 cells using the RNeasy Plus Micro Kit and cDNA synthesis performed using the QuantiTect Reverse Transcription Kit according to the manufacturer’s protocol (Qiagen). Reactions were performed in triplicate in 384-well plate format ...
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CD4+ T cells regulate sickness-induced anorexia and fat wasting during a chronic parasitic infectionbioRxiv - Immunology 2022Quote: ... DNA from the blood or tissue of mice was extracted using the same kit (Qiagen blood and tissue kit) and qPCR was done using the same primers ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA removed and RNA extracted using a commercial kit (RNeasy Plus Mini Kit, Cat. 74134, Qiagen, Hilden, Germany). Sample processing for quality control and sequencing was completed by the Australian Genome Research Facility (Victoria ...
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bioRxiv - Bioengineering 2023Quote: Total RNA and cDNA were synthesized from the samples using RNeasy mini kit and QuantiTect Reverse Transcription Kit according to the manufacturer’s instructions (Qiagen). qPCR was performed in a Biorad Thermocycler with a reaction volume of 20 µl which constitutes primers (0.5 µM) ...
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bioRxiv - Cancer Biology 2023Quote: ... was isolated using Midi or Maxi kits for the validation screens gDNA was isolated using Midi kits according to the manufacturer’s protocol (Qiagen). PCR and sequencing were performed as previously described.81 ...
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bioRxiv - Microbiology 2023Quote: ... Three nucleic acid extraction kits designed for DNA and RNA extraction were compared: DNeasy PowerWater kit (QIAGEN; Hilden, Germany), NucleoSpin Soil ...
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bioRxiv - Microbiology 2024Quote: ... and RNA was extracted following the manufacturers instructions (Zymo Quick-RNATM Viral Kit or QIAGEN RNeasy Mini Kit, respectively).
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bioRxiv - Genomics 2020Quote: ... 1 mM EDTA and 1% freshly added Proteinase K (Qiagen)) was added to the cell suspension ...
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bioRxiv - Evolutionary Biology 2021Quote: ... One million cells per well were seeded in 6 well plates in 2 mL growth medium the day before transfection and transfected with PolyFect (Qiagen, Düsseldorf, DE) using the manufacturers protocol (changing the medium to growth medium without hygromycin B and puromycin prior to transfection) ...
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bioRxiv - Microbiology 2021Quote: ... ethanol-stored flies were rehydrated in water and transferred to 1.5 ml well plates for homogenisation using a bead beater (Qiagen Tissue Lyzer II). Protein was digested using Proteinase K ...
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bioRxiv - Microbiology 2020Quote: ... bearing a C-terminal histidine tag (ACRO Biosystems, Newark, NJ) was coated at 2 μg/ml on a Ni-NTA plate (Qiagen, Valencia, CA). After washing and blocking ...
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bioRxiv - Developmental Biology 2024Quote: ... collected into 96-well plates (on ice) prior to the addition of 10 μL of RLT plus lysis buffer (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% v/v ethanol ...
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MK2 deficiency decreases mortality during the inflammatory phase after myocardial infarction in micebioRxiv - Physiology 2023Quote: ... First strand cDNA was synthesized from 0.5 mg of total RNA using QIAGEN RT2 First Strand Kits and transcripts for mouse cytokines and chemokines were quantified using qPCR microarrays comprising 96-well plates precoated with primers (QIAGEN PAMM-150Z). Each 96-well plate also contained primers for 5 housekeeping genes as well as positive and negative controls ...
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bioRxiv - Cell Biology 2019Quote: ... CLASP-1 (Qiagen) – GCCAUUAUGCCAACUAUCUdTdT (Mimori-Kiyosue et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Kid-1 (Qiagen) – CAAGCUCACUCGCCUAUUGTT (Wandke et al. ...
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bioRxiv - Cell Biology 2021Quote: ... DNase 1 (Qiagen) was used to remove contaminating DNA followed by a second cleanup ...
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bioRxiv - Developmental Biology 2022Quote: ... SPACA6 concentrated to 7 mg mL−1 in Buffer B was mixed in a 1:1 volumetric ratio (0.3:0.3 mL) with JCSG+ (Qiagen), Cryos (Qiagen) ...
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bioRxiv - Immunology 2021Quote: The ipsilateral hemispheres were lysed in Qiazol Lysis Reagent and total RNA was extracted using the MaXtract High Density kit with further purification using the RNeasy Mini Kit (all Qiagen). 70 ng of total RNA per sample was then hybridized with reporter and capture probes for nCounter Gene Expression code sets (Mouse Neuroinflammation codeset ...
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bioRxiv - Genetics 2021Quote: ... Cells were lysed with Precellys CKMix Tissue Homogenizing Kit (Bertin Technologies) and total RNA was extracted using Qiagen RNeasy Maxi Kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from lung homogenates according to the instructions of the RNA extraction kit manufacturer (RNeasy Plus Mini Kit; Qiagen) post phase separation using Trizol reagent ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from lung homogenates according to the instructions of the RNA extraction kit manufacturer (RNeasy Plus Mini Kit; Qiagen) post phase separation using Trizol reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA extraction was done using RNeasy Plus Mini Kit following Proteinase K digestion and elimination of DNases using RNase-free DNase kit (all reagents from Qiagen). Isolated RNA was then converted to cDNA using Bio-Rad iScript kit and RT-qPCR using Bio-Rad SYBR Sso Advanced ...
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bioRxiv - Genetics 2019Quote: ... gDNA was extracted from dried blood spots and whole-genome amplified using QIAmp DNA Mini kits and Replica-G Midi amplification kits (Qiagen), respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA and RNA were extracted from sections of the frozen tissue using the QIAamp DNA Mini Kit and RNeasy Mini Kit (Qiagen), respectively ...
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bioRxiv - Genetics 2020Quote: ... Reverse transcription was performed using bead-bound RNA (SuperScript VILO cDNA synthesis kit) and qPCR was performed in triplicate (QuantiFast SYBR Green PCR Kit; Qiagen) on a Roche LightCycler 480 using beta-actin as reference gene ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA extraction and cDNA synthesis were performed the following day using QIAamp RNA Blood Mini Kit and cDNA Synthesis Kit respectively (Qiagen), and then stored at −80°C until assayed ...
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bioRxiv - Microbiology 2020Quote: ... RNA extraction was performed using the QIAamp 96 DNA kit and the Qiacube HT kit and the Qiacube HT (both from Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... RNA was removed using NaOH hydrolysis and cDNA was purified using the buffers from the QIAquick Nucleotide Removal Kit and the columns from the MinElute Reaction Cleanup Kit (Qiagen). Finally ...
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bioRxiv - Genomics 2019Quote: Genomic DNA was extracted from the caudal fin of each challenged fish using the DNeasy Blood & Kit tissue kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... DNA fragments were extracted from agarose gels using the QIAquick gel extraction kit and from liquids using the QIAquick PCR purification kit (QIAGEN). Plasmid DNA was isolated using the Wizard Plus SV Minipreps DNA purification kit (Promega ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... We extracted total genomic DNA from blood and/or tissue samples using standard nucleic acid extraction kits (QIAamp DNA Mini Kit; Qiagen) automated on a QiaCube (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: Brain samples were lysed in Qiazol Lysis Reagent and total RNA was extracted using the MaXtract High Density kit with further purification using the RNeasy Mini Kit (all Qiagen). 70 ng of total RNA per sample was then hybridized with reporter and capture probes for nCounter Gene Expression code sets (Mouse Neuroinflammation codeset ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from ∼100 mg of root tissue and DNase treated using the RNeasy plant mini kit and RNase free DNase kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... sections of small intestine from the lorikeets were deparaffinized and the DNA extracted using a commercial kit (QIAamp DNA FFPE tissue kit; Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... Genomic DNA was extracted 72 hrs post-transfection using a DNA extraction kit (QIAamp DNA Mini kit; Qiagen, Hilden, Germany). This was subjected to a T7E1 assay following the manufacturer’s instructions (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: Total DNA was extracted from frozen fecal pellets using the PowerSoil-htp 96 well DNA isolation Kit (MoBio) or the DNeasy PowerSoil HTP 96 Kit (Qiagen). Barcoded primes were used to amplify the V3-V4 region of the 16S rRNA gene from extracted bacterial DNA using primers 515fB and 806rB via PCR (EMP) ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was extracted from the same tumor region as the single-cell analyses using the Qiagen AllPrep kit and matched normal blood using DNeasy kit (Qiagen). Briefly ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed with RLT buffer and total RNA was isolated using an RNA purification kit (RNeasy Mini Kit, Qiagen). cDNAs were transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Genomics 2022Quote: DNA was extracted using either the Nanobind Tissue Big DNA kit (Circulomics, USA) or DNeasy Blood & Tissue Kit (Qiagen, Germany) by following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Samples were homogenized using a motorized pestle and total RNA was extracted and purified using a RNeasy Plant Mini Kit and RNeasy MinElute Cleanup Kit (Qiagen). RNA was sent to GENEWIZ ...
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bioRxiv - Microbiology 2022Quote: ... Cytochrome b products were excised and purified from the gel using a commercial kit (QIAquick Gel Extraction Kit, Qiagen, Germany), followed by purification of eluted DNA using AMPure XP Magnetic Beads (1X ...
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bioRxiv - Genetics 2022Quote: ... These cells were lysed with Precellys CKMix Tissue Homogenizing Kit (Bertin Technologies) and total RNA was extracted using Qiagen RNeasy Maxi Kit (Qiagen). mRNA was isolated with Oligo (dT)25 Dynabeads (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... according to manufacturer’s protocol (MiScript Primer assays and II RT kit for cDNA synthesis and MiScript SYBR Green PCR Kit for RT-qPCR, 218161, Qiagen) from which the recovery of cel-miR-39 spike-in control was confirmed.
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bioRxiv - Neuroscience 2020Quote: mRNA isolation and reverse transcription reaction were performed respectively with RNeasy Mini kit and with QuantiTect Reverse Transcription kit (both from Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from Ca9-22 cells after 4 or 5 hours of culture using the RNeasy Mini prep Kit and cDNA was prepared using the RT2 First Strand Kit (Qiagen) or the qScript™ cDNA Synthesis Kit (Quanta Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: gDNA extraction for genotyping was performed on snap-frozen culture-derived cells using the DNeasy Blood & Tissue Kit and the QIAmp DNA Micro Kit from Qiagen, according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA extraction was performed using the QIAamp 96 DNA kit and the Qiacube HT kit and the Qiacube HT (both from Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... All DNA purifications were performed using a Roche High Pure PCR product purification kit or a QIAquick Gel Extraction Kit (Qiagen). Standard procedures for DNA digestion and ligation were used in conditions recommended by the enzyme manufacturer (Promega or Fermentas) ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid and genomic DNA isolations were carried out with the QIAprep Spin Miniprep Kit and the DNeasy Blood & Tissue Kit (Qiagen), respectively.