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Citations for Qiagen :
2701 - 2750 of 3031 citations for 6 1 Aminoethyl 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: Total RNA yields from the PBMC and stimulated PBMCs were in the range of 3 micrograms (50-100 ng/μl in 30μl Qiagen EB buffer) determined by absorbance at 260nm on the NanoDropTM One (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from aerial parts of 3-week-old seedlings grown on soil using either RNeasy Plant Mini Kit (Qiagen, 74904) or Monarch Total RNA Miniprep Kit (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... RT-PCR products were gel purified in 3% agarose gel and cleaned up using QIAGen gel extraction kit (QiaGen, Hilden, Germany).
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bioRxiv - Cell Biology 2021Quote: ... The plasmid DNA was extracted from 3-9 positive colonies where possible after blue-white screening using a QIAprep® Spin Miniprep Kit (Qiagen) and sequenced using the standard T7 primer at Eurofins Genomics ...
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bioRxiv - Cancer Biology 2022Quote: ... and GSC6-27 HEPACAM shRNA (n=3) cultures were washed in cold 1X PBS and total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, 74134) following manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2022Quote: ... Trimming of the obtained sequencing reads and mapping to the pea aphid genome (version 3) were performed with the program CLC Genomics Workbench (Qiagen Bioinformatics) (mapping parameter ...
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bioRxiv - Bioengineering 2022Quote: ... A cell mass of an equivalent of a OD600 3 was used for a DNA mini preparation using a QIAprep® Spin Miniprep Kit (Qiagen). DNA was eluted in 50 μL elution buffer and measured using a NanoDrop one (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... neurons were transfected by lipofection at 3 days in vitro (DIV) with target siRNA or ntRNA using Effectene Transfection Reagent (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... or a non-targeting scrambled control (Scr; sequence: 5’-TAACACGTCTATACGCCCA-3; Qiagen, Cat No.: 339137; 0.1 nmol in 2 μL PBS), using a 2 μL Hamilton syringe at a rate of 1 μL/min ...
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bioRxiv - Bioengineering 2022Quote: ... RNA extraction was carried out at day 3 of culture on polyacrylamide substrates using an RNeasy Plus Micro Kit (Qiagen, 74034). This time point (different to the 6 day time point used in immunocytochemistry experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... medium was removed and cells were lysed on the plate by addition of 3 ml of lysis reagent RLT Plus (Lot 169010027, Qiagen, Germany). 1 ml of lysate was transferred to a 2 ml Eppendorf tube ...
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bioRxiv - Synthetic Biology 2023Quote: ... Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with LZRS-vector constructs using Effectene transfection reagent (Qiagen, 301425) as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... MiRs (61 plus 3 controls) were quantified on a custom PCR plate using the miRCURY LNA miR SYBR Green PCR Handbook (Qiagen, Germany). PCR (40 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of plasmids pBS-5’UTR-POI-HiBit-3’UTR were linearised by restriction enzyme digest and purified using QIAGEN QIAquick PCR Purification Kit (Qiagen, 28104) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Total RNA of the supernatants collected from SARS-CoV-2 infected Calu-3 cells 48 h post-infection were isolated using the QIAamp Viral RNA Mini Kit (Qiagen; #52906) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... DNA extraction was done by firstly washing samples with 5 mL of filtered autoclaved seawater 3 times before resuspending in 200 µl of lysis buffer from the MagAttract HMW DNA Kit (Qiagen, USA) with 20 µL of 10 µg/mL proteinase K and incubated for 1 h at 56℃ ...
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bioRxiv - Microbiology 2020Quote: ... Fresh frozen tissue homogenates were prepared by thawing frozen tissue and placing 200 mg (± 50 mg) of minced tissue in a tube containing 1 ml DMEM culture medium and a steel bead (Qiagen, Germantown, MD, USA). Homogenization was performed with the TissueLyser LT (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of the template was used in a 25 μL reaction using the QuantiFast Probe RT-PCR Kit (Qiagen, Inc., Valencia, CA) with 0.4 μM of each primer and 0.2 μM probe ...
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bioRxiv - Molecular Biology 2019Quote: ... Residual inactivation beads were removed by spinning the RNA sample through a QIAshredder column at 1000 g for 1 min (Qiagen-catalogue no. 79654). 2μg of total RNA input was retained for each sample and 30μg was incubated for 1.5 h at RT with 100 μg of Biotin-HPDP (Pierce - catalogue no ...
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bioRxiv - Genetics 2021Quote: Gene Ontology (GO) enrichment and network analysis of RNAseq results were performed using both Ingenuity Pathway Analysis (IPA) software package (Qiagen version 1-16) and manually developed excel pipeline workflows ...
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bioRxiv - Developmental Biology 2021Quote: ... Lower jaws were resuspended in 600 μl of RTL plus buffer supplemented with 1% β-mercaptoethanol (M3148-100ML, MilliporeSigma, Burlington, MA, USA) and Reagent DX (19088, Qiagen, Hilden, Germany). HH31 and HH34 lower jaws were processed in a Bead Mill 24 Homogenizer (15-340-163 ...
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bioRxiv - Developmental Biology 2020Quote: ... Lower jaws were resuspended in 600 μl of RTL plus buffer supplemented with 1% β-mercaptoethanol (M3148-100ML, MilliporeSigma, Burlington, MA, USA) and Reagent DX (19088, Qiagen, Hilden, Germany). HH31 and HH34 lower jaws were processed in a Bead Mill 24 Homogenizer (15-340-163 ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR products were checked on 1% agarose gels before being purified using a QiaQuick gel extraction kit (Qiagen, Inc., Valencia, California, USA) and directly sequenced in both directions using the amplification primers on an ABI 3730 automated sequencer (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from the supernatant of Vero E6 cells after 1 passage using the QIAamp Viral RNA Mini kit (Qiagen, https://www.qiagen.com/us/) without addition of carrier RNA ...
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bioRxiv - Genetics 2019Quote: ... DNA was isolated from 1 g of unexpanded leaves with CTAB method and purified with Genomic Tip 20/G (Qiagen K. K. Tokyo). The extracted DNA was sheared into 20 kb fragments using g-TUBE (Covaris ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then stained by Coomassie-blue or immunodetected as described before [26] using primary polyclonal antibodies directed against His6 epitope-tag (Penta His, Qiagen, dilution 1:1000), V5 epitope-tag (Bethyl Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl, QIAGEN, >600 mAU / ml) and left at 55 °C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Lung homogenates were prepared by homogenizing 200 mg of tissue in a tube containing 1 mL DMEM and a steel bead with a TissueLyser LT (Qiagen, Germantown, MD, USA) for 30 s at 30 hertz ...
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bioRxiv - Neuroscience 2022Quote: PON2 level was quantified in the mouse whole brain homogenate in 100 mg per 1 ml of QIAzol® lysis reagent (Qiagen, Hilden, Germany). mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc. ...
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bioRxiv - Microbiology 2021Quote: ... approximately 1×106 Jurkat cells were washed twice in PBS and total DNA was extracted with the Qiamp® DNA Minikit (Qiagen, Manchester, UK). Quantitative PCR reactions were carried out as previously described using 0.3pmol each primer and 0.15 pmol of the probe in 25 μL volume containing 100-300ng total DNA using an ABI Prism® 7000 Sequence Detection System (SDS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 1 μg of total RNA was used for cDNA conversion using the RT2 first strand kit (Cat# 330404, Qiagen, Valencia, CA, USA). The prepared cDNA was diluted and used to determine the expression of genes of interest using specific primers ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 female and/or 1 male per litter) were homogenised and total RNA was extracted using RNeasy Mini Kits (liver; Qiagen, Chadstone, VIC, Australia) or Trizol reagent (muscle and adipose tissue ...
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bioRxiv - Systems Biology 2019Quote: ... Approximately ∼50 mg frozen tissue was pulverised in methanol-chloroform (300 µl, 2:1 v/v) using a TissueLyser (Qiagen, West Sussex, UK). Then the mixture was sonicated for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA from untreated or RSL3-treated (with or without 2 hr pre-treatment with 2 µM Ferrostatin-1) MM1S and MM1R cells was extracted using the RNeasy Mini Kit (Qiagen, Venlo, the Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: Genomic DNA (gDNA) was extracted from 1-month-old Arabidopsis thaliana rosette leaves with the DNeasy plant mini kit (Qiagen, cat. no. 69104) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and 1:10 dilution of cDNA was used to quantify genes using SYBR Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen, USA). ADP-Ribosylation Factor 1 (AB050957.1 ...
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bioRxiv - Pathology 2024Quote: A sample of ca 1 mm3 excised from the middle section of left lateral liver lobe was cryo-homogenized using TissueLyser II (QIAGEN, Germantown, MD, USA), resuspended ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time quantitative PCR was performed on 40 pg cDNA using specific primers (Supplementary Table 1) and miRCURY® LNA® miRNA SYBR® Green PCR (Qiagen). Values were normalized to miRNA quantity and expressed as relative expression to control WS using formulae 2−ΔCT.
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were washed once with 1×PBS after which DNA was isolated using the QIAamp DNA Mini Kit (Qiagen, Venlo, The Netherlands) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from GFP-empty vector-transfected and GFP-Δ(1-240) JPh1-transfected C2C12 myoblasts using the RNeasy plus mini kit (Qiagen, Hilden, Germany), based on manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were weighted using an analytical scale and homogenized in 1 ml of deionized water using TissueRuptor II electric homogenizer by Qiagen (Germantown, MA, USA). RO5256390 was extracted using acetonitrile/water 50%/50% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The DNA was separated on a 1% agarose gel and the appropriate band was excised and isolated using a gel extraction kit (Qiagen, Valencia, CA, USA). In-Fusion HD enzyme premix was added to the linearized PCR product and transformed into the Stellar™ Competent Cells (Takara ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We verified the PCR products by 1% agarose electrophoresis with EtBr staining and purified using the QiaQuick PCR Purification Kit (Qiagen, Hilden, Germany, 28104) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from ∼100,000 MSNs from 4 independent differentiations per experimental group according to the manufacturer’s protocol and purified using RNeasy columns (Qiagen). A total of 72 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems ...
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bioRxiv - Genetics 2019Quote: ... cells (from 4 × 104 to 2.3 × 106) were pelleted at 400xg for 4 min and resuspended in 700 μL RLT buffer (Qiagen) with beta-mercaptoethanol (1:100) ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was bound to Ni-NTA Agarose resin (Qiagen), washed with 10 CV of wash buffer (50 mM HEPES-KOH pH=7.5 ...
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bioRxiv - Genetics 2019Quote: Single cells meant for RNA processing were sorted into 96-well full-skirted Eppendorf plates that were pre-chilled at 4°C and were prefilled with 10μL of lysis buffer consisting of TCL buffer (Qiagen) supplemented with 1% beta-mercaptoethanol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... n=4 females [hybrid cross] and n=5 females [conspecific cross]) using the AllPrep RNA/DNA Mini Kit (Qiagen), and was stored at −80°C until sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared using a JA-20 rotor (18,000 rpm for 30 min at 4°C) and incubated with Ni-NTA superflow resin (Qiagen) for 30 min ...