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Citations for Qiagen :
1901 - 1950 of 5860 citations for rno mir 542 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... cDNA was retrotranscribed from total RNA extracted using the Omniscript RT kit (Qiagen). For mitochondrial transcripts CoI and Nd4 ...
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bioRxiv - Microbiology 2021Quote: ... For reverse transcription and synthesis of complementary DNA the Omniscript RT Kit (Qiagen) using random hexamers (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using the Exiqon miRCURY LNA Universal RT microRNA kit (Qiagen). qPCR for individual miR-17∼92 members was performed using miRCURY LNA probes and miRCURY LNA SYBR Green PCR Kit (Qiagen).
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bioRxiv - Cancer Biology 2021Quote: cDNA synthesis was carried out using the miRCURY LNA RT Kit (Qiagen #339340) from human purified EVs or plasma RNA (10 ng ...
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bioRxiv - Bioengineering 2021Quote: ... The cDNA was added to RT² SYBR Green ROX qPCR Mastermix (Qiagen 330520) and the solution was added to a Custom RT2 PCR Array 384-well plate and qPCR program was run using QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems 4485701) ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was reverse transcribed using Omniscipt TM RT kit (Qiagen, US. Catalog# 205113) and the complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis for miRNA was done with the miRCURY LNA RT kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: RNA conversion to cDNA was performed using the miRCURY LNA RT Kit (Qiagen, Cat No ...
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bioRxiv - Neuroscience 2020Quote: ... and each sample was reverse transcribed using miScript II RT Kit (Qiagen, #218161). qRT-PCR reactions were performed with miScript SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: The RT-qPCR was performed on site using a RotorGene 5plex platform (Qiagen). Primers and Taqman dual labelled probes targeting Mtb and the internal control were procured from Eurofin Genomics ...
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bioRxiv - Immunology 2020Quote: ... The RNA was reverse transcribed to cDNA using the miScript RT kit (Qiagen). miRNAs were quantified using miScript Primer Assays (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... mRNA reverse transcription (RT) was performed using the QuantiTect reverse transcription kit (Qiagen) in accordance with the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... 18 µl of Omniscript enzyme at 4 U/µl (Omniscript RT Kit, Qiagen), and 37 µl water ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was prepared with reverse transcriptase using an Omniscript RT kit (205113, Qiagen) following the standard protocol provided ...
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bioRxiv - Plant Biology 2024Quote: ... The cDNA was then synthesized with an Omniscript Reverse Transcription (RT) Kit (QIAGEN). Quantitative PCR reactions were carried out in a CFX Connect Real-time PCR detection system (BioRad ...
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bioRxiv - Neuroscience 2022Quote: ... PCR Buffer 10x (Qiagen), 0.2 mM dNTPs and 400 mM primers ...
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bioRxiv - Plant Biology 2023Quote: ... PCR purification kit (QIAGEN) was used for DNA purification after proteinase K treatment for 2 hours ...
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bioRxiv - Plant Biology 2023Quote: ... 1X PCR buffer (Qiagen), 0.5 mM of each primer ...
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bioRxiv - Cell Biology 2024Quote: ... PCR Buffer 10x (Qiagen), 0.2 mM dNTPs and 400 mM primers ...
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bioRxiv - Molecular Biology 2021Quote: ... the PCR products were held at 4°C until PCR purification using the QIAquick PCR purification kit (#28106, Qiagen, Hilden, Germany). DNA concentrations were determined using NanoDrop 2000 (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... and real-time reverse transcription quantitative PCR (qRT-PCR) was performed using QuantiNova™ SYBR Green PCR Kit (Qiagen, Hilden, Germany). Primers were synthesized by IDT Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... The primers were designed by use of the Pyromark Assay Design Software package (Qiagen), and 10 CpG sites of the FOXP3 intron region were selected for quantitative DNA methylation analysis ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was then performed with specific primers for mouse IFN-α11 (PPM03050B-200, Qiagen), mouse β-actin (PPM02945B-200 ...
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bioRxiv - Neuroscience 2021Quote: ... These genes were compared against the housekeeping gene HPRT1 (Cat No. QT00199640, Qiagen Primer) and relative gene expression was quantified using the 2^ddCT method ...
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bioRxiv - Cancer Biology 2020Quote: ... All data were normalized to the housekeeping gene 18S (18S QuantiTect Primer Assay; Qiagen).
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bioRxiv - Immunology 2020Quote: Random-primer reverse transcription was done using RT2 First Strand kits (Qiagen; Valencia, CA), including a genomic DNA removal treatment ...
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bioRxiv - Cancer Biology 2022Quote: ... TBP and GAPDH were proprietary primers that were designed by Qiagen (Qiagen, Hilden, Germany). The samples were then subjected to quantitative Real-Time PCR (RT-qPCR ...
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bioRxiv - Genetics 2022Quote: ... Sequencing was performed using the sequencing primer TGTAGGATGAAGTCTTACA and PyroMark Gold Q24 Reagents (Qiagen) according to the manufacturer’s instructions on a PyroMark Q24 pyrosequencer (Qiagen).
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bioRxiv - Biochemistry 2022Quote: ... IL −6 and TNFα mRNA were detected by validated QuantiTect primer assays 144 (Qiagen).
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bioRxiv - Genetics 2023Quote: ... with biotin-labeled methylation-specific primers designed with Pyromark Assay Design Software 2.0 (Qiagen). The primer sequences are shown in Supplemental Table S1 ...
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bioRxiv - Microbiology 2024Quote: Mouse cDNA was amplified with gene-specific primers and HotStarTaq Master Mix Kit (Qiagen) (Table 1) ...
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Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... including DNase I digestion via the RNase-Free DNase Set to remove traces of genomic DNA (Qiagen, Hilden, Germany). Quality and quantity of the isolated RNA was examined with the Fragment Analyzer (Advanced Analytical Technologies GmbH ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction was performed using 100/G Genomic-tips together with a Genomic DNA buffer set as described in the manufacturer’s instructions (Qiagen). After precipitation ...
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bioRxiv - Microbiology 2022Quote: ... followed by additional purification using RNeasy spin columns with DNase treatment according to the manufacturer’s instructions (Rneasy Mini Kit; RNAse-Free DNase Set; QIAGEN). RNA was reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was further purified by an on-column DNAse digestion using the RNase-Free DNase Set (Qiagen, Cat# 79254) and two rounds of washes using the RNAeasy Mini Kit (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... Each dataset was mapped to the to the predicted gene sets (NCBI Annotation Release 100) using CLC Genomics (Qiagen). Reads were mapped with the following parameters ...
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA was subjected to on-column DNase digestion with the RNase-free DNase set (Qiagen, Cat. No. 79254), cDNA synthesis and RT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... and an on-column DNase I digest was performed with the Qiagen RNase-Free DNase Set (Qiagen, Hilden, Germany). RNA was eluted in 50 μl of RNase-free molecular biology grade water and DNA was removed by incubation with 1 μl TURBO DNase (2 U/μl ...
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bioRxiv - Immunology 2021Quote: ... lysed with Qiazol lysis reagent and total RNA was purified using miRNeasy kit and RNAse-free DNAse set according to the manufacturer’s instructions (Qiagen). RNA quality was checked using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Genetics 2020Quote: ... according to the manufacturer’s instructions including an on-column DNaseI digestion step using the RNase-free DNase Set (Qiagen). First- strand cDNA synthesis and PCR reaction was performed as previously described.45 Exonspanning primers (Eurofins Genomics ...
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bioRxiv - Systems Biology 2021Quote: ... RNA-isolation for NGS applications was performed according to manufacturer’s protocol (Qiagen RNeasy mini kit, RNase free DNase set). For detection of protein abundance by western blotting ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was performed using 100/G Genomic-tips together with a Genomic DNA buffer set as described in the manufacturer’s instructions (Qiagen). After precipitation ...
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bioRxiv - Genomics 2021Quote: ... according to the manufacturer’s instructions with the addition of an on-column DNase digestion step using the RNase-free DNase Set (QIAGEN). Reverse transcription was performed using the QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... Further RNA extraction was performed according to the manufacturer’s instructions including an on-column DNase digestion using the RNase-Free DNase Set (Qiagen). RNA purity and quantity was measured using the Nanodrop (Nanodrop Technologies ...
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bioRxiv - Genetics 2022Quote: ... DNase digestion and RNA clean-up were performed using RNeasy Mini kit and RNase-Free DNase set (Qiagen, MD), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from bacterial strains using a Qiagen Genomic DNA Buffer Set (Qiagen, Germany; Cat. No. 19060) and Qiagen genomic tip 100/G gravity-flow ...
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bioRxiv - Microbiology 2023Quote: ... was applied and a DNase digestion was carried out by using the RNase-Free DNase Set (Qiagen, Hilden, Germany).
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bioRxiv - Neuroscience 2023Quote: ... Total RNA extraction and DNase treatment were carried out using miRNeasy Micro Kit and RNase-free DNase set according to the manufacturer’s instructions (QIAGEN).
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bioRxiv - Developmental Biology 2023Quote: ... qPCR reactions were set up in duplicate in the Rotor-Gene Q thermocycler 5 plex (Qiagen, Germantown, MD, USA). Primers reported here were designed using Primer BLAST (https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ...
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bioRxiv - Neuroscience 2023Quote: ... following the manufacturer’s instructions including DNase I treatment using RNase-Free DNase Set and on-column protocol (QIAGEN, USA). The isolated total RNAs were aliquoted for both RNA-seq and qRT-PCR.