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Citations for Qiagen :
2351 - 2400 of 3581 citations for 6 Methyl 3 4 dihydro 2H pyrido 3 2 b 1 4 oxazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: ... Microsatellites were co-amplified in multiplex PCR (Table 2) with a Thermocycler T Gradient machine (Biometra, Goettingen, Germany) using the Qiagen® Multiplex PCR Kit (Qiagen, Hilden, Germany). Forward primers were 5’-labeled with fluorescent dyes HEX ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA extraction was performed from 25 mg (±2 mg) of arthropod powder with Qiagen Dneasy® Blood & Tissue extraction kit (Qiagen, Hilden – Germany) following the manufacturer’s protocol (see Sire et al ...
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bioRxiv - Genetics 2024Quote: ... PCR products were run in a 2% agarose gel and the correct band (around 200 bp) was purified with QiaQuick gel extraction Kit (QIAGEN, catalog number 28706) (at least 2 columns ...
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bioRxiv - Plant Biology 2024Quote: ... Tissue samples were ground by shaking with glass beads in a TissueLyser II (Qiagen, 2 pulses of 30 s each at 28Hz shaking). Leaf powder was suspended in 1.2 ml of cold nuclear isolation buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... and a plasmid expressing VSV-G or HIVKB9 envelope glycoproteins were cotransfected at the mass ratio of 9:1 (9 Hi.fate / 1 Env) using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... or WT-Cav-1 (1 µg) plus EphB1-Y600F-YFP (2.5 µg using Superfect transfection reagent (cat #301305, Qiagen). Media was replaced 6 h after transfection with fresh DMEM media containing 10% FBS ...
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bioRxiv - Physiology 2020Quote: ... insulin-like growth factor 1 receptor (Igf1r) and sirtuin 1 (Sirt1) gene promoters were designed using the Pyromark Assay Deisgn 2.0 software (Qiagen). PCR and sequencing primers are provided in Supplementary Table 1 ...
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bioRxiv - Plant Biology 2022Quote: RNA was extracted from 10-day-old whole seedlings grown on 1/2X MS media containing 1% sucrose and 0.8% agar (Plant RNeasy kit (Qiagen)) ...
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bioRxiv - Systems Biology 2023Quote: ... x mg solid matrix were mixed with five times the μl amount of ACN:water (1:1, v/v) and homogenised with a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After a short centrifugation (2 min ...
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bioRxiv - Molecular Biology 2021Quote: ... or 1 μl 1:10 diluted oligonucleotides (for RNA from immunopurified ribosomes) from the QIAseq FastSelect –rRNA Yeast Kit (Qiagen) in 10.5 μl total at 75°C ...
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from circulating PD-1+ and PD-1− CD8+ T cells using the All-Prep DNA/RNA Micro Kit from Qiagen and sent to Adaptive Biotechnologies for survey-level TCRβ sequencing.
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bioRxiv - Microbiology 2019Quote: ... A second round of amplification was performed by transferring 5µl of 1:100 diluted Round 1 product into 15µl Fast Cycling PCR Mix (QIAGEN, Hilden, Germany) reactions ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from FACS purified Zeb1+ (Lineage−Sca-1+CD49fhi tdTomato+) or Zeb1− basal cells (Lineage−Sca-1+CD49fhitdTomato−) using a RNeasy micro kit (Qiagen). cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara) ...
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bioRxiv - Cell Biology 2019Quote: ... Lentivirus carrying the response elements for type I (ISRE - #CLS-008L-1) or type II (GAS - #CLS-009L-1) upstream of firefly luciferase was purchased from Qiagen. Twenty-four hours post plating ...
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bioRxiv - Neuroscience 2020Quote: ... at 60°C overnight and 1 μl of a 1:20 dilution of the lysate used in a PCR reaction (HotStarTaq, Qiagen). The sequence flanking the tmt-opsin1b TALEN binding sites was amplified using the forward 5’-GGGACTTTCTTTGCGCTTTA-3’ and the reverse 5’-CAGGTCAGAGCGGATCTCAT-3’ primers ...
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bioRxiv - Microbiology 2021Quote: ... qPCR reactions contained 4 μl of cDNA (1:50 dilution) and 200 nM of each primer (supplementary table 1) and QuantiTect SYBR Green PCR master mix (Qiagen) or SensiFAST SYBR Hi-ROX kit (Bioline) ...
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bioRxiv - Bioengineering 2019Quote: ... the cells were washed once with 0.1% PBSA and incubated with 50 μL of a 1:100 dilution of anti-penta-His Alexa 647 antibody (Qiagen) for 10 minutes on ice ...
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bioRxiv - Immunology 2019Quote: ... or M-MØ (1×106 cells/well) were transfected with 1 μg of the XRE reporter construct using either Superfect (Qiagen) or VIROMER RED (Lipocalix) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with a 1:1 molar ratio of eGFP-P2A-Cas9 and single stranded oligonucleotide using Effectine (Qiagen) in a six-well dish ...
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bioRxiv - Biochemistry 2020Quote: ... The restriction digestion efficiency was validated using 1% 1× TBE agarose gel electrophoresis and purified with a QIAquick PCR Purification Kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... homogenized in 1 ml PBS by bead beating (3mm steel ball, 25 Hz for 1 minute in a TissueLyser (Qiagen)) ...
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bioRxiv - Genomics 2021Quote: ... 1 g of ground leaf powder was resuspended in lysis buffer with 1 mg/ml of proteinase K (19133, Qiagen) and 190 μg/ml of RNase A (19101 ...
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bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from 1×106 MCF7 and 1×106 BT-474 cells (‘bulk’) using the RNeasy Mini Kit (74104, Qiagen) in combination with the QIAshredder (79654 ...
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bioRxiv - Immunology 2022Quote: ... For bacterial load determination one lobe of the lung was collected in 1 mL PBS with 1 metal bead and smashed using a tissue lyser (Qiagen). 25 μL of lysed lung were put in duplicates on CYE-plates and grown for 3-4 days at 37°C in a non-CO2 incubator.
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bioRxiv - Physiology 2024Quote: ... After chloroform incubation and centrifugation per manufacture instructions RNA-containing phase was diluted with 70% ethanol 1:1 and RNA isolation was performed using RNeasy Mini Kit (Qiagen). For tissues ...
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bioRxiv - Microbiology 2023Quote: ... was added to the supernatant to give a final concentration of 1 mM and the resultant suspension was applied to a 1 ml Ni2+-NTA column (Qiagen). The resin was then washed sequentially with 10 ml of lysis buffer (without Triton X-100) ...
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bioRxiv - Cell Biology 2023Quote: ... The aqueous phase was mixed 1:1 with 70% ethanol and applied to a minikit spin column and washed with RW1 and RPE buffer (Qiagen). cDNA was synthesized using the QuantiTect Rev ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
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bioRxiv - Plant Biology 2023Quote: ... of g1090::XVE>>AXR3-1-mCherry and HS::AXR3-1 were extracted following the protocol (Plant Total RNA Mini Kit, QIAGEN). g1090::XVE>>AXR3-1-mCherry were transferred for 2 h on estradiol-containing medium and then harvested ...
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bioRxiv - Microbiology 2023Quote: ... A volume of 1:1 of 70% ethanol was mixed with the upper phase before transfer on column (RNeasy Mini kit, Qiagen), and RNA was extracted according to manufacturer directions ...
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bioRxiv - Bioengineering 2022Quote: ... the aqueous phase was mixed with ethanol in a 1:1 ratio and was added to RNeasy spin columns (Qiagen) for RNA purification ...
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bioRxiv - Cancer Biology 2023Quote: ... Digested PCR fragments were ligated into the restricted vector at a 1:1 molar ratio using T4 DNA ligase and were purified using the MiniElute PCR purification kit (Qiagen). Purified ligated plasmids were electroporated into MegaX DH10B (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... The significantly differentially expressed genes with a log2 fold change <= −1 or >= 1 were input into Ingenuity Pathway Analysis (Qiagen) to determine pathway enrichment (significance determined by Fischer’s exact method).
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bioRxiv - Molecular Biology 2024Quote: ... fungal DNA was extracted independently from 1 mL of crude conidial suspensions (between 108 and 109 spores mL-1) using the DNeasy 96 Plant Kit (Qiagen) following the manufacturer’s instructions (Gautier et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in Propidium Iodide (PI) staining solution (1x PBS supplemented with 50 µg/mL PI, Invitrogen; 100 µg/mL RNase A, Qiagen; and 2 mM MgCl2) and incubated for 30 min at room temperature in the dark) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 ng of pFLAG expression vectors or empty plasmids and 50 ng psiCheck-2 plasmid were transfected using Effectene transfection reagent (Qiagen, Netherlands, Cat. No. 301425), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions were quenched at different time points (2, 5, 7, 10 and 20 minutes) by the addition of 250 μL of PB buffer (Qiagen QIAquick PCR Purification Kit) supplemented with 10 mM of EGTA ...
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bioRxiv - Genomics 2020Quote: ... 80 µl of Viral Transport Media that had previously stored a nasopharyngeal swab from a patient infected with SARS-CoV-2 were used for RNA isolation using the QIAamp Viral RNA Mini spin kit (Qiagen, Cat No./ID: 52904) according to manufacturer specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Both the input and IP samples were incubated at 50°C for 2 hr and then cleaned up using the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany, Cat # 28104) and eluted in 35 μL of water.
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bioRxiv - Systems Biology 2019Quote: RNA was extracted from a total of 17 samples from Experiment 2 with the AllPrep PowerFecal DNA/RNA Kit (Qiagen USA, Cat. No. 80244). The 17 samples included 7 target samples taken during antibiotic treatment (4 untreated and 3 non-responder ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the remaining 2 mL of the cell suspension using the RNeasy® Protect Bacteria Mini Kit (QIAGEN, Cat. No. 74524) following the manufacturer instructions.
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from bulk HSPC samples (∼2 x 106 cells per sample) using a QIAGEN Blood & Tissue DNA isolation kit (QIAGEN, Inc., Germantown, MD, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... and 0.5 mg.ml-1 of Proteinase K (Qiagen), to reverse formaldehyde crosslinking ...
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bioRxiv - Microbiology 2019Quote: ... 1 ml RLT buffer (Qiagen RNA Isolation Kit) + 10 μl β-mercaptoethanol was added and vortexed and a phenol chloroform extraction followed by an ethanol precipitation was carried out ...
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bioRxiv - Microbiology 2021Quote: ... and 1-5mL Qiazol Lysis Reagent (Qiagen, #79306) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... amplification grade (1 U/µg RNA, Qiagen, #79254). Real-time qPCR was performed with a MyiQ™ instrument (BIO-RAD) ...
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bioRxiv - Biochemistry 2022Quote: ... Penta-His Antibody (Qiagen, 34660, IB: 1:1000) and Y188 to APP C-terminus (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... with on-column DNase-1 digestion (79254, Qiagen). Purified RNA had 260/280 ratios ~2 (via Nanodrop 2000c ...
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bioRxiv - Neuroscience 2019Quote: ... 5nmol of Rho-1 siRNA (Qiagen, Germany #SI01401743) was diluted in rnase-free water (provided in kit ...