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Citations for Qiagen :
1901 - 1950 of 3720 citations for E 6 2 6 6 Trimethylcyclohex 2 en 1 yl hex 5 ene 2 4 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... 1.28 M sucrose, 40 mM Tris-Cl, pH 7.5, 20 mM MgCl2, and 4% Triton X-100; QIAGEN) and then digested by digestion buffer (QIAGEN buffer G2 ...
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bioRxiv - Microbiology 2022Quote: ... 14 μL of filtered supernatant was combined with 4 μL of RDD buffer (Qiagen, RNase-Free DNase Set) and 2 μL of DNase I and dissolved as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... for 45 min at 4°C and the supernatant was mixed with 3 mL Ni-NTA agarose (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
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bioRxiv - Cell Biology 2023Quote: ... 10 minutes 4°C and the supernatant was incubated with pre-equilibrated Ni-NTA agarose beads (Qiagen, #1018244) with shaking on ice for 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: We also used the RT2 Profiler PCR array mouse fibrosis (cat. #PAMM 120-ZE-4) kit from Qiagen to detect the expression of 84 fibrosis-related genes ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 sections 10 µm thick were placed in a chilled Eppendorf tube and RNA extraction protocol from Qiagen was performed (Rneasy FFPE Kit 73504 ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from cSCC cell lines (n = 8) and NHEKs (n = 4) using miRNeasy Mini Kit (Qiagen), and the RNA-seq analysis was performed using Illumina HiSeq2500 system using paired-end sequencing chemistry with 100bp read length (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria were centrifuged at 4°C and washed with pre-cold PBS containing RNA stabilization solution (RNAprotect, Qiagen) before flash freezing of the pellets and storage at -80°C ...
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bioRxiv - Biochemistry 2024Quote: ... for 45 min at 4°C and the supernatant was mixed with 3 mL Ni-NTA agarose (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Genetics 2020Quote: ... 1 µl 1 mg/ml Carrier RNA (QIAGEN), 1 µl 10% SDS ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 nM of each primer and 5 µl of SYBR Green QuantiFast RT-qPCR master mix (Qiagen). Primers used were ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 mg of skin was homogenized in 20% 0.4M DTT in RLT Lysis Buffer (Qiagen, Hilden, Germany). The RNA was isolated using the RNeasy Plus Micro Kit (cat ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were isolated from 5 mL of overnight liquid culture using QIAprep Spin Miniprep Kit (Qiagen, Germany) and the presence of mutations was confirmed by Sanger Sequencing.
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded onto a gravity column containing 5 ml of 50% Ni-NTA resin (Qiagen) pre-equilibrated with 40 ml lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: Total RNA from 5×105 FACS sorted p7 MPs was isolated using the RNeasy Micro Kit (Qiagen). RNA quantity and quality was tested on a Qubit®Fluorometer (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... matching a sequence in intron 22 of the HTT gene (5’-TAATACGTAAGTGTCACAA-3’, custom synthesized by Qiagen) was delivered by intracerebroventricular (ICV ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng of each RNA was reverse transcribed to cDNA using the miRCURY reverse transcription kit (Qiagen). Qiagen’s miRCURY Locked Nucleic Acid (LNA ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from approximately 5×105 CD133 purified progenitors using an RNeasy micro kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Condensin I was bound to Glutathione Sepharose 4B beads (Cytiva) in a 5 mL polypropylene column (Qiagen) using GST- tagged mSMC4 and eluted by PreScission Protease (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... and 5 units lysostaphin and mutanolysin for 30 minutes prior to beadbeating in the TissueLyser II (QIAGEN) for 2 x 3 minutes at 30 Hz ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from pools of 3-5 second leaves using the Plant RNAeasy kit (Qiagen) using manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 5 and 0.22 μm pore-size filters were processed using the DNeasy PowerWater Kit (QIAGEN, Hilden, Germany) following the manufacture instructions ...
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bioRxiv - Microbiology 2021Quote: ... and 5 units lysostaphin and mutanolysin for 30 minutes prior to beadbeating in the TissueLyser II (QIAGEN) for 2 x 3 minutes at 30 Hz ...
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bioRxiv - Genomics 2021Quote: DNA was extracted from the donors’ peripheral blood (5 ml) using DNeasy Blood & Tissue Kit from QIAGEN according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 300mM NaCl and 5% glycerol and the clarified lysate was loaded on a Ni-NTA column (Qiagen). Non-specifically bound proteins were removed by washing with lysis buffer containing 40mM imidazole and σA eluted with 150mM imidazole ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 cells were collected for genomic DNA extraction using red blood cell lysis solution (Qiagen, 28606).
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bioRxiv - Plant Biology 2022Quote: All PCR reactions were performed with the 5-Prime Hot Master Mix (Quantabio, QIAGEN, Beverly, MA, U.S.A.). All PCR products and pooled library were purified with CleanNGS beads (CleanNA ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from neuron-astrocyte co-cultures at 5 weeks using RNeasy Mini kit (74104, Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: On day 5 of dietary exposure RNA was extracted via the Qiagen RNeasy Mini Kit (Qiagen 74104). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Sorted cells were then centrifuged (300xg, 5 min) before RNA isolation using the RNeasy Mini Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was added to a 50 mL Falcon tube containing 5 ml Ni-NTA suspension (Qiagen) resuspended in solution A and left to settle on a tilting table for 1 hour at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was extracted and purified from 5 ml or 250 ml bacterial culture using Miniprep (Qiagen) or Maxiprep (Thermo-Scientific ...
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bioRxiv - Immunology 2023Quote: ... cells pooled from n = 3 - 5 mice / cohort) was isolated using the RNeasy Plus Mini Kit (Qiagen). Clariom S microarray analysis (Affymetrix ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was added to a 50 mL Falcon tube containing 5 ml Ni-NTA suspension (Qiagen) resuspended in 2 mL solution A and left to settle on a tilting table for 1 hour at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... at 300 cells per well into 96 well LoBind plates containing 5 μL of EB buffer (Qiagen). After sorting ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was passed through a 5 mL column volume (CV) of Ni-NTA agarose resin (Qiagen: 30250). The column was then washed with 10 CV of His binding buffer (50 mM NaH2PO4 ...
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bioRxiv - Neuroscience 2024Quote: ... The pellet was resuspended in 340μl of lysis buffer containing 5 mM EDTA and proteinase K (Qiagen) and incubated at 55°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... Tsg101 (Hs_TSG101_6) target sequence: 5′-CAG TTT ATC ATT CAA GTG TAA -3′ (QIAGEN, cat. no. SI02655184); Alix (Hs_PDCD6IP_5 ...
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bioRxiv - Bioengineering 2024Quote: ... Alix (Hs_PDCD6IP_5) target sequence: 5′-AAG AGC TGT GTG TTG TTC AAT -3′ (QIAGEN, cat. no. SI02655345); Negative Control siRNA ...
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bioRxiv - Microbiology 2024Quote: ... NGS libraries were constructed using Enzymatics 5× WGS fragmentation mix and WGS ligase reagents (Qiagen, Hilden, Germany), and then libraries were sequenced on HiSeq X Five platform in Macrogen Japan (Tokyo ...
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bioRxiv - Immunology 2024Quote: RNA was extracted from 5 × 106 T cells using the RNeasy® Mini Kit (Qiagen, Cat. 74106). RNA extraction was performed following the instructions from the “Purification of Total RNA from Animal Cells Using Spin Technology” protocol given in the RNeasy® Mini Handbook ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mm punch biopsies were excised from trunk nodules and immersed in 2.5 ml RNALater Reagent (Qiagen). Incisions were treated with iodine and oxytetracycline ...
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bioRxiv - Molecular Biology 2024Quote: Total RNAs were extracted from 5×105 cultured cells using an RNA isolation kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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Microbial iCLIP2: Enhanced mapping of RNA-Protein interaction by promoting protein and RNA stabilitybioRxiv - Molecular Biology 2024Quote: ... one steel bead (5 mm diameter) was added and placed into a pre-cooled TissueLyser Adapter (Qiagen). The samples underwent two rounds of cell lysis ...
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bioRxiv - Immunology 2024Quote: ... The digested mixture was then loaded onto a pre-equilibrated 5 mL Ni-NTA Superflow column (Qiagen) to remove the cleaved His-tag ...
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bioRxiv - Bioengineering 2024Quote: ... Gibco) with 5% (w/v) glucose (Fisher ChemicalTM) pH 7.4 and nuclease-free water (NF water, Qiagen). Then ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from ∼100,000 MSNs from 4 independent differentiations per experimental group according to the manufacturer’s protocol and purified using RNeasy columns (Qiagen). A total of 72 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was bound to Ni-NTA Agarose resin (Qiagen), washed with 10 CV of wash buffer (50 mM HEPES-KOH pH=7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared using a JA-20 rotor (18,000 rpm for 30 min at 4°C) and incubated with Ni-NTA superflow resin (Qiagen) for 30 min ...