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Citations for Qiagen :
2601 - 2650 of 4373 citations for 3 4 Hydroxy 5 isopropyl 6 oxo 1 6 dihydro pyrimidin 2 ylsulfanyl propionic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: DNA was extracted from whole fecal pellets or 50 µL of in vitro cultures with a DNeasy PowerSoil HTP 96 kit (Qiagen 12955-4) or a DNeasy UltraClean Microbial Kit (Qiagen 10196-4) ...
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bioRxiv - Genomics 2024Quote: ... each using three 4 mm punches, using the GenTegra Complete DNA Recovery Kit (GenTegra, Pleasanton, CA, USA) and the QIAamp Micro Kit (Qiagen, Hilden, Germany), based on Additional File 2 Protocol GQ from Ghantous et al. ...
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bioRxiv - Plant Biology 2020Quote: Quantitative real-time PCR was performed in a volume of 5 mL QuantiTect Probe PCR Kit (Qiagen GmbH, Hilden, Germany) kit and an ABI 7900HT fast real-time PCR system (ThermoFisher Scientific Inc. ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was collected and subjected to affinity purification using 5 mL Hi-Trap column containing Ni-NTA resin (Qiagen) on an AKTA Pure 25L protein purification system (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... and cDNA products containing the R2R adapter attached to their 5′ end were cleaned-up by using a MinElute column (Qiagen) to remove unused primer ...
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bioRxiv - Genetics 2021Quote: Pools of 5 mites were ground to powder in liquid nitrogen and total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: Cells were transfected at 60-80% confluence with 5 nM/1nM (MIN6, EndoCβ-H1, respectively) control or miR-125b mimics (Qiagen) or 50 nM of a mixture of four ONTARGETplus siRNAs against mouse Smad2 ...
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bioRxiv - Biochemistry 2021Quote: ... The RNA was prepared from 5 OD equivalents of stressed and unstressed cells using the RNeasy Plus RNA Isolation Kit (Qiagen). 500 ng RNA of the total isolated RNA were used as a template for the synthesis of cDNA using Oligo(dT ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then aspirated using a freshly flame-pulled patch pipette (2.5 inner diameter) and placed into a 5 μl of lysis Buffer TCL (Qiagen, 1031576) + 1% 2-mercaptoethanol (Millipore-Sigma ...
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bioRxiv - Cancer Biology 2020Quote: DNA and RNA were extracted from the cervical cancer tissues (5-10 mg) using the AllPrep DNA/RNA Micro Kit (QIAGEN) as described by the manufacturer ...
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bioRxiv - Bioengineering 2020Quote: ... 100 ng equivalent of pooled total RNA (5 slices per animal; 7 rats) was used to synthesize cDNA using an RT First Strand Kit (Qiagen). Pre-validated primers targeting rat CXCR4 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The entire flies were mechanically crushed for 30 s at 25 Hz using a 5-mm stainless steel bead in a TissueLyser (Qiagen). Three hundred μL of ACL solution and 20 μL of 16 g.L-1 proteinase K were then added to the samples ...
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bioRxiv - Plant Biology 2020Quote: ... Approximately 250 plants showing the long root phenotype of the revertant were selected at 5 DAS and pooled for DNA extraction using the DNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... followed by flow sorting with a Sony SH800Z (gating on calcein NVOC fluorescence levels) into individual wells of a 96-well plate containing 5 μL of Buffer RLT (Qiagen) and 1% β-mercaptoethanol.
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bioRxiv - Cancer Biology 2021Quote: Driver mutations were derived from.15 CopyNumbers were calculated using the CNVKit package.16 RNA was isolated from cell cultures (+/− 5 μM AGI-5198) using the RNeasy kit (Qiagen). We performed paired-end sequencing of 2×100 with the Illumina Novaseq platform to obtain 8-10 GB per sample ...
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bioRxiv - Neuroscience 2020Quote: ... 10 to 20 mg of frozen tissue was dissociated using 400 μl of RLT Plus in a 2mL extraction tube containing a 5 mm diameter beads (Qiagen) and agitated at 30 HZ for twice 2 minutes in the TissueLyser II (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: RNA was extracted from third-instar larval CNS or adult heads (5-7 days post-pupation) with RNeasy Plus Micro kit (Qiagen). RNA isolation was followed with DNase digestion with Turbo DNA-free (Ambion) ...
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bioRxiv - Microbiology 2021Quote: Bacterial cultures (5 to10 mL) from mid-exponential phase (OD600 = 0.5-0.6) were harvested and treated with RNAprotect reagent (Qiagen, Germantown, MD), and the cell pellet ...
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bioRxiv - Cell Biology 2021Quote: Isolated Tert+ and Tert-cells (≤ 5 cells) were subjected to synthesize the complementary DNA (cDNA) using REPLI-g WTA Single Cell Kit (QIAGEN) and analyzed for gene expression by qRT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... mexicana Cas9 T7 procyclic promastigotes were transfected either with whole PCR reactions or 5 µL of DNA purified using the QIAquick PCR Purification Kit (Qiagen). 8 x 106 log phase cells were prepared by spinning down (1,000 x g for 10 min) ...
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bioRxiv - Plant Biology 2020Quote: ... All other tissues were homogenized in a SPEX CertiPrep 2010-230 Geno/Grinder (Cat No.: 12605297, Fischer Scientific) using 5 mm steel beads (Cat No.: 69989, Qiagen); tubes were shaken in 20 sec bursts at 1500 rpm ...
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bioRxiv - Plant Biology 2021Quote: RNA was isolated from adult (5-week-old) WT and er/erl1/erl2 plants using a Plant RNeasy kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples from the GSK-3484862 and 5-azacytidine treated assays had RNA and DNA isolated simultaneously using the AllPrep DNA/RNA kit (Qiagen), whereas only RNA was isolated from decitabine samples by using the RNAzol total RNA protocol (Sigma) ...
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bioRxiv - Plant Biology 2022Quote: Grains were homogenized using mortar and pestle with liquid nitrogen while other tissues were homogenized in SPEX CertiPrep 2010-230 Geno/Grinder (Cat No.: 12605297, Fischer Scientific) using 5 mm steel beads (Cat No.: 69989, Qiagen). For grain samples ...
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bioRxiv - Plant Biology 2022Quote: RNA was extracted from 5-day-old vertically grown Arabidopsis thaliana seedlings root tissue using RNeasy Mini Kit (Qiagen, www.qiagen.com) with on-column DNA digestion to remove residual genomic DNA using RNase-free DNase according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... to produce 75-base pair single-end reads with aimed mean sequencing depth of >5 M reads per sample as recommended by the manufacturer (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... Blood was collected by cardiac puncture and harvested organs were homogenized in 500 μl PBS with two 5 mm stainless steel beads using a tissue lyser (Tissue-Lyser II, Qiagen) for two 2-minute rounds at 30 Hz and centrifuged to pellet debris for 10 minutes at 8,000 rpm ...
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bioRxiv - Microbiology 2021Quote: ... placed in 250 μL PBS containing a 5 mm stainless steel bead and homogenized using a tissue lyser (Tissue-Lyser II, Qiagen) for 2-minutes at 30 Hz ...
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bioRxiv - Immunology 2020Quote: ... Equal volumes of barcoded PCR2 products (5 μL each) were pooled and PCR column purified using QIAquick PCR Purification Kit (Qiagen). Libraries were quantified using KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA was extracted from 5-day-old wild-type and Mpatg mutant thalli using the RNeasy Plant Mini Kit (Qiagen) and used as a template for reverse transcription using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... PCR triplicates of each cDNA (5 µL) were analyzed in a qPCR on a Rotor-Gene Q (Qiagen, Hilden, Germany) in a total reaction volume of 20 µL ...
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bioRxiv - Microbiology 2020Quote: ... and a portion of the tissue (0.08-0.3g) was placed into pre-labeled microcentrifuge tubes containing 5 mm stainless steel beads (Qiagen Inc., CA). Lung samples were homogenized with DMEM + 2% FBS in a TissueLyser (Qiagen Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... imidazole (20 mM final) was added to the eluted protein which was subsequently incubated with 5 mL NiNTA resin (Qiagen) at 4 °C overnight with constant inversion ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR mixtures were assembled using 50 ng or 5 µl total RNA and QuantiTect Probe RT-PCR Kit (Qiagen). Amplification and analysis were performed using QuantStudio3 system (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... Total RNA (5 μl) was used for cDNA synthesis and qPCR was performed in one step using QuantiTect Probe RT-PCR (Qiagen) on a StepOnePlus System (Applied Biosystems) ...
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bioRxiv - Genetics 2022Quote: ... The gRNA was amplified with oligos containing a 5’ T7 promoter sequence and purified using the QIAquick PCR purification Kit (Qiagen). RNA was generated using the Hiscribe™ T7 Quick High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Microbiology 2022Quote: ... from the top A horizon were immediately collected for DNA extractions in 15 mL tubes containing 5 mL Lifeguard Soil Preservation Solution (QIAGEN) using chilled soil-processing trays ...
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells were eliminated by incubation for 10 min on ice with 5 mL of erythrocyte lysis buffer (Qiagen). Human cells were enriched using the Mouse Cell Depletion Kit and the QuadroMACS magnetic cell separator (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Clonal naïve GMP and neutrophil total RNA was extracted from cells at 5×106 cells/ml using the Qiagen RNeasy Plus Mini Kit (Qiagen) with DNaseI treatment (RNase free DNase Set ...
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bioRxiv - Immunology 2020Quote: ... Within these gates CD8+MR1-5-OP-RU- and CD8+MR1-5-OP-RU+ T cell populations were sorted directly into lysis buffer for RNA isolation (RNeasy Plus Micro kit, Qiagen). Purity of ungated sorted CD8+humMR1-5-OP-RU+ cells was between 91-99% (Fig ...
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bioRxiv - Plant Biology 2021Quote: ... Approximately 250 plants showing the long root phenotype of the revertant were selected at 5 DAS and pooled for DNA extraction using the DNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Pathology 2021Quote: ... 24 hours after HCort treatment all medium was removed using the Bluewasher (BlueCatBio) and cells were lysed for 5 minutes using RLT plus buffer (Qiagen), snap frozen on dry-ice and stored at −80 °C ...
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bioRxiv - Pathology 2021Quote: Striped dolphin brain and lung samples were submitted to total RNA extraction using the TissueLyser II (QI-AGEN) and highspeed shaking in Eppendorf tubes with stainless steel beads (5 mm diameter, QIAGEN). Homogenates were centrifuged at 14,000 rpm for 3 min to remove tissue debris ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted from a pooled sample of 5 individuals using a DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany). Extracted DNA was randomly fragmented to a size of 350bp then end-polished ...
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bioRxiv - Microbiology 2020Quote: ... To the clarified lysate (generally about 50 mg/mL protein content as measured with absorbance at 280 nm) was then added 5 mL of pre-washed (lysis buffer) Ni-NTA resin slurry (Qiagen). The suspension was mixed on an orbital shaker for 60 – 90 minutes at 4 °C and then transferred to a glass column (BIO-RAD EconoColumn) ...
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bioRxiv - Microbiology 2021Quote: ... total environmental DNA was extracted from approximately 5 g of sediment subsampled from inner part of WRC using DNeasy PowerMax Soil Kit (Qiagen) with a minor modification 79 ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from a pooled sample of 5 individuals using a DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany). Extracted DNA was randomly fragmented to a size of 350bp then end-polished ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was collected from multiple stages of zebrafish development (30%-epiboly, 9 somite, Prim-5, High-pec and Larvae) using the miRNeasy kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... PCR amplicons of exon 5 of the mouse Nprl3 gene were purified using a PCR purifications Kit (Qiagen, Hilden, Germany) and sent to the Massachusetts General Hospital Center for Computational and Integrative Biology DNA Core for whole amplicon next-generation sequencing (NGS ...