Labshake search
Citations for Qiagen :
151 - 200 of 1421 citations for Diazinon Unlabeled 1000 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... 20 µg/mL RNase A (QIAGEN, #19101), 1 tablet EDTA-free protease inhibitor (Roche ...
-
bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
-
bioRxiv - Molecular Biology 2020Quote: ... The reaction was incubated for 30 min at 37 °C with mixing (1000 r.p.m.) and directly subjected to DNA purification using the MinElute Reaction Cleanup Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The samples were centrifuged and the supernatant mixed with 13 mL of a modified PB buffer (12.6 mL PB buffer (Qiagen), 6.5 μL Tween-20 ...
-
bioRxiv - Plant Biology 2021Quote: ... the protein was removed by 1.1 μL of proteinase K (20mg/ml) at 45° C for 1h and RNA by 2μL of RNaseA (1mg/ml) (Qiagen) digestion at 37°C for 1h ...
-
bioRxiv - Molecular Biology 2020Quote: ... washed twice in PBS and DNA was labeled with propidium iodide (50 μg/ml) in presence of RNase A (0.2 mg/ml, Qiagen) and Triton X-100 (0.1%) ...
-
bioRxiv - Microbiology 2019Quote: ... Bacteria were treated with lysostaphin (2.5 mg/mL) and lysozyme (50 mg/mL) prior to RNA extraction using the RNeasy Plus Mini Kit (Qiagen). RNAs were treated with TURBO DNA-free™ (Invitrogen) ...
-
bioRxiv - Immunology 2020Quote: ... were homogenised in a Precellys 24 homogeniser with CK28 Hard tissue homogenizing 2.0 ml tubes (Bertin) and 1 ml of RLT buffer (Qiagen) supplemented with 1%(v/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... and DNA was labeled with propidium iodide (50 μg/ml) in the presence of RNase A (0.2 mg/ml, Qiagen) and Triton X-100 (0.01% ...
-
bioRxiv - Molecular Biology 2022Quote: ... The supernatant was loaded onto a 10 mL Ni-NTA Superflow cartridge (connected two 5 mL cartridges) (30761, Qiagen). The cartridge was washed with 100 mL R buffer containing 200 mM NaCl ...
-
bioRxiv - Microbiology 2023Quote: ... Cells suspended in lysis buffer were transferred to a 2 mL screw cap tube that contained 0.5 mL of 0.1 mm disruption beads for bacteria and 0.7 mL QIAzol™ Lysis Reagent (Qiagen; 79306). Cells were lysed via mechanical disruption using a bead beater for four rounds of 30 s each ...
-
bioRxiv - Systems Biology 2023Quote: ... A culture volume equal to 3 mL of OD = 1 was added to 6 mL RNAprotect Bacteria Reagent (Qiagen), vortexed ...
-
bioRxiv - Immunology 2019Quote: ... longitudinal muscle/myenteric plexus preparations were homogenized in a 2 ml eppendorf containing 1 ml Trizol and a single 5 mm steel bead in a TissueLyzer (Qiagen) for 3 min at 30 Hz ...
-
bioRxiv - Immunology 2019Quote: ... mucosal scrapings and feces were homogenized in a 2 ml eppendorf containing 1 ml of DNA extraction buffer and a single 5 mm steel bead in a TissueLyzer (Qiagen) for 3 min at 30 Hz ...
-
bioRxiv - Microbiology 2021Quote: ... 1 g yeast extract in 750 ml of 30 kDa filtered seawater and 250 ml of Milli-Q water) using the DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2021Quote: The supernatant was carefully removed to a 50-mL sterile tube and mixed with 2 mL volume of 50% Ni-NTA resin (Qiagen) which was pre-equilibrated in the buffer A ...
-
bioRxiv - Microbiology 2020Quote: ... and the cycle-threshold CT translated to bacterial load (estimated CFU per mL (eCFU/mL) using a standard curve on a Rotor gene Q 5plex platform (Qiagen). The cut-off for TB-MBLA positivity is a 30 CT value that corresponds to 1.0 log10 eCFU/mL ...
-
Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... Transcripts were stabilized by mixing 3 mL of cell cultures at the mid-log phase with 6 mL of RNAprotect Bacteria Reagent (Qiagen). Samples were immediately vortexed for 5 sec ...
-
bioRxiv - Microbiology 2022Quote: ... from the top A horizon were immediately collected for DNA extractions in 15 mL tubes containing 5 mL Lifeguard Soil Preservation Solution (QIAGEN) using chilled soil-processing trays ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmids were transformed into JM109 chemically competent cells and grown overnight in 250 mL of LB broth with 100 µg/mL of ampicillin and purified using the HiSpeed Plasmid Maxi Kit (Qiagen).
-
bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was transferred to a 15 ml falcon tube and 1 ml of a 50% slurry consisting of NiNTA agarose beads (Qiagen) in resuspension buffer was added per 10 ml of lysate ...
-
bioRxiv - Synthetic Biology 2019Quote: ... grown over night in 5 mL LB and 100 μg mL-1 ampicillin and DNA were purified using a QIAprep Spin Miniprep Kit (QIAGEN). Sequencing was performed by GATC using the primers in SI Appendix Table S4 ...
-
bioRxiv - Immunology 2020Quote: ... Supernatants were removed and the remaining cell pellet (500 μl) was transferred into a 15 ml tube containing 2.5 ml RNAprotect® Cell Reagent (Qiagen). The cells were resuspended by vortexing ...
-
bioRxiv - Microbiology 2021Quote: ... Mosquito bodies and legs and wings were put into a 2 ml round-bottom tubes containing 250 ml of PBS and a steel ball (Qiagen). Samples were homogenized using a TissueLyser (Qiagen ...
-
bioRxiv - Immunology 2020Quote: ... in 96 flat bottom plates in the presence or absence of PMA (200ng/ml) and ionomycin (300ng/ml) were lysed in 100Sµl lysis buffer containing TCL buffer (Qiagen, 1031576) containing 1% β-mercaptoethanol ...
-
bioRxiv - Molecular Biology 2021Quote: ... clones were isolated from a 5 ml High Salt (HS) Luria Bertani (LB) culture containing ampicillin (50 μg/ml) using the QIAprep Spin Miniprep Kit (Qiagen). Inserts in pGEM-T Easy were sequenced at Aberystwyth University’s Translational Genomics Facility and the sequence trace was analysed using FinchTV 1.4.0 software [45] ...
-
bioRxiv - Biochemistry 2021Quote: ... and early stationary phase (OD = 1.0-1.3).1 mL sample of each culture was directly transferred to 2 mL RNAprotect cell reagent (Qiagen, Hilden, Germany). The samples were vortexed 5 sec ...
-
bioRxiv - Biophysics 2023Quote: ... and precipitated with ammonium sulfate as in Niu et al.49.The precipitate was dissolved in 30 ml buffer C and loaded onto a 5 ml column of Ni-NTA-agarose (Qiagen) pre-equilibrated in buffer C ...
-
bioRxiv - Molecular Biology 2023Quote: ... fungal DNA was extracted independently from 1 mL of crude conidial suspensions (between 108 and 109 spores mL-1) using the DNeasy 96 Plant Kit (Qiagen) following the manufacturer’s instructions (Gautier et al. ...
-
bioRxiv - Microbiology 2023Quote: ... Pellets were washed in 2 mL cold 10 mM NaCl + 4 mL cold RNAprotect Bacteria Reagent (Qiagen Cat. No. 76506) and repelletted at 4,255 x g ...
-
bioRxiv - Systems Biology 2023Quote: ... Samples were collected continuously into 50 mL conicals containing 20 mL RNAlater (saturated ammonium sulfate, 20mM EDTA, pH 5.2; QIAGEN #76106) and a stirring magnetic stir disc (V&P Scientific #772DP-N42-5-2) ...
-
bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from bacterial cells using the RNeasy mini kit (5 mL culture) and RNeasy maxi kit (50 mL culture) (Qiagen). RNA concentrations were measured using a Nanodrop spectrometer (ThermoScientific) ...
-
bioRxiv - Genetics 2020Quote: ... 1 µl 1 mg/ml Carrier RNA (QIAGEN), 1 µl 10% SDS ...
-
bioRxiv - Molecular Biology 2020Quote: ... and then with 0.1 mg/mL RNaseA (Qiagen) for 2h at 37°C ...
-
bioRxiv - Genomics 2020Quote: ... using 10X (2.5 ml) of PB buffer (Qiagen) following the manufacturers’ instructions with the only change being a 10 minute incubation at 37 degrees prior to the final elution spin and eluted in 100 μl of EB buffer (QIAGEN) ...
-
bioRxiv - Microbiology 2019Quote: ... 1 ml RLT buffer (Qiagen RNA Isolation Kit) + 10 μl β-mercaptoethanol was added and vortexed and a phenol chloroform extraction followed by an ethanol precipitation was carried out ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 100 μg/mL of RNase A (Qiagen). Cell death was assessed using flow cytometric analysis of Annexin V and propidium iodide staining according to the manufacturer’s instructions (eBioscience) ...
-
bioRxiv - Biochemistry 2020Quote: ... 3-4 mL of Ni-NTA resins (Qiagen) were applied on the gravity column ...
-
bioRxiv - Plant Biology 2021Quote: ... followed by proteinase K treatment (200ug/ml; Qiagen) at 50°C overnight ...
-
bioRxiv - Microbiology 2021Quote: ... 0.4 ul DNase A (100 mg/ml, Qiagen), 2 ul 1% IGEPAL CA-630 ...
-
bioRxiv - Microbiology 2021Quote: ... 0.6 ul RNase A (100 mg/ml, Qiagen), 3 ul forward and reverse primer mix (2.5 uM for each) ...
-
bioRxiv - Microbiology 2021Quote: ... 5 µL Proteinase K (20 mg/mL, Qiagen) and 100 µl 10% SDS (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... resuspended in 3 ml RNAprotect Bacteria Reagent (Qiagen), divided into 3 × 1 ml aliquots (3 technical replicates) ...
-
bioRxiv - Microbiology 2022Quote: ... in 5-ml polypropylene columns (no. 34964; Qiagen), washed with 50 mM Na2HCO3 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10 ml ATL lysis buffer (Qiagen, Hilden, Germany) supplemented with 2% Proteinase K (20 mg/ml ...
-
bioRxiv - Microbiology 2021Quote: ... in a 5 ml tube (Qiagen, DNAse/RNAsefree). The sediment samples were kept at 4 °C until the next day and then stored at −80 °C ...
-
bioRxiv - Developmental Biology 2022Quote: ... 0.2 μL of 20 mg/ml protease (Qiagen) was added ...
-
bioRxiv - Microbiology 2020Quote: ... 10 µl proteinase K (>600 mAU/mL, Qiagen) and 5 µl 20% sodium dodecyl sulfate solution were added ...
-
bioRxiv - Microbiology 2019Quote: ... 4 µg/mL DNase I (QIAgen, Hilden, Germany), 2 µg/mL RNase A (QIAgen) ...
-
bioRxiv - Physiology 2021Quote: ... 10μl Proteinase K (20mg/ml; Qiagen, Manchester, UK) and RNAse-free water were added (final volume of 900μl) ...