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Citations for Qiagen :
2601 - 2650 of 2825 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Digoxigenin (DIG)-labelled probes were denatured at 90°C for 4 min and diluted with 1 x microRNA ISH buffer (Qiagen), following protocol by Endisha & Kapoor ...
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bioRxiv - Neuroscience 2024Quote: Total RNA from isolated astrocytes or newborn neurons and MSP-1 differentiation induced astrocytes was isolated using RNeasy Mini Kit (Qiagen).1ng (isolated astrocytes and newborn neurons ...
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bioRxiv - Genomics 2024Quote: ... Cells were briefly vortexed for 1 minute and homogenized by loading the lysate into a QIAshredder spin column (QIAGEN, #79656) and centrifuging for 2 minutes at full speed ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 ml of phenol:chloroform:isoamyl alcohol (25:24:1) was added and samples were bead beaten using a TissueLyser LT (Qiagen, Hilden, Germany) for 10 minutes at 25 Hz ...
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bioRxiv - Systems Biology 2024Quote: ... 1 ml pipette tip connected via a rubber hose to a benchtop air pressure valve with 1 ml of lysis buffer AP1 (Qiagen). Following this ...
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bioRxiv - Immunology 2024Quote: ... Illumina sequencing-compatible Combinatorial Dual Index (CDI) adapters were ligated onto the pools using the QIAseq 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation and sequenced on Illumina MiSeq platform to generate 2×250bp paired-end reads.
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bioRxiv - Immunology 2024Quote: Bladders were weighed and homogenized in 1 mL of sterile PBS at 4°C using a handheld rotor-stator tissue homogenizer (TissueRuptor II, Qiagen). Homogenates were serially diluted ...
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bioRxiv - Immunology 2024Quote: Total RNA was isolated from FACs-sorted PFA-fixed cells (populations as shown in Supplementary Fig. 1) using the miRNAeasy FFPE kit for microRNA extraction (Qiagen). Total RNA integrity was assessed using the Agilent Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: Cell-free HIV-1 RNA was isolated from 50 μl plasma using a QIAamp Viral RNA Mini Kit (QIAGEN, 52906) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was then incubated with proteinase K (600 mAU/ml, diluted 1:60 in RNase-free water; Qiagen, Germany) to digest protein ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified HmlΔ promoter and digested ELAV-GeneSwitch plasmid were each run on a 1% (w/v) agarose TAE gel at 80 V for 1 hour and gel purified using the QIAquick Gel Extraction Kit (QIAGEN). Ligation of the insert and backbone was undertaken at 4°C for 16 hours followed by room temperature (22°C ...
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bioRxiv - Developmental Biology 2024Quote: ... The membrane was then hybridized with 50 pmol mL−1 Biotin-labeled Locked Nucleic Acid-modified DNA probes (designed and synthesized by Qiagen) specific to the 5’ end of tRNA-ValCAC and 5S rRNA ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from 1 ml of this culture using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany), adhering to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and the samples from the Democratic Republic of Congo (n = 1) were processed using the QIAsymphony DSP Virus/Pathogen Kit (QIAGEN) with modifications ...
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bioRxiv - Cancer Biology 2024Quote: 1×106 cells were washed with ice-cold PBS and total RNA was extracted using RNeasy kit (Qiagen, Cat#74106). 1 µg RNA was used for the reverse transcription using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from root tissue 1 cm above the root tip using the RNeasy plant mini kit (Qiagen). Extracted RNA was DNase treated and converted into cDNA with LunaScript RT SuperMix kit (New England Biolabs) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from input and IP samples by incubation with 1 mL Trizol at room temperature for 5 minutes and then RNeasy Lipid Tissue Mini Kit (Qiagen) via the manufacturer’s protocol with on-column DNase treatment (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... the suspension was diluted 4 times and incubated for 1 hour at 4°C with 200 µL of Ni-NTA agarose (Qiagen) preequilibrated in the lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Nanobody library DNA fragments were digested with the restriction enzymes FseI and Nsil at 37°C for 1 hour and purified using gel extraction (Qiagen). Similarly ...
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bioRxiv - Cancer Biology 2024Quote: ... we isolated genomic DNA from 2x106 cells (ensuring an average of 1 000x representation for each gRNA) with the AllPrep DNA/RNA Mini Kit (Qiagen), followed by PCR amplification of the gRNAs using forward (5’-CGATACAAGGCTGTTAGAGAGATA-3’ ...
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bioRxiv - Cancer Biology 2024Quote: Approximately 0.1 g of mouse fecal pellets were homogenized and processed for DNA extraction using the PowerSoil DNA Isolation Kit (Qiagen) with the QIACube automated platform ...
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Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexesbioRxiv - Biophysics 2024Quote: ... using 600 units of enzyme in 1 ml total volume at 37 °C for 16-24 hours and purified using QIAquick PCR purification columns (QIAGEN). For ligation ...
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bioRxiv - Cell Biology 2024Quote: For optimisation of pericyte growth conditions RNA was collected from pericytes seeded at 1×105 in a 12-well plate using the RNeasy Mini Kit (74104, Qiagen) after 24 h in the appropriate GA medium ...
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bioRxiv - Cancer Biology 2024Quote: ... and NT5E KO (EV-, D32N-, G34R-, S37F-transfected) HEC-1-A cells was performed using QIAshredder and RNAeasy Mini Kits (QIAGEN) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The spheres were washed twice and stained with 1:500 PI solution (Bio-Sciences) supplemented with 250 μg/mL RNAse A (Qiagen) and Hoechst nuclear counterstain (2 μg/mL ...
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Plasma Exosomes in Insulin Resistant Obesity Exacerbate Progression of Triple Negative Breast CancerbioRxiv - Cancer Biology 2024Quote: ... 1 μg of RNA was used to prepare 20 μL of cDNA using the QuantiTect Reverse Transcription Kit (Qiagen, 205313). The Mouse RT2 Profiler™ PCR Array for Epithelial to Mesenchymal Transition (EMT ...
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bioRxiv - Genetics 2024Quote: ... 10 µM of primers and diluted DNA (1:50 dilution in nuclease free water) from input and immunoprecipitated (IP) fraction using Rotor-Gene Q machines (Qiagen). The qPCR program details and the primers used for qPCR validation are given below:
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bioRxiv - Genetics 2024Quote: ... RNA was isolated from cultures incubated or not with 1 µM staurosporine for 6 hours to induce apoptosis using the RNeasy Mini Kit (QIAGEN). RNA samples were checked for quality on Agilent 4200 TapeStation System (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR products were analysed by gel electrophoresis using 1% agarose gel and purified using QIAquick gel extraction kit (Qiagen, Hilden, Germany). The DNA concentrations of the gel-purified samples were quantified with Nanodrop OneC (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Amplicons were electrophoresed in 1% agarose gel to check for amplification and the size distribution of the library and then column purified (Qiagen, 28104). Once purified ...
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bioRxiv - Microbiology 2021Quote: ... Each gene segment was amplified using segment-specific primers and extracted from 1% gel slices using Qiagen Gel Extraction Kit (Qiagen, Germany). Purified gene segments were cloned into the plasmid pHWSccdB as previously done (27) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg total RNA was isolated from P0 Hnf4a mutant and control kidneys using the RNeasy Plus Micro kit (Qiagen 74034) followed by mRNA was isolation with NEBNext Poly(A ...
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bioRxiv - Plant Biology 2020Quote: ... Aliquots of RNA (1 µg) from each sample were reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The amplified products were analyzed onto 1% agarose gel (low EEO) and extracted and purified from the gel using the QIAprep Miniprep Kit (Qiagen, Netherlands). The 16S rRNA sequencing reactions were performed using 785F (5’-GGATTAGATACCCTGGTA-3’ ...
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bioRxiv - Immunology 2021Quote: ... 30mg of whole spleen RNA was extract via 30 second homogenization in RLT lysis buffer containing 1% β-mercaptoethanol per RNeasy kit (Qiagen). RNA was reverse transcribed into cDNA using iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from ∼1 x 107 cells using a modified version of the Gentra Puregene Cell Kit cell extraction protocol (Qiagen 158745). Integrity of genomic DNA and absence of contaminating RNA was confirmed via 1% agarose 1x TAE gel electrophoresis ...
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bioRxiv - Bioengineering 2021Quote: ... The 319 bp DNA size of PCR products were clarified by 1% agarose gel electrophoresis using 5 μL PCR products and remained DNA were purified by QIAquick PCR Purification Kit (Qiagen, 28104). The purified PCR products were sequenced by Sanger Sequencing approach (GeneWiz ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with selection plasmid pCoBlast (1:10) and the mixture was used for cells transfection by Effectene Transfection Reagent (QIAGEN, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... podocytes were washed three times with warm 1× PBS and the viral DNA was extracted using DNeasy Blood & Tissue kit (Qiagen #69504) [26] ...
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bioRxiv - Plant Biology 2020Quote: ... and thiols and disulphides were extracted in 1 mL of ice-cold 0.1 M HCl using a Tissue-Lyser (Qiagen, Hilden, Germany) and two 3-mm glass beads (30 Hz ...
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bioRxiv - Microbiology 2020Quote: ... frozen in liquid nitrogen for 1 minute and homogenised by shaking at 40 Hz in a Tissue Lyser LT (Qiagen, Germany). The volume of elution buffer AE was adjusted to 200 μL for engorged females ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid sgRNA CRISPR porthos was co-transfected (1 µg of total DNA per well) with Effectene Tranfection Reagent (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted once with 350 μL Phenol:Chloroform:Isoamyl Alcohol (25:24:1) in a phase-lock tube (Qiagen cat. no. 129046) and centrifuged 5 min at 16,000xg followed by extraction with 350 μL chloroform ...
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bioRxiv - Neuroscience 2022Quote: DIV14 neurons treated with 1 µM glutamate for the times indicated in the text then harvested using the Qiagen RNeasy Mini kit (Qiagen, 74104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The qRT-PCR was performed using 20 miRNA primers (Supplementary Table 1) and miScript SYBR Green PCR Kit (Qiagen, Hilden, Germany). RNA U6 was used as the endogenous control ...
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bioRxiv - Cell Biology 2022Quote: ... diluted 2-times and 1 μl was taken for each PCR reaction (20 ul) containing: 0.02 U HotStarTaq® DNA polymerase (Qiagen, Germany), 1 x PCR buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... These specific colonies were amplified by culturing in liquid LB-medium with ampicillin (1:1000) followed by plasmid isolation using QIAprep Spin Miniprep Kit (Qiagen #27104), according to the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... These cells were incubated overnight in MEM-α media free of FBS and supplemented with 1% P/S followed by collection of Supernatant (conditioned media) and total RNA extraction (Cat# 74134, Qiagen, Germany). The resulting samples were stored at −80°C until analyzed further in downstream applications.
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bioRxiv - Molecular Biology 2022Quote: ... In all samples disruption occurred in 1 min intervals to avoid heat generation derived from the TissueRuptor II (catalogue #9002755, Qiagen, MD)29.
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bioRxiv - Developmental Biology 2022Quote: ... Each of the three reaction pellets were allowed to resuspend in TE for 1 hour at room temperature then pooled into one tube and purified across three Qiaquick PCR purification columns (Qiagen, 28104), eluted in the provided Elution Buffer and combined ...