Labshake search
Citations for Qiagen :
201 - 250 of 10000+ citations for RNA Binding Motif Protein 45 RBM45 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Cancer samples were snap-frozen in liquid nitrogen and DNA extracted using the AllPrep DNA/RNA/Protein mini kit (QIAGEN, Germany) as previously reported 18 ...
-
bioRxiv - Microbiology 2019Quote: ... was used to amplify species specific 16S rRNA genes in order to identify and to quantify the relative abundance of 45 types of gut microbiota according to the manufacturer’s instructions (Qiagen, Hilden Germany) (Supplementary table 1) ...
-
bioRxiv - Cell Biology 2021Quote: Cells debris was pelleted by centrifugation at 23,000 x g for 45 minutes and the supernatant loaded onto a 10 ml Ni2+-NTA column (Qiagen, Hilden, Germany). The column was washed with 600 ml buffer A and protein batch eluted with 100 ml buffer B (buffer B = buffer A supplemented with 290 mM imidazole).
-
bioRxiv - Immunology 2022Quote: ... The lysate was then clarified by centrifugation at 16,000 x g for 45 minutes and then applied to 3 mL Ni-NTA resin (Qiagen, Valencia, CA) equilibrated with buffer A ...
-
bioRxiv - Neuroscience 2023Quote: ... After protein digestion (PK Buffer and Proteinase K (20 mg/mL) for 2 hrs at 45°C) DNA was purified with QIAquick PCR Purification Kit (Qiagen, Germany) according to manufacturer instruction ...
-
bioRxiv - Plant Biology 2023Quote: ... leaf-discs were collected and ground in autoclaved Milli-Q water using a TissueLyser II (QIAGEN; 45 seconds at 30 Hz). Serial dilutions of the ground tissue were spotted onto low salt Luria Bertani (LB ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... DNA from the head and thorax of 45 mated females was extracted using Qiagen DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). The library was prepared using the KAPA LTP Library Preparation Kit (Roche ...
-
bioRxiv - Neuroscience 2021Quote: RNA was isolated from N2a cells upon antibody treatment for 18 h using the RNeasy Mini Kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: Recombinant protein was blotted with anti-His antibody using the same method: 1:2000 mouse anti-tetra-His IgG (Qiagen); 1:1500 goat anti-mouse IgG-HRP (Dako) ...
-
bioRxiv - Cell Biology 2021Quote: ... Genomic DNA of clones showing depletion of spartin protein by immunoblot analysis with the spartin antibody were extracted (DNeasy Blood and Tissue Kit, Qiagen), and the genomic DNA sequence surrounding the target exon of spartin was amplified by PCR (sense ...
-
bioRxiv - Microbiology 2023Quote: ... and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000, Qiagen) as primary antibody ...
-
bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells treated for 48 h with 1 mM aspirin and 4 μM regorafenib using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen Cat# 80004) as per manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
-
bioRxiv - Immunology 2023Quote: Total DNA and mRNA were isolated from the intestine samples collected during fish necropsy with the AllPrep DNA/RNA/Protein Kit (Qiagen, Hilden, Germany), following manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... Individual tumors were collected in low binding DNA tubes and digested in 3 μl RLT buffer (Qiagen Cat# 1048449) for 30min at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... clarified samples were transferred to a 50 mL conical tube and supplemented with a final 20 mM imidazole pH 7.5 before batch-binding with Ni-NTA superflow resin (Qiagen) for ∼1 hr at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... The tube was subjected to bead beating for 3 x 45 seconds at 30 m/s in a TissueLyser II (Qiagen, Hilden, Germany) and placed on ice for 2 mins before repeating ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 20,000 x g for 45 minutes at 4°C and incubated with Ni-NTA agarose resin (Qiagen, catalogue number: 30230) pre-equilibrated with lysis buffer for 2 hours at 4°C ...
-
bioRxiv - Genetics 2021Quote: ... followed by protein precipitation with the Protein Precipitation Solution (Qiagen) for 10 min at -20 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... For the UCP4 antibody protein samples were extracted from the paraformaldehyde fixed tissue using the Qproteome FFPE Tissue Kit (Qiagen, Germany). The tissue blocks analyzed here were taken from the anterior cingulate and occipital cortex (as described above ...
-
bioRxiv - Biochemistry 2019Quote: ... Bound STAT3 proteins were then labeled with Alexa488-conjugated anti-6 × His antibodies (1 hr incubation, 1:20 dilution, Qiagen 35310). The array was washed and scanned using a GenePix 4400A scanner (Molecular Devices ...
-
bioRxiv - Evolutionary Biology 2021Quote: RNA-seq libraries from RNA extracted (the Qiagen plant RNA extraction kit) from two independent replicates were prepared using the TruSeq RNA sample preparation kit (Illumina Inc. ...
-
bioRxiv - Microbiology 2024Quote: ... RNA was extracted with the RNA Powersoil Total RNA kit from Qiagen.
-
bioRxiv - Neuroscience 2023Quote: ... The left hippocampus was homogenized on ice in the lysis buffer from the AllPrep® DNA/RNA/Protein Mini Kit (QIAGEN, Hilden, Germany). A sample from the lysate was used to quantify total DNA and RNA content using the appropriate Qubit® Fluorometer kit (Cat ...
-
bioRxiv - Systems Biology 2022Quote: Total RNA was isolated from whole brain tissue from 3 biological replicates per strain for 45 strains of the HRDP using QIAzol (Qiagen, Valencia, CA, USA). The brain was split sagitally and half of the brain was used for RNA sequencing ...
-
bioRxiv - Neuroscience 2019Quote: ... 200 μl of ethanol was added to the samples prior to binding to Qlamp spin column (QIAamp DNA Blood mini kit; Qiagen) and samples were centrifuged at 8000 rpm for 1 minute ...
-
bioRxiv - Systems Biology 2020Quote: ... and RNA seqFISH primary probe pools (1-10 nM per probe) and 10 nM polyT LNA oligo with a readout probe binding DNA sequence (Qiagen) were hybridized in 50% hybridization buffer consisted of 50% formamide (Invitrogen AM9342) ...
-
bioRxiv - Cell Biology 2022Quote: The heatmaps relative to the ERK-dependent AP-1 target genes were generated by interrogating the Transcription Factor Target (TFT) collection (MSigDB) and TF binding site database by QIAGEN with the top DEGs between control and PD-treated Acomys ...
-
bioRxiv - Microbiology 2023Quote: ... pH was adjusted to 8 after resuspension to allow the binding of 6x His-tagged toxins to Ni-NTA agarose resin (reference: 30210; Qiagen). Resulting samples were passed through chromatography columns containing the Ni-NTA agarose resin and were eluted with denaturing buffer containing increasing concentrations of Imidazole (10 mM ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The remaining supernatant was mixed with 13x its volume of binding buffer and DNA was purified with MinElute columns (Qiagen), following the manufacturer’s instructions with the exception of a 15-minute incubation at 37□°C during the elution step ...
-
bioRxiv - Cancer Biology 2024Quote: ... the clarified lysate was diluted into 1x Ni-NTA binding buffer and bound to equilibrated Ni-NTA resin (Qiagen, 30210) for 20 minutes at 4°C with end-over-end mixing ...
-
bioRxiv - Microbiology 2019Quote: ... RNA was stabilized with RNA protect (Qiagen), and was collected using the RNEasy kit (Qiagen) ...
-
bioRxiv - Biochemistry 2022Quote: ... Extracted RNA (Qiagen viral RNA purification kit) was eluted in nuclease-free water as per manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA extraction was performed using the RNA-easy Micro RNA extraction kit (QIAGEN) according to the manufacturer instructions ...
-
bioRxiv - Immunology 2024Quote: ... Ribosomal RNA was depleted from the total RNA using QIAseq fast select multi-RNA removal kit for mouse RNA(Qiagen) and then RNAseq libraries were made using NEB Next Ultra II Directional RNA library prep kit for Illumina ...
-
bioRxiv - Bioengineering 2021Quote: ... Protein extraction was conducted using a Qproteome Bacterial Protein Prep Kit (Qiagen)‡ ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein-protein interaction networks were built using Ingenuity Pathway Analysis (IPA) (Qiagen).
-
bioRxiv - Neuroscience 2020Quote: ... RNA was purified using RNA easy microkit (Qiagen), with the addition of DNAse ...
-
bioRxiv - Microbiology 2023Quote: ... Viral RNA extraction (QiaAmp Viral RNA kit, Qiagen) was performed using a 140 μL volume of each nasal lavage sample or virus stock ...
-
bioRxiv - Microbiology 2023Quote: Viral RNA extraction (QiaAmp Viral RNA kit, Qiagen) was performed using a 140 μL volume of each nasal lavage sample ...
-
bioRxiv - Cell Biology 2019Quote: ... Input (3% of total binding reaction) and bound samples (50% of binding reaction) were subjected to SDS-PAGE followed by immunoblot analysis with His (Qiagen 34660) and GST (BioLegend MMS-112P ...
-
bioRxiv - Genetics 2019Quote: ... The whole body of a single insect was homogenized in a PCR well containing 50 μl of the Lysis & Binding Solution and zirconia beads (ø 2 mm; Nikkato) in TissueLyser II (QIAGEN) at 25 Hz for 30 s ...
-
bioRxiv - Microbiology 2023Quote: ... we used low-binding plastics and added 1 µl of 0.1 ng/µL tRNA carrier (i.e. spike water, Qiagen, Cat. No. 1068337) in each tube with stock eluted DNA ...
-
bioRxiv - Genetics 2021Quote: ... Protein Precipitation Solution (Qiagen) was added at 0.33x and mixed well ...
-
bioRxiv - Physiology 2023Quote: RNA was extracted from human primary cells using AllPrep RNA/RNA/miRNA universal kit (Qiagen) according to manufacturer instructions ...
-
bioRxiv - Cancer Biology 2022Quote: Whole tissue protein was extracted by Qproteome Mammalian Protein Prep Kit (Qiagen, 37901) according to the manufacture’s guidelines ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated by adding 100 μL of a protein precipitation solution (Qiagen). Samples were centrifuged for 5 min at 13000 rpm ...
-
bioRxiv - Microbiology 2020Quote: ... RNA was extracted using the RNA extraction kit (Qiagen) according to the kit protocol ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was purified using RNeasy RNA isolation kit (Qiagen) and quantified and quality-assessed by Bioanalyzer ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Total RNA was extracted using QIAamp Viral RNA (Qiagen), according to manufacturer’s instructions ...