Labshake search
Citations for Qiagen :
2151 - 2200 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from primary mouse hepatocytes and Huh-7 cells using the RNeasy Plus Micro Kit and cDNA synthesis performed using the QuantiTect Reverse Transcription Kit according to the manufacturer’s protocol (Qiagen). Reactions were performed in triplicate in 384-well plate format ...
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CD4+ T cells regulate sickness-induced anorexia and fat wasting during a chronic parasitic infectionbioRxiv - Immunology 2022Quote: ... DNA from the blood or tissue of mice was extracted using the same kit (Qiagen blood and tissue kit) and qPCR was done using the same primers ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA removed and RNA extracted using a commercial kit (RNeasy Plus Mini Kit, Cat. 74134, Qiagen, Hilden, Germany). Sample processing for quality control and sequencing was completed by the Australian Genome Research Facility (Victoria ...
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bioRxiv - Bioengineering 2023Quote: Total RNA and cDNA were synthesized from the samples using RNeasy mini kit and QuantiTect Reverse Transcription Kit according to the manufacturer’s instructions (Qiagen). qPCR was performed in a Biorad Thermocycler with a reaction volume of 20 µl which constitutes primers (0.5 µM) ...
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bioRxiv - Cancer Biology 2023Quote: ... was isolated using Midi or Maxi kits for the validation screens gDNA was isolated using Midi kits according to the manufacturer’s protocol (Qiagen). PCR and sequencing were performed as previously described.81 ...
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bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was isolated using the SampleIN Direct PCR Kit (highQu) or the AllPrep DNA/RNA/Protein Mini Kit (Qiagen), and amplified with ALLin HS Red Taq Mastermix (highQu ...
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bioRxiv - Microbiology 2023Quote: ... Three nucleic acid extraction kits designed for DNA and RNA extraction were compared: DNeasy PowerWater kit (QIAGEN; Hilden, Germany), NucleoSpin Soil ...
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bioRxiv - Microbiology 2024Quote: ... and RNA was extracted following the manufacturers instructions (Zymo Quick-RNATM Viral Kit or QIAGEN RNeasy Mini Kit, respectively).
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bioRxiv - Microbiology 2024Quote: ... Brazil) using the QIAseq miRNA Library Kit Handbook and QIAseq miRNA 96 INDEX IL preparation kit – both from QIAGEN, following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: ... cRNAs were generated by in vitro transcription with the mMessage mMachine T7 transcription kit (Thermo) according to the manufacturer’s protocol and were purified with a RNeasy kit (Qiagen). cRNA transcripts were microinjected into surgically extracted Xenopus laevis oocytes (Ecocyte ...
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bioRxiv - Genetics 2024Quote: miR-210 expression levels in both flies and mice were quantified by quantitative real-time PCR (qRT-PCR) using the miRCURY LNA miRNA PCR assay (Qiagen, Hilden, Germany). First-strand cDNA synthesis was obtained from 10 ng of total RNA by using the miRCURY LNA RT Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: The ipsilateral hemispheres were lysed in Qiazol Lysis Reagent and total RNA was extracted using the MaXtract High Density kit with further purification using the RNeasy Mini Kit (all Qiagen). 70 ng of total RNA per sample was then hybridized with reporter and capture probes for nCounter Gene Expression code sets (Mouse Neuroinflammation codeset ...
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bioRxiv - Genetics 2021Quote: ... Cells were lysed with Precellys CKMix Tissue Homogenizing Kit (Bertin Technologies) and total RNA was extracted using Qiagen RNeasy Maxi Kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from lung homogenates according to the instructions of the RNA extraction kit manufacturer (RNeasy Plus Mini Kit; Qiagen) post phase separation using Trizol reagent ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from lung homogenates according to the instructions of the RNA extraction kit manufacturer (RNeasy Plus Mini Kit; Qiagen) post phase separation using Trizol reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA extraction was done using RNeasy Plus Mini Kit following Proteinase K digestion and elimination of DNases using RNase-free DNase kit (all reagents from Qiagen). Isolated RNA was then converted to cDNA using Bio-Rad iScript kit and RT-qPCR using Bio-Rad SYBR Sso Advanced ...
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bioRxiv - Genetics 2020Quote: ... Reverse transcription was performed using bead-bound RNA (SuperScript VILO cDNA synthesis kit) and qPCR was performed in triplicate (QuantiFast SYBR Green PCR Kit; Qiagen) on a Roche LightCycler 480 using beta-actin as reference gene ...
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bioRxiv - Microbiology 2020Quote: ... RNA extraction was performed using the QIAamp 96 DNA kit and the Qiacube HT kit and the Qiacube HT (both from Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... We extracted total genomic DNA from blood and/or tissue samples using standard nucleic acid extraction kits (QIAamp DNA Mini Kit; Qiagen) automated on a QiaCube (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: Brain samples were lysed in Qiazol Lysis Reagent and total RNA was extracted using the MaXtract High Density kit with further purification using the RNeasy Mini Kit (all Qiagen). 70 ng of total RNA per sample was then hybridized with reporter and capture probes for nCounter Gene Expression code sets (Mouse Neuroinflammation codeset ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from ∼100 mg of root tissue and DNase treated using the RNeasy plant mini kit and RNase free DNase kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... sections of small intestine from the lorikeets were deparaffinized and the DNA extracted using a commercial kit (QIAamp DNA FFPE tissue kit; Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was extracted from the same tumor region as the single-cell analyses using the Qiagen AllPrep kit and matched normal blood using DNeasy kit (Qiagen). Briefly ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed with RLT buffer and total RNA was isolated using an RNA purification kit (RNeasy Mini Kit, Qiagen). cDNAs were transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Genomics 2022Quote: DNA was extracted using either the Nanobind Tissue Big DNA kit (Circulomics, USA) or DNeasy Blood & Tissue Kit (Qiagen, Germany) by following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Samples were homogenized using a motorized pestle and total RNA was extracted and purified using a RNeasy Plant Mini Kit and RNeasy MinElute Cleanup Kit (Qiagen). RNA was sent to GENEWIZ ...
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bioRxiv - Microbiology 2022Quote: ... Cytochrome b products were excised and purified from the gel using a commercial kit (QIAquick Gel Extraction Kit, Qiagen, Germany), followed by purification of eluted DNA using AMPure XP Magnetic Beads (1X ...
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bioRxiv - Genetics 2022Quote: ... These cells were lysed with Precellys CKMix Tissue Homogenizing Kit (Bertin Technologies) and total RNA was extracted using Qiagen RNeasy Maxi Kit (Qiagen). mRNA was isolated with Oligo (dT)25 Dynabeads (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: mRNA isolation and reverse transcription reaction were performed respectively with RNeasy Mini kit and with QuantiTect Reverse Transcription kit (both from Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from Ca9-22 cells after 4 or 5 hours of culture using the RNeasy Mini prep Kit and cDNA was prepared using the RT2 First Strand Kit (Qiagen) or the qScript™ cDNA Synthesis Kit (Quanta Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: gDNA extraction for genotyping was performed on snap-frozen culture-derived cells using the DNeasy Blood & Tissue Kit and the QIAmp DNA Micro Kit from Qiagen, according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA extraction was performed using the QIAamp 96 DNA kit and the Qiacube HT kit and the Qiacube HT (both from Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... All DNA purifications were performed using a Roche High Pure PCR product purification kit or a QIAquick Gel Extraction Kit (Qiagen). Standard procedures for DNA digestion and ligation were used in conditions recommended by the enzyme manufacturer (Promega or Fermentas) ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid and genomic DNA isolations were carried out with the QIAprep Spin Miniprep Kit and the DNeasy Blood & Tissue Kit (Qiagen), respectively.
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bioRxiv - Neuroscience 2021Quote: ... Amplified cDNA was prepared from total RNA using the Ovation PicoSL WTA System V2 Kit (NuGEN) and purified with the MinElute Reaction Cleanup Kit (Qiagen). RNA quality and quantity were assessed using a 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acids were then extracted using the RNeasy PowerSoil total RNA kit in combination with the RNAeasy PowerSoil DNA Elution kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA from matched blood samples was isolated using the QIAamp DNA Blood Mini Kit or the QIAsymphony DSP DNA Mini Kit (Qiagen). Quality control was performed at the DKFZ-HIPO Sample Processing Laboratory using Quant-iT dsDNA Kit ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... Isolated genomic DNA (500 ng) of E8.5 mouse embryos (QIAamp DNA mini kit, 51304 or AllPrep DNA/RNA Micro Kit, Qiagen, 82084) and spike-in lambda DNA (1.25 ng ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was extracted from frozen tissue using the QiaAmp Mini Kit or the DNA/RNA extraction mini kit from Qiagen. DNA was quantified and quality checked with the Qubit fluorometer or the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... The DNA was extracted from FFPE tissue using the QIAamp DNA FFPE tissue kit and QIAamp DNA Micro Kit (Qiagen) for the test specimen or LCM specimen ...
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bioRxiv - Bioengineering 2021Quote: ... Two-step qPCR was performed to quantify GFP gene silencing with the following commercially available kits: RNeasy plant mini kit (QIAGEN) for total RNA extraction from leaves ...
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bioRxiv - Physiology 2020Quote: Total RNA was isolated from cells and tissues using the RNeasy Mini kit or the RNeasy Plus Mini Kit (Qiagen, Montreal ...
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription and quantitative real-time PCR (qRT-PCR) were performed with miScript II RT Kit and SYBR Green PCR Kit respectively (QIAGEN). To quantify α-Syn mRNA levels ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was transcribed from a linearized plasmid DNA template using the SP6-Scribe Standard RNA IVT Kit (CELLSCRIPT) and purified with the RNeasy Mini Kit (QIAGEN). The m7G cap structure was added to the purified RNA using the ScriptCap m7G Capping System (CELLSCRIPT) ...
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bioRxiv - Microbiology 2020Quote: ... coli isolates with QIAmp DNA mini kit or using an EZ1 Biorobot with the DNA Tissue kit (Qiagen, Hilden, Germany). The Nextera XT Kit (Illumina ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA was extracted from cell lysates using the RNase Mini Kit where genomic DNA was removed using the RNase-Free DNase Kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... Total DNA and RNA were extracted using the PowerSoil DNA isolation kit and Rneasy PowerSoil total RNA kit (Qiagen, Germany), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from the samples using either the Qiagen (Valencia, CA, USA) DNeasy Blood & Tissue kit or the AllPrep DNA/RNA Mini Kit (Qiagen) according to the manufacturer’s recommendations as outlined in Lee et al ...
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bioRxiv - Physiology 2021Quote: ... males n=11) and Ob (females n=10, males n=10) offspring using a commercially available kit (RNeasy Plus mini Kit, Qiagen) then reverse transcribed to cDNA (High Capacity cDNA Reverse Transcription kit ...