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Citations for Qiagen :
201 - 250 of 1193 citations for Recombinant Human CSF2 Protein non tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The His-tagged nanobodies were purified by using Ni-NTA purification column (Qiagen) followed by desalting step using disposable PD-10 desalting columns (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... His-tagged SLFN11 (residues 349-901) was purified by Ni-NTA beads (Qiagen). All purified GST- or His-tagged proteins were dialyzed against a buffer containing 50 mM Tris-HCl and 150 nM NaCl and validated by Coomassie blue-stained SDSLJPAGE.
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757 ...
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bioRxiv - Developmental Biology 2021Quote: ... The non-targeting control siRNA (NTC) used was from Qiagen GeneGlobe (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... and a non-targeting siRNA (All-Star negative control, QIAGEN).
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bioRxiv - Cell Biology 2020Quote: ... EpiTect methylated and non-methylated bisulfite-treated control DNA (Qiagen) was used to evaluate the quantitative recapture of methylation ratios of the amplicons ...
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bioRxiv - Molecular Biology 2021Quote: ... and a Negative Control non-targeting siRNA were from Qiagen and transfected with lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Non-targeting siRNA was purchased from Qiagen (Catalog No. – 1027281) and used as a negative reference control ...
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bioRxiv - Microbiology 2022Quote: ... Gene expression of 84 heat shock genes in mock-infected or 229E-infected cells was analyzed using Human Heat Shock Proteins & Chaperones RT2 Profiler PCR array (Qiagen). Real-time PCR analyses were performed with specific primers ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transiently transfected with HA-tagged RILP or ORP1L constructs using Effectine (Qiagen), harvested at 24 hours post-transfection and fixed in 3.7% formaldehyde in PBS for 15-20 min ...
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bioRxiv - Microbiology 2020Quote: ... His6-tagged Lem27 or its truncation mutants was purified using Ni2+-NTA columns (Qiagen). After washing with a buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
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bioRxiv - Genetics 2019Quote: ... Non-targeting control siRNA was the Allstars Negative Control siRNA (Qiagen).
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Non-targeting control siRNA was the Allstars Negative Control siRNA (Qiagen). siRNA targeting GM130 and scrambled negative control siRNA were purchased from OriGene ...
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bioRxiv - Molecular Biology 2023Quote: ... A non targeting sequence siRNA (AllStars Negative Control QIAGEN Cat # 10272281) was used as negative control ...
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bioRxiv - Genomics 2022Quote: ... Non-methylated (0%) and fully methylated (100%) standards (Qiagen, Hilden, Germany) were used as controls to examine the consistency of methylation levels across all batches ...
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bioRxiv - Biophysics 2021Quote: ... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
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bioRxiv - Cancer Biology 2020Quote: ... Pyrosequencing of biotinylated PCR products was performed using PyroMark Q48 Advanced CpG reagents (Qiagen) on a Pyromark Q48 Autoprep apparatus (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tagged AC was secreted into the medium and purified by nickel affinity chromatography (Qiagen) with elution in 100 mM citrate pH 4.0.
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bioRxiv - Neuroscience 2020Quote: ... Recombinant plasmids were purified with EndoFree Plasmid Maxi Kit (12362, QIAGEN). Oligonucleotide sequences and further details are provided in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant 6xHis-Uhrf1 was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 40,920 cm-1 M-1) ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant 6xHis-Swi6 was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 45,330 cm-1 M-1) ...
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bioRxiv - Immunology 2020Quote: ... Recombinant HCMVs were reconstituted from HCMV BAC DNA by Superfect (Qiagen) transfection into permissive MRC-5 cells.
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bioRxiv - Genetics 2022Quote: ... human SETD2 siRNA (Qiagen # 1027416 ...
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bioRxiv - Genetics 2022Quote: ... human CRY2 siRNA (Qiagen # 1027416 ...
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bioRxiv - Genetics 2022Quote: ... human CPSF6 siRNA (Qiagen # 1027416 ...
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bioRxiv - Cell Biology 2020Quote: ... The AllStars Negative siRNA non-targeting sequence was purchased from Qiagen (#SI03650318).
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bioRxiv - Developmental Biology 2020Quote: ... Pax6 and non-specific control siRNA were used (predesigned FlexiTube siRNAs, Qiagen). For siRNA transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Non-targeting siRNA controls were siC (Qiagen All-Stars negative control,1027281) or siNT1 (D-001810-10-05 ...
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bioRxiv - Cell Biology 2021Quote: ... Non-targeting siRNA (All-star Negative Control siRNA) was purchased from QIAGEN.
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bioRxiv - Immunology 2022Quote: Tregs and non-Tregs were lysed in RLT buffer (Qiagen, Hilden, Germany) and frozen at −80°C ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was extracted from non-clonal samples using the DNeasy Kit (Qiagen). DNA for clone screens was extracted by QuickExtract (Lucigen ...
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bioRxiv - Immunology 2020Quote: ... or non-targeting AllStars negative control siRNA (1027280) were purchased from Qiagen. All siRNA transfections were performed using the Lipofectamine RNAiMax reagent (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... Non-tissue samples were inactivated by placing samples into AVL buffer (Qiagen) and adding 100% ethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... and compared to a non-targeting siRNA control (Qiagen Allstars Neg Ctrl). 786-O cells were split one day prior to transfection and plated at 50-60% confluency ...
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bioRxiv - Biochemistry 2023Quote: C2C12 cells were transfected with 200 nM non-target siRNA (siScr, Qiagen) or siNr2f6 (Sigma ...
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bioRxiv - Genomics 2023Quote: ... Button valves were opened and biotinylated anti-pentaHis antibody (Qiagen, 1:4 dilution in HEPES) was flowed for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged BASP1 derivatives were prepared using Ni-NTA beads following the manufacturers instructions (Qiagen). Purified proteins were dialysed into Buffer D and stored at −80°C.
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bioRxiv - Immunology 2022Quote: ... and His-tagged Fabs were isolated from cell supernatants using Ni-NTA columns (Qiagen, Hilden, Germany). IgGs ...
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bioRxiv - Cell Biology 2020Quote: ... the resolubilized histidine-tagged histones were purified with Ni affinity chromatography using Ni-NTA beads (Qiagen). For untagged H2B ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the polyhistidine-tagged toxin was loaded onto a Ni-NTA agarose column (Qiagen). The column was then washed with a 20–120 mM gradient of imidazole to remove additional contaminating proteins ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged DmMIC10b was detected using an anti-His antibody (Qiagen N.V., Venlo, The Netherlands, 34660).
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bioRxiv - Bioengineering 2023Quote: ... Recombinant plasmids were prepared by using the QIAprep Spin Miniprep Kit (QIAGEN) and confirmed by sequencing analysis ...
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bioRxiv - Microbiology 2021Quote: ... The AllStars negative control siRNA was used as a non-specific control (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... A non-silencing RNAi oligonucleotide (Allstars negative control siRNA) was purchased from Qiagen. Cells were seeded on coverslips at densities of 1.2–4 × 104 cells and transfected with siRNAs at final concentrations of 10–17 nM using RNAiMax (Life Technologies Corporation ...
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bioRxiv - Cell Biology 2021Quote: ... A non-silencing RNAi oligonucleotide (Allstars negative control siRNA) was purchased from Qiagen. Cells were seeded on coverslips at densities of 1.2–4 × 104 cells and transfected with siRNAs at final concentrations of 10–17 nM using RNAiMax (Life Technologies Corporation ...
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bioRxiv - Zoology 2021Quote: ... or a non-destructive approach using the DNeasy Blood and Tissue Kit (Qiagen). For the non-destructive method ...
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bioRxiv - Molecular Biology 2022Quote: ... was used to transfect HNRNPK smartpool siRNA (Dharmacon) or non-targeting siRNA (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: We included the same non-targeting negative control (AllStars Negative Control siRNA, Qiagen) and transfection efficiency control (AllStars Hs Cell Death Control siRNA ...
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bioRxiv - Genomics 2020Quote: ... together with 0.2 uM of each biotinylated primer and 0.25 units of HotStarTaq PlusDNA Polymerase (Qiagen). Refer to Supplementary Table 10 for primer sequences ...