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Citations for Qiagen :
1901 - 1950 of 6287 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were immediately lysed in RLT Plus buffer (Qiagen) and delivered to the University of Rochester Genomics Research Center for sequencing and analysis.
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bioRxiv - Immunology 2023Quote: ... cells were sorted directly into RNeasy Lysis Buffer (Qiagen). RNA was extracted and isolated from samples using RNeasy Micro Kit (74004 ...
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bioRxiv - Microbiology 2023Quote: ... 5×105 cells were lysed in RLT buffer (QIAGEN) with 10 μL of β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed and passed through QIAShredder column (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and cells were disrupted with a TissueLyse LT (Qiagen) for 5 mins at 50Hz ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed using Buffer RLT (Cat. # 79216; Qiagen) and stored at −80°C ...
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bioRxiv - Genetics 2023Quote: ... Cell lysate was applied to QIAshredder columns (QIAGEN, #1011711). Samples were incubated at room temperature for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... the cells were resuspended in TCL lysis buffer (Qiagen) supplemented with 1% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in 20 mL P1 buffer (Qiagen) with 100 μg/mL RNaseA and 0.5% SDS ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were transfected using HiPerFect Transfection Reagent (QIAGEN) with 5 nM of TGN46 siRNA (oligo name ...
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bioRxiv - Immunology 2024Quote: ... KPctrl and KPneo cells using RNeasy Mini kit (Qiagen) (3 samples/condition ...
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bioRxiv - Microbiology 2021Quote: Whole genome amplification of single sorted cells was carried out by multiple displacement amplification with the REPLI-g single cell kit (Qiagen, Cat. No. 150345), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Genomic DNA preparation and sequencing: Genomic DNA (gDNA) was isolated using the Blood & Cell Culture DNA Mini (<5e6 cells) Kits (Qiagen, cat. no. 13323) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Pathways significantly affected in non-T cell-inflamed or non-T cell-inflamed-enriched mutated genes were identified by IPA® (QIAGEN Inc., Germany).
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bioRxiv - Cell Biology 2021Quote: ... extracted from A549 cells (RIKEN Cell Bank, Ibaraki, Japan; authenticated) and purified with a high-purity RNA extraction kit (Qiagen Japan, Tokyo, Japan).
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bioRxiv - Genomics 2021Quote: ... Nuclei pellets and single cells were used to perform WGA using REPLI-g Advanced DNA single cell kit (QIAGEN; Cat No./ID: 150363) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from the mouse cells/tissues and human cell lines using the RNeasy® Mini Kit (Qiagen Inc., Germantown, MD). For human platelets and MEG-01 cells ...
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bioRxiv - Cell Biology 2023Quote: A Notch pathway transcriptional output reporter cell line was generated by infecting MCF10A cells with Cignal Lenti RBP-Jk Reporter (luc) lentiviral particles (Qiagen, Cat #: CLS-014L) following manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... was isolated using QIAamp DNA Blood Maxi (3x107 - 1x108 cells) or Midi (5x106 - 3x107 cells) kits following protocol directions (Qiagen, #51192 and #51183). gDNA precipitation was then used to concentrate the DNA ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in Propidium Iodide (PI) staining solution (1x PBS supplemented with 50 µg/mL PI, Invitrogen; 100 µg/mL RNase A, Qiagen; and 2 mM MgCl2) and incubated for 30 min at room temperature in the dark) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 ng of pFLAG expression vectors or empty plasmids and 50 ng psiCheck-2 plasmid were transfected using Effectene transfection reagent (Qiagen, Netherlands, Cat. No. 301425), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 20–30 mg of tissue was taken and placed into a 2 mL tube with 600 µL of RLT buffer with 1% β–mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Hilden, Germany: #69989). Tissues were then dissociated using a Qiagen TissueLyser II (Qiagen ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions were quenched at different time points (2, 5, 7, 10 and 20 minutes) by the addition of 250 μL of PB buffer (Qiagen QIAquick PCR Purification Kit) supplemented with 10 mM of EGTA ...
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bioRxiv - Molecular Biology 2021Quote: ... Both the input and IP samples were incubated at 50°C for 2 hr and then cleaned up using the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany, Cat # 28104) and eluted in 35 μL of water.
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bioRxiv - Systems Biology 2019Quote: RNA was extracted from a total of 17 samples from Experiment 2 with the AllPrep PowerFecal DNA/RNA Kit (Qiagen USA, Cat. No. 80244). The 17 samples included 7 target samples taken during antibiotic treatment (4 untreated and 3 non-responder ...
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bioRxiv - Pathology 2021Quote: ... 20 – 30 mg of tissue were placed into a 2 ml tube with 600 µl of RLT buffer with 1% β-mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Valencia, CA; #69989). Tissues were then dissociated using a Qiagen TissueLyser II (Qiagen ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... placed into a 2 mL tube with 600 μL of RLT buffer with 1% β–mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Hilden, Germany: #69989), then dissociated using a Qiagen TissueLyser II (Qiagen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... with 5 µl proteinase K (20 mg/ml) and lysed overnight at 56°C under shaking in Cell Lysis Solution (Qiagen Gentra Puregen Cell kit) with proteinase K (20 mg/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... Plantaris tendons or cultured cells were homogenized in QIAzol (Qiagen) and RNA was purified with a miRNeasy Micro Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... and Trp53.3 CT26Cas9GFP cells using a DNA extraction kit (Qiagen) per the manufacturer’s direction ...
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bioRxiv - Cell Biology 2019Quote: ... infected cell lysates were prepared using RLT buffer (Qiagen, 79216) prior to RT-qPCR analysis according to a validated BSL-4 procedure ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were loaded onto Ni-NTA agarose beads (Qiagen) and incubated with orbital rotation overnight at RT ...
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bioRxiv - Cell Biology 2020Quote: Cultured cells were lysed with Qiagen buffer RLT (Qiagen, 79216) supplemented with 1% β-mercaptoethanol (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... the pelleted cells were resuspended in QIAzol (cat. 79306, Qiagen), mixed with equal volume of Ethanol 100% and transferred to Zymo-Spin™ IICR Column ...
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bioRxiv - Biochemistry 2022Quote: ... cells were used to isolate mRNA (RNeasy Mini Kit, Qiagen) that was reverse transcribed into cDNA (AffinityScript ...
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bioRxiv - Cancer Biology 2022Quote: T47D cells were isolated using the RNeasy Isolation Kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then lysed in RLT Buffer (QIAGEN, Hilden, Germany) and total RNA was extracted using the Agencourt RNAdvance Tissue kit (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: Cells were lysed in QIAzol Lysis reagent (Qiagen, Valencia, CA) and total RNA was extracted using the miRNeasy mini kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell transfections were performed using the Effectene Transfection Reagent (Qiagen). Two days after transfection ...
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bioRxiv - Molecular Biology 2020Quote: RNA was isolated from INS1 cells using RNeasy kit (Qiagen) and from islets using TRIzol reagent (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Cell transfections were carried out using Effectene Transfection Reagent (QIAGEN) according to manufacturer’s instructions.
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bioRxiv - Systems Biology 2020Quote: ... cells were resuspended in 1 ml buffer RLT from Qiagen RNeasy kit ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were isolated using RNA RNeasy Miniprep Kit (Qiagen #74104) following the manufacturer’s protocol with DNase treatment ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were isolated using the RNeasy Mini Kit (Qiagen #74104) following the manufacturer’s protocol with DNase treatment.
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bioRxiv - Microbiology 2019Quote: ... coli cells and purified using the QiaQuick Miniprep kit (Qiagen) and quantified via Qubit Fluorometric Quantitation (ThermoFisher) ...
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bioRxiv - Microbiology 2019Quote: ... The cell solution/suspension? was homogenised using QIAshredder columns (Qiagen) and centrifugation at 145000 RPM for two minutes at room temperature using MiniSpin tabletop centrifuge (Eppendorf) ...
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bioRxiv - Immunology 2021Quote: ... harvested cells were lysed in RLT buffer (Qiagen, Hilden, Germany) containing 1%β-mercaptoethanol (Roth ...
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bioRxiv - Immunology 2021Quote: ... 1,000 cells were sorted into 5μL of Buffer TCL (QIAGEN) supplemented with 1% 2-mercaptoethanol ...
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bioRxiv - Biochemistry 2021Quote: ... or pQE30 vectors (expressed in M15 E. coli cells (Qiagen)) ...
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bioRxiv - Neuroscience 2021Quote: HEK 293 cells were transfected using PolyFect Transfection Reagent (Qiagen) according to manufacturer’s instructions ...