Labshake search
Citations for Qiagen :
1951 - 2000 of 2329 citations for 8 3 5 DIMETHYL 4 METHOXYPHENYL 8 OXOOCTANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: For RT product measurements DNA was extracted from 5×105 infected cells using the DNeasy Blood & Tissue kit (QIAgen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... then PCR-amplified using the PyroMark PCR kit using Qiagen’s pyrosequencing primer assays or those designed in-house (Suppl. Table 5) via the PyroMark Assay Design Software 2.0 (Qiagen). Reaction conditions were as follows ...
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bioRxiv - Microbiology 2021Quote: ... was performed by using 2.5 μL of the synthesized and diluted cDNA in the presence of 5 μL QuantiTect SYBR Green (204143, Qiagen) and 1 mM specific primers (S6 Table ...
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bioRxiv - Cell Biology 2021Quote: RNA from 5-10 million NK cells treated overnight with indicated conditions was extracted using Rneasy Plus kit (Qiagen). Samples were prepared for Illumina sequencing following the manufacturer’s protocol using the TruSeq® stranded total RNA with rRNA depletion using Ribo Zero TM Human/Mouse/Rat ...
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bioRxiv - Synthetic Biology 2021Quote: ... the biofilms were disrupted by pipetting thoroughly and homogenized with a stainless bead beater 5 mm in diameter (Qiagen) for 10 min at a frequency of 30 beats per second by TissueLyser II (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... 100 μl of total RNAs were extracted from 5 × 105 replicon cells using RNeasy mini kit (Qiagen, Gaithersburg, MD). 2□μl of each sample was used to assess RNA quality using Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 g for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... For RT product measurements DNA was extracted from 5×105 infected cells using the DNeasy Blood & Tissue kit (QIAgen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA were extracted from roots 5 days after germination using the RNeasy® Plant Mini kit (Qiagen #74904) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... protein yields of the CNA35 probe were synthesized using E.Coli bacteria before purification using ÄKTApurifier (Cytiva) and a 5 ml Ni-NTA Superflow Cartridge (Qiagen), dialysis with SnakeSkinTM Dialysis tubing with 10 kDa MWCO ...
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bioRxiv - Cancer Biology 2022Quote: ... each well was incubated in 75 μL 0.08 M KOH for 5 min and washed with 100 μL EB buffer (QIAGEN) at room temperature (RT) ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysates were then incubated at 95°C for 5 min and filtered using a QIAshredder homogenizer (Qiagen, 79656). The extracted proteins were analyzed using 5–20% gradient sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... bacterial pellets were resuspended and incubated at room temperature for 5 mins in 500µl RNAprotect® bacteria reagent (QIAGEN). Samples were centrifuged at 16000g for 5 mins and supernatant removed ...
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bioRxiv - Microbiology 2022Quote: ... and microbial Eukaryotic abundance with a QIAcuity One 5-plex digital PCR (dPCR) instrument (Qiagen Inc. USA, Germantown, MD). dPCR samples represented both the 416 Fire —across SBS ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA from 1-5 million total CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen) or phenol-chloroform and the DNA concentration was measured by Qubit High Sensitivity Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The homogenates were clarified by centrifugation at 9,000 × g for 5 min and RNA was extracted using RNeasy Mini Kit (Qiagen) following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... Tissue samples (5-25 mg) were kept on ice and submerged in RNAlater RNA stabilization reagent (QIAGEN, Hilden, Germany) within 30 minutes of collection and stored at −80°C.
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bioRxiv - Microbiology 2020Quote: ... 5 μl of cDNA was then added to a PCR mix containing 12.5 μl 2× Multiplex PCR mix (Qiagen), 9 μl H2O ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... of vehicle and Acyl-GIP vehicle treated LDLR−/− mice (n = 5) using Qiazol according to the manufacturer’s instructions (Qiazol Lysis Reagent, QIAGEN). The quality of the RNA was determined with the Agilent 2100 BioAnalyzer (RNA 6000 Nano Kit ...
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The mitochondrial genome of the red icefish (Channichthys rugosus) casts doubt on its species statusbioRxiv - Zoology 2022Quote: ... the lysates were centrifuged at 17,000 x g for 5 minutes and 300 μL supernatant mixed with 3000 μL Buffer PB (Qiagen). The mixtures were loaded onto Minelute silica columns (Qiagen ...
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bioRxiv - Physiology 2020Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was isolated after disrupting worms with 5 freeze-thaws with Qiazol extraction and RNeasy mini kit (Qiagen). cDNA libraries were prepared through TruSeq Stranded mRNA kit (Illumina ...
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bioRxiv - Physiology 2021Quote: ... the supernatant was harvested by centrifugation for 20 min at 10,000 g and then loaded onto 5 ml Immobilized Metal-ion Affinity chromatography (IMAC, Ni-NTA Agarose, Qiagen) pre-equilibrated in 20 mM Hepes pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... Epithelial tissue and chorion were individually sampled from 3 to 5 consecutive sections and each microdissected tissue was immediately placed in 80μL of ice-chilled RLT buffer (Qiagen) and stored at −20°C until RNA extraction ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from 5 million transfected Jurkat T-cells using the DNeasy Blood and Tissue kit (Qiagen) and quantified by Quant-iT™ PicoGreen™ dsDNA Assay kit (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were homogenized at 25–30 Hz for 5 min using a tissue homogenizer (QIAGEN TissueLyzer II, Hilden, Germany). Samples were swabbed with sterile dual tip swabs (BD swubes ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4C ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4°C ...
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bioRxiv - Biochemistry 2021Quote: The LRH-1-10CA-TIF2 complex was concentrated to 5 mg/mL and screened using the Classics screen (Qiagen) and a Phoenix Liquid Handler (Art Robbins Instruments ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with siRNA as above (Lap2: 5’-GAUGUGACAGAGCUCUCUA from Sigma; negative control: AllStars negative control from Qiagen). Total RNA was extracted with a Nucleospin RNA kit from Macherey-Nagel according to the manufacturer’s protocol from triplicates of control and Lap2-depleted samples ...
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bioRxiv - Immunology 2021Quote: ... viable in vitro differentiated CD4+ T cells were sorted into 96-well plates containing 5 μl TCL Buffer (QIAGEN) with 1% 2-mercaptoethanol ...
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bioRxiv - Immunology 2020Quote: ... which contained 5 μl of sort buffer consisting of (1X Qiagen One-step RT PCR Buffer (Qiagen, Hilden, Germany), 0.1 mM dithiothreitol (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Enriched cells were surface stained and 5-20 ×103 intratumoral cDC1s were sorted directly in RLT lysis buffer (Qiagen) containing 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... Transcribed cDNA was diluted 5-fold and used for real-time quantitative PCR (QuantiTect SYBR Green; Qiagen ref: 204145). RNA-sequencing libraries were produced with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (E7770) ...
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bioRxiv - Developmental Biology 2022Quote: Ovarian total RNA was extracted from 5 μl freshly dissected fly ovaries by using Qiagen RNeasy Purification Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was loaded onto a 10 mL Ni-NTA Superflow cartridge (connected two 5 mL cartridges) (30761, Qiagen). The cartridge was washed with 100 mL R buffer containing 200 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble fraction was recovered by centrifugation at 40 000 × g in a JLA25.50 rotor and put through a gravity flow column with 5 ml of NiNTA Agarose (Qiagen). Bound fraction was washed in Buffer B (300 mM NaCl ...
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bioRxiv - Genetics 2023Quote: ... medium was removed using the Bluewasher (BlueCatBio) and cells were lysed for 5 min using RLT plus buffer (Qiagen), snap frozen on dry ice and stored at −80 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR mix contained 0.16 µM of each of the fluorescent universal primer and of the reverse specified primer and 0.04 µM of the 5’ tail forward primer in a final 15 µl reaction volume (2x QIAGEN Multiplex PCR Master Mix with 3 mM Mg2+ ...
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bioRxiv - Microbiology 2024Quote: ... Total cellular DNA was extracted from approximately 0.5 to 5 million cells using a QIAamp DNA Blood Minikit (Qiagen). PCR assays were performed with 200 ng samples of DNA ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from approximately 5-10 million cells from each organ using the RNeasy Mini Kit (QIAGEN). SuperScript® IV Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer in a 50 mL falcon tube ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was filtered through a 0.45 µm syringe filter and applied to a 5 ml Ni-NTA agarose column (Qiagen) pre-equilibrated in Lysis-Wash buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were filtered through a 0.22 µm syringe filter before application to 5 ml of nickel resin (Ni-NTA Superflow, QIAGEN) equilibrated in loading buffer (25 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... The filtrated supernatant containing His-thioredoxin-ApoE was applied to a 5 ml Ni-NTA Superflow Cartridge (Qiagen, Germany) pre-equilibrated with TBS buffer A ...
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bioRxiv - Immunology 2023Quote: ... 14-666-315) containing 1 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Nucleic acids were isolated from excised granulomas by homogenizing in TRIzol (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... and 20 ng cDNA were subjected to the Rotor-Gene Q 5-Plex HRM real-time PCR system (Qiagen), using the default program ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 5 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Biochemistry 2023Quote: ... The sonicate was centrifuged at 38,000 × g for 30 min and the supernatant was loaded on a Ni-NTA agarose column (2.5 × 5 cm, Qiagen) pre-equilibrated with buffer (50 mM Tris-HCl ...
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bioRxiv - Genomics 2023Quote: ... VDAC1P8-201 and GAPDH (Suppl. Table 5) and 12.5 μl of master mix (QuantiFast SYBR Green PCR kit, Qiagen). Analysis of relative expression level was performed using the housekeeping GAPDH gene as internal calibrator by the ΔΔCt method ...