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Citations for Qiagen :
2051 - 2100 of 2825 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from approximately 1 million cells per condition using DNA extraction kit (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were loaded onto a 1 mL Ni-NTA Superflow column (Qiagen, Valencia, CA, USA) that had been equilibrated with 10 column volumes of wash 1 buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant proteins were purified on Ni-NTA columns (1 ml Ni-NTA Agarose; Qiagen Cat # 30210), washed with 20 ml hexokinase buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria (∼1 × 109 cells) were removed from the cultures and suspended in RNAprotect bacteria reagent (Qiagen), pelleted by centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from 1 ml of overnight culture (DNeasy Blood and Tissue Kit—Qiagen) pre-treated with 100 μg of lysostaphin (Sigma cat ...
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bioRxiv - Microbiology 2023Quote: ... These were harvested by centrifugation and resuspended in 1 mL of RNA Protect Bacteria Reagent (Qiagen) with 30µL of 20 mg/mL lysozyme ...
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bioRxiv - Molecular Biology 2024Quote: Wild-type or mutant SMYD5 was purified with either 1 mL Ni-NTA Superflow Cartridges (Qiagen) or His Mag Sepharose Ni resin (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: Genomic DNA was extracted from 1 million cells using the Qiamp DNA mini kit (QIAGEN, #51304) following manufacturer instructions and DNA concentration was measured with Nanodrop 2000c (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... 1% v/v sodium dodecyl-sulfate pH 8.0) for protein extraction or in RLT buffer (Qiagen) for RNA extraction ...
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bioRxiv - Systems Biology 2024Quote: ... 1 μg of RNA per experimental sample was processed with the QuantiTect cDNA Synthesis Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were generated using 1 ug of the total RNA via QIAseq miRNA kit (Qiagen, # 331502) with 14 cycles of amplification ...
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bioRxiv - Molecular Biology 2024Quote: Small interfering RNA (siRNA) to knockdown TSP-1 expression in HUVECs was obtained from Qiagen (GS7057). AllStars siRNA was used as negative control ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were generated using 1 µg of the total RNA via QIAseq miRNA kit (Qiagen, # 331502) with 14 cycles of amplification ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from 1 × 106 cells using the RNeasy Plus Mini Kit (Qiagen; 74134), and cDNA was synthesized with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... DNA fragments were purified from a 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen). Barcoded TruSeq adapters were added to the mutant fragments by PCR (7 cycles ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA (gDNA) was extracted from 1-5×106 cells using DNeasy Blood and Tissue Kit (Qiagen) and 250 ng gDNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was recovered using a chloroform-ethanol procedure (Supplementary protocol 1) and diluted in AE buffer (Qiagen). A pair-ended library was constructed by Eureka Genomics (Hercules ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted from 1 ml of overnight culture using a QIAamp DNA Mini Kit (Qiagen). Illumina MiSeq sequencing operated by the Functional Genomics Centre Zurich and Novogene (Cambridge ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... and also performed direct mapping against reference genome Wuhan/Hu-1/2019 (NCBI Nucleotide – NC_045512, GenBank – MN908947) using the CLC Genomics Suite v5.1.0 (QIAGEN). We then used SAMtools v1.3 to sort the aligned bam files and generate alignment statistics (WysokerA ...
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bioRxiv - Immunology 2021Quote: DNA was isolated from 1 × 106 PBMC using the DNeasy Blood & Tissue Kit (Qiagen, Cat No 69506), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... cDNA synthesis was performed with 1 mg of total RNA using miScript II Reverse Transcription Kit (QIAGEN). See Supplemental Information for primers used in these experiments ...
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bioRxiv - Cell Biology 2021Quote: ... SF9 cells at 1×106 SF9 cells/ml were grown in 24 well round bottom plates (Qiagen) and infected with baculovirus of the indicated single subunits ...
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bioRxiv - Synthetic Biology 2021Quote: mESC gDNA was purified from 1-5 million cells using the QIAamp DNA mini kit (Qiagen 51306) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from 1 × 108 trypanosome cells using the Qiagen RNeasy Mini Kit (Qiagen, Netherlands) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then vortexed briefly and 1 mL of ice-cold QIAzol Lysis Reagent (Qiagen, cat. 79306) was added to each sample ...
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bioRxiv - Microbiology 2020Quote: ... amplicons were purified from excised gel slices (1% agarose) using the Qiagen MinElute Gel Extraction kit (Qiagen). Each amplicon was quantified using Quant-IT HS reagents (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The soluble fraction was incubated for 1 hour at 4 °C using a Ni-NTA resin (Qiagen). The Ni-NTA resin was washed with 4 times resin volume (RV ...
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bioRxiv - Microbiology 2021Quote: ... stored at −20° C and characterized by immnoblotting using mouse anti-2xStrep tag (Qiagen #34850, 1:1,000), and secondary goat anti-mouse IgG IRDye® 800CW (LI-COR # 926-32210 ...
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bioRxiv - Microbiology 2021Quote: ... Five hundred microliters of exponentially growing cells were stabilized in 1 mL of RNAprotect Bacteria Reagent (Qiagen). After treatment of the culture pellet with lysozyme (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: The obtained ~2Kb amplicons were gel purified from 1% agarose gel using QIAquick Gel Extraction Kit (Qiagen) and cloned in pBluescript SK (+ ...
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bioRxiv - Neuroscience 2020Quote: The obtained ~2Kb amplicons were gel purified from 1% agarose gel using QIAquick Gel Extraction Kit (Qiagen) followed by clean-up with QIAquick PCR purification kit (Qiagen) ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... were added to each sample tube together with 400μL a 1% β-mercaptoethanol in RLT buffer (Qiagen) and 2μL of antifoam agent (ID 19088 ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of 1 μg of RNA were reverse transcribed using a QuantiTect Reverse Transcription kit (Qiagen, 205314), and qPCR was performed with iTaq SYBR Green (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... at a ratio of 1:0.6 with a final elution in 25 μl of EB Buffer (Qiagen). The cDNA was then quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted from skin biopsies (~1 cm2) taken from the upper backs of mice (Qiagen #74106). Each RNA sample was collected in 40 μL of ultrapure water (Thermo Fisher #10977) ...
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bioRxiv - Molecular Biology 2022Quote: ... including of multiple steps showed below: 1) Total DNA was extracted using QIAamp DNA Mini Kit (Qiagen); 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... 1000:1 v/v) and DNA from individual colonies were isolated using QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Cell Biology 2022Quote: ... and passed through 1 mL of glutathione–Sepharose 4B and then onto a Ni-NTA column (QIAGEN) to capture the residual GST- and His-tag fragments ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of a 10 min bead beating step at 50 s−1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure-Bacteria kit protocol (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation and supernatant was mixed with 1 ml Ni-NTA Agarose (Qiagen) and incubated for 90 minutes at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2021Quote: ... Frozen lung sections were homogenized in 1 ml of Lysis/Binding solution in a TissueLyser II (Qiagen) with three cycles of at 30 Hz for 30 seconds ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were performed in a final volume of 20 μl containing Hot Start Taq (1 U, QIAGEN), 3.5 mM MgCl2 ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from stably transduced THP-1 cultures using the RNeasy Plus Mini kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and a Cyprus wheatear (O. cypriaca) (Table 1) using the MagAttract HMW DNA kit (Qiagen, Hilden, Germany). Digestion was performed in 360 ul of buffer ATL ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was mixed with 1 ml of 50% Ni2+-NTA His-bind resin (Qiagen, Hilden, Germany), incubated for 1 hr at 4°C with mild rotatory shaking ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Immunology 2020Quote: ... All steps adhered to the manufacturer’s instructions and included a DNase 1 treatment (RNase-Free DNaset, Qiagen) step to remove genomic DNA contamination ...
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bioRxiv - Immunology 2020Quote: ... Isolation of RNA from THP-1 cells was carried out with a RNeasy Plus Mini kit (Qiagen). The High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... In brief we perform bisulfite conversion on 1 µg of genomic DNA using EpiTech kit from Qiagen according to the manufacturer’s instructions ...