Labshake search
Citations for Qiagen :
2551 - 2600 of 10000+ citations for Mouse 14 3 3 protein sigma SFN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using mouse anti-His antibody from Qiagen (Cat No./ID: 34660 1:500). Anti-mouse HRP was used for colorimetric development (Cat No./ID-A8924 1:1000) ...
-
bioRxiv - Physiology 2022Quote: ... mouse vagal ganglia were removed and placed in sterile cell lysis buffer (Qiagen Puregene, Hilden Germany) with 0.5% SDS and 100ug/ml Proteinase K at 55°C overnight ...
-
bioRxiv - Bioengineering 2019Quote: ... Quantitative real-time-PCR was performed using RT2 Profiler PCR Array for Mouse Mitochondria (330231, Qiagen) with PowerUp SYBR Green (Applied Biosystems ...
-
bioRxiv - Biochemistry 2024Quote: ... PCR arrays were performed using RT2 Profiler™ PCR Array Mouse Cytokines & Chemokines (PAMM-150Z, Qiagen). Calculations were performed by a comparative method (2−ΔΔCt ...
-
bioRxiv - Genetics 2023Quote: ... Mouse Ahrr and Cyp1a1 primers were generated using the PyroMark Assay Design software version 2.0 (Qiagen). Primer sequences can be found in Table S1 ...
-
bioRxiv - Immunology 2023Quote: The RNA extraction process involved lysing mouse colonic IEC or cultured cells using RLT buffer (QIAGEN) and extracting total RNA using the RNeasy Mini Kit (QIAGEN ...
-
bioRxiv - Microbiology 2020Quote: ... to recover parasite material for genomic DNA isolation using a kit (Qiagen DNA Easy Blood and Tissue Kit), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Plasmids and PCR products were purified with QIAprep plasmid Miniprep kit and QiaQuick PCR purification kit respectively (Qiagen). Plasmid Sanger sequencing was performed by Eurofins Genomics ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... using a modified Trizol isolation protocol in combination with a column based commercial kit (QIAGEN RNeasy Mini Kit) based on the manufacturer’s protocol ...
-
bioRxiv - Physiology 2019Quote: DNA of selected LAB was extracted using DNA extraction kit (QIAamp Blood and Tissue Kit, Qiagen, Hilden, Germany). The amplification of 16SrRNA genes were conducted using 27F 5’-AGAGTTTGATCCTGGCTCAG-3’ and 1492R-5’-GGCTACCTTGTTACGACTT-3’primers ...
-
bioRxiv - Microbiology 2021Quote: ... genomic DNA was extracted using the DNAeasy® UltraClean® Microbial kit Isolation Kit (Qiagen Inc., MD, USA) and the near full-length 16S rRNA gene was amplified using primers 8F (5’-AGAGTTTGATCCTGGCTCAG-3’ ...
-
Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: RNA was isolated using Qiagen RNeasy kit and cDNA was generated using the QuantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instruction ...
-
bioRxiv - Genomics 2020Quote: ... and RNA and DNA were extracted (Qiagen RNEasy Mini Kit #74104/Qiagen DNA Blood and Tissue Kit #69504). Clones were again genotyped to verify absence of the mariner insertion (Fig ...
-
bioRxiv - Neuroscience 2021Quote: RNA purification was completed according to the instructions of the manufactured kit (RNeasy® Plus Mini kit; Qiagen). Briefly ...
-
bioRxiv - Microbiology 2022Quote: ... against five other extraction kits: the Qiagen MagAttract PowerSoil Pro DNA Isolation Kit (Cat#: 47109; Qiagen, Carlsbad, CA), the Norgen Stool DNA Isolation Kit (Cat# 65600 ...
-
bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR reaction was carried out using the QuantiNova Kit Probe RT-PCR Kit (Qiagen, catalog #208354, Germany), using primers and probe described in Naveca et al ...
-
bioRxiv - Microbiology 2022Quote: ... The DNA extraction was performed using the PowerSoil DNA extraction kit (DNeasy PowerSoil Pro Kit, QIAGEN, Hilden, Germany), according to the manufacturer’s protocol with an additional 500 μL of 10% (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... Plasmid DNA was extracted using mini-prep plasmid extraction kit and/or Maxi-prep plasmid extraction kit (QIAGEN). The constructs were further confirmed by the Sanger sequencing.
-
bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed utilizing commercial kits and following manufacturer’s protocol (Qiagen’s DNeasy 96 Blood & Tissue Kit, Qiagen). The amount and quality of extracted DNA was evaluated using PicoGreen fluorometer and NanoDrop spectrophotometer (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed utilizing commercial kits and following manufacturer’s protocol (Qiagen’s DNeasy 96 Blood & Tissue Kit, Qiagen). The amount and quality of extracted DNA was evaluated using PicoGreen fluorometer and NanoDrop spectrophotometer (ThermoFisher Scientific) ...
-
bioRxiv - Systems Biology 2023Quote: ... total RNA of the samples was extracted according to manufacturing specifications (RNeasy Protect Bacteria Mini Kit Qiagen kit).
-
bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted using the genomic DNA purification kit (Dneasy Blood and Tissue kit, Cat. 69504, Qiagen), the specific fragment containing rG4-1 sequence was amplified using Phire Green Hot Start II PCR Master Mix (F126S ...
-
bioRxiv - Microbiology 2023Quote: Total DNA and mRNA were isolated using QIAamp DNA mini kit and RNeasy mini kit (Qiagen, Germantown, MD), per the manufacturer’s recommendation ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The selected BACs were recovered and sequenced on an Illumina MiSeq using reagent kit Index Nextera XT kit V2-500 cycles and assembled with the CLC Genomics Workbench 20.0 v7.5 (QIAGEN). P ...
-
bioRxiv - Molecular Biology 2023Quote: ... All plasmids were purified with a Midiprep kit (ZymoPURE, #D413) or a QIAprep spin miniprep kit (QIAGEN, #27104) and verified by DNA sequencing prior to use ...
-
bioRxiv - Genomics 2023Quote: Total RNA from tissues was extracted using a commercial kit (RNeasy Plus Mini Kit; Qiagen, Toronto, ON, Canada). Quality control checks ...
-
bioRxiv - Genomics 2023Quote: ... We used the MagAttract® HMW DNA kit and the DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany) for DNA isolation ...
-
bioRxiv - Cancer Biology 2024Quote: ... DNA was isolated using either QIAGEN Plasmid Plus Midi Kit or 28 QiaPrep Spin Miniprep Kit (Qiagen, 27104).
-
bioRxiv - Genomics 2023Quote: DNA from both types of samples was extracted using a commercially available kit (DNeasy Blood & Tissue Kit, QIAGEN) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted using the QIAamp DNA Blood Midi Kit or QIAamp DNeasy Blood & Tissue Kit (QIAgen). Genome sequencing libraries were made using Illumina genome DNA prep reagents (Illumina ...
-
bioRxiv - Synthetic Biology 2024Quote: ... NEB) and purified using a Qiagen extraction kit as per the manufacturer’s instructions (QIAquick Gel Extraction Kit, Qiagen). A complete list of plasmids included in this study can be found in Related Manuscript File 2 ...
-
bioRxiv - Evolutionary Biology 2021Quote: Protein sequence alignments and tree production were made using the CLC main work bench (ver. 5.5.1, Qiagen) software package ...
-
bioRxiv - Plant Biology 2019Quote: Sitting-drop vapour-diffusion experiments identified suitable crystallisation conditions for native protein from the PACT screen (QIAGEN), with optimisation identifying the best conditions for crystal formation as 6 mg/mL protein combined 1:1 with 8% PEG 1500 ...
-
bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and His-tagged protein was purified from cell lysate using a Ni-NTA spin column (Qiagen, 31014). The resulting product was run on a 12% Bis-Tris PAGE gel (Thermofisher ...
-
bioRxiv - Molecular Biology 2020Quote: Nme2Cas9 protein was expressed in Rosetta (DE3) cells and purified using a Ni2+-NTA agarose column (QIAGEN) as previously described24 ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.2 mM TCEP) the expressed protein was extracted from the supernatant using NiNTA resin (QIAGEN, Germantown, MD). The resulting eluate from the NiNTA resin using lysis buffer supplemented with 250 mM Imidazole pH 7.4 ...
-
bioRxiv - Microbiology 2022Quote: ... 6xHis tagged protein were purified by immobilized metal ion chromatography using nickel-NTA super flow resin (Qiagen) as previously described with slight modifications46 ...
-
bioRxiv - Plant Biology 2021Quote: Alignments of histone DNA and protein sequences was performed using CLC Main Workbench software version 8.1 (QIAGEN). Predictions of intrinsic disorder were undertaken by PONDR (58) ...
-
bioRxiv - Immunology 2022Quote: ... https://www.beiresources.org/).Protein was purified using gravity flow purification with Ni-nitrilotriacetic acid (NTA) agarose (Qiagen, Germany) and concentrated and buffer exchanged in Amicon centrifugal units (EMD Millipore ...
-
bioRxiv - Immunology 2019Quote: His-tag purified recombinant proteins were expressed in E.coli and purified by Ni+-NTA-agarose column (Qiagen). Then ...
-
bioRxiv - Biochemistry 2019Quote: ... Expressed protein was purified by elution from a nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Germantown, MD) column with 0.5 M imidazole and buffer exchanged into TEV buffer (25 mM NaHPO4 ...
-
bioRxiv - Genomics 2021Quote: ... RNA-protein complexes were then incubated with Ni-NTA sepharose (Qiagen, 100 μl of slurry per sample) overnight at 4°C and extensively washed ...
-
bioRxiv - Developmental Biology 2020Quote: ... The significantly changed proteins were used as inputs for Ingenuity Pathway Analysis (IPA, Version 03-13, Qiagen). The IPA predicted the altered pathways ...
-
bioRxiv - Biochemistry 2021Quote: ... All the protein samples were expressed in BL21(DE3) and purified using Ni-NTA Sepharose column (QIAGEN) and Superdex 200 16/600 column (GE Healthcare).
-
bioRxiv - Biochemistry 2021Quote: ... The polyhistidine-tagged recombinant proteins were then purified from bacterial lysates with Ni2+-NTA agarose beads (QIAGEN) and washed by three different washing buffers (Washing buffer 1 contained 20 mM Tris-HCl ...
-
Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... 0.2 µg of AFP1-His proteins was incubated with 20 µl Ni-NTA agarose beads (Qiagen, Germany) in buffer (25 mM Tris-Cl ...
-
bioRxiv - Immunology 2020Quote: ... HIS-tagged DR protein monomers were produced by Hi5 insect cells and purified using Ni-NTA (Qiagen) and size exclusion columns (AKTA ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen, Germantown, Maryland). Detection of proteins via Western blotting was performed by fluorescence detection using IR-Dye®-labeled fluorescent secondary antibodies and imaged using the Odyssey CLx Imager (LICOR Biosciences ...
-
bioRxiv - Plant Biology 2023Quote: ... The His-ZmTPL2N-His recombinant protein was purified using Pierce Ni-NTA resin (QIAGEN, Cat. No. 30210) according to the product protocol ...