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Citations for Qiagen :
2051 - 2100 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The plasmid was transfected to 293T cells and the recombinant S protein trimers were purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography (QIAGEN, Germany), followed by size exclusion to further purify the trimers ...
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bioRxiv - Plant Biology 2020Quote: ... The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen) and analyzed by SDS-PAGE.
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bioRxiv - Physiology 2022Quote: ... Mouse Obesity (PAMM- 017ZC-12) array and Mouse Aging (PAMM-178ZC) from Qiagen (Maryland, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from donor cecal contents and day 0 conventionalized mouse feces using the Qiagen PowerFecal DNA Kit (Qiagen, Hilden, Germany; 12830) following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... eyes were enucleated and retinas were immediately dissected and total RNA was extracted from mouse retinal tissue using Qiagen RNeasy Mini Kit as per manufacturers protocol (Qiagen, Valencia, CA, USA) and reverse-transcribed using iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: RNA was extracted from mouse islets (∼300 islets per sample, pooled from ∼10 mice of the same genotype) using RNAeasy Micro Kit (Qiagen, Valencia CA, USA) as per manufactures instructions ...
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bioRxiv - Physiology 2020Quote: ... protein was extracted (Qiagen Tissue Lyser) from ∼50 mg frozen liver in tissue lysis buffer 80 ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL Protein Precipitation Solution (QIAGEN) was added ...
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bioRxiv - Cancer Biology 2023Quote: Advanced Protein Purification buffer (APP; Qiagen): the APP buffer contains zinc chloride to precipitate protein at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and The Protein Complex Suite (Qiagen). One microliter of 4.6 mg/ml protein sample suspended in crystallization buffer (25 mM Tris-HCl ...
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bioRxiv - Bioengineering 2020Quote: ... A Qiagen RT2 SYBR Green master mix with validated qPCR human primers (for HUVECs and BeWo) or mouse primers (for N27 cells) from Qiagen (Frederick) were used to determine relative magnitudes of gene-expression levels using RT-PCR ...
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bioRxiv - Bioengineering 2023Quote: Total RNA from cultured cells and mouse Achilles tendons were isolated with TRIzol reagent and RNeasy Mini Spin Column (Qiagen Sciences) and reversely transcribed into cDNAs using a SuperScript IV VILO Master Mix (Life Technologies).22,30 The relative abundances of genes of interest were determined by TaqMan PCR using primers and probes purchased from Applied Biosystems TaqMan Gene Expression Assays ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and 3.5 ml of RLT buffer with B-mercaptoethanol (10 µl for every 350 µl of RLT buffer – Qiagen) was added ...
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bioRxiv - Microbiology 2020Quote: ... RNAlater was removed from the sorted cells prior to RNA isolation and total RNA was isolated from the pelleted cells using the RNeasy mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions with some modifications ...
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bioRxiv - Microbiology 2020Quote: ... RNA from cell lysates or cell culture supernatants was extracted using the Qiagen QIAamp viral RNA mini kit (Qiagen, Valencia, CA, USA). Reverse transcription (RT ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA and DNA was extracted from the same cells from neuropathologist annotated tumour regions (>60% cancer cells) using appropriate Qiagen kits (Qiagen, Sussex, UK). Paired end strand-specific whole transcriptome libraries were prepared for 16 pairs using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Cancer Biology 2020Quote: DNA coming from CD34+ cells of the patient and Mononuclear cells of Healthy Donors was extracted by means of DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). 50ng of DNA were analysed through P056 MLPA kits (MRC Holland ...
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bioRxiv - Cell Biology 2021Quote: ... Genomic DNA was extracted from the control and the peptide-treated frozen cells using the Blood & Cell Culture DNA Midi Kit according to manufacturer’s instructions (Qiagen, ref. no. 13343). A first PCR was performed to amplify the lentiCRISPR sgRNA region using the following primers:
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA was extracted from osimertinib-resistant cells as well as matched parental cells were extracted using a DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). We reviewed all candidate alterations identified in resistant cells as well as parental cells and considered those identified only in resistant cells as candidate acquired resistance genomic alterations to osimertinib.
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bioRxiv - Immunology 2021Quote: DNA was extracted from the cultured cells or ex vivo CD4+ T cell samples using DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from purified CD4+ TN and TCM cells and from 24ST1NLESG cells using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... gDNA was isolated from 5 x 106 Colo205 cells using the Blood and Cell Culture DNA Maxi Kit (Qiagen, catalog no. 13362) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: gDNA samples from the unselected control (gDNACtrl) and 2nd (gDNAScreen) selected group cells were extracted using the Blood and Cell Culture DNA Midi Kit (#13343; QIAGEN, Germantown, MD).
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from the SBMA OL cell model and the control cell model using the RNeasy Mini Kit (Qiagen, Hilden, Germany). The quality and quantity of the RNA obtained were checked using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Influenza virus nucleic load was determined by qRT-PCR using influenza virus segment M specific primers (IAV-M; Table. 1) in 96-well plates according to the manufacturer instructions (OneStep RT-PCR, Qiagen, Toronto, Canada). Absolute quantification was performed using a standard curve based on a 10-fold serial dilution of a plasmid containing the A/Guinea Fowl/129/2015(H5N9 ...
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bioRxiv - Microbiology 2019Quote: ... A total of 25 cycles of PCRs were performed and the PCR products (∼600 bp for 5’-RACR PCR or ∼500 bp for gene-specific PCR) were gel purified (Qiagen, Valencia, CA). A degenerate primer (SAGGTGCAGCTGGTGCAGTCTGG ...
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bioRxiv - Neuroscience 2021Quote: ... target miRNAs were hybridized with 40 nM of specific DIG-labelled miRCURY probes or scramble probes diluted in hybridization buffer (Qiagen, Germantown, MD) for 1 hr at 55°C ...
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bioRxiv - Microbiology 2019Quote: ... coli cells were carried out according to established procedures [36] or to specific recommendations by the suppliers of the molecular biology reagents (Qiagen and Takara). The transformation of bacterial cells by electroporation was performed as described previously [41] ...
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bioRxiv - Synthetic Biology 2022Quote: ... A 150 bp galactose operon-specific fragment containing the region where the predicted RNA 3′-end is located was amplified using HotStarTaq™ DNA polymerase (QIAGEN, Germany) with gal-specific primer M3-F and 3RP primer (Table S3) ...
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bioRxiv - Biochemistry 2023Quote: The coding sequence of mouse Donson was amplified from XpressRef Universal Total mouse RNA (QIAGEN, 338114) by RT-PCR (Takara ...
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bioRxiv - Cell Biology 2019Quote: ... coli as an N-terminal histidine-tagged protein isolated from cell lysate after passage through Ni-NTA agarose (Qiagen, Cat. No. 30210). Bound TTRL55P was then eluted by competition with imidazole ...
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bioRxiv - Molecular Biology 2019Quote: ... the cell and molecular functions and canonical pathways of these proteins were identified using Ingenuity® Pathway Analysis (IPA®) (Qiagen, Germany).
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bioRxiv - Cell Biology 2023Quote: ... The remaining 80% of the cell suspension was lysed in 6M guanidine-HCl buffer and His6-tagged proteins were captured on Ni2+-NTA resin (Qiagen; cat. #30210). Pull-down eluates and inputs were separated on SDS-PAGE gels and analyzed by immunoblot ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell pellet was processed for total RNA isolation using an RNeasy Mini Kit (Qiagen).
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bioRxiv - Cancer Biology 2021Quote: Total mRNA was extracted from frozen cell pellets using the RNeasy Extraction Kit (Qiagen 74136). Reverse transcription and qPCR was performed using the iTaqTM Universal SYBR® Green 1-Step Kit (BioRad 1725151 ...
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bioRxiv - Neuroscience 2021Quote: Cells were harvested at DIV7 or DIV11 in buffer RLT (RNeasy RNA isolation kit, Qiagen) for total RNA extraction according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA was extracted from cells using a RNeasy Plus RNA extraction kit (Qiagen, 74136). An on-column DNase digest (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from cells using a RNeasy Plus RNA extraction kit (Qiagen, 74136) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from the specified cell lines using the RNeasy Mini Kit (Qiagen) with DNase treatment ...
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bioRxiv - Cell Biology 2020Quote: ... subsequently RNA was isolated from the cells using the RNeasy micro kit (Qiagen, Hilden, Germany) according to manufactures’ instructions ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from pelleted cells using either an RNeasy Mini kit and QIAshredder (QIAGEN) or a Direct-zol RNA Miniprep kit (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was prepared using a Qiagen Blood and Cell Culture DNA Kit (Qiagen, Venlo, Netherlands). 15-20 µg DNA was added to 124 µL of 2M CaCl2 and the total volume brought to 1 mL ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was extracted from 80 x106 cells using the QIAamp DNA Blood Maxi Kit (QIAGEN). sgRNA inserts were PCR amplified using ExTaq DNA Polymerase (Takara) ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from cells using the RNeasy kit according to the manufacturer’s instructions (Qiagen) and RNA reverse transcribed using the SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: RNA from 2×107 cells was isolated using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands), cDNA was generated by reverse transcription on CFX96 real-time system (BioRad ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from SZ95 sebocyte cells using the RNAeasy Plus universal kit (Qiagen 73404). RNA quality was assessed using Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2021Quote: ... The cells were harvested and the RNA was prepared using RNeasy Mini Kit (Qiagen, Germany) as per the manufacturers protocol ...
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bioRxiv - Genetics 2021Quote: ... and the DNA in the pellets was amplified with REPLI-g Single Cell kit (QIAGEN) for sequencing on the MiSeq or HiSeq 3000 sequencing system (Illumina).
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bioRxiv - Genetics 2021Quote: RNA from sorted microglial cells was isolated using the miRNeasy Serum/Plasma Kit (QIAGEN; 217184) and quantified on the Agilent BioAnalyzer 2100 ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was extracted from ccRCC cells using the DNeasy Blood and Tissue Kit (Qiagen). Epitect Methyl II PCR Array System (Qiagen ...