Labshake search
Citations for Qiagen :
2651 - 2700 of 4402 citations for 6H 1 3 Thiazin 6 one 2 ethoxy 4 hydroxy 5 nitro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 5 million cells per cell line using the Qiagen AllPrep DNA/RNA extraction kits (Qiagen, 80004). Nucleic acid quantification and quality detection were performed and RNA sequencing libraries were generated via Illumina Stranded mRNA prep ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA from 5-20 mg of melanoma primary and metastatic tissues was extracted using AllPrep DNA/RNA Mini isolation kit (Qiagen). A total of 100 ng of total RNA was used as input for RNA- seq libraries using SMARTer Stranded Total RNA-seq - Pico input (Takara Bio USA ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from bacterial cells using the RNeasy mini kit (5 mL culture) and RNeasy maxi kit (50 mL culture) (Qiagen). RNA concentrations were measured using a Nanodrop spectrometer (ThermoScientific) ...
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bioRxiv - Synthetic Biology 2023Quote: The pARP3 plasmid for methylation analysis was extracted from 200 mL of an overnight culture of Tol 5 transformant cells using the HiSpeed Plasmid Midi kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Pools of 5 embryos were used for RNA extraction via the Qiagen RNeasy Micro Kit (Qiagen, Valencia CA; Cat# 74004). RNA was eluted in RNase-free water and sent to the New York University Genome Technology Center for RNA-sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Then this amplified product was labeled with biotin at 5’ end followed by purification using the Qiaquick nucleotide removal kit (QIAGEN).To check the drug-protein interaction ...
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bioRxiv - Immunology 2023Quote: Aliquots of ∼500,000 isolated B cells were pelleted at 400g for 5 minutes and then resuspended in 350uL RLT Plus buffer (Qiagen). Total RNA was then isolated using RNeasy Mini Plus kit (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA Extraction and Purification: Genomic DNA was extracted from 5 M cells using the Gentra Puregene Cell Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... MRC-5/hTERT or A549 cells were reverse transfected with ON-TARGETplus siRNA SMARTpools (Horizon Discovery) or FlexiTube siRNA (Qiagen) using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... DNA for metagenomes was extracted from 5 and 0.2 μm filters (47 mm diameter) using the DNAeasy Power Soil Pro kit (Qiagen, Sweden). DNA from viral fraction (142 mm diameter membranes ...
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bioRxiv - Microbiology 2023Quote: ... These tissues were then weighed and homogenized by either Omni homogenizer (Omni International, Omni Tissue Homogenizer (TH) 115V) or by 5-mm stainless steel beads with a TissueLyser (Qiagen) and used for onward analysis of infectious virus and vRNA quantification ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed and incubated in Proteinase K (Roth) and Ribonuclease A (Roth; 5 min, RT) before isolating cellular and viral DNA with DNeasy Blood & Tissue Kit (Qiagen). qPCR was performed using FastStart Universal Probe Master (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA extraction from all fractions was performed using Qiazol at 65°C for 5 minutes with regular mixing and then purified with miRNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... and precipitated with ammonium sulfate as in Niu et al.49.The precipitate was dissolved in 30 ml buffer C and loaded onto a 5 ml column of Ni-NTA-agarose (Qiagen) pre-equilibrated in buffer C ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were incubated with 100 µL DTT (100 mM) at RT for 5 min and recovered by the RNeasy clean up kit (Qiagen) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was resolved by 1.5 % agarose followed by excision and isolation within the size range of 300-600 bp using Gel Extraction Kit (Qiagen). NGS libraries were pooled and quantified by qPCR using NEBNext Library Quant Kit (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... After this step the samples were adjusted to RT for 5 minutes and processed using the RNeasy Lipid Tissue Mini Kit (Qiagen) as described in the user manual ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from pooled triplicates 5 hpi with RNeasy Mini columns according to the manufacturers instructions (RNeasy Kits, Qiagen) and strand-specific qPCRs were performed as described previously (38) ...
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bioRxiv - Cancer Biology 2023Quote: ... The material stored in Qiazol was heated at 65°C for 5 min and RNA was extracted using the RNeasy Lipid Tissue Kit including the optional DNase digestion (Qiagen). RNA quality and concentration were assessed using a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL pre-equilibrated Ni-NTA resin (Qiagen) in a 50 mL falcon tube ...
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bioRxiv - Genomics 2022Quote: ... We incubated the reaction for 5 minutes at 55 °C in a G-Storm GS1 (G-Storm) thermal cycler and after incubation added 5 μl Buffer PB (19066, QIAGEN) to inactivate and strip the transposase from the tagmented DNA ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and 1x antibiotic-antimycotic by a TissueLyser II bead mill with 5-mm stainless steel beads (Qiagen). After brief centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... The sample was stored with RNAprotect following the manufacturer protocol (Protocol 5 from RNAprotect Bacteria Reagent Handbook 01/2020, Qiagen) at -80°C until processed ...
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bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... and added to appropriate volumes of buffer RLT and homogenised at 50 Hz for 5 minutes using the TissueLyser LT (Qiagen), until all tissue was homogenised ...
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bioRxiv - Immunology 2023Quote: ... 5×104 cells were resuspended in buffer RLT supplemented with ý-mercaptoethanol and processed with an RNeasy Micro Kit (Qiagen) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Molecular Biology 2023Quote: ... treated with either the Pin-point system or SpCas9 was extracted 5 days post electroporation using the Gentra Puregene Cell Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: DNA was extracted from fecal pellets (100 mg, n=5 per group) by the QIAmp Power Fecal DNA kit (12850-50, Qiagen). The V4 region of 16S rRNA gene was amplified ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was clarified by centrifugation at 5 °C at 18500 rpm for 30 min and loaded to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB3_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 5 °C at 18 500 rpm for 30 min and loaded to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB2_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the bacterial strains grown in VL55 medium with 5 mM pyruvate using the Qiagen Genomic-tip 100/G DNA isolation kit and associated DNA buffers (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from Day 11 populations (n=20, 5/treatment) using Qiagen DNeasy Blood and Tissue Kit (Qiagen, Germany) adapted from the manufacturer’s instructions to include a lysostaphin lysis step(50) ...
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bioRxiv - Cancer Biology 2023Quote: ... we FACS-sorted single nuclei in 384-well plates prefilled with 5 μL of Vapor-Lock (Qiagen, cat. no. 981611) per well ...
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bioRxiv - Cancer Biology 2023Quote: ... of 5 million AMO1 shIRE1 cells previously treated per condition was isolated using the AllPrep DNA/RNA Mini Kit (Qiagen). gDNA was quantified using Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 5 mM DTT) and incubated for 45 minutes at 65°C before addition of 40 μg Proteinase K (Qiagen) and further incubation for 1.5 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: RNA was isolated from 5×106 sorted WSU-FSCCL IgG+ and IgM+ cells using the RNeasy mini kit (Qiagen, 74004). RNA quality control was performed using a Bioanalyzer (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was thoroughly aspirated and replaced with 20 µl of 1x PBS containing 5 mg/ml of RNase A (Qiagen) and 12 mM of purified H6-mNeonGreen-NLS protein as a loading control ...
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bioRxiv - Immunology 2024Quote: Samples were double-sorted using a FACSAria or FACS Astrios: 600-1000 cells from each population were collected into 5 μl TCL buffer (Qiagen) containing 1% beta-mercaptoethanol (β-ME ...
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bioRxiv - Genomics 2024Quote: Total RNA was isolated from 5 × 106 cells harvested from frozen patient vials using the RNeasy Plus mini-Kit (Qiagen). Libraries were prepared with the NEBNext® rRNA Depletion Kit and were sequenced using the Illumina NextSeq500 platform ...
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Macrophage-specific NF-kappa B activation dynamics can segregate inflammatory bowel disease patientsbioRxiv - Immunology 2019Quote: ... they were lysed in 50μL sterile PBS by high-speed shaking (2 × 2min at 30Hz; TissueLyser II (QIAGEN)) ...
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bioRxiv - Cancer Biology 2019Quote: ... 2 mL of plasma were thawed and cfDNA extracted using the QIAamp® Circulating Nucleic Acid Kit (Qiagen) according to the manufacturer’s protocol and stored at −20°C until use ...
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bioRxiv - Neuroscience 2020Quote: ... The 205bp amplicon from PCR 2 was gel extracted using the MinElute gel extraction kit (Qiagen Cat#: 28606), and sent to Genewiz for Amplicon-EZ targeted deep sequencing and Sanger sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR #2 products were validated using gel electrophoresis and purified using the QIAquick Gel extraction kit (QIAGEN). The purified libraries were then quantified by Nanodrop and Qubit prior to sequencing on an Illumina MiSeq.
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bioRxiv - Systems Biology 2021Quote: ... Differences between samples and controls were calculated based on the 2−ΔΔCT method using RNU6 control primer (Qiagen) as normalizer ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from 2-week-old Arabidopsis seedlings using an RNeasy Plant Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... single cell directly into 2 µl lysis buffer (30 mM Tris-HCl [pH = 8.0], 10 mM NaCl, 0.2 µL Proteinase K [Qiagen, #19133] ...
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bioRxiv - Microbiology 2019Quote: HeLa cells (2×106) were transfected with 100 pmol of validated siRNAs against all human ZDHHCs [42] (Qiagen) using interferrin transfection reagent (Polyplus) ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... The soluble fraction was split between two columns each with 2 ml Ni-NTA agarose beads (Qiagen, 30210) pre-equilibrated with Lysis Buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... The concentrated SPACA6-containing media was applied onto a 2-mL column of Ni-NTA agarose resin (Qiagen). The Ni-NTA resin was washed with 10 column volumes (CV ...