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Citations for Qiagen :
2351 - 2400 of 4234 citations for 6 chloro 2 N 2 N diethyl 4 N propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2021Quote: ... 0.1 μL of 5 U/μL Taq DNA Polymerase (QIAGEN, Valencia, CA, USA), 0.2 μL of U19 fluorescent dye ...
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bioRxiv - Immunology 2020Quote: ... miRNA inhibitors (miRCURY LNA miRNA power inhibitor with or without 5’-FAM, Qiagen) were added directly to culture media at the time of activation and were taken up by the cells by spontaneous translocation across the cell membrane ...
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bioRxiv - Pathology 2019Quote: ... with sterile 5 mm stainless steel beads and processed using Tissuelyzer LT (Qiagen). The homogenate was lyzed with proteinase K and subjected to DNA extraction using the QIAmp DNA mini kit (Qiagen) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied to three 5 ml Ni-NTA Superflow cartridges (Qiagen). The resin was washed with 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to a 5 ml Ni-NTA Superflow cartridge (Qiagen) in order to remove the uncleaved fusion protein and His6-TEV protease ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Genetics 2021Quote: ... The supernatant was treated for 5 minutes at room temperature with RNAse (Qiagen) and then centrifuged again ...
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bioRxiv - Bioengineering 2021Quote: ... total RNA was extracted from 5 organoids using the RNeasy Plus MiniKit (Qiagen). Purity was determined byA260–A280 ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from 5 ml samples using the miRNAeasy mini kit (Qiagen), treated with TURBO DNase (Ambion ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg/mL DNase I (AppliChem) and 200 µg/mL RNase A (Qiagen) using 4 cycles of 15 s sonification at 30% duty cycle and 30% output control ...
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bioRxiv - Molecular Biology 2024Quote: ... The dialysate was loaded onto 5 ml Ni2+-NTA column (Qiagen, Valencia, CA). After washing the column with buffer A containing 20 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was mixed with 5 ml of Ni-NTA agarose (QIAGEN) pre-equilibrated with solubilization buffer for 60 min ...
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bioRxiv - Microbiology 2024Quote: ... 37°C in 5% CO2 and purified with a RNeasy mini kit (Qiagen) according to the protocol for Gram-positive bacteria ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was loaded onto a 5 mL StrepTactin Superflow Plus catridge (Qiagen) using a 1 mL/min flow rate and washed with Cyclin B1 wash buffer and eluted with wash buffer containing 2 mM desthiobiotin ...
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bioRxiv - Molecular Biology 2023Quote: mRNA was extracted from ∼5×106 cells using the miRNeasy Mini Kit (QIAGEN). Library preparation and sequencing was conducted by City of Hope Integrative Genomics Core ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA from 5×107 cells was extracted using RNeasy mini kit (Qiagen). One μg total RNA was used for cDNA synthesis using Quantinova Reverse Transcription kit (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples are next loaded onto a 5 mL Hitrap S column (Qiagen) pre-equilibrated with 50 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... homogenized using 5-mm steel beads and a tissue lyser (Qiagen TissueLyser II) 6x at 25 Hz for 15s ...
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bioRxiv - Cancer Biology 2022Quote: A 100 µL slurry of 5% Ni-NTA magnetic beads (Qiagen, part# 36113) was placed in 12 1.5 mL Eppendorf tubes and the tubes were placed in a magnetic stand and the supernatant was removed ...
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bioRxiv - Microbiology 2022Quote: ... (5) hybrid libraries were then eluted in 40μL of elution buffer (Qiagen, 19086).
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bioRxiv - Microbiology 2023Quote: ... or a 1.5 ml tube with 5 volumes of RNAlater (Qiagen, Valencia, CA) and preserved at -80 °C for RT-PCR.
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bioRxiv - Microbiology 2023Quote: ... containing 500 µl PBS and one 5 mm steel bead (Qiagen, Hilden, Germany) prior to bead-beating for 20 seconds to disrupt the cuticle ...
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bioRxiv - Physiology 2024Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Germany). Reverse transcription was initiated with a hold at 50 °C for 10 min (cDNA synthesis) ...
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bioRxiv - Molecular Biology 2024Quote: Gcn4 and Med15 supernatants were mixed with 5 ml Ni-NTA beads (Qiagen) and incubated at 4°C for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was applied to 5 ml of Ni-NTA agarose beads (Qiagen). The beads were washed with 200 ml of wash buffer (50 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2024Quote: ... and TY1C2 were mixed with 5 μg/ml of RNase A (19101, QIAGEN) and 1 mg/ml of Proteinase K (EO0491 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... total RNA and genomic DNA was extracted from flash frozen testes of a subsample of males 6 to 10 days following injection with the dsRNA using a Qiagen Allprep DNA/RNA Mini Kit (Qiagen, Venlo, The Netherlands). Expression levels for Dnmt1 was analyzed using qRT-PCR as described above.
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bioRxiv - Plant Biology 2020Quote: ... and whole seedling shoot of 8-day-old seedling (Figure 2C and Supplemental Figure 6) were used to extract RNA using the RNeasy® Mini kit (Qiagen, Hilden, Germany) followed by DNase I (Qiagen ...
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bioRxiv - Cancer Biology 2019Quote: Cells were seeded in 6-well plates 24 h prior to isolation of total RNA using a RNeasy Mini Kit (QIAGEN, Santa Clara, CA). mRNA levels for EMT-related genes were determined using the validated primer sets (SA Biosciences Corporation ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from 3 months old Pgrcre and FOXL2OE diestrus uteri using RNeasy mini kit (Qiagen). The library was prepared using TruSeq RNA Library Prep kit (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... total cfDNA was extracted from 3 mL of separated plasma using the QIAamp Circulating Nucleic Acid kit (QIAGEN) following manufacturer instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from 3-day-old seedlings using a QIAshredder and RNeasy Plus Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 1000 µL PBS was added to each sample (lungs, 0.01–0.04 g) along with Tungsten carbide 3 mm beads (Qiagen). Samples were homogenized at a speed of 10 Hz for 10 min and then centrifuged at 15,000 × g for 10 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were then ground to powder using a 3 mm tungsten carbide bead (Qiagen Cat. No. / ID: 69997) on a TissueLyser II (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... homogenized in sterile 0.05 % NP40 in H2O for 3 minutes at 25 Hz using a Tissue Lyzer (Qiagen) and serial dilutions were plated on YPD agar containing 100 μg/ml Ampicillin ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were transfected at 3 DIV with siRNA targeting Kdm6a or ntRNA using Effectene Transfection Reagent (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 500 µl of PBS-AB was added to each sample along with Tungsten carbide 3 mm beads (Qiagen). Samples were homogenized during 10 min and then centrifuged at 15,000 g for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed 3 times with PBS and lifted with 500µL/well of Cell Protect Reagent (Qiagen) for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... benthamiana leaves were grinded in a tube with two glass beads (3 mm) with a TissueLyser II (Qiagen) and directly after supplied with 600 μl EB ...
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bioRxiv - Microbiology 2019Quote: ... RNA samples from 3 independent cultures for each strain (Chr_dam and Chr_gfp) were extracted with RNeasy miniprep kit (Qiagen). Primers used are listed in Table S1 ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from an aliquot of 3 x 106 cells using the RNeasy Plus Kit (Qiagen). cDNA was synthesized from extracted RNA using the iScript cDNA Synthesis Kit (BioRad ...
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bioRxiv - Microbiology 2023Quote: RT-qPCR was performed in an Applied Biosystems QuantStudio 3 using the QuantiTect® Multiplex PCR Kit (QIAGEN). The primers and probes used are listed in Table S2 ...
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bioRxiv - Genomics 2024Quote: ... with two 3 mm metal beads and ground into a fine powder with a TissueLyser II (Qiagen, Germany) at 30 Hz for 2 min ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was isolated from HEK293T cells 3 days after transfections using the Gentra Puregene Cell Kit (Qiagen) and was diluted to 10 ng/μL ...
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bioRxiv - Molecular Biology 2022Quote: ... washed 3 times with PBS and had their genomic DNA extracted with the QIAamp DNA mini kit (QIAGEN). Sequencing libraries were prepared at GenomeScan (Netherlands ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was isolated from 0 or 3% CSE-treated bronchospheres using the Qiagen RNeasy Mini Kit (Qiagen 74106). The RNA yield was measured using Agilent TapeStation (4200 TapeStation System ...
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bioRxiv - Microbiology 2023Quote: ... Individual mosquitoes were homogenized for 3 minutes at a 30Hz/s frequency in a TissueLyser II grinder (Qiagen). DNA extraction of individual larvae was carried out using the QIAamp DNA Kit (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was extracted from 3-week-old plants using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: siRNA targeting human GPRC5A (siGPRC5A_4 against target sequence 5′-CTGGGTGTGTTGGGCATCTTT-3′, cat# SI04438021) and non-targeting control siRNA (cat# Ctrl_AllStars_1, target sequence not disclosed) were purchased from Qiagen. Human primary keratinocytes were transfected at 20 nM final siRNA concentration using Lipofectamin 2000 reagent (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from 3 dpf zebrafish AB larvae (50 fish) using RNAeasy Plus Kit (Qiagen 74134). cDNA was synthesized using SuperScript IV Reverse Transcriptase (Invitrogen) ...