Labshake search
Citations for Qiagen :
2251 - 2300 of 4402 citations for 6H 1 3 Thiazin 6 one 2 ethoxy 4 hydroxy 5 nitro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: 4.5×106 - 7.3×106 live control or oxPAPC-treated Tregs were harvested on day 3 of culture and RNA was isolated using RNeasy Plus Mini Kit (Qiagen Cat #: 74134) according to manufacturer instruction ...
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bioRxiv - Evolutionary Biology 2022Quote: RNA was isolated from abdomens from 3 days post injection pupae collected during the experimental infection experiments using the RNeasy Mini Kit (Qiagen, Hilden, Germany) following manufacturers’ protocol including a DNAse incubation step and quantified using a Nanodrop ...
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bioRxiv - Cancer Biology 2023Quote: ... BRD4-S (targeting short variant 3’-UTR region; Cat#: SI05044865) and AllStars Negative control (Cat. #: SI03650318) were purchased from QIAGEN (MD, USA). ITGA4 siRNA (Cat ...
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bioRxiv - Neuroscience 2024Quote: ... PCR products were run ona 3% agarose gel to recover of the NGS amplicon libraries and purified by MinElute Gel Extraction Kit (QIAGEN, Germantown, MA). Libraries were sequenced on an Illumina Novaseq 6000 using a 350-cycle reagent kit and paired end read chemistry yielding 175 bp paired end read outputs ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested by centrifugation at 4°C followed by cell lysis/RNA extraction using RNEasy plus mini kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA of CDC-EVs (n=12) and MSC-EVs (n=4) was extracted using the miRNeasy Serum/Plasma kit (QIAGEN). Library construction was performed according to the manufacturer’s protocol using the TruSeq small RNA Library Kit (Illumina) ...
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bioRxiv - Cell Biology 2020Quote: ... lysed by sonication and the lysates were centrifuged at 52000 g for 45 min at 4°C.The supernatants were incubated with Ni-NTA agarose resin (Qiagen, Germany) for 2 hours before washing with 100 bed volume buffer A supplemented with 50mM imidazole ...
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bioRxiv - Genetics 2021Quote: ... 12 WT littermates) and two-month-old mice (adult, n = 4 per group) were lysed and homogenized in QIAzol Lysis Reagent (Qiagen). Total RNA purification was performed with the miRNeasy Micro Kit (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... PCRs were performed on 4 ng of the same DNA used for long-read sequencing and using the 2X HotStartTaq Master Mix (Qiagen). PCR products were checked by agarose gel electrophoresis and purified using Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) ...
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bioRxiv - Genomics 2020Quote: ... We stored tissue at 4 °C for 24-72 hours prior to extracting DNA with a DNAeasy Blood and Tissue Kit (Qiagen) with on-column RNase A treatment following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The insoluble fraction was precipitated by ultracentrifugation (20,000 g) for 30 minutes at 4°C and the supernatant was loaded onto a Ni-NTA superflow affinity column (QIAGEN). The Ni-column was then wash three times and eluted with buffer containing 30 mM HEPES (pH 7.8) ...
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bioRxiv - Biochemistry 2021Quote: ... the cell lysate was cleared by centrifugation (20000 rpm, 30 minutes, 4 °C) and purified using Ni2+-affinity chromatography (Ni-NTA superflow cartridges, Qiagen). Typically two 5 mL columns were used for a 2 L culture in which the lysate was loaded on the column washed with 10 CV wash buffer (50 mM Hepes ...
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bioRxiv - Biochemistry 2021Quote: ... the cell lysate was cleared by centrifugation (20000 rpm, 30 minutes, 4 °C) and purified using Ni2+-affinity chromatography (Ni-NTA superflow cartridges, Qiagen). Typically two 5 mL columns (flow 5 mL/min ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant obtained after high speed centrifugation (20 000 x g for 30 min at 4 °C) was loaded on a gravity nickel-nitrilotriacetic acid (Ni-NTA) metal affinity column (Qiagen), washed with 10 column volumes (CV ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells attached to the collagen matrix were fixed with 4% PFA for 10 minutes at room temperature for IF analysis or lysed with RLT buffer (Qiagen) for RNA isolation and qRT-PCR analysis as described above.
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bioRxiv - Microbiology 2020Quote: ... Media was refreshed 4 and 8dpi and surviving cells were collected 12dpi for genomic DNA extraction using QIAamp DNA Blood Maxi Kit (Qiagen). gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA (2000 ng) was transcribed into cDNA in a volume of 20 μl using 4 U Omniscript reverse transcriptase (Qiagen). To determine the relative abundance of the mRNAs of interest ...
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bioRxiv - Genomics 2021Quote: ... cfDNA extraction was performed using the QIAamp Circulating Nucleic Acid Kit using the 4-mL plasma protocol (Qiagen, product #55114). Prior to DNA elution ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from pools of 6-10 dissected hearts of 4-OHT-treated cmcl2/RFP and cmlc2/GFP-HRAS juvenile fish that were homogenized in Qiazol (Qiagen) with a TissueLyser (Qiagen) ...
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Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projectionsbioRxiv - Developmental Biology 2022Quote: ... Sorted ßGal+ or GFP+ cells were collected in 1.5ml DNA LoBind® tubes placed at 4°C containing 400µl RLT buffer (Qiagen, 79216) with 1% β-Mercaptoethanol (Millipore Sigma ...
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bioRxiv - Molecular Biology 2022Quote: His-tagged G5845–1508 (His-G5845–1508) (4 pmol) were coupled to Ni-agarose resin (25 μl of beads suspension) (Qiagen) during 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... total RNA was isolated from laser-microdissected control and mutant samples (n = 4) using the RNeasy Micro Plus Kit (Qiagen). The isolated RNA was checked for purity and integrity using Nanodrop spectrophotometer and TapeStation (Agilent) ...
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bioRxiv - Microbiology 2021Quote: ... and processed for sampling within 4 hours of collection using the MO BIO PowerSoil DNA Isolation Kit (QIAGEN; Germantown, MD). DNA in the samples was amplified via PCR with primers targeted to the V1-V3 region of the 16S rRNA ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA from sorted CD45-EpCam+UEA1+ and CD45-EpCam+UEA1-thymic stromal cells from 4-week-old mice were isolated using the RNEasy kit (Qiagen). cDNA was synthesized from the total RNA and used as a template for real-time relative quantitation for Foxn1 using commercially available probes and reagents (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... and the lysate was mixed gently with 4 ml (50% slurry) of nickel-nitrilotriacetic acid (Ni-NTA)-agarose resin (Qiagen) at 4°C for 1 h ...
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bioRxiv - Cancer Biology 2020Quote: ... we utilized 4 mL of plasma and cfDNA was isolated using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany). The concentration of cfDNA was determined using the Qubit dsDNA High Sensitivity Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: RNA from isolated fresh B cells and B cells activated with LPS/IL-4 was extracted using a RNeasy kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 20,000 rpm for 1 hour at 4°C and the soluble fraction was affinity purified by gravity column with Ni-NTA affinity resin (QIAGEN). The protein was eluted by 50 mM Tris pH 8 ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris was removed by centrifugation at 20000 g for 40 min at 4 °C and the lysate incubated with Ni-NTA Agarose Beads (Qiagen). After washing ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was clarified by centrifugation at 10,000 × g for 30 mins at 4 °C and the supernatant was applied to an Ni-NTA affinity column according to the manufacturer’s instructions (Qiagen, Germany). Bound proteins were washed twice with wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Genetics 2019Quote: RNA was extracted from skin fibroblasts of individual II.4 and a healthy unrelated control using Rneasy RNA kit (Qiagen) then we performed reverse transcription using the iScriptTM cDNA Synthesis Kit (BioRad ...
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bioRxiv - Biochemistry 2019Quote: ... The lysate was centrifuged for 10 min at 16,200xg 4°C and 600 μL of the supernatant was added to a 50 μL slurry of Ni-NTA Agarose (Qiagen) that had been washed three times with 250 μL A3B-CTD lysis buffer without 2-mercaptoethanol and once with 250 μL of A3B-CTD lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... worms were homogenized in PBS (50 μl/adult worm pair or 50 μl/1000 schistosomula) at 4°C using a TissueLyser II (Qiagen), homogenates were incubated overnight with mixing at 4°C and the supernatants collected by centrifugation at 15,000 g for 1 h at 4°C ...
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bioRxiv - Physiology 2021Quote: ... was homogenized using the Fisherbrand Bead Mill 4 and total RNA was extracted from homogenates using the RNeasy Tissue Mini Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µL of the mixture was then incubated for 45-60 minutes at room temperature or 4°C with 10 µL NiNTA resin (Qiagen) prewashed with His-SUMO Lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from 700,000 to 1.25 million RGCs from 4 independent rat litters per developmental stage was isolated using the Qiagen RNAeasy kit from Qiagen, which included a 15 minutes On-column DNAse step ...
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bioRxiv - Genomics 2021Quote: Strains were grown overnight in 50 ml YPD broth and genomic DNA was extracted from approximately 4×109 cells using a QIAGEN Genomic-tip 100/G kit (10223, QIAGEN) with minor modifications ...
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bioRxiv - Immunology 2021Quote: DNA was extracted from feces pellets using the Qiagen MagAttract PowerMicrobiome DNA/RNA EP kit (QIAGEN, Cat#27500-4-EP). The V4 region of the 16S rRNA-encoding gene was amplified from extracted DNA using the barcoded dual-index primers developed by Kozich et al ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from a pool of 20 mechanically homogenized embryos at 96 hpf for each sample (n=4) using TRItidy G (AppliChem) and 500 ng of cDNA was synthesized using QuantiTect Reverse Transcription Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... Lysates were clarified by centrifugation at 12,000xg at 4°C and mixed with 200 µL of a slurry of Ni2+-NTA agarose (Qiagen #30210) that had been equilibrated in lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... 45 min at 4 °C) and supernatant containing His-tagged proteins were purified by nickel-nitrilotriacetic acid (Ni-NTA) purification (Qiagen). Protein was eluted in a final elution buffer of 20 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: We euthanized groups of 20 to 30 zebrafish larvae by immersion in ice-cold water (below 4°C) and immediately performed RNA extraction using the RNeasy Mini Kit (Qiagen) and reverse transcription using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Passage 0 and 4 organoids were dissociated to single cell suspensions and DNA extracted using the QIAamp DNA Micro kit (Qiagen). The extracted genomic DNA was sheared and the fragments were end repaired ...
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bioRxiv - Cell Biology 2022Quote: ... RNA of LDOs in EM on day 4 after passage was extracted using the RNeasy Mini kit (QIAGEN, Hilden, Germany). After cDNA synthesis using the QuantiTect Rev ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were separated on a 4% agarose gel at 110V for 40 min and proper-sized products extracted using QIAquick gel extraction kit (Qiagen). Following denaturation in undyed loading buffer at 95°C for 3 min ...
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bioRxiv - Neuroscience 2023Quote: RNA was isolated from hippocampal tissues of 4- and 9-month-old WT and Tau mice using RNeasy Mini kit from Qiagen with DNase digestion ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted from the supernatant (12,000 x g for 10 min at 4°C) of centrifuged tissue homogenate using an RNeasy Mini Kit (Qiagen, USA). The extracted total RNA was used for single-strand cDNA synthesis ...
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bioRxiv - Genomics 2024Quote: ... Fosmid clones from the WIBR-1 library were purchased from the BACPAC Resources Center (for coordinates and names, see Supplementary Table 4) and isolated using Large-Construct Kit (Qiagen).
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bioRxiv - Biochemistry 2024Quote: ... Lysed cells were sedimented by centrifugation at 20,000 RCF at 4 °C for 30 min and the lysate was subsequently loaded onto pre-equilibrated Ni-NTA agarose resin (Qiagen) at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Cytosolic fractions were prepared by centrifugation at 10,000 × g for 30 min at 4°C and DNA was isolated from them using the DNeasy Blood & Tissue kit (Qiagen). The copy number of mitochondrial DNA encoding 16S RNA (RNR2 ...