Labshake search
Citations for Qiagen :
2451 - 2500 of 4479 citations for 6 METHYL 2 OXO 4 THIOPHEN 2 YL 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Total RNA from cells in a single well of a 6-well plate was isolated using the RNeasy Plus Mini Kit (Qiagen, 74134) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2019Quote: RNA from myotubes grown in 6-well plates in conditions described above was isolated using the RNeasy Micro Kit from Qiagen (#74004). Quality of RNA was assessed by using a Bioanalyzer 2100 and samples were submitted for library preparation and deep sequencing to the Molecular biology core facility at the Dana Farber Cancer Institute ...
-
bioRxiv - Bioengineering 2019Quote: ... The inclusion body pellets were resuspended in 20 mM Tris-HCl buffer with 500 mM sodium chloride and 6 M urea (pH=8.0) and incubated with Ni-NTA resin (Qiagen, Valencia, CA) for 2 hours at 4°C ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was isolated from iPSC-SNs seeded in 6-well plates using a RNeasy Mini Kit (Qiagen, Redwood City, CA) and reverse transcribed into cDNA using a SuperScript VILO cDNA Synthesis kit (Life Technologies ...
-
bioRxiv - Cell Biology 2021Quote: Cells were grown to 80% confluence in a 6-well plate before RNA was extracted using standard RNA extraction protocol (RNAeasy Mini Kit, Qiagen, #74104). cDNA synthesis was performed by mixing 1μg of RNA with qScript® cDNA Synthesis Kit (Quantabio ...
-
bioRxiv - Physiology 2022Quote: ... liver and eWAT samples (n=6 per group) were extracted using RNABle lysis reagent (Eurobio) and the RNeasy® Mini Kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: Total RNA from hippocampus samples (n=6 per group) was extracted using RNABle lysis reagent (Eurobio) and the RNeasy® Mini Kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: DNA was extracted from GWSS and control samples (nGWSS = 79, ncontrol = 6) using a DNeasy PowerSoil DNA Isolation kit (Qiagen, Germany) with minor changes to the manufacturer’s protocol as follows ...
-
bioRxiv - Cancer Biology 2023Quote: ... BON1/TGL/pQ and BON1/TGL/HA-Bcl-xL) grown on 6-cm dishes were used to isolate mRNA using RNeasy Plus Mini kit (Qiagen, 74136) containing gDNA Eliminator spin columns ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA was isolated from cell pellets in batches of 6 using an RNeasy Plus Mini Kit (Cat # 74134, Qiagen, Hilden, Germany). Acceptable RNA concentration and quality were verified with Nanodrop and Bioanalyzer measurements ...
-
bioRxiv - Bioengineering 2023Quote: ... DNA was extracted in pools of 6-8 adult mosquitoes mosquitoes using Blood & Cell Culture DNA Midi Kit (Qiagen, Cat# 13343) following the manufacturer’s protocol ...
-
Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... RNA and protein were extracted 6 days post-transduction using the AllPrep RNA/Protein kit (Cat: 80204, Qiagen, Germantown, MD USA). RNA was converted to cDNA using SuperScript IV cDNA Synthesis Kit (Cat ...
-
bioRxiv - Neuroscience 2021Quote: ... Hsa-miR-124-3p Locked Nucleic Acid (LNA) power inhibitor and has-miR-124- 3p miRCURY LNA miRNA mimic (Qiagen, Germantown, MD) were resuspended with RNase-free water to 100 μM ...
-
bioRxiv - Systems Biology 2021Quote: Approximately 10 mL of amniotic fluid from each animal was used for cell-free RNA extraction with the QIAamp Circulating Nucleic Acid Kit (QIAGEN, Valencia, CA). RNA from the lung and hippocampus was extracted with the RNeasy Universal Mini Kit (QIAGEN ...
-
Cell-free DNA as a biomarker for prostate cancer: elevated concentration and decreased fragment sizebioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from 7 to 55 mL of plasma using the Qiagen QIAamp Circulating Nucleic Acid Kit (Qiagen, Redwood City, CA), and double eluted with 40 μL of Qiagen Elution Buffer ...
-
bioRxiv - Cell Biology 2020Quote: RNA FISH was performed using a FITC-labeled DNA oligo probe complementary to alpha satellite (Integrated DNA Technologies, Newark, NJ) or a biotinylated locked nucleic acid (LNA) oligo probe complementary to HSATII (QIAGEN, Hilden, Germany) (see Key Resources Table) ...
-
bioRxiv - Molecular Biology 2022Quote: ... hirae V-ATPase was expressed recombinantly in Escherichia coli and purified by affinity purification with a Ni+-NTA (nitrilotriacetic acid) column (Ni+-NTA Superflow; Qiagen, Hilden, Germany). The column was preconditioned with buffer consisting of 50 mM potassium phosphate ...
-
bioRxiv - Microbiology 2023Quote: The amino acid sequences of coronavirus spike orthologues were subjected to multiple alignment using CLC Workbench 7 (CLC Bio, Qiagen, Aarhus, Denmark). Protein sequence NCBI reference sequences for the indicated viruses are as follows ...
-
bioRxiv - Biophysics 2024Quote: ... The clarified lysate was then applied to a 10 mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...
-
bioRxiv - Microbiology 2024Quote: Total nucleic acid (DNA and RNA) was extracted from BDB by PowerSoil DNA Isolation Kit or PowerSoil Total RNA Isolation Kit (Qiagen, Hilden, Germany). qPCR was carried out for viral and protozoan pathogens including enterovirus ...
-
bioRxiv - Microbiology 2020Quote: ... plasmids were prepared from 5 ml overnight culture using Miniprep kits (Qiagen) and the plasmids were sequenced using M13F-M13R primers (Table S4).
-
bioRxiv - Cell Biology 2020Quote: ... RNA was extracted from 5 million cells with RNeasy mini Kit (Qiagen) following the manufacturer’s instructions and with DNase treatment on column ...
-
bioRxiv - Immunology 2020Quote: ... The cell pellet was resuspended in 5 ml RBC lysis buffer (Qiagen), incubated at room temperature for 5 minutes and then washed twice with 45 ml RPMI ...
-
bioRxiv - Molecular Biology 2020Quote: Cells were collected in 5 µl cold RLT Plus buffer (Qiagen, 1053393) containing 1U/µl RNAse inhibitor (Ambion ...
-
bioRxiv - Immunology 2019Quote: ... homogenized with a single 5 mm steel bead in a TissueLyzer (Qiagen) at 30 Hz for 3 min ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... 5 μL Qiagen master mix (Qiagen Type-It Microsatellite Kit, Qiagen Inc.), 0.2 μL of each reverse primer ...
-
bioRxiv - Genomics 2020Quote: ... 5 pM of each primer and 2.0 U HotStar Taq polymerase (Qiagen) in a 25 µl volume or 1X Phusion U Hotstart (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... Lysates were loaded onto a 5 mL Ni-NTA Superflow Cartridges (Qiagen) equilibrated with Buffer B (25 mM HEPES ...
-
bioRxiv - Biochemistry 2022Quote: ... The lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed with lysis buffer supplemented with 25 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed in two steps with lysis buffer supplemented with 25 mM and 100 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was incubated with 5 ml of Ni-NTA resin (Qiagen) for 2h ...
-
bioRxiv - Microbiology 2021Quote: ... and homogenized using 5 mm stainless-steel beads and a TissueLyser (Qiagen). Virus titers in serum and organ homogenates were determined by plaque assay using L929 cells [86] ...
-
bioRxiv - Cancer Biology 2020Quote: ... 5 µg RNA was DNase treated using RNase-Free DNase Set (Qiagen). 1 µg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs) ...
-
bioRxiv - Microbiology 2020Quote: ... 5 μl RNA was tested with the Rotor-GeneTM probe kit (Qiagen) according to instructions of the manufacturer ...
-
bioRxiv - Molecular Biology 2021Quote: ... The cleavage reaction was run over a 5 mL Streptactin column (Qiagen) to remove any uncleaved HELB and free StrepII peptide and the cleaved HELB-containing flow-through collected ...
-
bioRxiv - Immunology 2020Quote: Total RNA from 5×106 PBMCs was isolated using RNeasy kits (Qiagen). First-strand cDNA was generated from total RNA using SuperScript RT IV (Invitrogen ...
-
bioRxiv - Genetics 2019Quote: ... 0.1 μl of HotStarTaq DNA polymerase (5 U/μl; Qiagen, Düsseldorf, Germany) and 11.9 μl of nuclease-free water ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 μg RNA was DNase treated using RNase-Free DNase Set (Qiagen) and 1 μg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tissue homogenization was performed with stainless steel beads of 5 mm (Qiagen) and TissueLyser LT (Qiagen) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... with two 5 mm stainless beads using a TissueLyser (Qiagen, CA, USA) at 50 Hz for 4 min ...
-
bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
-
bioRxiv - Biochemistry 2021Quote: ... The lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed with lysis buffer supplemented with 25 mM imidazole ...
-
bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed in two steps with lysis buffer supplemented with 25 and 100 mM imidazole ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cleared lysate was loaded on a 5 mL Ni-NTA column (Qiagen) equilibrated with buffer C ...
-
bioRxiv - Molecular Biology 2021Quote: ... Worms were homogenised with a 5 mm diameter stainless steel bead (Qiagen) for 4 min at 50 Hz in 100 µl of 0.15 M McIlvaine buffer pH 4.6 [64] with EDTA-free protease inhibitors (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... RNA was isolated from 5×106 cells using the RNeasy minikit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... the reaction tubes were added 5 μL of PureGene Proteinase K (Qiagen) and incubated for additional 30 minutes at 50°C ...
-
bioRxiv - Microbiology 2022Quote: ... then 5 µl of RNAse A (10 mg/ml; Qiagen, Hilden, Germany) was added and the sample was incubated for 30 min at 37 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... a proteinase K reaction solution containing 5 μL of PKD buffer (QIAGEN) and 0.31 μL of ProK (QIAGEN ...