Labshake search
Citations for Qiagen :
2401 - 2450 of 10000+ citations for Protein Concentration kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... cells were lysed and RNA extracted using a Qiashredder Kit and RNeasy Plus Mini Kit (Qiagen, Cat# 796554, 74134). Purity ...
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bioRxiv - Microbiology 2020Quote: ... DNA extractions were performed using bead beating-based DNA extraction kits such as the Dneasy Powerlyzer Powersoil Kit (Qiagen) for the BeCrop® platform (patent publication number ...
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bioRxiv - Microbiology 2020Quote: ... followed by extraction of viral nucleic acids using a commercial kit (QIAamp MinElute virus spin kit, Qiagen, Venlo, Netherlands). A portion (2.5 µl ...
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bioRxiv - Microbiology 2019Quote: ... PCR clean-up was performed with UltraClean PCR Clean-Up Kit (MoBio) or UltraClean 96 PCR Cleanup Kit (Qiagen) before quantification of amplicon products with Quant-iT dsDNA Assay Kit (Thermo Fisher) ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was extracted using the PowerMax Soil DNA extraction kit (MoBio, now DNeasy PowerMax Soil kit of Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The plasmid was isolated using an endotoxin free plasmid isolation kit (EndoFree Plasmid Maxi Kit, Cat. No. 12362, QIAGEN). Before transfection THP1 cells were washed in serum free RPMI for 2 times ...
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bioRxiv - Neuroscience 2022Quote: ... total RNAs from cell pellets were isolated using the RNeasy Mini Kit or RNeasy Lipid Tissue Mini Kit (Qiagen). RNA concentration and quality were determined with Agilent 2100 Bioanalyzer System ...
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and genomic DNA was extracted using a genomic DNA purification kit (Qiagen, DNeasy Blood & Tissue Kit).
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bioRxiv - Biochemistry 2021Quote: ... Qiagen miRNeasy mini kit (217004) and Qiagen RNeasy minielute clean up kit (74204) was purchased from Qiagen (Germantown, MD). HEK293T cells (ATCC CRL-3216 ...
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bioRxiv - Genomics 2021Quote: Extractions of total RNA were performed using a commercially available kit (RNeasy Plus Mini Kit; Qiagen, Toronto, ON, Canada) according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA extraction was performed using the DNeasy Blood and Tissue Kit or the AllPrep DNA/RNA Mini Kit (Qiagen) per the manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted using the Qiagen MagAttract Power Microbiome kit DNA/RNA kit (Qiagen, catalog no. 27500-4-EP) and used for rRNA sequencing and Helicobacter spp ...
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bioRxiv - Microbiology 2021Quote: ... The pellets were re-suspended in the lyses buffer from the kit DNeasy Powerlyzer Powersoil Kit (QIAGEN, Hilden, Germany) and the DNA extracted according to the manufacturer’s procedure.
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bioRxiv - Genetics 2020Quote: ... DNA extraction from the sediment samples was performed with a commercially available extraction kit (DNAeasy PowerSoil Kit©, QIAGEN) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... or postmortem human cerebellar vermis samples using either the RNeasy Kit or RNeasy Fibrous Tissue Kit following the manufacturer’s protocols (QIAGEN). For tissue RNA extraction ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed using a Qiagen kit with the EZ1 DNA Tissue Kit following the manufacturer’s instructions (Qiagen) and DNA was eluted in a 50-μL volume [5].
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from cell pellets using a genomic DNA isolation kit (Blood & Cell Culture Midi kit (Qiagen)) ...
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bioRxiv - Immunology 2024Quote: RNA was extracted from blood or biopsies following manufacturer’s protocol using the PAXgene kit and Qiagen Rneasy Mini kit (Qiagen), respectively ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from primary mouse hepatocytes and Huh-7 cells using the RNeasy Plus Micro Kit and cDNA synthesis performed using the QuantiTect Reverse Transcription Kit according to the manufacturer’s protocol (Qiagen). Reactions were performed in triplicate in 384-well plate format ...
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CD4+ T cells regulate sickness-induced anorexia and fat wasting during a chronic parasitic infectionbioRxiv - Immunology 2022Quote: ... DNA from the blood or tissue of mice was extracted using the same kit (Qiagen blood and tissue kit) and qPCR was done using the same primers ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA removed and RNA extracted using a commercial kit (RNeasy Plus Mini Kit, Cat. 74134, Qiagen, Hilden, Germany). Sample processing for quality control and sequencing was completed by the Australian Genome Research Facility (Victoria ...
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bioRxiv - Bioengineering 2023Quote: Total RNA and cDNA were synthesized from the samples using RNeasy mini kit and QuantiTect Reverse Transcription Kit according to the manufacturer’s instructions (Qiagen). qPCR was performed in a Biorad Thermocycler with a reaction volume of 20 µl which constitutes primers (0.5 µM) ...
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bioRxiv - Cancer Biology 2023Quote: ... was isolated using Midi or Maxi kits for the validation screens gDNA was isolated using Midi kits according to the manufacturer’s protocol (Qiagen). PCR and sequencing were performed as previously described.81 ...
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bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
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bioRxiv - Microbiology 2023Quote: ... Three nucleic acid extraction kits designed for DNA and RNA extraction were compared: DNeasy PowerWater kit (QIAGEN; Hilden, Germany), NucleoSpin Soil ...
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bioRxiv - Microbiology 2024Quote: ... and RNA was extracted following the manufacturers instructions (Zymo Quick-RNATM Viral Kit or QIAGEN RNeasy Mini Kit, respectively).
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bioRxiv - Biophysics 2020Quote: ... proteins were expressed in Escherichia coli strain BL21 (DE3) and purified via nickel-nitrilotriacetic acid affinity chromatography (Qiagen) followed by ion exchange chromatography on an Äkta system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: Purified recombinant poly-histidine protein was obtained by FPLC under native conditions using a Ni-NTA column (Qiagen), and a Biologic DUO-FLOW BIO-RAD chromatography system (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... microdissected human brain tissue was flash frozen and lysed to extract proteins using a TissueLyser II system (QIAGEN) and total protein concentration determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific 23225) ...
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bioRxiv - Molecular Biology 2021Quote: ... The expressed EcoCas3-tag fusion proteins were purified using nickel affinity resin (Ni-NTA, Qiagen, Hilden, Düsseldorf, Germany). To remove tags ...
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bioRxiv - Molecular Biology 2019Quote: ... coli cells grown at 37°C by addition of 0.2 mM IPTG and native protein purified using Nickel affinity chromatography according to the manufacturer’s protocol (Qiagen). For N-terminal His-tag removal ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were purified from the cell lysate by affinity chromatography using a Nickel-NTA column (Qiagen, Valencia, CA). A solution containing 20mM HEPES pH7.2 ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then purified on Ni2+ -NTA affinity resin following a modified version of the manufacturer’s instructions (QIAGEN) [25] ...
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bioRxiv - Microbiology 2020Quote: ... The 6 × his-tagged proteins were purified using a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix (30250, Qiagen). Cell-free extracts were loaded on 0.5 ml Ni-NTA matrixes and incubated on a roller shaker for 2 h at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged proteins were purified by Ni-affinity gravity-flow chromatography using the Ni-NTA agarose (Qiagen) following the product manual.
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the tags and protease were removed from the protein by passage through a Ni-NTA superflow cartridge (QIAGEN). The protein was further purified by size exclusion chromatography on a Superdex 200 Increase (Cytiva ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed on the eluted DNA samples at 50°C for 30 minutes (Qiagen Protease mix). ChIP DNA was purified using Sera-Mag beads (Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... The lysates were clarified by centrifugation and proteins in the supernatant were purified using Ni-NTA chromatography (Qiagen) for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... The protein prokaryotic expression and purification was according to the instruction of Ni-NTA Agarose (Cat.No.30210, QIAGEN) and Glutathione Sepharose (GE).
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bioRxiv - Bioengineering 2020Quote: Initial small test purifications were performed using Ni-NTA Spin Column for His-Tagged proteins (Qiagen, Hilden, Germany) following the protocol for 6xHis-Tagged Proteins under Native Conditions from E ...
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bioRxiv - Biochemistry 2020Quote: ... The Ni-NTA agarose resin for purification of his-tagged proteins was purchased from Qiagen (Germantown, MD, USA). Superdex 75 16/600 and Superdex 200 Increase 10/300 size exclusion chromatography columns were purchased from GE Healthcare (Uppsala ...
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bioRxiv - Immunology 2021Quote: ... to provide Uniprot entries and further processed in Perseus (v1.6.6.1).18–20 Significantly regulated proteins were investigated in Ingenuity Pathway Analysis (v47547484, Qiagen, USA)
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bioRxiv - Developmental Biology 2021Quote: Log fold-change values of the top 5% proteins were used as input for Ingenuity Pathway Analysis (Qiagen: https://digitalinsights.qiagen.com/products/features/) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were disrupted by sonication and N-terminus His6-tagged proteins were purified on Ni-NTA columns (Qiagen) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins NHis6-Fnr1 and Fnr3-CHis6 in the supernatant were purified on Ni2-NTA resin (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and the cleaved His6-MBP tag was removed by passing the protein through an amylose resin column (Qiagen). The flow-through fraction containing the tag-free protein was further purified using a Superdex 200 (10/300 GL ...