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Citations for Qiagen :
2501 - 2550 of 10000+ citations for Mouse Starch Binding Domain Containing Protein 1 STBD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... from the top A horizon were immediately collected for DNA extractions in 15 mL tubes containing 5 mL Lifeguard Soil Preservation Solution (QIAGEN) using chilled soil-processing trays ...
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bioRxiv - Genetics 2020Quote: ... Bacterial cells lysis was then achieved using Pathogen Lysis Tubes (containing beads) and a lysis buffer in the TissueLyser LT instrument (QIAGEN). Lysates were transferred to QIAamp UCP Mini Columns and bound DNA was eluted in 50 μL of elution buffer ...
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bioRxiv - Genomics 2019Quote: ... For the bacteria-specific 341F/805R47 primer pairs a different reaction mixture was used containing 10x Standard Taq Reaction buffer (Qiagen), 2 mM MgCl2 ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatant was applied twice to a column containing a gel bed of 2 ml nickel-nitriloacetic acid agarose (Qiagen, www.quiagen.com) equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads containing long DNA fragments were separated on a magnet and the supernatant containing only small DNA fragments below roughly 800 bp were cleaned up using MinElute PCR purification columns (Qiagen). All libraries were amplified for 12 cycles in total ...
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bioRxiv - Biochemistry 2019Quote: ... Cell lysate was separated by centrifugation at 28000 x g for 45 min and 4°C and the supernatant was loaded onto a column containing 2 mL of Ni-NTA agarose (Qiagen) equilibrated with buffer A for 1 h for purification by nickel affinity chromatography ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing His-eIF4A1 or His-eIF4A2 was incubated with a 1.5 ml bed volume of Ni-NTA Superflow (Qiagen, 30430) or a 0.75 ml bed volume of Ni-NTA agarose (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... an amplicon containing a SNP at the Cast allele was amplified by PCR from cDNA using Hot Start Taq (Qiagen) for 38 cycles ...
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bioRxiv - Genomics 2021Quote: ... were sorted into individual wells of a 96 well plate containing lysis buffer 2.5μL RLT Plus Lysis Buffer (QIAGEN, catalog # 1053393) with 1U/μL SUPERase-In (ThermoFisher ...
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bioRxiv - Genomics 2020Quote: ... 25 nuclei were sorted into each well of 96-well plates (8-10 plates per experiment) containing 12 µl of nuclear lysis buffer (11 µl of EB buffer (Qiagen) supplemented with 0.5 µl of 100X BSA and 0.5 µl of 1% SDS) ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were removed and the remaining cell pellet (500 μl) was transferred into a 15 ml tube containing 2.5 ml RNAprotect® Cell Reagent (Qiagen). The cells were resuspended by vortexing ...
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bioRxiv - Microbiology 2020Quote: ... The DNA template for standard curves was prepared by PCR performed in 50 μl reactions containing 25 μl Taq PCR Mastermix (Qiagen), 19 μl PCR-certified water ...
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bioRxiv - Microbiology 2021Quote: ... Mosquito bodies and legs and wings were put into a 2 ml round-bottom tubes containing 250 ml of PBS and a steel ball (Qiagen). Samples were homogenized using a TissueLyser (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... in 96 flat bottom plates in the presence or absence of PMA (200ng/ml) and ionomycin (300ng/ml) were lysed in 100Sµl lysis buffer containing TCL buffer (Qiagen, 1031576) containing 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: The resulting sequencing data was analyzed by mapping reads to a database containing 60 bases of sequence flanking each secondary gene using CLC Genomics Workbench (Qiagen). Total read counts at each locus were normalized to the secondary gene with the greatest number of reads in each sample ...
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bioRxiv - Immunology 2021Quote: Single cells were index-sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, QIAGEN 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Developmental Biology 2022Quote: ... Stable cell lines were obtained by transfection with pMT puro vectors containing either Zasp52-PF or Zasp52-PF-ABM using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... an amplicon containing a SNP at the Cast allele was amplified by PCR from cDNA using Hot Start Taq (Qiagen) for 38 cycles ...
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bioRxiv - Microbiology 2022Quote: ... Left lungs were harvested in 2 ml Eppendorf (Stevenage, UK) Safe-Lock micro test tubes containing 500 µl DMEM and a stainless-steel carbide bead (Qiagen), and snap frozen on dry ice until virus titration ...
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bioRxiv - Microbiology 2024Quote: ... 10 Arabidopsis seedlings from the same MS plate were sampled together in 2 mL microtubes containing two 3 mm-diameter tungsten carbide beads (Qiagen), and flash frozen in liquid nitrogen ...
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bioRxiv - Genetics 2024Quote: ... they were transferred to Eppendorf tubes containing either 180 µL buffer ATL and 20 µL Proteinase K (Qiagen, Hilden, Germany), or 350 µL of in-house ZB5d medium/antibiotics mixture as described in Ries et al ...
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bioRxiv - Plant Biology 2024Quote: ... The fresh leaf discs were transferred to a 2 mL tube containing a 5 mm stainless steel bead and 500 µL buffer RLT (QIAGEN). The leaf discs were disrupted using a TissueLyser LT (QIAGEN ...
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bioRxiv - Genetics 2023Quote: ... RNA was extracted from 1/5th of the total volume of input WCEs and from 300 µl of each set of pooled fractions containing 80S or different polysomal species using QIAzol lysis Reagent (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Twenty-four hours later 100,000 cells were transferred to a tube containing 100 µl of RLT buffer (Qiagen, Hilden, Germany). Cells were stored at –80 °C until further processing ...
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bioRxiv - Biophysics 2023Quote: ... beads were discarded and the supernatant containing reconstituted into ND A2AAR was incubated for 4 h with 250 µL of Ni-NTA resin (Qiagen) for separating from empty ND ...
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bioRxiv - Microbiology 2023Quote: ... The cleared lysate was applied to an affinity chromatog-raphy column containing Ni-nitrilotriacetic acid (NTA) su-perflow resin (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Genomics 2022Quote: ... We discarded the flowthrough of the PVDF membrane and transferred the membrane immediately after filtration to a 0.7 mm Garnet particle containing tube (13123-50, MoBio – now QIAGEN PowerBead) using sterile tweezers ...
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bioRxiv - Genomics 2023Quote: ... The BD FACS Melody sorter device was used for sorting single cells into 96-well plates (FrameStar®, 4TI-0960/C) containing 2.5 μl RLT plus buffer (Qiagen). Plates were then spun down at 1,000g for 1 minute at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... calf muscle and pancreas were collected in 500 μl of PBS containing one (heart and pancreas) or two (calf muscle) 5 mm stainless steel beads (QIAGEN), homogenized with TissueLyser II (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... containing RNA derived from the entire cortical sample was collected and lysed with 350 µl of buffer RLT from Qiagen RNeasy Kit supplemented with 10μl/ml bME ...
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bioRxiv - Immunology 2023Quote: ... The first round of PCR was carried out using the ImmunoSEQ proprietary PCR primer mix (32 μL per sample containing 25 uL of QIAGEN 2× Multiplex PCR Master Mix ...
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bioRxiv - Neuroscience 2023Quote: ... the upper aqueous phase containing RNA was mixed with equal amounts of 100% ethanol and was then loaded onto an RNA spin column (Qiagen) and centrifuged for 30 seconds at 8000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Triplicate PCRs were carried out in 15 µl reactions containing 1x UCP Multiplex PCR Master Mix (Qiagen, Venlo, The Netherlands), 0.3 μmol l−1 each of the forward and reverse primers ...
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bioRxiv - Neuroscience 2023Quote: ... GFP+ and GFP- nuclei were sorted using a BD AriaFACS III (University of Washington Pathology Flow Cytometry Core) into a PCR tube strip containing 3 µL of REPLI-g Advanced Single Cell Storage buffer (Qiagen). Whole genome amplification (WGA ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were collected continuously into 50 mL conicals containing 20 mL RNAlater (saturated ammonium sulfate, 20mM EDTA, pH 5.2; QIAGEN #76106) and a stirring magnetic stir disc (V&P Scientific #772DP-N42-5-2) ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were incubated with a PCR mix containing (100 µM dNTP, 1X thermopol Taq reaction buffer, 1X of Q solution (Qiagen) and 1 µM of each primer) ...
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bioRxiv - Microbiology 2023Quote: ... in vitro in L-lactate containing minimal media and total RNA was isolated by the TRIzol method and RNeasy minikit (Qiagen). After digestion with TURBO Dnase treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was thoroughly aspirated and replaced with 20 µl of 1x PBS containing 5 mg/ml of RNase A (Qiagen) and 12 mM of purified H6-mNeonGreen-NLS protein as a loading control ...
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bioRxiv - Physiology 2024Quote: ... with 250uL LCMS-grade water and 215uL LCMS-grade MeOH containing 10uL SPLASH lipidomix standard (Avanti #330707) and homogenized in a TissueLyzer (Qiagen) at 4C ...
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bioRxiv - Genomics 2020Quote: ... PCR products were gel-purified with a DNA gel extraction kit (QIAquick Gel Extraction Kit, Qiagen) and used for library preparation with TruSeq/ChIP-Seq reagents (Illumina) ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA isolation was then carried out using a RNA extraction Kit (RNeasy Mini Kit, Qiagen). scRNA-seq was performed using the Smart-seq2 protocol68 ...
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bioRxiv - Molecular Biology 2020Quote: ... total DNA was purified from supernatant using commercially available kit (DNeasy 96 Blood & Tissue kit, Qiagen). The HBV DNA was amplified in a real-time PCR assay using LightCycler 480 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA was purified with the PeqGOLD Total RNA Kit (PeqLab) or RNeasy Mini Kit (Qiagen) following the manufacturer’s instruction and an additional DNAse I digestion was performed on the column provided in the kit ...
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bioRxiv - Microbiology 2019Quote: ... using the QIAamp Viral RNA Mini Kit or QIAamp Circulating Nucleic Acid kit (Qiagen, Hilden, Germany). Total nucleic acid was eluted in two centrifugation steps with 40 µl of Buffer AVE each ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were purified using the QIAprep Spin Miniprep Kit or the HiSpeed Plasmid Maxi Kit (QIAGEN). Nucleotide sequences of all cloned constructs were confirmed by DNA sequencing (Eurofins Genomics) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... RNA eluate was purified with the RNeasy RNA clean up kit RNeasy Mini Kit (Qiagen, NL) as per protocol ...
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bioRxiv - Genetics 2020Quote: ... dhfr products were excised from the gel using commercial kits (QIAquick Gel Extraction Kit, Qiagen, Germany). 20 μl of eluted DNA was then purified using AMPure XP Magnetic Beads (1X ...
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bioRxiv - Biochemistry 2020Quote: Total RNA was prepared from frozen plant material using plant RNA purification kit (QIAGEN RNeasy Kit). Following electrophoresis (1.5%(w/v ...
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bioRxiv - Microbiology 2021Quote: ... The QIAquick Gel Extraction Kit and the QIAquick PCR Purification Kit (QIAGEN Inc, Valencia, CA, USA) were used for PCR- product clean-up ...
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bioRxiv - Cell Biology 2020Quote: ... and was retro-transcribed using the Qiagen reverse transcription kit (miScript II RT Kit, #218161, Qiagen) and pre-amplified using miScript PreAMP PCR Kit (# 331451 ...