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Citations for Qiagen :
2201 - 2250 of 2355 citations for Propane 1 2 Diyl Diacetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 30µL of DNase and RDD buffer (1:7 ratio) containing solution from the RNase-Free DNase Set (Qiagen, Catalog No. 79254) was incubated on the columns at room temperature for 15 minutes before a second and third round of RNA was performed as well as a final spin to ensure the column was dry ...
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bioRxiv - Cancer Biology 2022Quote: ... after which we select the top 250 genes for which the correlation coefficient was positive and the top 250 genes for which the correlation coefficient was negative. The final step of the pipeline (Fig. 1 (4)) analyzes these genes by using Ingenuity Pathway Analysis (IPA, QIAGEN, [27]) to interpret the canonical pathways.
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bioRxiv - Cell Biology 2022Quote: ... were digested with DpnI for 1 hour at 37°C and subsequently purified with the QIAquick Gel Extraction Kit (Qiagen #28704). They were ligated with homemade Gibson Master Mix (Table S1 ...
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bioRxiv - Biophysics 2024Quote: ... Cleavage products were separated on a 1% agarose gel and the linearised plasmid was purified with a QIAquick Gel Extraction Kit (Qiagen, #28706). Complementary synthetic DNA oligos coding for miR-34a target sites (Table S1) ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were initially preserved with 1 mL of activated sludge (i.e., suspended phase) in 9 mL of LifeGuard Soil Preservation (Qiagen, Hilden Germany). This solution was thawed and centrifuged (16,000 g for 1-minute ...
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bioRxiv - Microbiology 2024Quote: ... samples were thawed and homogenized at a frequency of 26/s for 1 minute in a TissueLyser II (Qiagen, Germantown, MD) and centrifuged at 16,500xg for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from the cortical brain and cochlea samples from 1-month-old mice using RNA extraction kit and Qiazol Reagent (RNeasy mini kit; Qiagen, Germany), and DNase treatment was performed (RNase-free ...
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bioRxiv - Immunology 2023Quote: DNA was extracted from 1×10^6 CD4 Th cells/ replicate using the Qiagen DNA mini kit according to manufacturer’s instructions (Qiagen Hilden, Germany). DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Evolutionary Biology 2022Quote: ... The concentration of the resulting His-tagged ß-CATENIN-1 fragments was quantified via quantitative Western blotting with a Penta-His antibody (Qiagen) and a recombinant His-tagged protein as external standard.
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bioRxiv - Cell Biology 2023Quote: ... total RNA was isolated from C2C12 myoblasts transfected with GFP-empty vector or GFP-Δ(1-240)JPh1 using the RNeasy-plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in ice-cold PBS and resuspended in 350μl of Buffer RLT Plus supplemented with 1% (v/v) β-mercaptoethanol from the RNeasy Plus Mini Kit (Qiagen, 74134). RNA was extracted using the same kit according to manufacturer’s instructions and quality was assessed using the Agilent 2200 TapeStation System (Agilent ...
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bioRxiv - Biochemistry 2022Quote: ... Memorial Sloan Kettering Cancer Center) at a dilution of 1:5,000) and His (mouse anti-penta-His antibody (Qiagen, cat. #34660) at a dilution of 1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... a small amount of tissue (>25 mg) was added to 1 mL of TRIzol with a single 5mm stainless steel bead (Qiagen, Germany) and homogenised using a TissueLyser II (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: ... samples were suspended in RNeasy kit Lysis Buffer and β-mercaptoethanol (1:100 v/v) and homogenized using QIAshredder columns (Qiagen). Samples loaded on spin columns were treated with DNase (Qiagen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following an incubation at 55°C for 1 hr in 450 µl of lysis buffer with proteinase K (QIAGEN, Hilden, Germany). Each colony was genotyped using twelve microsatellite loci69 ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting amplicon was cut from a 1 % agarose gel and purified using the QIAGEN PCR purification kit (QIAGEN, Hilden, Germany). Afterwards ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... The digested vectors were run on 1% agarose gel and the target band was cut and harvested using QIAquick Gel Extraction Kit (QIAGEN 12362). The gel extraction products were further purified with QIAGEN MinElute PCR purification kit (QIAGEN 28004) ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by additional centrifugation at 14,000 xg for 30 minutes and supernatants were incubated 1 hour at 4 °C with 5 mL of His-Pur™ Ni-NTA resin (Qiagen) previously washed 3 times with 25 mL of Lysis Buffer ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated from BCBL-1-RTA at the indicated times following reactivation using the Dneasy blood and tissue kit following manufacturer guidelines (Qiagen 69581). 20ng of total DNA was used for qPCR using primers for the KSHV gene ORF57 ...
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bioRxiv - Neuroscience 2024Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For coimmunoprecipitation (coIP ...
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bioRxiv - Bioengineering 2024Quote: ... IVD cells that were attached to the well were washed with 1% dPBS and lysed using RLT buffer (Qiagen, Hilden, Germany) and 1% mercaptoethanol (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... additional DNA from 1-5 mL of plasma was extracted using the QIAamp® Circulating Nucleic Acid Kit (Qiagen, catalogue # 55114). Illumina sequencing libraries were constructed using SRSLY® PicoPlus DNA NGS Library Preparation Kit (ClaretBio – Cat ...
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bioRxiv - Cell Biology 2024Quote: ... The digest was run on an 1% Agarose gel (containing ethidium bromide) and the pLV6 backbone was extracted with the QIAquick Gel extraction kit (Qiagen 28704) according to the manufacturer’s manual.
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bioRxiv - Cell Biology 2024Quote: Cell pellets or lung lobes from individual mice were snap frozen in 350µl of 1:100 β-mercaptoethanol in the RLT Lysis Buffer from the RNAeasy mini plus Kit (Qiagen, 74134). Cell samples were thawed and vortexed while lung samples were homogenized for total RNA extraction intended for qPCR or bulk RNA-seq ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg of RNA was used as input for cDNA synthesis using the RNA QuantiTect transcription kit (Qiagen, Cat # 205311). Approximately 100 ng of cDNA was used as a template for each qRT-PCR reaction using the PowerTrack SYBR Green supermix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... NEM was subsequently aspirated and the tissue homogenized for 1 min at 30 Hz using a TissueLyser II bead mill (QIAGEN, USA) in ice-cold homogenization buffer [10% glycerol ...
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bioRxiv - Microbiology 2024Quote: The total RNA from the treatment group exposed to DDR (50 μg mL-1) and the blank control cultured in ABTGC medium was extracted using the RNeasy mini kit (Qiagen, Germany), followed by DNase treatment (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... The following DNA extraction methods were tested: DNA protocol 1 (DNA_P1): QIAsymphony® PowerFecal® Pro DNA Kit (cat. no. 938036, Qiagen) including six rounds of homogenization using the FastPrep-24™ Classic (MP Biomedicals ...
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bioRxiv - Microbiology 2024Quote: Whole blood from two sooty mangabeys was collected and PBMCs were purified via Ficoll density gradient centrifugation and RNA from 1 million cells was extracted according to the manufacturer’s instructions (RNeasy Mini Kit, Qiagen cat# 74104).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Media and Tryp-LE was aspirated and pelleted cells were lysed with 600μL of 1% b-mercaptoethanol (Thermo, Cat. #21985023) diluted in Buffer RLT solution (Qiagen, Cat. #79216), according to manufacturer recommendations ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was extracted from ∼1 × 107 cells using a modified version of the Gentra Puregene Cell Kit cell extraction protocol (Qiagen, 158745). Integrity of genomic DNA and absence of contaminating RNA was confirmed via 1% agarose 1X TAE gel electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were isolated from 1 mL of individual cultures using a plasmid purification kit (QIAprep Spin Miniprep Kit; Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... chemically competent S17-1 λpir cells were transformed as above with pRDH10::pgtE-FLAG isolated via QIAprep Spin Miniprep kit (Qiagen #27106) from CC118 λpir pRDH10::pgtE-FLAG ...
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bioRxiv - Immunology 2024Quote: ... Successful amplification was confirmed with an 1% agarose gel and amplified HDR template was purified with a MinElute PCR Purification Kit (Qiagen, 28004) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... and microglia (treated for 6 hours) treated with or without 1 µM CDDO-Me with a RNeasy Mini Kit (Cat. number: 74106; QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Cultured primary cells were rinsed once with ice-cold PBS and collected in freshly prepared extraction buffer consisting of 1% β- mercaptothanol in RLT (Qiagen). Saline-perfused ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR was performed with 1:10 diluted cDNA using the SYBR green detection system (Thermo Fischer Scientific, F415L) and Rotorgene Q (Qiagen, 9001560) using the 18S rRNA gene as the internal control ...
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bioRxiv - Cell Biology 2024Quote: RNA at the defined stages of differentiation (day 0, 1, 5, 8, 11, 14, 17, 27, 35, 50) was extracted using RNeasy Plus mini kit (Qiagen, 74136) according to the manufacturer’s guidelines ...
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bioRxiv - Genetics 2024Quote: ... PCR amplicons were run on a 1% agarose gel and a band of the expected size was extracted using the QIAquick Gel Extraction Kit (Qiagen 28704). The resulting amplicon was cloned into competent bacteria using the TOPO-TA Cloning Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... PCR amplicons were run on a 1% agarose gel and a band of the expected size was extracted using QIAquick Gel Extraction Kit (Qiagen, 28706). Purified amplicon DNA (0.01 pg ...
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bioRxiv - Genetics 2024Quote: ... Target sequences were amplified using self-designed primers (Suppl. Table 1) and pyrosequencing was performed using the PyroMark Q24 technologies and corresponding Gold Kits (Qiagen, Germany). Non-template controls were included in all steps to rule out contaminations ...
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bioRxiv - Evolutionary Biology 2024Quote: ... They were placed individually into 2 ml microcentrifuge tubes with 1 ml of distilled water and a stainless-steel bead (Ø 5 mm, and homogenised with a Qiagen TissueLyser LT at a frequency of 30 Hz for one minute ...
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bioRxiv - Genomics 2021Quote: ... was run in a 1% agarose gel and the desired band (1.8 kb) was purified using QIAquick gel extraction kit (Qiagen, Cat. No. 28704) and used as a template for the transcription reaction ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from the supernatant of cells infected with Candid #1 (P0 and P11) using the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) into the LB media and purified by Ni-NTA affinity chromatography (Qiagen, USA). The full length of ASSCP2 protein (ASSCP2-R ...
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bioRxiv - Developmental Biology 2020Quote: ... Japan) and 1 μL of the cDNA synthesis reaction mixture was used with the Platinum SYBR Green kit from Qiagen (Hilden, Germany). q-PCR was performed using a Rotor-Gene Q Real-time PCR machine (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: PCR with forward and reverse primers (see Table 1) was performed on mouse hypothalamic cDNA (isolated with miRNeasy Mini Kit, Qiagen, Hilden, Germany). PCR products were ligated into pGEMT.easy (Promega ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were resolved on a 1% w/v agarose gel and purified using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and damaged OA cartilage with a Mankin score of 4 or higher (n=1, female, 80 years old) using MiRNeasy Kit (Qiagen, Germantown, USA). RNA was quantified using the Nanodrop 1000 Spectrophotometer (Thermo Fisher ...