Labshake search
Citations for Qiagen :
2251 - 2300 of 10000+ citations for TBARS MDA Universal Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The RNA extraction process was referred to the instructions of the kit (RNeasy Mini kit, QIAGEN, Germany). The extracted RNA was detected by ultra-fine ultraviolet spectrophotometer (NanoDrop ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR product was purified by either QIAquick Gel Extraction Kit or QIAquick PCR Purification Kit (Qiagen), cloned into the donor vector pDONR207 using Gateway BP Clonase (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was purified with RNeasy plus micro kit or RNeasy plus mini kit (Qiagen, Hilden, Dermany). Complementary DNA was synthesized by a SuperScript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was purified using the Xtreme DNA isolation kit (Isohelix) or DNeasy Blood & Tissue Kit (Qiagen). These kits were used to purify DNA from crude lysates or from tissue samples preserved in RNAlater solution (Sigma).
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bioRxiv - Microbiology 2021Quote: ... and viral DNA was extracted and purified using a commercial kit (DNeasy Blood and Tissue Kit, Qiagen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Amplified scFv PCR fragments were purified with Gel Extraction kit (Qiaquick Gel Extraction Kit, Cat# 28706, QIAGEN), and subjected to SfiI (Fast Digest SfiI ...
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bioRxiv - Microbiology 2022Quote: gDNA was purified from Bacteroidales using a DNeasy UltraClean Microbial Kit or DNeasy Blood & Tissue Kit (Qiagen). Up to 2 μg in 200 μL TE buffer was sheared by ultrasonication to an average of 3–5 kb with a Covaris S220 focused ultrasonicator using red miniTUBEs ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNAs was further isolated by using the Qiagen FFPE RNA extraction kit (RNeasy FFPE kit, Qiagen) for the discovery set and the Recover AllTM Total Nucleic Acid Isolation Kit for the French external validation cohort (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted using the NucleoSpin RNA Mini kit (Machenery Nagel) or the RNeasy Mini Kit (Qiagen). 1-2 ug of total RNA was DNaseI (Ambion ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested and total RNA isolated using an RNA isolation kit (RNeasy Mini kit, Qiagen, 74106). 8 µg of total RNA were treated with DNaseI and purified according to the manufacturer’s protocol (TURBO DNA-free kit ...
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bioRxiv - Microbiology 2024Quote: ... an EZ1 extraction was performed using a Qiagen kit with EZ1 DNA Tissue Kit (Qiagen, Courtaboeuf, France) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... Fig S1B) using the Qiagen RNeasy mini kit (RNeasy Mini Kit, Cat No. 74104, Qiagen, Hilden, Germany) ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted using DNAeasy Power Soil Pro kit or DNAeasy PowerMax Soil kit (Qiagen, Kista, Sweden) for 47 mm and 142 mm filters ...
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bioRxiv - Microbiology 2023Quote: ... #342746 and #343061 using different DNA extraction kits: DNeasy Blood and Tissue kit (Qiagen GmbH, Hilden, Germany), and NucleoSpin Virus (Macherey-Nagel ...
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bioRxiv - Bioengineering 2023Quote: ... coli and purified for cell culture application through commercially available kits (Qiagen, Maxi Plasmid Kit, Cat# 12162).
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bioRxiv - Microbiology 2023Quote: ... We compared the extraction kits QIAamp UCP Pathogen Mini Kit (Cat No. 50214, Qiagen, Hilden, Germany; ‘Q’) versus ZymoBIOMICS DNA Microprep Kit (Cat No ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total DNA was isolated from cells adherent cultures by Genomic Mini Kit (Qiagen Dneasy blood & tissue kit) according to the producer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... before lysed using RLT buffer supplied in the RNeasy Mini Kit (Qiagen extraction kit, 74104, Qiagen, Germany). RNAs were collected by following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... before lysed using RLT buffer supplied in the RNeasy Mini Kit (Qiagen extraction kit, 74104, Qiagen, Germany). RNAs were collected by following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA from single colonies was extracted with the QIAprep Spin Miniprep Kit and Maxi Kits (Qiagen) and confirmed by Sanger sequencing (Genewiz ...
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bioRxiv - Cell Biology 2024Quote: ... genetic DNA was isolated from the mouse tails using a DNA isolation kit (QIAGEN DNA isolation kit). Genotyping of Hif2α mice was determined by PCR amplification ...
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bioRxiv - Neuroscience 2019Quote: ... Relative gene expression was measured using RT2 Profiler low-density PCR plates preloaded with qPCR primer assays for genes encoding GABA- and glutamate-related targets (PN: PARN-152ZA, Qiagen). Thermal cycling and data collection were accomplished using an ABI Real-Time PCR 7300.
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bioRxiv - Genomics 2019Quote: ... We used a 100 µm nozzle to sort single cells into 96-well plates containing 5 µl TCL buffer (Qiagen) with 1% beta-mercaptoethanol for Smart-seq2 and 384-well plates containing 0.6 µl 1% NP40 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... 1.2×106 cells were plated into each well of a 6-well plate and transfected with Sns and Eff-1 constructs (200ng each) using Effectene (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... followed by flow sorting with a Sony SH800Z (gating on calcein NVOC fluorescence levels) into individual wells of a 96-well plate containing 5 μL of Buffer RLT (Qiagen) and 1% β-mercaptoethanol.
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bioRxiv - Biochemistry 2020Quote: ... 5.6 x 105 cells were seeded in 4 ml DMEM in a 6-cm culture plate and transfected with targeting or control siRNA (Table 4) (from Qiagen) to a final concentration of 20 nM for each siRNA used ...
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bioRxiv - Systems Biology 2021Quote: ... Crystal screening was performed with a Gryphon robot (Art Robbins instruments, Sunnyvale, USA) using sitting-drop 96-well plates and Screening Suites (Qiagen). Optimization experiments were set up in 24-well hanging drop crystallization plates ...
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bioRxiv - Cancer Biology 2020Quote: ... Our panel of 88 target genes was prepared on a 96-well plate format with housekeeping genes and controls (RT2 PCR array, Qiagen). Expression was quantified on an ABI7500 real-time PCR machine (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: Single cells were sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, Qiagen 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Individual females were homogenized in QIAGEN ATL Buffer in 96 well-plates with a sterile glass bead per well on a Tissue Lyser II (QIAGEN) at 23s/f for 2 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... 2i/LN511 or PDMK/LN511 conditioned cells at 70% confluency in a well of a 6-well plate using RNeasy Plus Mini (Qiagen). Three biological replicates were prepared ...
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bioRxiv - Molecular Biology 2020Quote: ... catalysis was measured using a previously described assay (22) in which hexahistidine-tagged PARP-1 was immobilized on Ni(II)-NTA-coated plates (Qiagen) in the presence of NAD+/biotinylated-NAD+ (Trevigen) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
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bioRxiv - Cancer Biology 2020Quote: ... we captured poly-adenylated mRNA from cell lysates using a 96-well plate with oligo(dT) grafted to the inner walls of each well (Qiagen). Next ...
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bioRxiv - Genetics 2019Quote: ... S2 and KC cell lines were stably transduced with the pMKInt-Hyg vector encoding RasV12Common and RasV12Rare cDNAs using 1000 ng of DNA in 6 well plates as instructed by Effectene transfection reagent (Qiagen). The following day ...
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bioRxiv - Genomics 2021Quote: ... were sorted into individual wells of a 96 well plate containing lysis buffer 2.5μL RLT Plus Lysis Buffer (QIAGEN, catalog # 1053393) with 1U/μL SUPERase-In (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... single MR1-tetramer-binding cells from Peruvian participant 7-3 and blood bank donors 702A and 703A were sorted into 96-well plate coated with Vapor-Lock (Qiagen) containing Iscript cDNA synthesis mixture (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: ... Converted samples were randomized in a 96-well plate and sequenced for each CpG on PyroMark Q96 ID provided by QIAGEN. The raw data were processed in PyroMark Q24 Software v2.5.8 by QIAGEN.
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and CD45+ Calcein+ DAPI-CD19+ CD38+ activated B cells were single-cell sorted into 96-well plates with catching buffer (RLT lysis buffer (Qiagen) with 1% 2-mercaptoethanol (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2020Quote: ... 5 μl of amplified cDNA from each well of a 96-well plate were pooled and completed to 500 μl with PCR-grade H2O (Qiagen). Two rounds of 0.6X solid-phase reversible immobilization beads (AmpureXP ...
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bioRxiv - Immunology 2021Quote: ... and subsequent crystallization optimization was performed manually using hanging-drop vapor diffusion method with 15-well screw-cap crystallization plates (Qiagen). The H5.28-HA head complex was crystallized in 7-9% PEG 8000 ...
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bioRxiv - Immunology 2021Quote: Single cells were index-sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, QIAGEN 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: HMECs were transfected with 30 nM miRNA or 30 nM antigomiR (anti-miRNA) in 384-well plates using HiperFect (Qiagen), and the protocol described above for ‘High-content miRNA Screening’ was followed ...
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bioRxiv - Neuroscience 2022Quote: ... iPS-NES cells were plated in 6-well plates and lysates were collected in RNAprotect® Cell Reagent (QIAGEN, #76526) to stabilize the RNA ...
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bioRxiv - Plant Biology 2022Quote: Leaf tissue samples were collected and freeze-dried in a deep-well plate and ground in a TissueLyser II (QIAGEN) for 4-6 minutes at a frequency of 25 Hz ...
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bioRxiv - Biochemistry 2023Quote: A kinome-wide siRNA library that contained 4 individually arrayed siRNA sequences in 384-well plates was purchased from Qiagen. The library consisted of known kinases and associated proteins ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 96-well plates containing lysis buffer with proteinase K solution in PKD buffer (1:16) (Qiagen) and were stored at -80ºC ...
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bioRxiv - Neuroscience 2023Quote: ... and colonies yielding an ≈2.4 kB product were isolated from a duplicate patch plate and grown in liquid culture as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Molecular Biology 2023Quote: Primary monocytes were cultured in 24-well plates and then transfected with control non-targeting siRNA (Dharmacon) or siRNA targeting ERN1 (Dharmacon) using HiPerFect reagent (QIAGEN) following the instructions provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...