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Citations for Qiagen :
2051 - 2100 of 2849 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... 1 μg of RNA per experimental sample was processed with the QuantiTect cDNA Synthesis Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were generated using 1 ug of the total RNA via QIAseq miRNA kit (Qiagen, # 331502) with 14 cycles of amplification ...
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bioRxiv - Molecular Biology 2024Quote: Wild-type or mutant SMYD5 was purified with either 1 mL Ni-NTA Superflow Cartridges (Qiagen) or His Mag Sepharose Ni resin (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: Genomic DNA was extracted from 1 million cells using the Qiamp DNA mini kit (QIAGEN, #51304) following manufacturer instructions and DNA concentration was measured with Nanodrop 2000c (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: Small interfering RNA (siRNA) to knockdown TSP-1 expression in HUVECs was obtained from Qiagen (GS7057). AllStars siRNA was used as negative control ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were generated using 1 µg of the total RNA via QIAseq miRNA kit (Qiagen, # 331502) with 14 cycles of amplification ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from 1 × 106 cells using the RNeasy Plus Mini Kit (Qiagen; 74134), and cDNA was synthesized with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... DNA fragments were purified from a 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen). Barcoded TruSeq adapters were added to the mutant fragments by PCR (7 cycles ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was recovered using a chloroform-ethanol procedure (Supplementary protocol 1) and diluted in AE buffer (Qiagen). A pair-ended library was constructed by Eureka Genomics (Hercules ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted from 1 ml of overnight culture using a QIAamp DNA Mini Kit (Qiagen). Illumina MiSeq sequencing operated by the Functional Genomics Centre Zurich and Novogene (Cambridge ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... and also performed direct mapping against reference genome Wuhan/Hu-1/2019 (NCBI Nucleotide – NC_045512, GenBank – MN908947) using the CLC Genomics Suite v5.1.0 (QIAGEN). We then used SAMtools v1.3 to sort the aligned bam files and generate alignment statistics (WysokerA ...
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bioRxiv - Immunology 2021Quote: DNA was isolated from 1 × 106 PBMC using the DNeasy Blood & Tissue Kit (Qiagen, Cat No 69506), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... cDNA synthesis was performed with 1 mg of total RNA using miScript II Reverse Transcription Kit (QIAGEN). See Supplemental Information for primers used in these experiments ...
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bioRxiv - Cell Biology 2021Quote: ... SF9 cells at 1×106 SF9 cells/ml were grown in 24 well round bottom plates (Qiagen) and infected with baculovirus of the indicated single subunits ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from 1 × 108 trypanosome cells using the Qiagen RNeasy Mini Kit (Qiagen, Netherlands) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μl diluted cDNA (1:20) were added to SYBR Green PCR Master Mix (Qiagen, Hilden, Germany) and 10 μM of both forward and reverse primer (Primer Sequences Table 1) ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then vortexed briefly and 1 mL of ice-cold QIAzol Lysis Reagent (Qiagen, cat. 79306) was added to each sample ...
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bioRxiv - Microbiology 2020Quote: ... amplicons were purified from excised gel slices (1% agarose) using the Qiagen MinElute Gel Extraction kit (Qiagen). Each amplicon was quantified using Quant-IT HS reagents (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The soluble fraction was incubated for 1 hour at 4 °C using a Ni-NTA resin (Qiagen). The Ni-NTA resin was washed with 4 times resin volume (RV ...
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bioRxiv - Microbiology 2021Quote: ... The samples were homogenized for 2 × 1 min at 30 Hz using a TissueLyser (Qiagen, Hilden, Germany) and stored at -20 °C until further processing.
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bioRxiv - Microbiology 2021Quote: ... stored at −20° C and characterized by immnoblotting using mouse anti-2xStrep tag (Qiagen #34850, 1:1,000), and secondary goat anti-mouse IgG IRDye® 800CW (LI-COR # 926-32210 ...
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bioRxiv - Microbiology 2021Quote: ... Five hundred microliters of exponentially growing cells were stabilized in 1 mL of RNAprotect Bacteria Reagent (Qiagen). After treatment of the culture pellet with lysozyme (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: The obtained ~2Kb amplicons were gel purified from 1% agarose gel using QIAquick Gel Extraction Kit (Qiagen) and cloned in pBluescript SK (+ ...
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bioRxiv - Neuroscience 2020Quote: The obtained ~2Kb amplicons were gel purified from 1% agarose gel using QIAquick Gel Extraction Kit (Qiagen) followed by clean-up with QIAquick PCR purification kit (Qiagen) ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... were added to each sample tube together with 400μL a 1% β-mercaptoethanol in RLT buffer (Qiagen) and 2μL of antifoam agent (ID 19088 ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of 1 μg of RNA were reverse transcribed using a QuantiTect Reverse Transcription kit (Qiagen, 205314), and qPCR was performed with iTaq SYBR Green (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... at a ratio of 1:0.6 with a final elution in 25 μl of EB Buffer (Qiagen). The cDNA was then quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1-2 µg of RNA was used for the cDNA synthesis (Omniscript RT Kit (Qiagen, 205111)) ...
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted from skin biopsies (~1 cm2) taken from the upper backs of mice (Qiagen #74106). Each RNA sample was collected in 40 μL of ultrapure water (Thermo Fisher #10977) ...
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bioRxiv - Molecular Biology 2022Quote: ... including of multiple steps showed below: 1) Total DNA was extracted using QIAamp DNA Mini Kit (Qiagen); 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... 1000:1 v/v) and DNA from individual colonies were isolated using QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Cell Biology 2022Quote: ... and passed through 1 mL of glutathione–Sepharose 4B and then onto a Ni-NTA column (QIAGEN) to capture the residual GST- and His-tag fragments ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of a 10 min bead beating step at 50 s−1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure-Bacteria kit protocol (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation and supernatant was mixed with 1 ml Ni-NTA Agarose (Qiagen) and incubated for 90 minutes at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2021Quote: ... Frozen lung sections were homogenized in 1 ml of Lysis/Binding solution in a TissueLyser II (Qiagen) with three cycles of at 30 Hz for 30 seconds ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were performed in a final volume of 20 μl containing Hot Start Taq (1 U, QIAGEN), 3.5 mM MgCl2 ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from stably transduced THP-1 cultures using the RNeasy Plus Mini kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and a Cyprus wheatear (O. cypriaca) (Table 1) using the MagAttract HMW DNA kit (Qiagen, Hilden, Germany). Digestion was performed in 360 ul of buffer ATL ...
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bioRxiv - Immunology 2020Quote: ... in purity mode into 96-well microplates containing 10 μL of 1% 2-mercaptoethanol RLT buffer (Qiagen) and stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was mixed with 1 ml of 50% Ni2+-NTA His-bind resin (Qiagen, Hilden, Germany), incubated for 1 hr at 4°C with mild rotatory shaking ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Immunology 2020Quote: ... All steps adhered to the manufacturer’s instructions and included a DNase 1 treatment (RNase-Free DNaset, Qiagen) step to remove genomic DNA contamination ...
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bioRxiv - Neuroscience 2020Quote: ... DNA was isolated from 1-2 mm tail tissue using the DNeasy Blood and Tissue Kit (Qiagen) following the protocol for isolation of DNA from animal tissues and was eluted in 100 µl H2O ...
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bioRxiv - Immunology 2020Quote: ... Isolation of RNA from THP-1 cells was carried out with a RNeasy Plus Mini kit (Qiagen). The High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... In brief we perform bisulfite conversion on 1 µg of genomic DNA using EpiTech kit from Qiagen according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Purification was performed by incubating the cleared lysate with 1 mL Ni-NTA resin (Qiagen, Hilden, Germany) on a rotating platform at 4°C for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5ug of the plasmid of choice was transfected into HEK293T cells together with 1 ug pVSV-G and 1.5 ug pCMVΔR8.2 using an Effectene transfection kit (Qiagen). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... The siRNA oligonucleotides targeting Arf1 and IRSp53 were purchased from Qiagen (Flex-iTube GeneSolution GS375 for Arf1; siArf1#1 Cat. No. SI02654470; siArf1#2 Cat. No. SI00299250; Qiagen Flex-iTube IRSp53 siRNA#1 Cat ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting products were run on 1 % agarose gel then extracted using QIAquick Gel Extraction Kit (Qiagen). Restriction enzymes and Phusion DNA polymerase were purchased from New England BioLabs Inc and used according to the manufacturer’s instructions ...