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Citations for Qiagen :
151 - 200 of 2389 citations for T complex protein 1 subunit epsilon CCT5 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Genomic DNA was extracted from T cells using a DNeasy Blood & Tissue kit (Qiagen, Cat no. 69506). PCR amplification of the region of gDNA containing the CRISPR editing locus was performed using the SequalPrep Long PCR Kit with dNTPs (Invitrogen ...
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bioRxiv - Immunology 2021Quote: DNase I-treated total RNA was prepared from sorted T cells using RNeasy RNA isolation kit (Qiagen) and cDNA was synthesized using an iScript cDNA synthesis kit (Biorad) ...
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bioRxiv - Cancer Biology 2022Quote: CD4+ and CD8+ T cells were collected on the FACS Aria Fusion into RNAse free eppendorfs (Qiagen), transported on ice and cell pellets were resuspended and lysed in RLT buffer (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from isolated T-lymphocytes using the RNAeasy mini kit (Qiagen #74104, Valencia, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from in vitro activated CD4+ T cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was isolated from a median of 3.65 million CD4+ T cells (IQR 2.48-4.98) with the DNeasy Blood & Tissue Kit (QIAGEN). Each HIV reaction contained 825 ng of gDNA ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA of CAR-T products was extracted using DNeasy Blood and Tissue Kit (QIAGEN, Germantown, MD) and randomly fragmented (<500 bp fragments ...
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bioRxiv - Immunology 2024Quote: RNA was extracted from 5 × 106 T cells using the RNeasy® Mini Kit (Qiagen, Cat. 74106). RNA extraction was performed following the instructions from the “Purification of Total RNA from Animal Cells Using Spin Technology” protocol given in the RNeasy® Mini Handbook ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted from naïve or activated CD8+ T cells using RNeasy Kit (Qiagen, catalog number: 74104) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Molecular Biology 2021Quote: The solubilized protein was incubated for 1 hour with washed and pre-equilibrated Strep-Tactin Superflow plus resin (Qiagen). The resin was then loaded into an Econo-Column Chromatography Column (Biorad ...
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bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
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bioRxiv - Genomics 2023Quote: ... Button valves were opened and biotinylated anti-pentaHis antibody (Qiagen, 1:4 dilution in HEPES) was flowed for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was extracted 48 hours post transfection with All Prep RNA/Protein Kit (Qiagen, USA). Protein concentrations were determined by Lowry assay (Bio-Rad ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... protein extraction was performed using the Allprep RNA/Protein Kit (80404 Qiagen Inc., Hilden, Germany). Proteins (10–20 µg ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein extraction were performed according to manufacturer instructions (AllPrep DNA/RNA/Protein minikit; Qiagen). Each spin column flowthrough (DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was isolated from powdered tissues using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Protein pellets were lysed in HES-SDS buffer (20 mM HEPES [pH 7.4] ...
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bioRxiv - Cell Biology 2023Quote: Cells were collected for total protein using Qproteome Mammalian Protein Prep Kit (Cat. 37901, Qiagen). Lysates were quantified for protein expression utilizing the WES system (ProteinSimple ...
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bioRxiv - Microbiology 2023Quote: ... The His-tagged proteins were purified using the QIAexpress Ni-NTA Protein Purification System (Qiagen) with 0.1 M ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from FACS/MACS-isolated CD8+ T cells and reverse-transcribed using RNeasy Micro Kit (Qiagen) and SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2021Quote: Total RNA was collected from 2×106 CAR T cells with the RNEasy Plus Mini isolation kit (Qiagen). Library preparation and RNA-seq was performed by BGI America (Cambridge ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from T-ALL cells collected purification using RNeasy Plus Mini or Micro Kit (Qiagen). cDNA were generated using SuperScript® VILOTM cDNA Synthesis Kit (ThermoFisher Scientific).Real-time PCR reactions were carried out using Power SYBR® Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from 0.5×106 naïve and activated CD8+ T cells using a using an RNeasy Mini kit (Qiagen) and additionally purified using an RNase Free DNase Set (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... and DNA and RNA were extracted from CD4+ T cells using the AllPrep Universal Kit (Qiagen, Hilden, Germany). Cell-associated total HIV DNA and unspliced RNA were quantified by an in-house quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Immunology 2023Quote: ... These purified CAR-T cells were lysed for bulk RNA extraction using RNeasy Plus mini isolation kit (Qiagen). Library preparations were performed using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: RNA isolation of platelet releasate treated MHEC5-T cells was performed using the RNeasy Mini Kit (#74106; Qiagen). The isolation was conducted according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The RT-qPCR experiments used Student’s t-test of the experimental group compared to the sham control (Qiagen GeneGlobe RT2 Profiler PCR Data Analysis) ...
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bioRxiv - Immunology 2023Quote: RNA was purified from at least 5 x 104 CD4+ T cells using the RNeasy Micro kit (Qiagen). cDNA was synthesized using the SuperScriptTMVILOTMcDNA synthesis kit (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: Total RNA from isolated T cells and colon tissue was isolated using the RNeasy Mini Kit (Qiagen, Germany). cDNA was then generated from 200 ng total RNA using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: Genomic DNA was isolated from 1E6 T cells using the QIAamp DNA Mini Kit (Qiagen, Cat-no. 51304). After PCR ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed 3 times with sterile 1x PBS and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed four times.
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bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
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bioRxiv - Microbiology 2023Quote: ... The protein concentrations of all the collected samples were adjusted to 400 μg mL−1 using the Qubit protein broad range assay kits (Qiagen). The Cyclic AMP XP® kit ELISA plates were prepared according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using mouse anti-His antibody from Qiagen (Cat No./ID: 34660 1:500). Anti-mouse HRP was used for colorimetric development (Cat No./ID-A8924 1:1000) ...
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bioRxiv - Plant Biology 2020Quote: ... The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen) and analyzed by SDS-PAGE.
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified using the His-tag Protein Purification Kit (Ni-NTA Agarose, QIAGEN GmbH), GST-tag Protein Purification Kit (Glutathione Sepharose ...
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bioRxiv - Molecular Biology 2023Quote: Total proteins from P5 hippocampi were purified with AllPrep DNA/RNA/Protein Mini Kit (Qiagen, 80004). The protein pellets were then resuspended in 90 μl Buffer ALO (Qiagen ...
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bioRxiv - Physiology 2020Quote: ... protein was extracted (Qiagen Tissue Lyser) from ∼50 mg frozen liver in tissue lysis buffer 80 ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL Protein Precipitation Solution (QIAGEN) was added ...
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bioRxiv - Cancer Biology 2023Quote: Advanced Protein Purification buffer (APP; Qiagen): the APP buffer contains zinc chloride to precipitate protein at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA and proteins were recovered by using the Allprep DNA/RNA/Protein Mini Kit (Qiagen 80004). The efficiency of Cre-induced recombination in Appflox/flox mice was verified by PCR with primers F ...
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bioRxiv - Neuroscience 2020Quote: ... Protein and RNA extraction was done using Qiagen AllPrep DNA/RNA/Protein isolation kit (Qiagen Inc, USA). RNA purity was confirmed with 260/280 OD ratio using Nanodrop reader (Thermo Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... some samples were lysed for total protein extraction using Qproteome Mammalian Protein Prep Kit (Qiagen, Hilden, Germany). Western blot was done using capillary electrophoresis based system Wes™ (ProteinSimple ...
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bioRxiv - Biochemistry 2024Quote: ... co-expressing lambda protein phosphatase and purified with the QIAexpress Ni-NTA Protein Purification System (Qiagen, 30210) according to the manufacturers protocol ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... total RNA was extracted from purified splenic T-lymphocytes utilizing the RNAeasy mini kit (Qiagen #74104, Valencia, CA, USA) prior to conversion to cDNA (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 1 μg of a pcDNA3.1 vector containing human αSyn cDNA (provided by T. Baron, Anses, Lyon, France) using Effecten transfection reagent (Qiagen). Positive clones were selected using geneticin and cultured in Dulbecco’s modified Eagle’s medium (DMEM/F-12 ...
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bioRxiv - Immunology 2022Quote: ... TCRαβ+CD4-CD45RClow/- T cells from Il34+/+ and Il34-/- rats were extracted using RNeasy Mini Kit (QIAGEN, Hilden, Germany) and protocol of 3’ DGE RNA sequencing was performed as previously described (16) ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from 5 million transfected Jurkat T-cells using the DNeasy Blood and Tissue kit (Qiagen) and quantified by Quant-iT™ PicoGreen™ dsDNA Assay kit (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: Between 104 and 3.104 murine and human T cells were sorted from lymph nodes and tumors in TCL buffer (Qiagen) with 1% of β-mercaptoethanol ...