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Citations for Qiagen :
1801 - 1850 of 2379 citations for 6 Oxa 7 azatricyclo 3.3.0.02 4 oct 7 ene 8 1 methylethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... V6.5 mESCs and K562 genomic DNA was purified from 1 million cells using the QIAamp micro kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Reverse transcription was performed with 1 µg of template RNA using the Qiagen QuantiTect reverse transcription kit (Qiagen) in a Bio-Rad T100TM Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was then purified from 500,000 to 1 million cells using the miRNeasy Micro kit (Qiagen GmbH) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... a minimum of 1 x106 cells per experimental group were processed using the RNeasy Mini kit (#74104; QIAGEN). The mRNA levels were measured with the NanoDrop (DS-11 ...
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bioRxiv - Physiology 2024Quote: ... using 350 microliter RLT buffer with 1% b-mercaptoethanol as a collection/lysis buffer (RNeasy micro kit, Qiagen). RNA was extracted following manufacturer’s instructions (RNeasy micro kit ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded using a peristaltic pump in 1 mL (bed volume) of Ni-NTA agarose (QIAGEN) packed in a Poly-Prep® Chromatography Column (Cat #731-1550 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA of PDOs grown in ‘Type 1’ culture medium was isolated according to the manufacturer’s protocol using the RNeasy Mini Kit (QIAGEN). Quality and quantity of the RNA samples and the libraries were measured with Agilent’s Bioanalyzer2100 and Invitrogen™ Qubit™ 3.0 Fluorometer ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNA was isolated following the manufacturer’s protocol and cDNA was synthesized from 1 μg RNA using the QuantiTekt Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed once with 1 % BSA and incubated with a solution containing 200 μg/ml RNase A (Qiagen) and 50 μg/ml Propodium Iodide (Sigma ...
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bioRxiv - Genetics 2021Quote: ... PCR 2 products were purified by electrophoresis with a 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen) and eluting with 30 μL water ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed with PBS and incubated at 37°C with a 1:100 dilution of RNase A (QIAGEN) in PBS for 3 hours ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 μL of primer mix (with a fluorescently labelled forward primer) at 0.2 μM and 1 μL QIAGEN Multiplex PCR Master Mix (QIAGEN). PCR cycling conditions were 95 °C for 10 min followed by 35 cycles of 95 °C for 30 s ...
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bioRxiv - Pathology 2022Quote: ... RNA was isolated from maternal and fetal tissues (Table 1) using QIAamp cador pathogen mini kit (Qiagen, Valencia, CA). ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen ...
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bioRxiv - Physiology 2022Quote: ... The tissue was homogenized for 1 min at 28.5 Hz in a TissueLyser (Qiagen TissueLyser II, Retsch GmbH, Germany) before samples were rotated end over end for 1 hour at 4°C and centrifuged at 18320g for 20 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were then resuspended in 500 μL of supernatant and stabilized with 1 mL of RNAprotect Bacteria Reagent (Qiagen). The RNeasy® Protect Bacteria Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: HIV-1 viral RNA was extracted from 20-50 ul of plasma using QIAamp Viral RNA mini kit (Qiagen). qRT-PCR was performed using a TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Genomics 2020Quote: ... the final clean-up was performed at 0.9:1 ratio of beads to PCR products and elution again done in 16μl EB (Qiagen, Germany). Samples were diluted to 8pM final concentration before sequencing on an Illumina Miseq (2×25nt).
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bioRxiv - Systems Biology 2019Quote: ... ∼20 mg frozen tissue was pulverised in chloroform-methanol (400 µl; 2:1 v/v) using a TissueLyser (Qiagen), then the mixture was sonicated for 10 minutes ...
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bioRxiv - Synthetic Biology 2019Quote: ... The PCR products were separated on a 1% agarose gel and purified using a QIAquick Gel Extraction Kit (Qiagen). The parts were assembled using the NEB HiFi DNA Assembly kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2019Quote: ... each collection lid was covered with 15μl of a 1:16 dilution of proteinase K in PKD buffer (Qiagen), incubated at room temperature for 5 min and subsequently snap frozen in dry ice and stored at −80°C overnight ...
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bioRxiv - Pathology 2020Quote: Total RNA was extracted from the BALF sample of patient 1 using the RNeasy Plus Universal Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... 750ng of the gDNA was labeled using DLE-1 enzyme and reaction mix followed by Proteinase K digestion (Qiagen). The DNA back bone was stained after drop dialysis ...
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bioRxiv - Plant Biology 2019Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using QuantiTect® Reverse Transcription kit (Qiagen, Germany).
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bioRxiv - Molecular Biology 2021Quote: HOS cells were cotransfected with a Rev/Env-encoding pSVIIIenv plasmid and a Tat-encoding plasmid at a 1:0.125 ratio using the Effectene transfection reagent (Qiagen). At 48 h after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed on cDNA dilutions of 1/20 in the presence of 7.5-pmol primers with QuantiTect SYBR Green PCR Master Mix (Qiagen) on a Lightcycler 480 (Roche) ...
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bioRxiv - Microbiology 2021Quote: Virus isolation was performed on tissues following homogenization in 1 mL DMEM using a TissueLyser (Qiagen, Germantown, MD, USA) and inoculating Vero E6 cells in a 96 well plate with 200 µL of 1:10 serial dilutions of the homogenate ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed according to the manufacturer’s recommendations on 1 μg of RNA with the QuantiTect Reverse Transcription Kit (Qiagen), which includes a genomic DNA–removal step ...
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bioRxiv - Microbiology 2020Quote: ... A total of OD600 = 0.3 in 1 ml of cells was pelleted and gDNA was extracted using DNeasy Blood & Tissue kit (Qiagen) using the Gram-negative bacterial protocol followed by quantification using Qubit ...
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bioRxiv - Plant Biology 2021Quote: ... ABI1 and ABI2 with primers listed in Supplemental Table 1 using the Rotor Gene SYBR Green PCR Kit (Qiagen) according to manufacturers’ protocol in a Rotor gene Q cycler (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... Fastq files were mapped to KSHV genome (GenBank accession no. AP017458 for SPEL, and NC_003409 for BCBL-1) by CLC Genome Workbench (version 14, Qiagen), and the coverage data were obtained using Integrative Genomics Viewer (version 2.50 ...
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bioRxiv - Microbiology 2020Quote: Each dissected compartment (legs and thoraxes) was homogenized individually 3 x 1 minute at 30 Hertz using TissueLyser (Qiagen) and glass beads (#11079110 ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... after which a second volume of 400μL a 1% β-mercaptoethanol in RLT buffer (AllPrep DNA/RNA Minikit, Qiagen) was added after steel bead removal ...
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bioRxiv - Plant Biology 2020Quote: ... 200 mg of freeze-dried and ground roots were mixed with 1.6 mL of 1 M perchloric acid and homogenized in a TissueLyser II (Qiagen) in microtubes containing 6 glass beads of 2.8 mm in diameter ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... 10% glycerol and 1 mM DTT) and recombinant TrcP was purified from clarified lysates using Ni-NTA resin (Qiagen) and gravity chromatography ...
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bioRxiv - Genetics 2022Quote: Total RNA was isolated from PWS and control INS-1 lines grown as above (7.5 mM glucose) by Trizol harvest and miRNeasy (Qiagen) column purification ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was washed three times with ice-cold PBS and suspended in 1 ml TRIzol™ reagent (QIAGEN). Instead ...
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bioRxiv - Molecular Biology 2022Quote: ... 1×104 cells of each sample were lysed in 100 µl of RLT Plus Lysis Buffer (Qiagen, Hilden, Germany) supplemented with 1% 2-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... and stored pellets at −80 °C for <1 week before RNA extraction using RNeasy Protect Bacteria Mini Kit (Qiagen) according to the manufacturer’s instructions (protocol #5) ...
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bioRxiv - Cell Biology 2022Quote: ... and were then incubated with or without triton-x (1% v/v) and with or without RNase-A (Qiagen #19101 ...
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bioRxiv - Cancer Biology 2022Quote: 1 μg of bulk genomic DNA was extracted from gastric tumors using the DNeasy Blood and Tissue Kit (Qiagen) and WES was conducted and sequenced by BGI ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of bulk genomic DNA was extracted from gastric tumors using the DNeasy Blood and Tissue Kit (Qiagen) and sonicated using the Covaris instrument ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA from 1-5 million total CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen) or phenol-chloroform and the DNA concentration was measured by Qubit High Sensitivity Kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... miR-67 (miR control) or empty vector at a ratio of 1:3 using a lipofection protocol (Effecten, Qiagen) and processed at DIV15 ...
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bioRxiv - Microbiology 2020Quote: Airway epithelia cultured on 6.5-mm Transwell membranes were washed and treated for 1 minute at RT with RLT buffer (Qiagen) with 1% β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... cancer-associated fibroblasts (CAFs) and C666-1 cells using RNeasy Mini kit per the manufacturer’s instructions (Qiagen, Hilden, Germany). RNA integrity number (RIN ...
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bioRxiv - Biochemistry 2021Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were confirmed by agarose gel electrophoresis using 0.8-1% agarose and purified using PCR purification kit (Qiagen). In case of NS1 gene ...
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bioRxiv - Microbiology 2021Quote: ... with sequences and concentrations listed in Table 1 in combination with QuantiTect Multiplex PCR Kit (Qiagen, Germantown, MD, USA). PMAxx (Biotium ...
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bioRxiv - Microbiology 2022Quote: Frozen tissue was partially thawed and submerged in lysis buffer containing 1% β-mercaptoethanol and 0.5% Reagent DX (Qiagen) before tissues were homogenised together with TissueRupture (Qiagen) ...