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Citations for Qiagen :
2251 - 2300 of 3100 citations for 6 methyl 3 propan 2 ylcyclohex 2 en 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... HIV-1 RNA in plasma was extracted using the QIAamp Viral RNA Mini Kit (Qiagen). To amplify the 3’ half viral genome ...
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bioRxiv - Microbiology 2024Quote: ... All samples were subjected to a 1-hour DNAse treatment (Qiagen RNase-free DNase kit), and final elution volume was 20 μl ...
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bioRxiv - Immunology 2024Quote: ... Illumina compatible libraries were generated using the Qiagen Q1ASeq 1-step amplicon kit (Qiagen, #180419), and sequencing was performed on the Illumina MiSeq platform using 2×250 paired end reads with a goal of generating 5,000 – 10,000 reads per sample ...
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bioRxiv - Biochemistry 2024Quote: ... 30 min) and supernatant was loaded onto a 1 ml Ni-NTA Superflow column (Qiagen). Proteins were eluted by an increasing imidazole gradient according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 1 × 106 cells using the RNeasy Plus Mini Kit (Qiagen), and cDNA was synthesized with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Afterwards the pellet was resuspended in 1 ml of 100 % RNAlater/ RNAprotect Tissue Reagent (Qiagen) and kept on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... Complementary DNA was synthesized from 1 μg of RNA using the QuantitTect RT kit (Qiagen). Quantitative PCRs (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... RNA extractions substituted lysis buffer containing proteinase K solution in PKD buffer (1:16) (Qiagen) for the Mag-Bind beads used in the manufacturer protocol.
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bioRxiv - Genomics 2023Quote: ... Button valves were opened and biotinylated anti-pentaHis antibody (Qiagen, 1:4 dilution in HEPES) was flowed for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and total RNA (1 µg) was reverse transcribed using QuantiTect Reverse Transcription Kit (Qiagen, 205313) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... total genomic DNA was extracted from 1×106 parasites using DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... the precipitates were subjected to Western blot analysis with anti-penta-His (1:2000, Qiagen) and anti-FLAG M2 (1:4000 ...
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bioRxiv - Cancer Biology 2023Quote: ... After chloroform: isoamyl alcohol (24:1) extraction followed by purification (Qiagen RNeasy Mini Kit, 74104), the biotinylated RNA was resuspended in water and biotinylated-RNA was isolated using Dynabeads MyOne Streptavidin C1 magnetic beads (Invitrogen) ...
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bioRxiv - Systems Biology 2023Quote: Approximately 5 mg of frozen prefrontal cortex was homogenized in 1 ml RLT buffer (Qiagen) using a BeadBeater (BioSpec ...
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bioRxiv - Bioengineering 2023Quote: ... and purified from 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen, Germantown, MD). A double-stranded insert was digested at the same restriction sites ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using a 10:1 ratio of binding buffer (a modified version of Qiagen’s PB buffer) to digestive mixture ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 hour and purified by agarose gel extraction (MinElute Gel Extraction Kit, Qiagen #28606). Plasmid constructs were created by Gibson cloning with NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621L ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... Amplified products were resolved on a 1% agarose gel and purified by gel extraction (QIAGEN). Electrocompetent Enterobacter sp ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellets were resuspended in 1 ml Qiagen RNA protect reagent (Qiagen, Venlo, Netherlands) and incubated for 24 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... following the manufacturer’s instructions and treated twice with DNase I (1 U/μg RNA, Qiagen). The RNA concentration was quantified using the Nanodrop2000 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from 1×106 MEL cells with the RNeasy Plus kit (Qiagen, 74134). RNA was reverse-transcribed using the Superscript III First-Strand Synthesis System (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Four-cell and morula stage embryos were collected into 1 × TCL buffer (Qiagen, Cat# 1070498) containing 1% 2-mercaptoethanol at 46 and 72 h post-fertilization ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 500 µL AP1 lysis buffer and 10 µL proteinase K (10 mg mL-1; Qiagen) were added to each tube ...
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were extracted from 1 million cells using the RNeasy Mini Kit (Qiagen, 74136) and treated with TURBO DNA-freeTM Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from ∼1 million cells using the RNeasy Mini Kit (Qiagen, #74104) with QIAshdredder Mini Spin Columns (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... and the remaining lysates were incubated with 1 mL of Ni-NTA agarose (Qiagen, 1018244) on nutator (Daeiltech ...
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bioRxiv - Immunology 2024Quote: Up to 1 x 105 cells were FACS sorted directly into RLT lysis buffer (Qiagen) supplemented with 14mM β-mercaptoethanol ...
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bioRxiv - Microbiology 2024Quote: ... About 1 A600 unit of culture was harvested and RNA was extracted using RNeasy (Qiagen). cDNA was synthesized after ezDNase treatment using the SuperScript VILO cDNA kit (Thermo Fisher) ...
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bioRxiv - Plant Biology 2024Quote: ... 1 microgram of total RNA was reverse transcribed using the QuantiTect Reverse Transcription kit (Qiagen). Real-time RT-PCR was performed on 1:10 diluted cDNA using the SensiFAST SYBR No-ROX Kit (Bioline) ...
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bioRxiv - Developmental Biology 2021Quote: ... for 1 hour at room temperature followed by isolation with a minElute RNA Cleanup Kit (Qiagen). 5 μg DNase-treated RNA was used as input for ribosomal depletion using the Epicentre (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of RNA was reverse-transcribed to cDNA using the Quantitect Reverse Transcription Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Snap frozen tissue samples or cells were homogenized in 0.5-1 ml Qiazol (Qiagen, Hilden, Germany) using a Retsch Homogenizer (Retsch GmbH ...
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bioRxiv - Molecular Biology 2021Quote: RNA was harvested from cell pellets of 1 million cells using the RNeasy Mini kit (Qiagen). DNA was digested using the TURBO DNA-free Kit (Ambion ...
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bioRxiv - Genetics 2020Quote: ... Conditions for a 25 μl PCR reaction using the 1 × Taq PCR Master Mix kit (Qiagen) were ...
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bioRxiv - Genomics 2019Quote: ... All wells were then pooled and diluted 5:1 (∼24 mL) in Buffer PB (Qiagen #19066) with 1/20th volume of 3 M NaOAc pH 5.2 (∼1.2 mL) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 μg of RNA was reverse-transcribed into cDNA using the RT Omniscript cDNA kit (Qiagen), and 20 ng of cDNA template was amplified on a LightCycler-480 (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were subsequently homogenized at RT for 1 minute at 30Hz (Tissue Lyser II, Qiagen). 20ul of each homogenate was removed for protein concentration measurements (Pierce BCA Protein Assay Kit ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 RNA was extracted from plasma samples using QIAamp Viral RNA Mini Kit (Qiagen, #52904). Followed by first-strand cDNA synthesis using Superscript III reverse transcriptase (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... Richard Vierstra24,54, and phytochrome 1 from Agrobacterium fabrum strain C58 (AgP1, gene Atu1990) in pQE12 (Qiagen) was a kind gift from Prof ...
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bioRxiv - Biochemistry 2020Quote: ... The cytosolic supernatant was incubated and stirred with 1 ml Ni-NTA agarose (Qiagen, Hilden, Germany) at 4 °C for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... and Halt Protease inhibitor (Thermo-Scientific) (1:100) for 1min at 30Hz using the TissuelyserLT (Qiagen). Homogenates were transferred to chilled Eppendorf tubes (−20°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using mouse anti-His antibody from Qiagen (Cat No./ID: 34660 1:500). Anti-mouse HRP was used for colorimetric development (Cat No./ID-A8924 1:1000) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1 μg of total RNA was reverse transcribed using the Quantitect Reverse Transcription Kit (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM PMSF and the resulting supernatant was incubated with nickel-nitrilotriacetic acid agarose (QIAGEN) overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA (1 µg) was isolated from liver tissues using QIAzol lysis reagent (Qiagen, Hilden, Germany) and purified with RNeasy mini kit (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was extracted using the RNeasy Plant Mini Kit with in-column Dnase 1 digestion (Qiagen). 200 ng of total RNA were reverse transcribed using 500 ng of oligo(dT)15 and the ImProm-II Reverse Transcription System following the manufacturer’s instructions (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... the purification column was treated with 40μl of DNAse (diluted 1/8 in RDD Buffer) (Qiagen), incubated 15min and centrifuged at 8000×G for 15sec ...
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Cancer causes dysfunctional insulin signaling and glucose transport in a muscle-type specific mannerbioRxiv - Physiology 2021Quote: ... Muscles were homogenized 1 min at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in 300 μl ice-cold homogenization buffer ...
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bioRxiv - Microbiology 2021Quote: Total RNA was prepared from 5×106-1×107 cells using the RNeasy MIDI Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA synthesis was performed from 1 μg of total RNA using the Omniscript RT Kit (Qiagen). All the samples were analyzed in triplicate using GoTaq qPCR Master Mix (Promega ...