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Citations for Qiagen :
201 - 250 of 10000+ citations for Rat Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... chromatography columns containing Ni-NTA agarose (Qiagen) equilibrated in binding buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Genomics 2023Quote: ... containing 0.125µl protease (Qiagen, cat. no. 19155), 0.075μl 10% triton X-100 ...
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bioRxiv - Biochemistry 2023Quote: ... chromatography columns containing Ni-NTA agarose (Qiagen) were equilibrated in binding buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Genetics 2024Quote: ... containing 0.2 mg/mL Proteinase K (Qiagen) for 10 minutes at 56°C ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Microbiology 2021Quote: ... Whole ear tissues were homogenised in sterile 1.5 ml flat-bottom tubes containing 400 µl of PowerBead Solution (Part of DNeasy UltraClean Microbial Kit; Qiagen, Cat #12224-250, Hilden, Germany) with an OMNI Tissue Homogeniser with plastic hard-tissue probes (OMNI International) ...
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bioRxiv - Zoology 2023Quote: ... Then 25-50 mg of rehydrated tissue was cut into small pieces with a sterile disposable scalpel blade and transferred into individual 2 ml Eppendorf tubes containing 180 µL of ATL and 20 µL of proteinase K provided by the IndiSpin® QIAcube® HT Pathogen Kit (Qiagen, Courtaboeuf, France). Tubes were incubated between 6 and 12 h at 56°C until full proteolysis was achieved ...
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bioRxiv - Physiology 2023Quote: RNA was extracted from MLEC and lung tissue by lysis in RLT buffer containing 1% β-mercaptoethanol for RNA isolation and subsequently purified using the RNeasy Mini Kit (Qiagen, 74106, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... the song control nucleus HVC and the visual area of the entopallium were dissected from the abovementioned 40-μm sections under a stereomicroscope (typically 24-32 slices for the HVC and 16-20 slices for the entopallium) and transferred into an Eppendorf tube containing 340 μl of RLT buffer mixture (containing DTT, Qiagen). This dissection procedure using rather thin sections reduces the contamination of HVC tissue with surrounding tissue ...
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bioRxiv - Immunology 2020Quote: ... ConM-SOSIP.v7 carrying a C-terminal His-tag (diluted to 6.5nM in TBS) was immobilized onto 96-well Ni-NTA ELISA plates (Qiagen) by incubation for 2 hours at room temperature after which the plates were washed 3 times with TBS ...
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bioRxiv - Cell Biology 2021Quote: ... containing 500 µL of Ni-NTA agarose (Qiagen) preequilibrated with Buffer L at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... containing 10uL of lysis buffer (TCL buffer, Qiagen 1031576 supplemented with 1% beta-mercaptoethanol) ...
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bioRxiv - Microbiology 2022Quote: ... coli strains containing expression plasmid pQE-9 (Qiagen), pQE9-ftsA ...
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bioRxiv - Microbiology 2022Quote: ... containing 5 mm diameter stainless steel beads (Qiagen) and 150 μL DMEM supplemented with 2% FBS and homogenized using a TissueLyser II (2 cycles at 30 Hz ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... containing protease inhibitor cocktail using PowerLyser 24 (Qiagen) and mixed gently at 4 °C for one hour ...
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bioRxiv - Neuroscience 2022Quote: ... and aged (n=10) RVLM-containing tissue punches was reverse transcribed to cDNA using an RT2 preamp cDNA synthesis kit (Qiagen, Inc., First strand cDNA synthesis) following manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: Pathway-specific primer mixes (Rat Antibacterial Response, PBR-148Z, and Human Antibacterial Response, PBH-148Z; Qiagen) were used for preamplification and qPCR arrays (Rat Antibacterial Response ...
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bioRxiv - Immunology 2021Quote: ... Samples were then centrifuged and plasma isolated and stored at -20°C prior to testing in the QuantiFERON IFNγ ELISA (Qiagen). Acceptance criteria for the assay specified by manufacturers were always met.
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Microbiology 2020Quote: ... containing 1x QuantiFast SYBR Green PCR Master Mix (Qiagen), 0.2 μL of each primer (10 μM) ...
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bioRxiv - Biochemistry 2022Quote: ... containing 1,5 ml (CV) of Ni-NTA agarose (Qiagen) previously cleaned and equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... containing 2.116 units/ml QIAGEN protease (Qiagen, Cat#19155). After protease inactivation at 75°C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue pieces were collected into bead containing (Qiagen 69989) collection tubes (Qiagen 19560 ...
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bioRxiv - Immunology 2020Quote: ... 300 to 400μL of tissue-containing RLT buffer (Qiagen) were extensively vortexed for 3 minutes then centrifuged for 3 minutes (16,000rcf) ...
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bioRxiv - Microbiology 2021Quote: ... containing 1x QuantiFast SYBR Green PCR Master Mix (Qiagen), 0.2 µL of each primer (10 µM) ...
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bioRxiv - Neuroscience 2022Quote: ... containing 5 mm stainless steel beads (QIAGEN Cat#69989). To these tubes ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... B220 (RA3-6B2) and Ly-6C/G (RB6-8C5) and anti-rat IgG-coupled magnetic beads (Qiagen) as previously described [34] ...
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bioRxiv - Genetics 2020Quote: ... containing 6.25 μl 2X Fast SYBR Green Master Mix (Qiagen), 10 ng of template cDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... containing 12.5 μL of Qiagen Multiplex PCR master mix (Qiagen), 3.5 μL of 2 μM primer mix ...
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bioRxiv - Microbiology 2022Quote: ... to which RLT buffer containing Reagent DX (Qiagen, Hilden, Germany) was added ...
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bioRxiv - Microbiology 2022Quote: ... containing C1 buffer and homogeneised in a TissueLyser II (Qiagen) at 240 rpm for 2 × 1 min ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-containing aqueous phase was processed following instructions from Qiagen’s RNeasy micro kit (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: ... Reads from *.fastq files were mapped to the rat reference genome (Ensembl Rnor_5.0.78) using CLC Genomics Workbench 12.0 (Qiagen, Germantown, MD). Transcript abundance was expressed as transcript per million mapped reads (TPM ...
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bioRxiv - Developmental Biology 2023Quote: ... Reads from *.fastq files were mapped to the rat reference genome (Ensembl Rnor_5.0.78) using CLC Genomics Workbench 12.0 (Qiagen, Germantown, MD). Transcript abundance was expressed as reads per kilobase of transcript per million reads mapped (RPKM ...
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bioRxiv - Immunology 2021Quote: ... were used for preamplification and qPCR arrays (Rat Antibacterial Response, PARN-148ZE, and Human Antibacterial Response, PAHS-148ZC; Qiagen) were used for the expression analysis of 84 genes involved in innate antibacterial responses in human and rat respectively ...
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bioRxiv - Biophysics 2019Quote: ... The cells were transiently transfected with cDNA encoding the rat TRPV2 and its mutants using the Effectene reagent (Qiagen) 48-72 hours before experiments ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA was isolated from log-phase bacterial cells grown in the rat peritoneal cavity or in 2.5% NaCl HI broth using the RNeasy minikit (Qiagen). One microgram of purified RNA was converted into cDNA using QuantiTect® Reverse Transcription Kit (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: ... 1x PCR reaction buffer containing 15 mM MgCl2 (Qiagen, Hilden, Germany), 0.2 mM of each dNTP (Fermentas ...
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bioRxiv - Cell Biology 2020Quote: ... media containing cadherin was flowed through Ni-NTA agarose beads (Qiagen) packed on 1 ml Empty Bio-Scale Mini Cartridges (Biorad #7324660) ...
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bioRxiv - Neuroscience 2020Quote: ... IDT) in a tube containing 7 μL Vapor-Lock (Qiagen, 981611) to prevent evaporation ...
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bioRxiv - Microbiology 2020Quote: ... coli M15 containing plasmid pREP4 to produce the LacI repressor (Qiagen). These strains were grown in 1L LB broth at 37°C with aeration until the OD 600 nm was 0.6-1.0 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was loaded on columns containing Ni-NTA agarose (Qiagen) and incubated at 4°C on a rotator for 1.5 h (5 RPM) ...
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bioRxiv - Bioengineering 2023Quote: ... containing 3 μL 4X Probe PCR Master Mix (250102, Qiagen, USA) (final concentration 1X) ...
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bioRxiv - Immunology 2023Quote: ... Colonies containing the correct plasmid were further cultured and midipreps (Qiagen) were performed.
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bioRxiv - Molecular Biology 2023Quote: ... and 0.5 mM TCEP) containing RNase A (0.1 mg/ml, Qiagen), lysed by sonication ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.5 mM TCEP) containing RNase A (0.1 mg/ml, Qiagen), lysed by sonication ...
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bioRxiv - Bioengineering 2023Quote: ... containing 10 μL 4X Probe PCR Master Mix (250102; Qiagen, USA) (final concentration 1X) ...
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bioRxiv - Neuroscience 2020Quote: ... The gene array reaction was performed using the RT2 Profiler™ Rat Synaptic Plasticity PCR Array (Cat. No. 330231, Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Single alanine substitutions were introduced throughout the α1M2-M3 linker from L276-T283 in addition to the gain of function α1L9’T pore mutation (rat α1L263T) (QuikChange II, Qiagen). Each construct was verified by forward and reverse sequencing of the entire gene ...
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bioRxiv - Immunology 2019Quote: ... Primary mouse CD4+ T cells were purified from lymph nodes and spleens by negative selection using anti-MHCII and anti-CD8 hybridoma supernatants (M5/114 and 2.43, respectively) and anti-rat Ig magnetic beads (Qiagen BioMag). The resulting CD4+ T cells were then immediately activated on 24-well plates coated with anti-CD3 and anti-CD28 (1 ug/ml each ...