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Citations for Qiagen :
2151 - 2200 of 2525 citations for 8 Chloro 2 methylimidazol 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed by boiling (5 min 95°C) followed by bead beating (3mm beads, 30 Hz for 1 min) (TissueLyser II, Qiagen) and sonication bath (3×10 sec at 4°C ...
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bioRxiv - Genomics 2019Quote: Cells aliquoted for RNA processing were spun down and resuspended in 3.2 mL of RLT (1% ß-Mercaptoethanol) from a Qiagen RNeasy Midi kit (Qiagen). Cells were homogenized by passing the lysate through an 18-gauge needle 10 times and were stored at −80°C ...
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bioRxiv - Cell Biology 2019Quote: ... Eight to ten hundred adherent DLD-1 cells were washed with PBS and lysed directly with RLT buffer from RNAeasy Mini Kit (Qiagen). Extracted RNA was analyzed for 260/280 and RIN value on Bioanalyzer 2100 (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... and positive swabs at 3 days post infection (d.p.i.) with a Cq value < 30 (Table 1) using QIAamp viral RNA Mini kit (Qiagen, Hilden, Germany) according to the kit protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA was finally purified by reverse crosslinking at 65°C overnight followed by proteinase K (0.5 or 1 mg/ml, respectively) digestions and purifying with MinElute® PCR purification kit (Qiagen).
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bioRxiv - Genomics 2020Quote: 3 million cells from cell lines or patient samples were pelleted and resuspended in 1 mL of Cell Lysis Solution (Qiagen) mixed with 500 μg of RNase A ...
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bioRxiv - Developmental Biology 2021Quote: ... Remote biopsies were taken using an air-powered rifle affixed with a 1 mm wide by 20-25 mm deep biopsy dart and preserved in RNAlater (Qiagen). Blood samples were collected opportunistically during veterinarian visits ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand complementary DNA synthesis was performed using 1 µg of total RNA with an anchored oligo (dT) and Transcriptor Reverse Transcriptase (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... After vortexing the samples for 10 s and lysing the tissue with 4 mm glass beads for 1 min at 30 Hz in the TissueLyser II (Qiagen), 400 μL of Tris-HCl pH:7.5 were added and the samples were again mixed for 1 min in the TissueLyser ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification of p53-(1-73) from the clarified cell lysate was accomplished using a Nickel Nitrilotriacetic Acid (Ni-NTA, Qiagen) column purification and 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: DNA extraction was done from 1 ml of the different sample types using QIAamp DNA mini kit (Qiagen, Hilden, Germany), as per the vendor’s instructions with slight modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... catalysis was measured using a previously described assay (22) in which hexahistidine-tagged PARP-1 was immobilized on Ni(II)-NTA-coated plates (Qiagen) in the presence of NAD+/biotinylated-NAD+ (Trevigen) ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 integration was examined in 293T cells and J-Lat cells by isolating genomic DNA from 1 × 106 cells using the DNeasy Tissue Kit (Qiagen). Alu-LTR sequences were amplified during first round PCR from 100 ng of total genomic DNA using the following primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... several loci were amplified simultaneously from 1 μl of extracted DNA using 5 μl of the Multiplex PCR Master Mix (Qiagen) and varying amounts of the pooled primer mixes (Pool A ...
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bioRxiv - Physiology 2021Quote: ... Shredded bone marrow cells were frozen (−80°C) in RLT buffer (1% beta-mercaptoethanol) until RNA extraction using RNAeasy mini kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Immunology 2020Quote: ... mRNAs recovered from microdissected samples or vaginal biopsies (about 1 mm3) were reverse-transcribed with the QuantiTect Rev Transcription Kit (Qiagen), used according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Glass beads (3mm) and 1 mL of sterile water were added for homogenization at 30Hz for 10 minutes (TissueLyser II, Qiagen). Homogenates were diluted with PBS and inoculated onto SAB plates and incubated for 3 days at 30°C ...
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bioRxiv - Microbiology 2020Quote: ... Preparation of total RNA and Northern blotting including the determination of RNA half-lives were carried out as described previously [29] except that 1 ml time samples were taken and directly added to 250 μl RNAprotect Bacteria Reagent (Qiagen) or to 250 μl containing 5 % phenol and 95 % ethanol ...
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bioRxiv - Microbiology 2020Quote: ... The PCR fragment was then digested with Dpn I at 37 °C for 1 h and purified using the QIAquick PCR purification kit (Qiagen). To prepare the competent SW102 containing the pSMART BAC-T7-scv2-replicon ...
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bioRxiv - Microbiology 2021Quote: ... samples were weighed and homogenized in DMEM containing 10% FBS and 1% antibiotics using 5 mm stainless steel beads (Qiagen) and the TissueLyser II (Qiagen) ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmid coding for FLP recombinase and the mCherry reporter or 47bp-insertion TLR reporter plasmid were transfected into the cells at a 9:1 ratio using polyfect (QIAGEN) according to the manufacturer’s protocol (900ng mCherry reporter or 47bp-insertion TLR reporter plasmid and 100ng FLP recombinase plasmid to make 1 μg plasmid in total) ...
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Glutaric aciduria type 3 is a naturally occurring biochemical trait in inbred mice of 129 substrainsbioRxiv - Biochemistry 2020Quote: ... were homogenized in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (∼20 mg tissue per 1 mL lysis buffer) using a TissueLyser II apparatus (Qiagen) and the homogenates were centrifuged at 1,000xg ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
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bioRxiv - Bioengineering 2021Quote: ... for 1 day prior to extraction of total RNA of the treated leaves with an RNeasy plant mini kit (QIAGEN). Two-step qPCR was performed to quantify GFP gene silencing using the total RNA extracted from leaves ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was transferred to a 15 ml falcon tube and 1 ml of a 50% slurry consisting of NiNTA agarose beads (Qiagen) in resuspension buffer was added per 10 ml of lysate ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were size-selected on a 1% agarose gel and purified using the MinElute PCR Purification Kit (Qiagen). The pSTARR-seq_human vector (kindly provided by Alexander Stark ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from isolated neutrophils after the 1 and 4 hour EC challenges as well untreated controls using the miRNeasy Micro kit from Qiagen and libraries were generated using KAPA PolyA enrichment mRNA library prep ...
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bioRxiv - Biophysics 2019Quote: ... Insoluble material was pelleted for 1 hr at 35,000 rpm and supernatant was added to Ni-NTA resin (QIAGEN, 30410) in batch and rotated at 4°C for 1 hr ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were electrophoresed in a 1% agarose gel and the gel bands were isolated using QIA Quick PCR Purification Kit (Qiagen). Isolated DNA was cloned into pCR-TOPO plasmids using the TOPO TA cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Synthetic Biology 2019Quote: ... grown over night in 5 mL LB and 100 μg mL-1 ampicillin and DNA were purified using a QIAprep Spin Miniprep Kit (QIAGEN). Sequencing was performed by GATC using the primers in SI Appendix Table S4 ...
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bioRxiv - Cancer Biology 2019Quote: Genomic DNA was extracted from up to 1 mL whole blood or buffy coat using the Gentra Puregene Blood Kit (Qiagen) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... an 8 μg of the isolated RNA was further treated with 2U of TURBO DNase (Invitorgen) at 37°C for 1 h and purified with RNeasy spin columns (Qiagen). The amount of RNA was quantified with Qubit RNA BR assay kit (Invitrogen) ...
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bioRxiv - Biochemistry 2019Quote: ... The template was run on a 1% agarose gel and extracted using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). DNA template concentration was determined by a Qubit 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... This was followed by RNase A treatment for 1 h at 37°C and purification using MinElute PCR purification columns (Qiagen). cDNA amplification was performed with reporter-specific nested cDNA primers (Oligos 16 and 17 for libraries in vectors d and e ...
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bioRxiv - Genomics 2021Quote: ... To eliminate lipids supernatants were applied to a RNeasy column and centrifuged at 10,000 rpm and 4 °C for 1 min (Qiagen, 74104). The flow through was collected and protein concentrations were assessed using the Bradford assay ...
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bioRxiv - Immunology 2021Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesised from every 1 μg of total mRNA in 20 μL volume with QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacture protocol ...
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bioRxiv - Biochemistry 2021Quote: pAC5.1 vectors with CLIP-CRY and TIM-SNAP-HA were delivered into s2 cells using Effectene reagent (Cat# 301425, Qiagen) or TransIT-Insect Transfection Reagent (Cat# MIR6100 ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was purified from Achilles enthesis of 1-dpi pups or from tissue culture cells using the RNeasy Kit (Qiagen). Reverse transcription was performed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... The LNA gapmers for lnc-FLii-1 and lnc-LSAMP-3 were designed and purchased along with negative control LNA from Qiagen. For transfection ...
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bioRxiv - Cancer Biology 2021Quote: The frozen tumors were minced to small pieces and suspended in buffer P1 (QIAGEN, 1 mL Buffer/100 mg tumor) supplemented with 100 μg/mL RNase A (Promega) ...
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bioRxiv - Genomics 2022Quote: ... The indexed PCR products were cleaned using 1 Volume AMPure XP beads on magnetic stand and eluted with EB buffer (QIAGEN) followed by quantification using a Qubit dsDNA HS Assay Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... Skin samples were taken by remote biopsy dart (1 mm wide by 20-25 mm deep plug) and preserved in RNAlater (Qiagen). Blood samples were taken opportunistically during veterinarian visits and preserved in EDTA tubes ...
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bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
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bioRxiv - Genetics 2021Quote: ... In vitro transcribed mRNAs were DNAse treated using 1 μl TURBO-DNAse for 20 minutes at 37 °C and purified using RNeasy Mini Kit (Qiagen). mRNA product was quantified using NanoDrop (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... protocol 1 in the RNAprotect Bacteria Reagent Handbook (Enzymatic lysis of bacteria) was followed using the RNeasy Plus Mini Kit (Qiagen) using TE buffer (10 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... 200 mg of thawed and mixed faecal samples were added to 1 ml of InhibitEX Buffer (QIAGEN, GmbH, Hilden, Germany) followed by the beat-beating step in TissueLyser II (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and 18 centimeters below seafloor (cmbsf) from station AC of Van Keulenfjorden (Supp. Table 1) using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... ~100 mg tissue was homogenized in 1 mL Qiazol and RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were run on 1 μg of RNA per sample to afford cDNA using the QuantiTect Reverse Transcription kit (Qiagen). RT-qPCR was performed on an Applied Biosciences 7500 Real-Time PCR system following the QuantiTect SYBR Green PCR kit protocols (Qiagen ...
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bioRxiv - Immunology 2021Quote: Heat-inactivated human serum was diluted 1:10 in phosphate buffered saline (PBS) and applied to a gravity polypropylene flow column (Qiagen) containing 1 mL of Protein G-sepharose resin (Invitrogen ...