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Citations for Qiagen :
2201 - 2250 of 10000+ citations for Cow Four And A Half LIM Domains Protein 2 FHL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The clarified supernatant containing the target protein was incubated with pre-equilibrated Ni-NTA resin (Qiagen) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were transferred to nitrocellulose membranes before being revealed with anti-Histidine monoclonal antibodies (Qiagen). Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega ...
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bioRxiv - Molecular Biology 2021Quote: Proteins were purified from Sf21 insect cells by affinity purification with Ni-NTA agarose (Qiagen, 30230) and ...
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bioRxiv - Immunology 2021Quote: ... Insoluble material was removed by centrifugation and 6xHis-tagged protein was purified using Ni-NTA (Qiagen) and gravity chromatography ...
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bioRxiv - Systems Biology 2021Quote: ... PBMCs were thawed on ice and then lysed to protein fraction using Allprep Spin Columns (Qiagen) according to the manufacturer’s instructions with the QIAshredder lysis option ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing hexahistidine-tagged proteins was passed over a column of Ni2+-NTA matrix (Qiagen) at 0.5 mL matrix / 1 L of culture ...
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bioRxiv - Plant Biology 2021Quote: ... HIS-tagged proteins were purified by metal-affinity chromatography using Ni-NTA resins (Qiagen, Hilden, Germany). Protein concentration was estimated by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Denaturing purification of the protein with urea was carried out using the Ni-NTA Agarose (Qiagen). Immunisation of rabbits was carried out at Eurogentec ...
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bioRxiv - Microbiology 2021Quote: ... The LuxT-6xHis protein was purified from the clarified supernatant by Ni-NTA Superflow resin (Qiagen). Following washes with lysis buffer containing 20 mM Imidazole ...
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bioRxiv - Cancer Biology 2021Quote: ... washed again and proteins were visualized by SuperSignal™ West Dura Extended Duration Substrate (Qiagen #34076)
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bioRxiv - Plant Biology 2021Quote: ... Protein extract (1 mg) was incubated with His6-RabD2c (1 μg) and Ni-NTA Agarose (QIAGEN) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tagged protein was purified by affinity chromatography on Ni-NTA agarose beads (Qiagen 30230) and cleaved on beads with TEV protease o/n at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The protein sequences were aligned by MUSCLE [76] and visualized by CLC Genomic Workbench 8.5.1 (Qiagen). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... Protein expression was performed using standard protocols and purification was performed using Ni-NTA Agarose (Qiagen). Recombinant RelMtb protein (87 kDa ...
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bioRxiv - Plant Biology 2019Quote: ... The protein was purified under denaturing conditions on Ni2+-nitrilotriacetic acid matrix (Ni-NTA Agarose, Qiagen) and used to raise polyclonal antibody in rabbits (Eurogentec) ...
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bioRxiv - Synthetic Biology 2021Quote: 6His-tagged Mdb1 recombinant protein was expressed in BL21 and purified using Ni-NTA beads (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... 20 mM imidazole] and BiP proteins were bound to 80 µl Ni-NTA agarose beads (Qiagen) for 30 min at 25 °C in the presence of 0.01% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... The His-tagged target protein was purified by Ni-NTA gravity-flow chromatography (Qiagen, Hilden, Germany). The eluted protein was subjected to MonoQ 10/100 GL (GE Healthcare Lifesciences ...
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bioRxiv - Plant Biology 2022Quote: Protein sequence alignments and phylogenetic analyses were obtained with the CLC Main Workbench v6 software (Qiagen). Phylogenetic tree was used from matrix pairwise data and using UPGMA algorithm ...
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bioRxiv - Systems Biology 2022Quote: ... RNA was isolated according to the manufacturer’s instructions (AllPrep DNA/RNA/Protein Mini, Qiagen, Austin, Texas). RNA quality was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... The fusion protein in the lysate was affinity-purified by Ni-NTA agarose beads (Qiagen, 30210) capturing HIS tag or by glutathione sepharose 4B beads (Amersham ...
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bioRxiv - Immunology 2024Quote: ANKRD55 interactome proteins were analyzed with the use of Ingenuity Pathway Analysis (IPA) software from QIAGEN (https://digitalinsights.qiagen.com/IPA) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins modified with 6×-His-Ub was purified using Ni-NTA Sepharose (142350243, Qiagen, Alameda, CA) on a rotating platform for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins identified from proteomics were mapped to existing biological networks by Ingenuity Pathway Analysis (QIAGEN, CA)
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bioRxiv - Cell Biology 2022Quote: ... Recombinant proteins were purified on Ni-NTA columns (1 ml Ni-NTA Agarose; Qiagen Cat # 30210), washed with 20 ml hexokinase buffer (20 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was then purified on Ni-NTA affinity chromatography with Ni-NTA agarose resin (Qiagen) following the protocol described in Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant containing each target protein was loaded onto a Ni-NTA (nitrilotriacetic acid) column (Qiagen) which was pre-equilibrated with the corresponding Lysis Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 500 mM KCl and fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen) and HiTrap Q HP (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... 500 mM KCl and the fusion proteins purified from clarified lysate through consecutive Ni-NTA (Qiagen) and HiTrap Q HP (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... and the supernatant was used for bacterial protein purification using Ni-NTA affinity resin (Qiagen: 30210).
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bioRxiv - Immunology 2024Quote: ... Proteins and complexes were purified from the cell supernatants using Nickel-NTA affinity resin (Qiagen: 30210), followed by Superose 6 (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... 1% v/v sodium dodecyl-sulfate pH 8.0) for protein extraction or in RLT buffer (Qiagen) for RNA extraction ...
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bioRxiv - Biochemistry 2024Quote: ... WT and mutant proteins were initially purified using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAgen). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... Total genomic DNA extraction was performed using a commercial genomic DNA kit (DNeasy Blood & Tissue Kit, Qiagen). Photoproducts were quantified using a pre-optimized procedure (Thierry Douki ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated using the RNeasy Mini plus Kit or the RNeasy Micro plus Kit (Qiagen). After determining RNA concentrations by spectrophotometry ...
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bioRxiv - Microbiology 2021Quote: ... blank DNA extraction kits (N=14)) controls using the DNeasy PowerLyzer Powersoil kit (Qiagen, Germamtown, MD, USA), with minor modifications to the manufacturer’s protocols as previously described [109 ...
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bioRxiv - Physiology 2020Quote: ... DNA was isolated using Qiagen MagAttract PowerMicrobiome kit DNA/RNA kit (Qiagen, catalog no. 27500-4-EP) on the EpMotion 5075 (Eppendorf ...
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bioRxiv - Microbiology 2020Quote: ... RNA from Trizol samples was purified using a commercial kit (RNeasy® Mini Kit, Qiagen no. 74104) with additional on-column DNAse treatment (Qiagen no ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated using an RNeasy Mini Kit with the addition of RNase-free DNase kit (Qiagen) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cellular RNA was extracted using the QIAshredder kit and the RNeasy Mini kit (79654 and 74106, Qiagen). The cDNA was generated using High Capacity cDNA reverse transcript kit (4368814 ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated using an AllPrep DNA/RNA Mini kit or RNeasy Plus Micro kit (Qiagen). cDNA was prepared using the SuperScript III Reverse transcription Kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The RNA extraction process was referred to the instructions of the kit (RNeasy Mini kit, QIAGEN, Germany). The extracted RNA was detected by ultra-fine ultraviolet spectrophotometer (NanoDrop ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR product was purified by either QIAquick Gel Extraction Kit or QIAquick PCR Purification Kit (Qiagen), cloned into the donor vector pDONR207 using Gateway BP Clonase (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was purified with RNeasy plus micro kit or RNeasy plus mini kit (Qiagen, Hilden, Dermany). Complementary DNA was synthesized by a SuperScript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was purified using the Xtreme DNA isolation kit (Isohelix) or DNeasy Blood & Tissue Kit (Qiagen). These kits were used to purify DNA from crude lysates or from tissue samples preserved in RNAlater solution (Sigma).
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bioRxiv - Microbiology 2021Quote: ... and viral DNA was extracted and purified using a commercial kit (DNeasy Blood and Tissue Kit, Qiagen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Amplified scFv PCR fragments were purified with Gel Extraction kit (Qiaquick Gel Extraction Kit, Cat# 28706, QIAGEN), and subjected to SfiI (Fast Digest SfiI ...
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bioRxiv - Microbiology 2022Quote: gDNA was purified from Bacteroidales using a DNeasy UltraClean Microbial Kit or DNeasy Blood & Tissue Kit (Qiagen). Up to 2 μg in 200 μL TE buffer was sheared by ultrasonication to an average of 3–5 kb with a Covaris S220 focused ultrasonicator using red miniTUBEs ...
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bioRxiv - Microbiology 2024Quote: ... an EZ1 extraction was performed using a Qiagen kit with EZ1 DNA Tissue Kit (Qiagen, Courtaboeuf, France) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested and total RNA isolated using an RNA isolation kit (RNeasy Mini kit, Qiagen, 74106). 8 µg of total RNA were treated with DNaseI and purified according to the manufacturer’s protocol (TURBO DNA-free kit ...